The proteasome regulates the UV-induced activation of the AP-1-like transcription factor Gcn4.

abstract：:The ultraviolet (UV)-induced (6-4) pyrimidine-pyrimidone photoproduct [(6-4) PP] confers a large structural distortion in DNA. Here we examine in human cells the roles of translesion synthesis (TLS) DNA polymerases (Pols) in promoting replication through a (6-4) TT photoproduct carried on a duplex plasmid where bidire...

journal_title：Genes & development

authors： Yoon JH,Prakash L,Prakash S

更新日期：2010-01-15 00:00:00

abstract：:The association of proteins with the branch site region during pre-mRNA splicing was probed using a novel methodology to site-specifically modify the pre-mRNA with the photo-reagent benzophenone. Three sets of proteins were distinguished by the kinetics of their associations with pre-mRNAs, by their association with d...

journal_title：Genes & development

authors： MacMillan AM,Query CC,Allerson CR,Chen S,Verdine GL,Sharp PA

更新日期：1994-12-15 00:00:00

abstract：:The distal appendages (DAPs) of centrioles have been proposed to anchor cilia to the plasma membrane, but their molecular composition, assembly, and exact function in ciliogenesis remain poorly understood. Using quantitative centrosome proteomics and superresolution microscopy, we identified five DAP components, inclu...

journal_title：Genes & development

authors： Tanos BE,Yang HJ,Soni R,Wang WJ,Macaluso FP,Asara JM,Tsou MF

更新日期：2013-01-15 00:00:00

abstract：:To understand how chromosome shapes are determined by actions of condensins and cohesin, we devised a series of protocols in which their levels are precisely changed in Xenopus egg extracts. When the relative ratio of condensin I to II is forced to be smaller, embryonic chromosomes become shorter and thicker, being re...

journal_title：Genes & development

authors： Shintomi K,Hirano T

更新日期：2011-07-15 00:00:00

abstract：:Long noncoding RNAs (lncRNAs) can trigger repressive chromatin, but how they recruit silencing factors remains unclear. In Schizosaccharomyces pombe, heterochromatin assembly on transcribed noncoding pericentromeric repeats requires both RNAi and RNAi-independent mechanisms. In Saccharomyces cerevisiae, which lacks a ...

journal_title：Genes & development

authors： Marina DB,Shankar S,Natarajan P,Finn KJ,Madhani HD

更新日期：2013-09-01 00:00:00

abstract：:The alternatively spliced exon EIIIB is regulated in a cell type-specific manner in the rat fibronectin gene. Splicing of EIIIB into fibronectin mRNA is dependent on sequences in the intron immediately downstream of EIIIB. We show that a short, highly repeated TGCATG motif in this intron is important for cell type-spe...

journal_title：Genes & development

authors： Huh GS,Hynes RO

更新日期：1994-07-01 00:00:00

abstract：:Cell for cell, probably no human cancer is as aggressive as melanoma. It is among a handful of cancers whose dimensions are reported in millimeters. Tumor thickness approaching 4 mm presents a high risk of metastasis, and a diagnosis of metastatic melanoma carries with it an abysmal median survival of 6-9 mo. What fea...

journal_title：Genes & development

authors： Chin L,Garraway LA,Fisher DE

更新日期：2006-08-15 00:00:00

abstract：:Direct reprogramming of fibroblasts to cardiomyocytes represents a potential means of restoring cardiac function following myocardial injury. AKT1 in the presence of four cardiogenic transcription factors, GATA4, HAND2, MEF2C, and TBX5 (AGHMT), efficiently induces the cardiac gene program in mouse embryonic fibroblast...

journal_title：Genes & development

authors： Zhou H,Morales MG,Hashimoto H,Dickson ME,Song K,Ye W,Kim MS,Niederstrasser H,Wang Z,Chen B,Posner BA,Bassel-Duby R,Olson EN

更新日期：2017-09-01 00:00:00

abstract：:Staurosporine, a potent inhibitor of protein kinase C, arrests fission yeast cell elongation specifically at a stage immediately after cell division. We isolated two genes, which, when carried on multicopy plasmids, confer drug resistance in fission yeast. One, spk1+, encodes a protein kinase highly similar (54% ident...

journal_title：Genes & development

authors： Toda T,Shimanuki M,Yanagida M

更新日期：1991-01-01 00:00:00

abstract：:The presence of a single EGF receptor in Drosophila is contrasted by multiple ligands activating it. This work explores the role of two ligands, Spitz and Vein, in the embryonic ventral ectoderm. Spitz is a potent ligand, whereas Vein is an intrinsically weak activating ligand. We show that secreted Spitz emanating fr...

