Novel role for a bacterial nucleoid protein in translation of mRNAs with suboptimal ribosome-binding sites.

Abstract:

:In Escherichia coli, the major nucleoid protein H-NS limits transcription by acting as a repressor or transcriptional silencer, presumably by its ability to close the looped chromosome domains in the nucleoid through DNA-protein-DNA bridging. Here, we demonstrate the direct involvement of H-NS as a positive factor stimulating translation of the malT mRNA. In vitro studies showed that H-NS facilitates a repositioning of the 30S preinitiation complex on the malT mRNA. H-NS stimulation of translation depended on the AU-rich -35 to -40 region of the mRNA. Several additional examples were found demonstrating a novel function for H-NS in translation of genes with suboptimal ribosome-binding sequences.

journal_name

Genes Dev

journal_title

Genes & development

authors

Park HS,Ostberg Y,Johansson J,Wagner EG,Uhlin BE

doi

10.1101/gad.576310

subject

Has Abstract

pub_date

2010-07-01 00:00:00

pages

1345-50

issue

13

eissn

0890-9369

issn

1549-5477

pii

24/13/1345

journal_volume

24

pub_type

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