Locus-specific variation in phosphorylation state of RNA polymerase II in vivo: correlations with gene activity and transcript processing.

abstract：:The circadian rhythm of pineal melatonin requires the nocturnal increment of serotonin N-acetyltransferase (arylalkylamine N-acetyltransferase [AANAT]) protein. To date, only limited information is available in the critical issue of how AANAT protein expression is up-regulated exclusively at night regardless of its sp...

journal_title：Genes & development

authors： Kim TD,Woo KC,Cho S,Ha DC,Jang SK,Kim KT

更新日期：2007-04-01 00:00:00

abstract：:Pax3 is a key transcription factor implicated in development and human disease. To dissect the role of Pax3 in myogenesis and establish whether it is a repressor or activator, we generated loss- and gain-of-function alleles by targeting an nLacZ reporter and a sequence encoding the oncogenic fusion protein PAX3-FKHR i...

journal_title：Genes & development

authors： Relaix F,Polimeni M,Rocancourt D,Ponzetto C,Schäfer BW,Buckingham M

更新日期：2003-12-01 00:00:00

abstract：:Mismatch repair (MMR) has important roles in meiotic and mitotic recombination, DNA damage signaling, and various aspects of DNA metabolism including class-switch recombination, somatic hypermutation, and triplet-repeat expansion. Defects in MMR are responsible for human cancers characterized by microsatellite instabi...

journal_title：Genes & development

authors： Siegl-Cachedenier I,Muñoz P,Flores JM,Klatt P,Blasco MA

更新日期：2007-09-01 00:00:00

abstract：:Stomata are specialized cellular structures in the epidermis of aerial plant organs that control gas exchange (H(2)O release and CO(2) uptake) between leaves and the atmosphere by modulating the aperture of a pore flanked by two guard cells. Stomata are nonrandomly distributed, and their density is controlled by endog...

journal_title：Genes & development

authors： Berger D,Altmann T

更新日期：2000-05-01 00:00:00

abstract：:We have investigated the DNA-binding, oligomerization, and trans-activation functions of isolated segments of murine p53. We find that p53 has two autonomous DNA-binding regions. One domain, from amino acid 280 to 390, forms stable tetramers and binds DNA nonspecifically. The biological significance, if any, of this D...

journal_title：Genes & development

authors： Wang Y,Reed M,Wang P,Stenger JE,Mayr G,Anderson ME,Schwedes JF,Tegtmeyer P

更新日期：1993-12-01 00:00:00

abstract：:We describe the developmental effects of the expression of a normal and mutated Drosophila ras gene ras2 in transgenic Drosophila. A Gly14----Val14 mutant (Gly14 in ras2 is equivalent to Gly12 in mammalian ras proteins) was constructed in ras2 by site-directed mutagenesis. Inducible constructs of ras2 and ras2Val14 we...

journal_title：Genes & development

authors： Bishop JG 3rd,Corces VG

更新日期：1988-05-01 00:00:00

abstract：:A core promoter element called an initiator (Inr) overlaps the transcription start site of numerous mammalian protein-coding genes. In promoters that lack a TATA box, the Inr is functionally analogous to TATA, in that it is capable of directing basal transcription by RNA polymerase II and of determining the precise si...

journal_title：Genes & development

authors： Kaufmann J,Smale ST

更新日期：1994-04-01 00:00:00

abstract：:It is now well appreciated that the apoptosome, which governs caspase-dependent cell death, also drives nonapoptotic caspase activation to remodel cells. However, the determinants that specify whether the apoptosome acts to kill or remodel have yet to be identified. Here we report that Tango7 collaborates with the Dro...

journal_title：Genes & development

authors： D'Brot A,Chen P,Vaishnav M,Yuan S,Akey CW,Abrams JM

更新日期：2013-08-01 00:00:00

abstract：:The epidemic of obesity and diabetes is causing an increased incidence of dyslipidemia-related heart failure. While the primary etiology of lipotoxic cardiomyopathy is an elevation of lipid levels resulting from an imbalance in energy availability and expenditure, increasing evidence suggests a relationship between dy...

journal_title：Genes & development

authors： Lim HY,Wang W,Wessells RJ,Ocorr K,Bodmer R

更新日期：2011-01-15 00:00:00

abstract：:Genomic imprinting, an inherently epigenetic phenomenon defined by parent of origin-dependent gene expression, is observed in mammals and flowering plants. Genome-scale surveys of imprinted expression and the underlying differential epigenetic marks have led to the discovery of hundreds of imprinted plant genes and co...

