Abstract：
:To investigate the relationship between splicing and polyadenylation during the production of vertebrate mRNAs, we examined the effect of mutation of a poly(A) site on splicing of upstream introns. Mutation of the AAUAAA polyadenylation consensus sequence inhibited in vitro splicing of an upstream intron. The magnitude of the depression depended on the magnesium concentration. Dependence of splicing on polyadenylation signals suggests the existence of interaction between polyadenylation and splicing factors. In multi-intron precursor RNAs containing duplicated splice sites, mutation of the poly(A) site inhibited removal of the last intron, but not the removal of introns farther upstream. Inhibition of removal of only the last intron suggests segmental recognition of multi-exon precursor RNAs and is consistent with previous suggestions that signals at both ends of an exon are required for effective splicing of an upstream intron.
journal_name
Genes Devjournal_title
Genes & developmentauthors
Niwa M,Berget SMdoi
10.1101/gad.5.11.2086subject
Has Abstractpub_date
1991-11-01 00:00:00pages
2086-95issue
11eissn
0890-9369issn
1549-5477journal_volume
5pub_type
杂志文章abstract：:MicroRNAs (miRNAs) and transcription factors (TFs) are primary metazoan gene regulators. Whereas much attention has focused on finding the targets of both miRNAs and TFs, the transcriptional networks that regulate miRNA expression remain largely unexplored. Here, we present the first genome-scale Caenorhabditis elegan...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1678608
更新日期：2008-09-15 00:00:00
abstract：:Genomic imprinting is an epigenetic phenomenon leading to parentally biased gene expression. Throughout the years, extensive efforts have been made to characterize the epigenetic marks underlying imprinting in animals and plants. As a result, DNA methylation asymmetries between parental genomes emerged as the primary ...
journal_title：Genes & development
pub_type： 杂志文章,评审
doi：10.1101/gad.332924.119
更新日期：2020-01-01 00:00:00
abstract：:The PWI motif is a highly conserved domain of unknown function in the SRm160 splicing and 3'-end cleavage-stimulatory factor, as well as in several other known or putative pre-mRNA processing components. We show here that the PWI motif is a new type of RNA/DNA-binding domain that has an equal preference for single- an...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1060403
更新日期：2003-02-15 00:00:00
abstract：:In Escherichia coli, the major nucleoid protein H-NS limits transcription by acting as a repressor or transcriptional silencer, presumably by its ability to close the looped chromosome domains in the nucleoid through DNA-protein-DNA bridging. Here, we demonstrate the direct involvement of H-NS as a positive factor sti...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.576310
更新日期：2010-07-01 00:00:00
abstract：:The HOP2 and MND1 genes are indispensable for meiotic recombination. The products of these genes associate to form a stable heterodimeric complex that binds DNA and stimulates the recombinase activity of Rad51 and Dmc1. Here we conduct molecular studies to delineate the action mechanism of the Hop2-Mnd1 complex. We pr...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1563007
更新日期：2007-07-15 00:00:00
abstract：:The recombination site dif is the target on the Escherichia coli chromosome of the site-specific recombinases XerC and XerD. The dif/XerC-D system plays a role during the cell cycle, probably by favoring sister chromosome monomerization or separation. A phenomenon of regional control over dif activity, also analyzed i...
journal_title：Genes & development
pub_type： 评论,杂志文章
doi：10.1101/gad.10.9.1152
更新日期：1996-05-01 00:00:00
abstract：:Noncoding RNA regulators have been implicated in almost all imaginable cellular processes. Here we review how regulatory small RNAs such as Spot42, SgrS, GlmY, and GlmZ and a cis-encoded ribozyme in glmS mRNA control sugar metabolism. Besides discussing the physiological implications, we show how the study of these mo...