journal_title：Genes & development

authors： Golembo M,Yarnitzky T,Volk T,Shilo BZ

更新日期：1999-01-15 00:00:00

abstract：:Post-translational modifications of histones are significant regulators of replication, transcription, and DNA repair. Particularly, newly synthesized histone H4 in H3/H4 heterodimers becomes acetylated on N-terminal lysine residues prior to its incorporation into chromatin. Previous studies have established that the ...

journal_title：Genes & development

authors： Li Y,Zhang L,Liu T,Chai C,Fang Q,Wu H,Agudelo Garcia PA,Han Z,Zong S,Yu Y,Zhang X,Parthun MR,Chai J,Xu RM,Yang M

更新日期：2014-06-01 00:00:00

abstract：:Directed cell motility is at the basis of biological phenomena such as development, wound healing, and metastasis. It has been shown that substrate attachments mediate motility by coupling the cell's cytoskeleton with force generation. However, it has been unclear how the persistence of cell directionality is facilita...

journal_title：Genes & development

authors： Katz ZB,Wells AL,Park HY,Wu B,Shenoy SM,Singer RH

更新日期：2012-09-01 00:00:00

abstract：:Far1p is a bifunctional protein that is required to arrest the cell cycle and to establish cell polarity during yeast mating. Far1p is localized predominantly in the nucleus but accumulates in the cytoplasm in cells exposed to pheromones. Here we show that Far1p functions in both subcellular compartments: nuclear Far1...

journal_title：Genes & development

authors： Blondel M,Alepuz PM,Huang LS,Shaham S,Ammerer G,Peter M

更新日期：1999-09-01 00:00:00

abstract：:Eukaryotic genomes encode large families of deubiquitinating enzymes (DUBs). Genetic data suggest that Fat facets (Faf), a Drosophila DUB essential for patterning the compound eye, might have a novel regulatory function; Faf might reverse the ubiquitination of a specific substrate, thereby preventing proteasomal degra...

journal_title：Genes & development

authors： Chen X,Zhang B,Fischer JA

更新日期：2002-02-01 00:00:00

abstract：:Polycomb response elements (PREs) are specific cis-regulatory sequences needed for transcriptional repression of HOX and other target genes by Polycomb group (PcG) proteins. Among the many PcG proteins known in Drosophila, Pho is the only sequence-specific DNA-binding protein. To gain insight into the function of Pho,...

journal_title：Genes & development

authors： Klymenko T,Papp B,Fischle W,Köcher T,Schelder M,Fritsch C,Wild B,Wilm M,Müller J

更新日期：2006-05-01 00:00:00

abstract：:Medulloblastomas are brain tumors that arise in the cerebellum of children and contain stem cells in a perivascular niche thought to give rise to recurrence following radiation. We used several mouse models of medulloblastomas in parallel to better understand how the critical cell types in these tumors respond to ther...

journal_title：Genes & development

authors： Hambardzumyan D,Becher OJ,Rosenblum MK,Pandolfi PP,Manova-Todorova K,Holland EC

更新日期：2008-02-15 00:00:00

abstract：:A novel system to study early hematopoietic development is described. This report documents the in vitro capacity of murine embryonic stem (ES) cells to differentiate into hematopoietic precursors of most, if not all, of the colony-forming cells found in normal bone marrow. This system is used to correlate the genetic...

journal_title：Genes & development

authors： Schmitt RM,Bruyns E,Snodgrass HR

更新日期：1991-05-01 00:00:00

abstract：:SpoIIIE is an FtsK-related protein that transports the forespore chromosome across the Bacillus subtilis sporulation septum. We use membrane photobleaching and protoplast assays to demonstrate that SpoIIIE is required for septal membrane fission in the presence of trapped DNA, and that DNA is transported across separa...

journal_title：Genes & development

authors： Fleming TC,Shin JY,Lee SH,Becker E,Huang KC,Bustamante C,Pogliano K

更新日期：2010-06-01 00:00:00

abstract：:We show the intracellular localization of the Escherichia coli replication origin (oriC) and chromosome terminus during the cell division cycle by FISH. In newborn cells, oriC is localized at the old-pole-proximal nucleoid border and the terminus at the new-pole-proximal nucleoid border. One copy of replicated oriC mi...

journal_title：Genes & development

authors： Niki H,Hiraga S

更新日期：1998-04-01 00:00:00

abstract：:The product of the nuclear MRS2 gene, Mrs2p, is the only candidate splicing factor essential for all group II introns in mitochondria of the yeast Saccharomyces cerevisiae. It has been shown to be an integral protein of the inner mitochondrial membrane, structurally and functionally related to the bacterial CorA Mg(2+...