journal_title：Genes & development

authors： Rodrigues JA,Zilberman D

更新日期：2015-12-15 00:00:00

abstract：:Previous studies have shown that differentiation of 3T3-L1 preadipocytes leads to the transcriptional activation of a group of adipose-specific genes. As an approach to defining the mechanism responsible for activating the expression of these genes, we investigated the binding of nuclear factors to the promoters of tw...

journal_title：Genes & development

authors： Christy RJ,Yang VW,Ntambi JM,Geiman DE,Landschulz WH,Friedman AD,Nakabeppu Y,Kelly TJ,Lane MD

更新日期：1989-09-01 00:00:00

abstract：:Rb mutants exhibit aneuploidy and aberrant chromosome structure during mitosis. In this issue of Genes & Development, a new paper from Longworth and colleagues (1011-1024) describes both physical and functional interactions between Drosophila Rbf1 and the dCAP-D3 subunit of condensin II. This work directly implicates ...

journal_title：Genes & development

authors： Lavoie BD

更新日期：2008-04-15 00:00:00

abstract：:Telomeres are specialized nucleoprotein structures that protect chromosome ends from DNA damage response (DDR) and DNA rearrangements. The telomeric shelterin protein TRF2 suppresses the DDR, and this function has been attributed to its abilities to trigger t-loop formation or prevent massive decompaction and loss of ...

journal_title：Genes & development

authors： Vancevska A,Douglass KM,Pfeiffer V,Manley S,Lingner J

更新日期：2017-03-15 00:00:00

abstract：:Structural cell-surface and extracellular-matrix proteins modulate intercellular signaling events during development, but how this is achieved remains largely unknown. Here we identify a novel family of Drosophila proteins, Nasrat and Polehole, that coat the oocyte surface and play two roles: They mediate assembly of ...

journal_title：Genes & development

authors： Jiménez G,González-Reyes A,Casanova J

更新日期：2002-04-15 00:00:00

abstract：:Sox2 is expressed in basal epithelial cells of the tongue, with high levels in taste bud placodes, fungiform papillae, and mature taste cells, and low levels in filiform papillae. High Sox2 expression appears to lie downstream from canonical Wnt signaling. In hypomorphic Sox2(EGFP/LP) embryos, placodes form but no mat...

journal_title：Genes & development

authors： Okubo T,Pevny LH,Hogan BL

更新日期：2006-10-01 00:00:00

abstract：:Calcineurin, a Ca2+/calmodulin dependent protein phosphatase, regulates Ca2+-dependent processes in a wide variety of cells. In the yeast, Saccharomyces cerevisiae, calcineurin effects Ca2+-dependent changes in gene expression through regulation of the Crz1p transcription factor. We show here that calcineurin dephosph...

journal_title：Genes & development

authors： Stathopoulos-Gerontides A,Guo JJ,Cyert MS

更新日期：1999-04-01 00:00:00

abstract：:The nuclear receptor Rev-erbα regulates circadian rhythm and metabolism, but its effects are modest and it has been considered to be a secondary regulator of the cell-autonomous clock. Here we report that depletion of Rev-erbα together with closely related Rev-erbβ has dramatic effects on the cell-autonomous clock as ...

journal_title：Genes & development

authors： Bugge A,Feng D,Everett LJ,Briggs ER,Mullican SE,Wang F,Jager J,Lazar MA

更新日期：2012-04-01 00:00:00

abstract：:The incretin hormone GLP1 promotes islet-cell survival via the second messenger cAMP. Here we show that mice deficient in the activity of CREB, caused by expression of a dominant-negative A-CREB transgene in pancreatic beta-cells, develop diabetes secondary to beta-cell apoptosis. Remarkably, A-CREB severely disrupted...

journal_title：Genes & development

authors： Jhala US,Canettieri G,Screaton RA,Kulkarni RN,Krajewski S,Reed J,Walker J,Lin X,White M,Montminy M

更新日期：2003-07-01 00:00:00

abstract：:Faithful inheritance of genetic information requires that DNA be copied only once each cell cycle. Initiation of DNA replication involves the establishment of a prereplication complex (pre-RC) and subsequent activation by CDK/cyclins, converting the pre-RC to a post-RC. The origin recognition complex (ORC), Cdc6p, and...

journal_title：Genes & development

authors： Liang C,Stillman B

更新日期：1997-12-15 00:00:00

abstract：:Adult mesenchymal stem cells (MSCs) derived from bone marrow contribute to the regeneration of multiple types of mesenchymal tissues. Here we describe the functional role of a novel form of cross-talk between the transforming growth factor beta1 (TGF-beta1) and Wnt signaling pathways in regulating the activities of hu...

journal_title：Genes & development

authors： Jian H,Shen X,Liu I,Semenov M,He X,Wang XF

更新日期：2006-03-15 00:00:00

abstract：:Lineage commitment studies in mammary glands have focused on identifying cell populations that display stem or progenitor properties. However, the mechanisms that control cell fate have been incompletely explored. Herein we show that zinc finger protein 157 (Zfp157) is required to establish the balance between luminal...

