Abstract：
:The transcription factor TFIIB plays a central role in preinitiation complex assembly, providing a bridge between promoter-bound TFIID and RNA Polymerase II. TFIIB possesses sequence-specific DNA-binding ability and interacts with the TFIIB-recognition element (BRE), present in many promoters. Here we show that the BRE suppresses the basal level of transcription elicited by a core promoter, which increases the amplitude of transcriptional stimulation in the presence of an activator protein. Further, we find that an activator can disrupt the TFIIB-BRE interaction within a promoter-bound complex. Our results reveal a novel function for activators in the modulation of core promoter recognition by TFIIB.
journal_name
Genes Devjournal_title
Genes & developmentauthors
Evans R,Fairley JA,Roberts SGdoi
10.1101/gad.206901subject
Has Abstractpub_date
2001-11-15 00:00:00pages
2945-9issue
22eissn
0890-9369issn
1549-5477journal_volume
15pub_type
杂志文章abstract：:Many bacterial small regulatory RNAs (sRNAs) pair with mRNA targets, stimulating or inhibiting mRNA stability and/or translation. Regulation of these sRNAs is usually due to tight transcriptional regulation of synthesis.In this issue of Genes & Development and a related paper in Molecular Microbiology, Figueroa-Bossi ...
journal_title：Genes & development
pub_type： 评论,杂志文章
doi：10.1101/gad.1846609
更新日期：2009-09-01 00:00:00
abstract：:The Swi/Snf chromatin remodeling complex functions to alter nucleosome positions by either sliding nucleosomes on DNA or the eviction of histones. The presence of histone acetylation and activator-dependent recruitment and retention of Swi/Snf is important for its efficient function. It is not understood, however, why...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.243584.114
更新日期：2014-10-15 00:00:00
abstract：:Experiments in Xenopus have illustrated the importance of extracellular morphogens for embryonic gene regulation in vertebrates. Much less is known about how induction leads to the correct positioning of boundaries; for example, between germ layers. Here we report that the neuroectoderm/mesoderm boundary is controlled...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.409507
更新日期：2007-04-15 00:00:00
abstract：:Polycomb response elements (PREs) are specific cis-regulatory sequences needed for transcriptional repression of HOX and other target genes by Polycomb group (PcG) proteins. Among the many PcG proteins known in Drosophila, Pho is the only sequence-specific DNA-binding protein. To gain insight into the function of Pho,...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.377406
更新日期：2006-05-01 00:00:00
abstract：:Myxococcus xanthus fruiting body development is induced by amino acid limitation. The decision to grow or develop is established by the RelA-dependent stringent response and A-signaling. We identified two new members of this regulatory hierarchy, socE and the C-signaling gene csgA. SocE depletion arrests growth and in...
journal_title：Genes & development
pub_type： 杂志文章
doi：
更新日期：2000-02-15 00:00:00
abstract：:This paper examines ovarian transcription of the divergently oriented yolk protein genes 1 and 2 (yp1 and yp2) of Drosophila melanogaster. We report germ line transformation results demonstrating that yp1 and yp2 are transcribed in the same subpopulations of ovarian follicle cells. Our results show that this expressio...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.4.4.613
更新日期：1990-04-01 00:00:00
abstract：:Hepatitis B virus (HBV) infects humans and causes a wide range of clinical manifestations, from acute hepatitis to hepatocellular carcinoma (HCC). The HBV genome contains multiple promoters with gene expression regulated predominantly by the cellular transcription initiation machinery. Accordingly, the HBV-encoded pX,...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.12.8.1217
更新日期：1998-04-15 00:00:00
abstract：:Genome instability is a recurring feature of tumorigenesis. Mutation in MLL2, encoding a histone methyltransferase, is a driver in numerous different cancer types, but the mechanism is unclear. Here, we present evidence that MLL2 mutation results in genome instability. Mouse cells in which MLL2 gene deletion can be in...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.275453.115
更新日期：2016-02-15 00:00:00
abstract：:Signaling by the hormones brassinosteroid (BR) and gibberellin (GA) is critical to normal plant growth and development and is required for hypocotyl elongation in response to dark and elevated temperatures. Active BR signaling is essential for GA promotion of hypocotyl growth and suppresses the dwarf phenotype of GA m...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.243675.114
