Abstract:
:In Escherichia coli, the major nucleoid protein H-NS limits transcription by acting as a repressor or transcriptional silencer, presumably by its ability to close the looped chromosome domains in the nucleoid through DNA-protein-DNA bridging. Here, we demonstrate the direct involvement of H-NS as a positive factor stimulating translation of the malT mRNA. In vitro studies showed that H-NS facilitates a repositioning of the 30S preinitiation complex on the malT mRNA. H-NS stimulation of translation depended on the AU-rich -35 to -40 region of the mRNA. Several additional examples were found demonstrating a novel function for H-NS in translation of genes with suboptimal ribosome-binding sequences.
journal_name
Genes Devjournal_title
Genes & developmentauthors
Park HS,Ostberg Y,Johansson J,Wagner EG,Uhlin BEdoi
10.1101/gad.576310subject
Has Abstractpub_date
2010-07-01 00:00:00pages
1345-50issue
13eissn
0890-9369issn
1549-5477pii
24/13/1345journal_volume
24pub_type
杂志文章abstract::Ubiquitin-mediated proteolysis controls the abundance of many cell cycle regulatory proteins. Recent work in Saccharomyces cerevisiae suggests that a complex consisting of Cdc53, Skp1, and a third component known as an F-box protein (termed SCF) in combination with Cdc34 specifically targets regulatory proteins for de...
journal_title:Genes & development
pub_type: 杂志文章
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journal_title:Genes & development
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journal_title:Genes & development
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journal_title:Genes & development
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更新日期:2011-04-01 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
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abstract::NF-E1, a DNA-binding protein that recognizes the general consensus motif WGATAR, is the first tissue-specific factor to be identified in erythroid cells. Using a probe from the murine GF-1 (NF-E1) cDNA clone, we isolated three homologous chicken cDNAs: One of these corresponds to an mRNA (NF-E1a) that is abundantly an...
journal_title:Genes & development
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journal_title:Genes & development
pub_type: 杂志文章
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更新日期:2005-01-15 00:00:00
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journal_title:Genes & development
pub_type: 评论,杂志文章
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abstract::SET8 (also known as PR-SET7) is a histone H4-Lys-20-specific methyltransferase that is implicated in cell-cycle-dependent transcriptional silencing and mitotic regulation in metazoans. Herein we report the crystal structure of human SET8 (hSET8) bound to a histone H4 peptide bearing Lys-20 and the product cofactor S-a...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.1318405
更新日期:2005-06-15 00:00:00
abstract::Polycomb response elements (PREs) are specific cis-regulatory sequences needed for transcriptional repression of HOX and other target genes by Polycomb group (PcG) proteins. Among the many PcG proteins known in Drosophila, Pho is the only sequence-specific DNA-binding protein. To gain insight into the function of Pho,...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.377406
更新日期:2006-05-01 00:00:00
abstract::A complex array of genetic factors regulates neuronal dendrite morphology. Epigenetic regulation of gene expression represents a plausible mechanism to control pathways responsible for specific dendritic arbor shapes. By studying the Drosophila dendritic arborization (da) neurons, we discovered a role of the double-br...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.239962.114
更新日期:2014-09-01 00:00:00
abstract::Transabdominal (Tab), a dominant mutation in the Bithorax Complex (BX-C) of Drosophila, creates a sexually dimorphic pattern of segmental transformation that has complete penetrance and expressivity. Specific regions within the notum of the second thoracic segment (T2) are transformed into abdominal-like cuticle; thus...
journal_title:Genes & development
pub_type: 杂志文章
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更新日期:1987-04-01 00:00:00
abstract::Outward migration of epidermal progenitors occurs with induction of hundreds of differentiation genes, but the identities of all regulators required for this process are unknown. We used laser capture microdissection followed by RNA sequencing to identify calmodulin-like 5 (CALML5) as the most enriched gene in differe...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.267708.115
更新日期:2015-11-01 00:00:00
abstract::Post-translational processes are essential for the generation and dynamics of mammalian circadian rhythms. In particular, phosphorylation of the key circadian protein PER2 precisely controls the period and phase of circadian oscillations. However, the mechanisms underlying that control are poorly understood. Here, we ...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.512209
更新日期:2009-03-15 00:00:00
abstract::DinI is a recently described negative regulator of the SOS response in Escherichia coli. Here we show that it physically interacts with RecA and prevents the binding of single-stranded DNA to RecA, which is required for the activation of the latter. DinI also displaces ssDNA from a stable RecA-DNA cofilament, thus eli...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.862901
更新日期:2001-02-15 00:00:00
abstract::This year is the centenary of Edward G. Conklin's signal findings in embryology: the elucidation of complete cell lineages and the discovery of localized maternal determinants. Conklin used ascidian embryos to elucidate universal principles in embryology. A century later, ascidians, or sea squirts, have not only enter...