journal_title：Genes & development
pub_type： 杂志文章,评审
doi：10.1101/gad.1717808
更新日期：2008-11-01 00:00:00
abstract：:The ultraviolet (UV)-induced (6-4) pyrimidine-pyrimidone photoproduct [(6-4) PP] confers a large structural distortion in DNA. Here we examine in human cells the roles of translesion synthesis (TLS) DNA polymerases (Pols) in promoting replication through a (6-4) TT photoproduct carried on a duplex plasmid where bidire...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1872810
更新日期：2010-01-15 00:00:00
abstract：:Thousands of eukaryotic protein-coding genes are noncanonically spliced to produce circular RNAs. Bioinformatics has indicated that long introns generally flank exons that circularize in Drosophila, but the underlying mechanisms by which these circular RNAs are generated are largely unknown. Here, using extensive muta...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.270421.115
更新日期：2015-10-15 00:00:00
abstract：:Oxidative tissues such as heart undergo a dramatic perinatal mitochondrial biogenesis to meet the high-energy demands after birth. PPARgamma coactivator-1 (PGC-1) alpha and beta have been implicated in the transcriptional control of cellular energy metabolism. Mice with combined deficiency of PGC-1alpha and PGC-1beta ...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1661708
更新日期：2008-07-15 00:00:00
abstract：:The Drosophila proteins Transformer (Tra) and Transformer2 (Tra2) regulate the sex-specific alternative splicing of Drosophila doublesex (dsx) pre-mRNA by specifically binding to a splicing enhancer (dsx repeat element; dsxRE) located 300 nucleotides (nt) downstream from a female-specific 3' splice site. In this paper...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.8.14.1703
更新日期：1994-07-15 00:00:00
abstract：:AMP-activated protein kinase (AMPK) is a sensor of energy status that maintains cellular energy homeostasis. It arose very early during eukaryotic evolution, and its ancestral role may have been in the response to starvation. Recent work shows that the kinase is activated by increases not only in AMP, but also in ADP....
journal_title：Genes & development
pub_type： 杂志文章,评审
doi：10.1101/gad.17420111
更新日期：2011-09-15 00:00:00
abstract：:Long understood as a bona fide tumor suppressor that safeguards the integrity of the genome via regulating numerous cellular outcomes, p53 may also exert its decisive and versatile functions by controlling DNA methylation. In this issue of Genes & Development, Tovy and colleagues (pp. 959-972) report that, in naïve mo...
journal_title：Genes & development
pub_type： 评论,杂志文章,评审
doi：10.1101/gad.302364.117
更新日期：2017-05-15 00:00:00
abstract：:Children with trisomy 21/Down syndrome (DS) are at high risk to develop acute megakaryoblastic leukemia (DS-AMKL) and the related transient leukemia (DS-TL). The factors on human chromosome 21 (Hsa21) that confer this predisposing effect, especially in synergy with consistently mutated transcription factor GATA1 (GATA...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1856210
更新日期：2010-03-01 00:00:00
abstract：:Abcission, the natural shedding of leaves, flowers and fruits, is a fundamental component of plant development. Abscission is a highly regulated process that occurs at distinct zones of cells that undergo enlargement and subsequent separation. Although some components of abscission, including accumulation of the hormo...
journal_title：Genes & development
pub_type： 杂志文章
doi：
更新日期：2000-01-01 00:00:00
abstract：:Cells have evolved checkpoint responses to arrest or delay the cell cycle, activate DNA repair networks, or induce apoptosis after genomic perturbation. Cells have also evolved the translesion synthesis processes to tolerate genomic lesions by either error-free or error-prone repair. Here, we show that after a replica...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1043203
更新日期：2003-01-01 00:00:00
abstract：:Programmed cell death occurs in a highly reproducible manner during Caenorhabditis elegans development. We demonstrate that, during embryogenesis, miR-35 and miR-58 bantam family microRNAs (miRNAs) cooperate to prevent the precocious death of mothers of cells programmed to die by repressing the gene egl-1, which encod...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.288555.116
更新日期：2017-01-15 00:00:00
abstract：:B-cell-specific transcription of immunoglobulin genes is mediated by the interaction of a POU domain containing transcription factor Oct-1 or Oct-2, with the B-cell-specific coactivator OCA-B (Bob-1, OBF-1) and a prototype octamer element. We find that OCA-B binds DNA directly in the major groove between the two subdo...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.10.16.2079
更新日期：1996-08-15 00:00:00
