The telomeric DNA damage response occurs in the absence of chromatin decompaction.

abstract：:We show the intracellular localization of the Escherichia coli replication origin (oriC) and chromosome terminus during the cell division cycle by FISH. In newborn cells, oriC is localized at the old-pole-proximal nucleoid border and the terminus at the new-pole-proximal nucleoid border. One copy of replicated oriC mi...

journal_title：Genes & development

authors： Niki H,Hiraga S

更新日期：1998-04-01 00:00:00

abstract：:The X-inactive-specific transcript (Xist) locus is a cis-acting switch that regulates X chromosome inactivation in mammals. Over recent years an important goal has been to understand how Xist is regulated at the initiation of X inactivation. Here we report the analysis of a series of targeted mutations at the 5' end o...

journal_title：Genes & development

authors： Nesterova TB,Johnston CM,Appanah R,Newall AE,Godwin J,Alexiou M,Brockdorff N

更新日期：2003-09-01 00:00:00

abstract：:Here we demonstrate that RNF4, a highly conserved small ubiquitin-like modifier (SUMO)-targeted ubiquitin E3 ligase, plays a critical role in the response of mammalian cells to DNA damage. Human cells in which RNF4 expression was ablated by siRNA or chicken DT40 cells with a homozygous deletion of the RNF4 gene displa...

journal_title：Genes & development

authors： Yin Y,Seifert A,Chua JS,Maure JF,Golebiowski F,Hay RT

更新日期：2012-06-01 00:00:00

abstract：:Noncoding RNA regulators have been implicated in almost all imaginable cellular processes. Here we review how regulatory small RNAs such as Spot42, SgrS, GlmY, and GlmZ and a cis-encoded ribozyme in glmS mRNA control sugar metabolism. Besides discussing the physiological implications, we show how the study of these mo...

journal_title：Genes & development

authors： Görke B,Vogel J

更新日期：2008-11-01 00:00:00

abstract：:X. laevis stage VI oocytes respond differently from unfertilized eggs when injected with the genes for X. laevis embryonic U1 RNAs, xU1b1, and xU1b2. Upon maturation of oocytes into eggs, the efficiency of transcription decreases greatly and the ratio of xU1b1 to xU1b2 RNA transcription changes. Moreover, DNA replicat...

journal_title：Genes & development

authors： Lund E,Bostock CJ,Dahlberg JE

更新日期：1987-03-01 00:00:00

abstract：:The transcription factor TFIIB plays a central role in preinitiation complex assembly, providing a bridge between promoter-bound TFIID and RNA Polymerase II. TFIIB possesses sequence-specific DNA-binding ability and interacts with the TFIIB-recognition element (BRE), present in many promoters. Here we show that the BR...

journal_title：Genes & development

authors： Evans R,Fairley JA,Roberts SG

更新日期：2001-11-15 00:00:00

abstract：:We have identified protein kinase C-zeta (PKC-zeta) as a novel suppressor of neoplastic transformation caused by the v-raf oncogene. PKC-zeta overexpression drastically retards proliferation, abolishes anchorage-independent growth, and reverts the morphological transformation of v-raf-transformed NIH-3T3 cells. The mo...

journal_title：Genes & development

authors： Kieser A,Seitz T,Adler HS,Coffer P,Kremmer E,Crespo P,Gutkind JS,Henderson DW,Mushinski JF,Kolch W,Mischak H

更新日期：1996-06-15 00:00:00

abstract：:We report on the function of the human ortholog of Saccharomyces cerevisiae Rif1 (Rap1-interacting factor 1). Yeast Rif1 associates with telomeres and regulates their length. In contrast, human Rif1 did not accumulate at functional telomeres, but localized to dysfunctional telomeres and to telomeric DNA clusters in AL...

journal_title：Genes & development

authors： Silverman J,Takai H,Buonomo SB,Eisenhaber F,de Lange T

更新日期：2004-09-01 00:00:00

abstract：:In mitosis, the spindle checkpoint protein Mad2 averts aneuploidy by delaying anaphase onset until chromosomes align. Here we show that depletion of Mad2 in meiosis I mouse oocytes induced an increased incidence of aneuploidy. Proteolysis of cyclin B and securin commenced earlier in Mad2-depleted oocytes, resulting in...

journal_title：Genes & development

authors： Homer HA,McDougall A,Levasseur M,Yallop K,Murdoch AP,Herbert M

更新日期：2005-01-15 00:00:00

abstract：:The fission yeast telomerase RNA (TER1) precursor harbors an intron immediately downstream from its mature 3' end. Unlike most introns, which are removed from precursor RNAs by the spliceosome in two sequential but tightly coupled transesterification reactions, TER1 only undergoes the first cleavage reaction during te...