更新日期：2014-08-01 00:00:00
abstract：:Numerous lines of study have suggested that the Hox genes, encoding putative transcription factors, are key genes in the establishment of the body plan of the mammalian embryo. To examine the role of Hoxa-5 (Hox1.3) gene during development, we have used targeted mutagenesis in embryonic stem cells to produce a strain ...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.7.11.2085
更新日期：1993-11-01 00:00:00
abstract：:Persistent DNA double-strand breaks and telomeres represent genomic hazards, as they can instigate inappropriate repair reactions. Two recent papers by Oza and colleagues (pp. 912-917) and Schober and colleagues (pp. 928-938) show that both types of DNA ends are sequestered from bulk DNA by Mps3, a SUN domain protein ...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1805309
更新日期：2009-05-01 00:00:00
abstract：:Genome integrity is constantly monitored by sophisticated cellular networks, collectively termed the DNA damage response (DDR). A common feature of DDR proteins is their mobilization in response to genotoxic stress. Here, we outline how the development of various complementary methodologies has provided valuable insig...
journal_title：Genes & development
pub_type： 杂志文章,评审
doi：10.1101/gad.2021311
更新日期：2011-03-01 00:00:00
abstract：:Mutations in the CSA or CSB complementation genes cause the Cockayne syndrome, a severe genetic disorder that results in patients' death in early adulthood. CSA and CSB act in a transcription-coupled repair (TCR) pathway, but their functional relationship is not understood. We have previously shown that CSA is a subun...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.378206
更新日期：2006-06-01 00:00:00
abstract：:The sigma(28) protein is a member of the bacterial sigma(70)-family of transcription factors that directs RNA polymerase to flagellar late (class 3) promoters. The sigma(28) protein is regulated in response to flagellar assembly by the anti-sigma(28) factor FlgM. FlgM inhibits sigma(28)-dependent transcription of gene...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.380406
更新日期：2006-08-15 00:00:00
abstract：:Constitutive activation of the Wnt/APC/beta-catenin pathway is a frequent initiating event in gastrointestinal carcinogenesis. Mutations in the Adenomatous Polyposis Coli (APC) gene up-regulate Wnt signaling by stabilizing beta-catenin and causing activation of targets important in proliferation control. Here we show ...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1260605
更新日期：2005-02-01 00:00:00
abstract：:Signal transduction through the FGF receptor is essential for the specification of the vertebrate body plan. Blocking the FGF pathway in early Xenopus embryos inhibits mesoderm induction and results in truncation of the anterior-posterior axis. The Drosophila gene sprouty encodes an antagonist of FGF signaling, which ...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.191301
更新日期：2001-05-01 00:00:00
abstract：:We hypothesized that basic helix-loop-helix (bHLH) MIST1 (BHLHA15) is a "scaling factor" that universally establishes secretory morphology in cells that perform regulated secretion. Here, we show that targeted deletion of MIST1 caused dismantling of the secretory apparatus of diverse exocrine cells. Parietal cells (PC...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.285684.116
更新日期：2017-01-15 00:00:00
abstract：:A Drosophila FGF receptor homolog (DFGF-R2/DFRI) termed Heartless (Htl) is expressed in the embryonic mesoderm. The phenotypes of null mutant embryos demonstrated that Htl is a central player that is required for the development of several mesodermal lineages. No abnormalities in the primary specification of the mesod...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.10.23.2993
更新日期：1996-12-01 00:00:00
abstract：:Keratinocyte growth factor (KGF), also known as fibroblast growth factor 7 (FGF7), is synthesized by skin fibroblasts. However, its mitogenic activity is on skin keratinocytes, where it is the most potent growth factor identified thus far. To explore KGF's function in vivo, we used embryonic stem cell technology to ge...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.10.2.165
更新日期：1996-01-15 00:00:00