journal_title:Genes & development
pub_type: 杂志文章,评审
doi:10.1101/gad.1365805
更新日期:2005-10-15 00:00:00
abstract::Ectopic repression of retinoic acid (RA) receptor target genes by PML/RARA and PLZF/RARA fusion proteins through aberrant recruitment of nuclear corepressor complexes drives cellular transformation and acute promyelocytic leukemia (APL) development. In the case of PML/RARA, this repression can be reversed through trea...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.512009
更新日期:2009-05-15 00:00:00
abstract::Cell cycle progression is dependent on two major waves of gene expression. Early cell cycle gene expression occurs during G1/S to generate factors required for DNA replication, while late cell cycle gene expression begins during G2 to prepare for mitosis. Here we demonstrate that the MuvB complex-comprised of LIN9, LI...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.181933.111
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journal_title:Genes & development
pub_type: 杂志文章
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更新日期:2014-12-15 00:00:00
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journal_title:Genes & development
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journal_title:Genes & development
pub_type: 杂志文章
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更新日期:1994-04-15 00:00:00
abstract::In enteric bacteria, the transcription factor σ(E) maintains membrane homeostasis by inducing synthesis of proteins involved in membrane repair and two small regulatory RNAs (sRNAs) that down-regulate synthesis of abundant membrane porins. Here, we describe the discovery of a third σ(E)-dependent sRNA, MicL (mRNA-inte...
journal_title:Genes & development
pub_type: 杂志文章
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更新日期:2014-07-15 00:00:00
abstract::Autophagy is crucial for cellular homeostasis and plays important roles in tumorigenesis. FIP200 (FAK family-interacting protein of 200 kDa) is an essential autophagy gene required for autophagy induction, functioning in the ULK1-ATG13-FIP200 complex. Our previous studies showed that conditional knockout of FIP200 sig...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.237354.113
更新日期:2014-06-01 00:00:00
abstract::During development of the somatic macronucleus of Tetrahymena thermophila, the rDNA is excised from its germ-line chromosome, rearranged into a palindrome, and amplified to 10(4) copies. We have identified a cis-acting germ-line mutation, rmm11/6, that prevents amplification of the rDNA in all but approximately 1 in 1...
journal_title:Genes & development
pub_type: 杂志文章
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更新日期:1994-01-01 00:00:00
abstract::Myxococcus xanthus development requires CsgA, a member of the short-chain alcohol dehydrogenase (SCAD) family of proteins. We show that CsgA and SocA, a protein that can replace CsgA function in vivo, oxidize the 2'-OH glycerol moiety on cardiolipin and phosphatidylglycerol to produce diacylglycerol (DAG), dihydroxyac...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.268482.115
更新日期:2015-09-15 00:00:00
abstract::Hypoxia induces rapid and dramatic changes in cellular metabolism, in part through inhibition of target of rapamycin (TOR) kinase complex 1 (TORC1) activity. Genetic studies have shown the tuberous sclerosis tumor suppressors TSC1/2 and the REDD1 protein to be essential for hypoxia regulation of TORC1 activity in Dros...
journal_title:Genes & development
pub_type: 杂志文章
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abstract::Chromatin-modifying enzymes are known to be critical components for the correct differentiation of embryonic stem cells into specific lineages, such as neurons. Recently, the role of Polycomb group proteins has been studied in the specification and differentiation of muscle stem cells. In this perspective, we review a...
journal_title:Genes & development
pub_type: 评论,杂志文章
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更新日期:2011-05-15 00:00:00
abstract::Genomic imprinting is an epigenetic mechanism by which genes are expressed in a parental origin-dependent manner. We recently discovered that, like DNA methylation, oocyte-inherited H3K27me3 can also serve as an imprinting mark in mouse preimplantation embryos. In this study, we found H3K27me3 is strongly biased towar...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.323105.118
更新日期:2019-04-01 00:00:00
abstract::Intracellular heme levels must be tightly regulated to maintain proper mitochondrial respiration while minimizing toxicity, but the homeostatic mechanisms are not well understood. Here we report a novel negative feedback mechanism whereby the nuclear heme receptor Rev-erbalpha tightly controls the level of its own lig...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.1825809
更新日期:2009-09-15 00:00:00