journal_title：Genes & development

authors： Kannan R,Hartnett S,Voelker RB,Berglund JA,Staley JP,Baumann P

更新日期：2013-03-15 00:00:00

abstract：:The assembly of a nervous system requires the extension of axons and dendrites to specific regions where they are matched with appropriate synaptic targets. Although the cues that guide long-range outgrowth have been characterized extensively, additional mechanisms are required to explain short-range guidance in neura...

journal_title：Genes & development

authors： Dow E,Siletti K,Hudspeth AJ

更新日期：2015-05-15 00:00:00

abstract：:Genetic and cytologic data from Saccharomyces cerevisiae and mammals implicate the Mre11 complex, consisting of Mre11, Rad50, and Nbs1, as a sensor of DNA damage, and indicate that the complex influences the activity of ataxia-telangiectasia mutated (ATM) in the DNA damage response. Rad50(S/S) mice exhibit precipitous...

journal_title：Genes & development

authors： Morales M,Theunissen JW,Kim CF,Kitagawa R,Kastan MB,Petrini JH

更新日期：2005-12-15 00:00:00

abstract：:The allosteric and torpedo models have been used for 30 yr to explain how transcription terminates on protein-coding genes. The former invokes termination via conformational changes in the transcription complex and the latter proposes that degradation of the downstream product of poly(A) signal (PAS) processing is imp...

journal_title：Genes & development

authors： Eaton JD,Francis L,Davidson L,West S

更新日期：2020-01-01 00:00:00

abstract：:Metastasis accounts for the vast majority of cancer-related deaths, yet the molecular mechanisms that drive metastatic spread remain poorly understood. Here we report that Tks5, which has been linked to the formation of proteolytic cellular protrusions known as invadopodia, undergoes an isoform switch during metastati...

journal_title：Genes & development

authors： Li CM,Chen G,Dayton TL,Kim-Kiselak C,Hoersch S,Whittaker CA,Bronson RT,Beer DG,Winslow MM,Jacks T

更新日期：2013-07-15 00:00:00

abstract：:Class switch recombination (CSR) diversifies antibodies for productive immune responses while maintaining stability of the B-cell genome. Transcription at the immunoglobulin heavy chain (Igh) locus targets CSR-associated DNA damage and is promoted by the BRCT domain-containing PTIP (Pax transactivation domain-interact...

journal_title：Genes & development

authors： Starnes LM,Su D,Pikkupeura LM,Weinert BT,Santos MA,Mund A,Soria R,Cho YW,Pozdnyakova I,Kubec Højfeldt M,Vala A,Yang W,López-Méndez B,Lee JE,Peng W,Yuan J,Ge K,Montoya G,Nussenzweig A,Choudhary C,Daniel JA

更新日期：2016-01-15 00:00:00

abstract：:A novel cysteine-rich motif, named LIM, has been identified in the homeo box genes lin-11, Isl-1, and mec-3; the mec-3 and lin-11 genes determine cell lineages in Caenorhabditis elegans. We isolated LIM class homeo box genes from Xenopus laevis that are closely related to lin-11 and mec-3 in the LIM and homeo domains....

journal_title：Genes & development

authors： Taira M,Jamrich M,Good PJ,Dawid IB

更新日期：1992-03-01 00:00:00

abstract：:The labial (lab) gene of Drosophila melanogaster is necessary for the proper development of the embryonic (larval) and adult head. We have identified the lab transcription unit within the proximal portion of the Antennapedia Complex (ANT-C) by mapping the molecular lesions associated with chromosomally rearranged lab ...

journal_title：Genes & development

authors： Diederich RJ,Merrill VK,Pultz MA,Kaufman TC

更新日期：1989-03-01 00:00:00

abstract：:Although the transporter-like protein Patched (Ptc) is genetically implicated in reception of the extracellular Hedgehog (Hh) protein signal, a clear definition of the Hh receptor is complicated by the existence of additional Hh-binding proteins and, in Drosophila, by the lack of physical evidence for direct binding o...

journal_title：Genes & development

authors： Zheng X,Mann RK,Sever N,Beachy PA

更新日期：2010-01-01 00:00:00

abstract：:The proteasome is well known for its regulation of the cell cycle and degradation of mis-folded proteins, yet many of its functions are still unknown. We show that RPN11, a gene encoding a subunit of the regulatory cap of the proteasome, is required for UV-stimulated activation of Gcn4p target genes, but is dispensabl...

journal_title：Genes & development

authors： Stitzel ML,Durso R,Reese JC

更新日期：2001-01-15 00:00:00

abstract：:Gene duplication and divergence is a major driver in the emergence of evolutionary novelties. How variations in amino acid sequences lead to loss of ancestral activity and functional diversification of proteins is poorly understood. We used cross-species functional analysis of Drosophila Labial and its mouse HOX1 orth...

journal_title：Genes & development

authors： Singh NP,De Kumar B,Paulson A,Parrish ME,Zhang Y,Florens L,Conaway JW,Si K,Krumlauf R