journal_title：Genes & development

authors： Gregan J,Kolisek M,Schweyen RJ

更新日期：2001-09-01 00:00:00

abstract：:The Wnt gene family encodes a group of cysteine-rich proteins implicated in intercellular signaling during several stages of vertebrate development. This family includes Wnt-1 and Wnt-3, both discovered as activated oncogenes in mouse mammary tumors. Here we describe the molecular cloning of an additional member of th...

journal_title：Genes & development

authors： Roelink H,Nusse R

更新日期：1991-03-01 00:00:00

abstract：:The choice of self-renewal versus differentiation is a fundamental issue in stem cell and cancer biology. Neural progenitors of the Drosophila post-embryonic brain, larval neuroblasts (NBs), divide asymmetrically in a stem cell-like fashion to generate a self-renewing NB and a Ganglion Mother Cell (GMC), which divides...

journal_title：Genes & development

authors： Wang H,Somers GW,Bashirullah A,Heberlein U,Yu F,Chia W

更新日期：2006-12-15 00:00:00

abstract：:Caenorhabditis elegans let-7, a founding member of the microRNA family, is predicted to bind to six sites in the 3'UTR of the mRNA of its target gene, lin-41, to down-regulate LIN-41. Here, we demonstrate that wild-type let-7 microRNA binds in vitro to RNA from the lin-41 3'UTR. This interaction is dependent on two co...

journal_title：Genes & development

authors： Vella MC,Choi EY,Lin SY,Reinert K,Slack FJ

更新日期：2004-01-15 00:00:00

abstract：:Activated Ras initiates a cascade of sequential phosphorylation events, including the protein kinases Raf, MEK, and MAP kinase. The Let-60 Ras-mediated signal transduction pathway controls vulval induction in Caenorhabditis elegans. Both Lin-45 Raf and Sur-1 MAP kinase have been determined to be essential factors duri...

journal_title：Genes & development

authors： Wu Y,Han M,Guan KL

更新日期：1995-03-15 00:00:00

abstract：:Using an in vitro system in which a 5' splice site (5'SS) RNA oligo (AAG decreases GUAAGUAdT) is capable of inducing formation of U2/U4/U5/U6 snRNP complex we show that this oligo specifically binds to U4/U5/U6 snRNP and cross-links to U6 snRNA in the absence of U2 snRNP. Moreover, 5'SS RNA oligo bound to U4/U5/U6 snR...

journal_title：Genes & development

authors： Konforti BB,Konarska MM

更新日期：1994-08-15 00:00:00

abstract：:The P family of transposable elements in Drosophila transpose by a cut-and-paste mechanism involving double-strand gap repair. We report here that a Drosophila mutagen-sensitive mutant, mus3O9, contains a mutation in IRBP (inverted repeat binding protein), the Drosophila homolog of the mammalian Ku p70 gene. We show t...

journal_title：Genes & development

authors： Beall EL,Rio DC

更新日期：1996-04-15 00:00:00

abstract：:The myogenic regulatory factor MyoD dimerizes with other positive and negative regulatory factors through a conserved region called the helix-loop-helix (HLH) domain. Using a non-DNA-binding MyoD mutant with a normal HLH domain as a dimerization competitor in gel mobility shift assays in conjunction with various MyoD ...

journal_title：Genes & development

authors： Shirakata M,Friedman FK,Wei Q,Paterson BM

更新日期：1993-12-01 00:00:00

abstract：:We isolated a novel bHLH protein gene Mesp2 (for mesoderm posterior 2) that cross-hybridizes with Mesp1 expressed in the early mouse mesoderm. Mesp2 is expressed in the rostral presomitic mesoderm, but down-regulated immediately after the formation of the segmented somites. To determine the function of MesP2 protein (...

journal_title：Genes & development

authors： Saga Y,Hata N,Koseki H,Taketo MM

更新日期：1997-07-15 00:00:00

abstract：:RNA interference (RNAi) is a flexible gene silencing mechanism that responds to double-stranded RNA by suppressing homologous genes. Here, we report the characterization of RNAi effector complexes (RISCs) that contain small interfering RNAs and microRNAs (miRNAs). We identify two putative RNA-binding proteins, the Dro...

journal_title：Genes & development

authors： Caudy AA,Myers M,Hannon GJ,Hammond SM

更新日期：2002-10-01 00:00:00

abstract：:In eubacteria, the ClpS adaptor has been proposed to be essential for degradation of N-end rule substrates by the AAA(+) protease ClpAP. To test this model, we assayed degradation of substrates bearing N-end rule sequences isolated in a genetic screen for efficient degradation tags. ClpS was not vital for degradation ...

journal_title：Genes & development

authors： Wang KH,Sauer RT,Baker TA

更新日期：2007-02-15 00:00:00