Cas6 is an endoribonuclease that generates guide RNAs for invader defense in prokaryotes.

abstract：:The transcription cofactor MAL is regulated by free actin levels and thus by actin dynamics. MAL, together with its DNA-binding partner, SRF, is required for invasive cell migration and in experimental metastasis. Although MAL/SRF has many targets, we provide genetic evidence in both Drosophila and human cellular mode...

journal_title：Genes & development

authors： Salvany L,Muller J,Guccione E,Rørth P

更新日期：2014-05-15 00:00:00

abstract：:Mammalian cells possess two amino acid-sensing kinases: general control nonderepressible 2 (GCN2) and mechanistic target of rapamycin complex 1 (mTORC1). Their combined effects orchestrate cellular adaptation to amino acid levels, but how their activities are coordinated remains poorly understood. Here, we demonstrate...

journal_title：Genes & development

authors： Ye J,Palm W,Peng M,King B,Lindsten T,Li MO,Koumenis C,Thompson CB

更新日期：2015-11-15 00:00:00

abstract：:A recent study has shown that the dimeric form of the 50-kD DNA-binding subunit of the NF-kappa B transcription factor is commonly associated with two molecules of a 65-kD protein (p65) and that p65 appears to be required for inactivation of the p50 dimer by the inhibitory subunit I kappa B. Here, we provide evidence ...

journal_title：Genes & development

authors： Urban MB,Baeuerle PA

更新日期：1990-11-01 00:00:00

abstract：:The orphan nuclear receptor TLX regulates neural stem cell self-renewal in the adult brain and functions primarily as a transcription repressor through recruitment of Atrophin corepressors, which bind to TLX via a conserved peptide motif termed the Atro box. Here we report crystal structures of the human and insect TL...

journal_title：Genes & development

authors： Zhi X,Zhou XE,He Y,Searose-Xu K,Zhang CL,Tsai CC,Melcher K,Xu HE

更新日期：2015-02-15 00:00:00

abstract：:The human T cell-specific transcription factor TCF-1 alpha plays a key role in the tissue-specific activation of the T cell receptor (TCR) C alpha enhancer and binds to pyrimidine-rich elements (5'-PyCTTTG-3') present in a variety of other T cell-specific control regions. Using amino acid sequence information derived ...

journal_title：Genes & development

authors： Waterman ML,Fischer WH,Jones KA

更新日期：1991-04-01 00:00:00

abstract：:Cytosolic RNA/DNA sensing elicits primary defense against viral pathogens. Interferon regulatory factor 3 (IRF3), a key signal mediator/transcriptional factor of the antiviral-sensing pathway, is indispensible for interferon production and antiviral defense. However, how the status of IRF3 activation is controlled rem...

journal_title：Genes & development

authors： Meng F,Zhou R,Wu S,Zhang Q,Jin Q,Zhou Y,Plouffe SW,Liu S,Song H,Xia Z,Zhao B,Ye S,Feng XH,Guan KL,Zou J,Xu P

更新日期：2016-05-01 00:00:00

abstract：:The Drosophila proteins Transformer (Tra) and Transformer2 (Tra2) regulate the sex-specific alternative splicing of Drosophila doublesex (dsx) pre-mRNA by specifically binding to a splicing enhancer (dsx repeat element; dsxRE) located 300 nucleotides (nt) downstream from a female-specific 3' splice site. In this paper...

journal_title：Genes & development

authors： Tian M,Maniatis T

更新日期：1994-07-15 00:00:00

abstract：:Transcription of erythroid-expressed genes and normal erythroid development in vivo are dependent on a regulatory protein (GATA-1) that recognizes a consensus GATA motif. GATA-1 expression is itself restricted to erythroid progenitors and to two related hematopoietic lineages, megakaryocytes and mast cells. During cel...

journal_title：Genes & development

authors： Tsai SF,Strauss E,Orkin SH

更新日期：1991-06-01 00:00:00

abstract：:Fgf-10-deficient mice (Fgf-10(-/-)) were generated to determine the role(s) of Fgf-10 in vertebrate development. Limb bud initiation was abolished in Fgf-10(-/-) mice. Strikingly, Fgf-10(-/-) fetuses continued to develop until birth, despite the complete absence of both fore- and hindlimbs. Fgf-10 is necessary for api...

journal_title：Genes & development

authors： Min H,Danilenko DM,Scully SA,Bolon B,Ring BD,Tarpley JE,DeRose M,Simonet WS

更新日期：1998-10-15 00:00:00

abstract：:Alternative pre-mRNA splicing determines many changes in gene expression during development. Two regulators known to control splicing patterns during neuron and muscle differentiation are the polypyrimidine tract-binding protein (PTB) and its neuronal homolog nPTB. These proteins repress certain exons in early myoblas...