journal_title：Genes & development

authors： Oliver CH,Khaled WT,Frend H,Nichols J,Watson CJ

更新日期：2012-05-15 00:00:00

abstract：:MicF, one of the first chromosomally encoded regulatory small RNAs (sRNAs) to be discovered, was found to modulate the expression of OmpF, an abundant outer membrane protein. Several recent papers have now shown that this is not an isolated case. At least five other sRNAs also regulate the synthesis of outer membrane ...

journal_title：Genes & development

authors： Guillier M,Gottesman S,Storz G

更新日期：2006-09-01 00:00:00

abstract：:Genomic imprinting is an epigenetic mechanism by which genes are expressed in a parental origin-dependent manner. We recently discovered that, like DNA methylation, oocyte-inherited H3K27me3 can also serve as an imprinting mark in mouse preimplantation embryos. In this study, we found H3K27me3 is strongly biased towar...

journal_title：Genes & development

authors： Zhang W,Chen Z,Yin Q,Zhang D,Racowsky C,Zhang Y

更新日期：2019-04-01 00:00:00

abstract：:The evolutionarily conserved checkpoint protein kinase, TOR (target of rapamycin), has emerged as a major effector of cell growth and proliferation via the regulation of protein synthesis. Work in the last decade clearly demonstrates that TOR controls protein synthesis through a stunning number of downstream targets. ...

journal_title：Genes & development

authors： Hay N,Sonenberg N

更新日期：2004-08-15 00:00:00

abstract：:Eukaryotic chromosomes reach their stable rod-shaped appearance in mitosis in a reaction dependent on the evolutionarily conserved condensin complex. Little is known about how and where condensin associates with chromosomes. Here, we analyze condensin binding to budding yeast chromosomes using high-resolution oligonuc...

journal_title：Genes & development

authors： D'Ambrosio C,Schmidt CK,Katou Y,Kelly G,Itoh T,Shirahige K,Uhlmann F

更新日期：2008-08-15 00:00:00

abstract：:Chromatin structure is believed to be important for a number of cellular processes, including transcription. However, the role of nucleosomes in transcription is not well understood. We have identified the yeast histone locus HTB1-HTB1, encoding histones H2A and H2B, as a suppressor of solo delta insertion mutations t...

journal_title：Genes & development

authors： Clark-Adams CD,Norris D,Osley MA,Fassler JS,Winston F

更新日期：1988-02-01 00:00:00

abstract：:Pancreatic ductal adenocarcinoma (PDA) develops predominantly through pancreatic intraepithelial neoplasia (PanIN) and intraductal papillary mucinous neoplasm (IPMN) precursor lesions. Pancreatic acinar cells are reprogrammed to a "ductal-like" state during PanIN-PDA formation. Here, we demonstrate a parallel mechanis...

journal_title：Genes & development

authors： Roy N,Malik S,Villanueva KE,Urano A,Lu X,Von Figura G,Seeley ES,Dawson DW,Collisson EA,Hebrok M

更新日期：2015-03-15 00:00:00

abstract：:Pcf11 is one of numerous proteins involved in pre-mRNA 3'-end processing and transcription termination. Using elongation complexes (ECs) formed from purified yeast RNA polymerase II (Pol II), we show that a 140-amino acid polypeptide from yeast Pcf11 is capable of dismantling the EC in vitro. This action depends on th...

journal_title：Genes & development

authors： Zhang Z,Fu J,Gilmour DS

更新日期：2005-07-01 00:00:00

abstract：:DNA methylation is a key regulator of embryonic stem cell (ESC) biology, dynamically changing between naïve, primed, and differentiated states. The p53 tumor suppressor is a pivotal guardian of genomic stability, but its contributions to epigenetic regulation and stem cell biology are less explored. We report that, in...

journal_title：Genes & development

authors： Tovy A,Spiro A,McCarthy R,Shipony Z,Aylon Y,Allton K,Ainbinder E,Furth N,Tanay A,Barton M,Oren M

更新日期：2017-05-15 00:00:00

abstract：:Using an in vitro system in which a 5' splice site (5'SS) RNA oligo (AAG decreases GUAAGUAdT) is capable of inducing formation of U2/U4/U5/U6 snRNP complex we show that this oligo specifically binds to U4/U5/U6 snRNP and cross-links to U6 snRNA in the absence of U2 snRNP. Moreover, 5'SS RNA oligo bound to U4/U5/U6 snR...

journal_title：Genes & development

authors： Konforti BB,Konarska MM

更新日期：1994-08-15 00:00:00