abstract：:The product of the zygotically active decapentaplegic (dpp) gene appears to function as a morphogen that specifies positional information in the dorsal half of the Drosophila embryo. The dorsal-specific transcription of dpp is the key step in establishing a morphogen gradient. We demonstrate here that multiple regions...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.7.4.694
更新日期：1993-04-01 00:00:00
abstract：:Alternative pre-mRNA splicing determines many changes in gene expression during development. Two regulators known to control splicing patterns during neuron and muscle differentiation are the polypyrimidine tract-binding protein (PTB) and its neuronal homolog nPTB. These proteins repress certain exons in early myoblas...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1500707
更新日期：2007-01-01 00:00:00
abstract：:DOCK180 is involved in integrin signaling through CrkII-p130(Cas) complexes. We have studied the involvement of DOCK180 in Rac1 signaling cascades. DOCK180 activated JNK in a manner dependent on Rac1, Cdc42Hs, and SEK, and overexpression of DOCK180 increased the amount of GTP-bound Rac1 in 293T cells. Coexpression of ...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.12.21.3331
更新日期：1998-11-01 00:00:00
abstract：:Disruptions in the use of skeletal muscle lead to muscle atrophy. After short periods of disuse, muscle atrophy is reversible, and even after prolonged periods of inactivity, myofiber degeneration is uncommon. The pathways that regulate atrophy, initiated either by peripheral nerve damage, immobilization, aging, catab...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1318305
更新日期：2005-07-15 00:00:00
abstract：:Cyclin D1 is part of a cell cycle control node consistently deregulated in most human cancers. However, studies with cyclin D1-null mice indicate that it is dispensable for normal mouse development as well as cell growth in culture. Here, we provide evidence that ras-mediated tumorigenesis depends on signaling pathway...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.12.16.2469
更新日期：1998-08-15 00:00:00
abstract：:The homeless (hls) gene of Drosophila is required for anteroposterior and dorsoventral axis formation during oogenesis. At a low frequency, females homozygous for mutations in hls generate early egg chambers in which the oocyte is positioned incorrectly within the cyst. At a high frequency, late-stage egg chambers exh...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.9.20.2495
更新日期：1995-10-15 00:00:00
abstract：:An RNA-based gene silencing pathway that protects bacteria and archaea from viruses and other genome invaders is hypothesized to arise from guide RNAs encoded by CRISPR loci and proteins encoded by the cas genes. CRISPR loci contain multiple short invader-derived sequences separated by short repeats. The presence of v...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1742908
更新日期：2008-12-15 00:00:00
abstract：:Accurate completion of replication relies on the ability of cells to activate error-free recombination-mediated DNA damage bypass at sites of perturbed replication. However, as anti-recombinase activities are also recruited to replication forks, how recombination-mediated damage bypass is enabled at replication stress...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.265629.115
更新日期：2015-10-01 00:00:00
abstract：:P-element transformants of a single rRNA gene (rDNA) were used to investigate the relationship between the organization of the nucleolus organizer (NO) and rDNA function in Drosophila melanogaster. In situ hybridization to rRNA in polytene nuclei of salivary glands demonstrated that an rRNA gene can be transcribed at ...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.2.12b.1745
更新日期：1988-12-01 00:00:00
abstract：:Activating JAK2 point mutations are implicated in the pathogenesis of myeloid and lymphoid malignancies, including high-risk B-cell acute lymphoblastic leukemia (B-ALL). In preclinical studies, treatment of JAK2 mutant leukemias with type I JAK2 inhibitors (e.g., Food and Drug Administration [FDA]-approved ruxolitinib...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.307504.117
更新日期：2018-06-01 00:00:00
abstract：:RNA polymerase II (Pol II) transcribes all protein-coding genes and many noncoding RNAs in eukaryotic genomes. Although Pol II is a complex, 12-subunit enzyme, it lacks the ability to initiate transcription and cannot consistently transcribe through long DNA sequences. To execute these essential functions, an array of...
journal_title：Genes & development
pub_type： 杂志文章,评审
doi：10.1101/gad.335679.119
更新日期：2020-04-01 00:00:00
abstract：:Chromatin is organized into higher-order structures that form subcompartments in interphase nuclei. Different categories of specialized enzymes act on chromatin and regulate its compaction and biophysical characteristics in response to physiological conditions. We present an overview of the function of chromatin struc...
journal_title：Genes & development
pub_type： 杂志文章,评审
doi：10.1101/gad.307702.117
更新日期：2017-11-15 00:00:00