journal_title：Genes & development

authors： Boutz PL,Chawla G,Stoilov P,Black DL

更新日期：2007-01-01 00:00:00

abstract：:We demonstrate here that the previously described bacterial promoter upstream element (UP element) consists of two distinct subsites, each of which, by itself, can bind the RNA polymerase holoenzyme alpha subunit carboxy-terminal domain (RNAP alphaCTD) and stimulate transcription. Using binding-site-selection experime...

journal_title：Genes & development

authors： Estrem ST,Ross W,Gaal T,Chen ZW,Niu W,Ebright RH,Gourse RL

更新日期：1999-08-15 00:00:00

abstract：:The Neurospora crassa clock-controlled gene (ccg-2) is transcriptionally activated by the circadian clock in a time-of-day-specific manner. Transcript and sequence analyses of ccg-2 reveal that the predicted Ccg-2 polypeptide bears significant similarity to a class of low-molecular-weight, cysteine-rich, hydrophobic p...

journal_title：Genes & development

authors： Bell-Pedersen D,Dunlap JC,Loros JJ

更新日期：1992-12-01 00:00:00

abstract：:Chromatin structure is believed to be important for a number of cellular processes, including transcription. However, the role of nucleosomes in transcription is not well understood. We have identified the yeast histone locus HTB1-HTB1, encoding histones H2A and H2B, as a suppressor of solo delta insertion mutations t...

journal_title：Genes & development

authors： Clark-Adams CD,Norris D,Osley MA,Fassler JS,Winston F

更新日期：1988-02-01 00:00:00

abstract：:Transcription factors represent one of the largest groups of proteins regulated by SUMO (small ubiquitin-like modifier) modification, and their sumoylation is usually associated with transcriptional repression. To investigate whether sumoylation plays a general role in regulating transcription in yeast, we determined ...

journal_title：Genes & development

authors： Rosonina E,Duncan SM,Manley JL

更新日期：2010-06-15 00:00:00

abstract：:The RAG-1 protein plays an essential role in V(D)j recombination, but its exact function has not yet been defined. Here we report that a particular mutation in RAG-1 affects recombination by altering the specificity of target sequence usage. Recombination mediated by wild-type RAG-1 is tolerant of a wide range of codi...

journal_title：Genes & development

authors： Sadofsky MJ,Hesse JE,van Gent DC,Gellert M

更新日期：1995-09-01 00:00:00

abstract：:Although branchpoint recognition is an essential component of intron excision during the RNA splicing process, the branchpoint itself is frequently assumed to be a basal, rather than regulatory, sequence feature. However, this assumption has not been systematically tested due to the technical difficulty of identifying...

journal_title：Genes & development

authors： Pineda JMB,Bradley RK

更新日期：2018-04-01 00:00:00

abstract：:The association of proteins with the branch site region during pre-mRNA splicing was probed using a novel methodology to site-specifically modify the pre-mRNA with the photo-reagent benzophenone. Three sets of proteins were distinguished by the kinetics of their associations with pre-mRNAs, by their association with d...

journal_title：Genes & development

authors： MacMillan AM,Query CC,Allerson CR,Chen S,Verdine GL,Sharp PA

更新日期：1994-12-15 00:00:00

abstract：:The regulation of gene transcription is critical for the proper development and growth of an organism. The transcription of protein-coding genes initiates at the RNA polymerase II core promoter, which is a diverse module that can be controlled by many different elements such as the TATA box and downstream core promote...

journal_title：Genes & development

authors： Juven-Gershon T,Hsu JY,Kadonaga JT

更新日期：2008-10-15 00:00:00

abstract：:Our understanding of eukaryotic transcriptional activation mechanisms has been hampered by an inability to identify the direct in vivo targets of activator proteins, primarily because of lack of appropriate experimental methods. To circumvent this problem, we have developed a fluorescence resonance energy transfer (FR...

journal_title：Genes & development

authors： Bhaumik SR,Raha T,Aiello DP,Green MR

更新日期：2004-02-01 00:00:00

abstract：:To investigate the importance of sequences between the yeast (Saccharomyces cerevisiae) branch point (TACTAAC box) and 3' splice site (AG), we generated a series of pre-mRNA substrates that differed in the length of RNA retained on the 3' side of the TACTAAC box. These pre-mRNAs were compared as substrates for the fir...

journal_title：Genes & development

authors： Rymond BC,Torrey DD,Rosbash M

更新日期：1987-05-01 00:00:00

abstract：:Janus kinase 2 (JAK2) is a central kinase in hematopoietic stem/progenitor cells (HSPCs), and its uncontrolled activation is a prominent oncogenic driver of hematopoietic neoplasms. However, molecular mechanisms underlying the regulation of JAK2 have remained elusive. Here we report that the Casitas B-cell lymphoma (C...