abstract：:The eukaryotic ribosomal RNA (rRNA) is associated cotranscriptionally with numerous factors into an enormous 90S preribosomal particle that conducts early processing of small ribosomal subunits. The assembly pathway and structure of the 90S particle is poorly understood. Here, we affinity-purified and analyzed the con...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.274688.115
更新日期：2016-03-15 00:00:00
abstract：:Myxococcus xanthus development requires CsgA, a member of the short-chain alcohol dehydrogenase (SCAD) family of proteins. We show that CsgA and SocA, a protein that can replace CsgA function in vivo, oxidize the 2'-OH glycerol moiety on cardiolipin and phosphatidylglycerol to produce diacylglycerol (DAG), dihydroxyac...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.268482.115
更新日期：2015-09-15 00:00:00
abstract：:One of the first steps in neurogenesis is the diversification of cells along the dorsoventral axis. In Drosophila the central nervous system develops from three longitudinal columns of cells: ventral cells that express the vnd/nk2 homeobox gene, intermediate cells, and dorsal cells that express the msh homeobox gene. ...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.12.22.3591
更新日期：1998-11-15 00:00:00
abstract：:MCM1 is an essential yeast DNA-binding protein that affects both minichromosome maintenance, in a manner suggesting that it has DNA replication initiation function, and gene expression. It activates alpha-specific genes together with MAT alpha 1, and represses a-specific genes together with MAT alpha 2. Alone, MCM1 ca...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.5.5.751
更新日期：1991-05-01 00:00:00
abstract：:Alu repetitive sequences are the most abundant short interspersed DNA elements in the human genome. Full-length Alu elements are composed of two tandem sequence monomers, the left and right Alu arms, both derived from the 7SL signal recognition particle RNA. Since Alu elements are common in protein-coding genes, they ...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.197467.112
更新日期：2012-09-01 00:00:00
abstract：:The timing of asexual fruiting body formation during Aspergillus nidulans colony development is precisely regulated so that conidiophores are typically produced 1-2 mm behind the growing edge of the colony. Mutations in any of four A. nidulans genes, flbB, flbC, flbD, or flbE, result in colonies that are delayed at le...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.9.4.491
更新日期：1995-02-15 00:00:00
abstract：:The N-myc gene encodes a putative transcription factor that is thought to function in the regulation of gene expression during cell differentiation and/or growth. To examine the role of N-myc during development, we have used targeted mutagenesis in embryonic stem cells to produce a mouse line that carries an N-myc nul...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.6.12a.2248
更新日期：1992-12-01 00:00:00
abstract：:Neurofibromatosis type 1 (NF1) is a common neurodevelopmental disorder caused by impaired function of the neurofibromin RAS regulator. Using a combination of Nf1 genetically engineered mice and pharmacological/genetic inhibition approaches, we report that neurofibromin differentially controls neural stem cell (NSC) pr...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.261677.115
更新日期：2015-08-15 00:00:00
abstract：:Treatment of early Xenopus embryos with retinoic acid (RA) produces a concentration-dependent series of defects in anterior axial structures that range from small deletions to embryos lacking heads. The graded series of axial defects obtained after RA administration to early embryos appears to result, in part, from ac...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.5.2.175
更新日期：1991-02-01 00:00:00
abstract：:Riboswitches are RNA elements that undergo a shift in structure in response to binding of a regulatory molecule. These elements are encoded within the transcript they regulate, and act in cis to control expression of the coding sequence(s) within that transcript; their function is therefore distinct from that of small...
journal_title：Genes & development
pub_type： 杂志文章,评审
doi：10.1101/gad.1747308
更新日期：2008-12-15 00:00:00
abstract：:The Snail zinc-finger transcription factors trigger epithelial-mesenchymal transitions (EMTs), endowing epithelial cells with migratory and invasive properties during both embryonic development and tumor progression. During EMT, Snail provokes the loss of epithelial markers, as well as changes in cell shape and the ex...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.294104
更新日期：2004-05-15 00:00:00