更新日期：2020-12-01 00:00:00

abstract：:The enzyme telomerase, which elongates chromosome termini, is a critical factor in determining long-term cellular proliferation and tissue renewal. Hence, even small differences in telomerase levels can have substantial consequences for human health. In budding yeast, telomerase consists of the catalytic Est2 protein ...

journal_title：Genes & development

authors： Tucey TM,Lundblad V

更新日期：2014-10-01 00:00:00

abstract：:Medulloblastoma (MB) is the most common pediatric CNS malignancy. We identify EAG2 as an overexpressed potassium channel in MBs across different molecular and histological subgroups. EAG2 knockdown not only impairs MB cell growth in vitro, but also reduces tumor burden in vivo and enhances survival in xenograft studie...

journal_title：Genes & development

authors： Huang X,Dubuc AM,Hashizume R,Berg J,He Y,Wang J,Chiang C,Cooper MK,Northcott PA,Taylor MD,Barnes MJ,Tihan T,Chen J,Hackett CS,Weiss WA,James CD,Rowitch DH,Shuman MA,Jan YN,Jan LY

更新日期：2012-08-15 00:00:00

abstract：:Sox10 is a high-mobility-group transcriptional regulator in early neural crest. Without Sox10, no glia develop throughout the peripheral nervous system. Here we show that Sox10 is restricted in the central nervous system to myelin-forming oligodendroglia. In Sox10-deficient mice progenitors develop, but terminal diffe...

journal_title：Genes & development

authors： Stolt CC,Rehberg S,Ader M,Lommes P,Riethmacher D,Schachner M,Bartsch U,Wegner M

更新日期：2002-01-15 00:00:00

abstract：:How dividing mammalian cells overcome blocks to DNA replication by DNA damage, depleted nucleotide pools, or template-bound proteins is unclear. Here, we show that the response to blocked replication requires BRCA2, a suppressor of human breast cancer. By using two-dimensional gel electrophoresis, we demonstrate that ...

journal_title：Genes & development

authors： Lomonosov M,Anand S,Sangrithi M,Davies R,Venkitaraman AR

更新日期：2003-12-15 00:00:00

abstract：:The YAP transcription coactivator has been implicated as an oncogene and is amplified in human cancers. Recent studies have established that YAP is phosphorylated and inhibited by the Hippo tumor suppressor pathway. Here we demonstrate that the TEAD family transcription factors are essential in mediating YAP-dependent...

journal_title：Genes & development

authors： Zhao B,Ye X,Yu J,Li L,Li W,Li S,Yu J,Lin JD,Wang CY,Chinnaiyan AM,Lai ZC,Guan KL

更新日期：2008-07-15 00:00:00

abstract：:The acetylation state of histones plays a central role in determining gene expression in chromatin. The reestablishment of the acetylation state of nucleosomes after DNA replication and chromatin assembly requires both deacetylation and acetylation of specific lysine residues on newly incorporated histones. In this st...

journal_title：Genes & development

authors： Meijsing SH,Ehrenhofer-Murray AE

更新日期：2001-12-01 00:00:00

abstract：:Previous work has shown that rRNA synthesis is strongly inhibited in yeast top1-top2 double mutants. Here, we show that inactivation of yeast topoisomerases can have paradoxical effects on transcription by RNA polymerase I. For example, transcription of ribosomal minigenes on extrachromosomal plasmids is greatly stimu...

journal_title：Genes & development

authors： Schultz MC,Brill SJ,Ju Q,Sternglanz R,Reeder RH

更新日期：1992-07-01 00:00:00

abstract：:Establishment of the steroid-producing Leydig cell lineage is an event downstream of Sry that is critical for masculinization of mammalian embryos. Neither the origin of fetal Leydig cell precursors nor the signaling pathway that specifies the Leydig cell lineage is known. Based on the sex-specific expression patterns...

journal_title：Genes & development

authors： Yao HH,Whoriskey W,Capel B

更新日期：2002-06-01 00:00:00

abstract：:Over a period of many months, Xenopus oocytes stockpile large quantities of tRNA for use during the first few hours of embryogenesis. To test the idea that these tRNAs are transcribed from one set of genes and that another set is used by somatic cells, we used synthetic oligonucleotides to analyze the sequence and ste...

journal_title：Genes & development

authors： Stutz F,Gouilloud E,Clarkson SG

更新日期：1989-08-01 00:00:00

abstract：:O(6)-Methylguanine ((Me)G) is a highly cytotoxic DNA modification generated by S(N)1-type methylating agents. Despite numerous studies implicating DNA replication, mismatch repair (MMR), and homologous recombination (HR) in (Me)G toxicity, its mode of action has remained elusive. We studied the molecular transactions ...

journal_title：Genes & development

authors： Mojas N,Lopes M,Jiricny J

更新日期：2007-12-15 00:00:00