journal_title：Genes & development

authors： Lv K,Jiang J,Donaghy R,Riling CR,Cheng Y,Chandra V,Rozenova K,An W,Mohapatra BC,Goetz BT,Pillai V,Han X,Todd EA,Jeschke GR,Langdon WY,Kumar S,Hexner EO,Band H,Tong W

更新日期：2017-05-15 00:00:00

abstract：:Human ELAV proteins are implicated in cell growth and differentiation via regulation of mRNA expression in the cytoplasm. In human embryonic teratocarcinoma (hNT2) cells transfected with the human neuronal ELAV-like protein, Hel-N1, neurites formed, yet cells were not terminally differentiated. Cells in which neurite ...

journal_title：Genes & development

authors： Antic D,Lu N,Keene JD

更新日期：1999-02-15 00:00:00

abstract：:Mice mutant for the bHLH gene Hes1, which is known to keep cells in a proliferative state, mostly lack thymus. Transfer of Hes1-null fetal liver cells into RAG2-null host mice normally reconstitutes B cells but fails to generate mature T cells in the thymus. In the reconstituted thymus, T cell differentiation is arres...

journal_title：Genes & development

authors： Tomita K,Hattori M,Nakamura E,Nakanishi S,Minato N,Kageyama R

更新日期：1999-05-01 00:00:00

abstract：:The mechanisms by which homeoproteins bind selectively to target genes in vivo have long remained unresolved. Here we report that PIAS1 confers DNA-binding specificity on the Msx1 homeoprotein by regulating its subnuclear localization and proximity to target genes. We demonstrate that the interaction of Msx1 with PIAS...

journal_title：Genes & development

authors： Lee H,Quinn JC,Prasanth KV,Swiss VA,Economides KD,Camacho MM,Spector DL,Abate-Shen C

更新日期：2006-04-01 00:00:00

abstract：:Export to the cytoplasm of U3 RNA transcribed from a rat U3 gene injected into the nucleus of Xenopus oocytes indicates that the biogenesis of U3 RNA, like that of the previously studied Sm-precipitable nucleoplasmic snRNAs (U1, U2, U4, and U5), includes a cytoplasmic phase. The regulation of import of the U3 snRNA in...

journal_title：Genes & development

authors： Baserga SJ,Gilmore-Hebert M,Yang XW

更新日期：1992-06-01 00:00:00

abstract：:Interruption of translation in Escherichia coli can lead to the addition of an 11-residue carboxy-terminal peptide tail to the nascent chain. This modification is mediated by SsrA RNA (also called 10Sa RNA and tmRNA) and marks the tagged polypeptide for proteolysis. Degradation in vivo of lambda repressor amino-termin...

journal_title：Genes & development

authors： Gottesman S,Roche E,Zhou Y,Sauer RT

更新日期：1998-05-01 00:00:00

abstract：:The recombination site dif is the target on the Escherichia coli chromosome of the site-specific recombinases XerC and XerD. The dif/XerC-D system plays a role during the cell cycle, probably by favoring sister chromosome monomerization or separation. A phenomenon of regional control over dif activity, also analyzed i...

journal_title：Genes & development

authors： Cornet F,Louarn J,Patte J,Louarn JM

更新日期：1996-05-01 00:00:00

abstract：:Adenomatous polyposis coli (APC) regulates the activity of β-catenin, an integral component of Wnt signaling. However, the selective role of the APC-β-catenin pathway in cerebral cortical development is unknown. Here we genetically dissected the relative contributions of APC-regulated β-catenin signaling in cortical p...

journal_title：Genes & development

authors： Nakagawa N,Li J,Yabuno-Nakagawa K,Eom TY,Cowles M,Mapp T,Taylor R,Anton ES

更新日期：2017-08-15 00:00:00

abstract：:Using an in vitro system in which a 5' splice site (5'SS) RNA oligo (AAG decreases GUAAGUAdT) is capable of inducing formation of U2/U4/U5/U6 snRNP complex we show that this oligo specifically binds to U4/U5/U6 snRNP and cross-links to U6 snRNA in the absence of U2 snRNP. Moreover, 5'SS RNA oligo bound to U4/U5/U6 snR...

journal_title：Genes & development

authors： Konforti BB,Konarska MM

更新日期：1994-08-15 00:00:00

abstract：:The assembly of activated RNA polymerase II (pol II) transcription complexes has been investigated by assaying whether pre-assembly of intermediate complexes reduces the extended time required for start-site melting. The results show that a closed complex requiring factors IIA, IID, and the acidic activator GAL4-AH fo...

journal_title：Genes & development

authors： Wang W,Gralla JD,Carey M

更新日期：1992-09-01 00:00:00