Abstract：
:Our laboratory has been utilizing the Escherichia coli outer membrane protein LamB to study the mechanism of protein localization. Various lines of evidence suggest that, in addition to a signal sequence, regions within the mature protein are required for efficient localization. In particular, studies using LamB-LacZ hybrid proteins have identified regions between amino acids 27 and 49 of mature LamB, which may play an important role in localization. To elucidate further the function of these regions, a series of in-frame deletions that remove varying lengths of early lamB sequences was constructed. The effects of these deletions on export of a large LamB-LacZ hybrid protein, 42-1, and on export of an otherwise wild-type LamB protein were determined. We find a strong correlation between the sequences deleted and the export phenotypes these deletions impart to both LamB and the LamB-LacZ42-1 hybrid protein. On the basis of these findings, the deletions can be divided into several distinct classes that define a region within mature LamB that participates in localization. This region extends amino terminally from amino acid 28 of the mature protein and functions in the rapid and efficient localization of LamB from the cytoplasm.
journal_name
Genes Devjournal_title
Genes & developmentauthors
Rasmussen BA,Silhavy TJdoi
10.1101/gad.1.2.185subject
Has Abstractpub_date
1987-04-01 00:00:00pages
185-96issue
2eissn
0890-9369issn
1549-5477journal_volume
1pub_type
杂志文章abstract：:The mechanisms by which homeoproteins bind selectively to target genes in vivo have long remained unresolved. Here we report that PIAS1 confers DNA-binding specificity on the Msx1 homeoprotein by regulating its subnuclear localization and proximity to target genes. We demonstrate that the interaction of Msx1 with PIAS...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1392006
更新日期：2006-04-01 00:00:00
abstract：:The bHLH-PAS transcription factor SIM1 is expressed during the development of the hypothalamic-pituitary axis in three hypothalamic nuclei: the paraventricular nucleus (PVN), the anterior periventricular nucleus (aPV), and the supraoptic nucleus (SON). To investigate Sim1 function in the hypothalamus, we produced mice...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.12.20.3264
更新日期：1998-10-15 00:00:00
abstract：:Until recently it was believed that Xenopus oocytes respond to heat shock by synthesizing the 70-kD heat shock protein hsp70 and that, uniquely amongst animal cell types, this response is mediated entirely at the translational level. This view has now been challenged and we present data that reevaluate the involvement...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1.5.433
更新日期：1987-07-01 00:00:00
abstract：:AMP-activated protein kinase (AMPK) is a sensor of energy status that maintains cellular energy homeostasis. It arose very early during eukaryotic evolution, and its ancestral role may have been in the response to starvation. Recent work shows that the kinase is activated by increases not only in AMP, but also in ADP....
journal_title：Genes & development
pub_type： 杂志文章,评审
doi：10.1101/gad.17420111
更新日期：2011-09-15 00:00:00
abstract：:In many eukaryotes, siRNAs bound to Argonaute proteins guide chromatin-modifying enzymes to complementary loci, resulting in transcriptional gene silencing. Multiple lines of evidence indicate that siRNAs base-pair with longer RNAs produced at target loci, but the possibility that siRNAs base-pair directly with DNA re...
journal_title：Genes & development
pub_type： 评论,杂志文章
doi：10.1101/gad.294900.116
更新日期：2016-12-15 00:00:00
abstract：:The bcl-6 proto-oncogene encodes a POZ/zinc finger transcriptional repressor expressed in germinal center (GC) B and T cells and required for GC formation and antibody affinity maturation. Deregulation of bcl-6 expression by chromosomal rearrangements and point mutations of the bcl-6 promoter region are implicated in ...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.12.13.1953
更新日期：1998-07-01 00:00:00
abstract：:Chromosome segregation requires stable bipolar attachments of spindle microtubules to kinetochores. The dynein/dynactin motor complex localizes transiently to kinetochores and is implicated in chromosome segregation, but its role remains poorly understood. Here, we use the Caenorhabditis elegans embryo to investigate ...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1687508
更新日期：2008-09-01 00:00:00
abstract：:The gene for an essential protein subunit of nuclear RNase P from Saccharomyces cerevisiae has been cloned. The gene for this protein, RPP1, was identified by virtue of its homology with a human scleroderma autoimmune antigen, Rpp30, which copurifies with human RNase P. Epitope-tagged Rpp1 can be found in association ...
journal_title：Genes & development
pub_type： 更正并重新发布的文章,杂志文章
doi：10.1101/gad.11.21.2926
更新日期：1997-11-01 00:00:00
abstract：:Genomic imprinting is an epigenetic mechanism by which genes are expressed in a parental origin-dependent manner. We recently discovered that, like DNA methylation, oocyte-inherited H3K27me3 can also serve as an imprinting mark in mouse preimplantation embryos. In this study, we found H3K27me3 is strongly biased towar...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.323105.118
更新日期：2019-04-01 00:00:00
abstract：:Gene inactivation can result when a delta element of the Ty1 transposon inserted into the yeast HIS4 promoter (his4-912delta) alters the transcription initiation site. Previous work has identified mutations that suppress this transcriptional defect by restoring the transcription start site to the native position, and ...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.10.5.604
更新日期：1996-03-01 00:00:00
abstract：:How muscle diversity is generated in the vertebrate body is poorly understood. In the limb, dorsal and ventral muscle masses constitute the first myogenic diversification, as each gives rise to distinct muscles. Myogenesis initiates after muscle precursor cells (MPCs) have migrated from the somites to the limb bud and...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.187807.112
更新日期：2012-09-15 00:00:00
abstract：:In Saccharomyces cerevisiae, gene silencing at the HMR and HML loci is normally dependent on Sir2p, Sir3p, and Sir4p, which are structural components of silenced chromatin. Sir2p is a NAD+-dependent histone deacetylase required for silencing. Silencing can be restored in cells lacking Sir proteins by a dominant mutati...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.873601
更新日期：2001-04-15 00:00:00
abstract：:Extensive DNA elimination via a DNA breakage and rejoining process occurs during macronuclear development in the hypotrich ciliate Euplotes crassus. The excision process involves the removal of short, unique segments of DNA (internal eliminated sequences; IESs) and at least two highly repetitive families of transposon...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.7.1.84
更新日期：1993-01-01 00:00:00
abstract：:We demonstrate here that the previously described bacterial promoter upstream element (UP element) consists of two distinct subsites, each of which, by itself, can bind the RNA polymerase holoenzyme alpha subunit carboxy-terminal domain (RNAP alphaCTD) and stimulate transcription. Using binding-site-selection experime...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.13.16.2134
更新日期：1999-08-15 00:00:00
abstract：:Gastrulation in mice is associated with the start of extreme proliferation and differentiation. The potential cost to the embryo of a very rapid proliferation rate is a high production of damaged cells. We demonstrate a novel surveillance mechanism for the elimination of cells damaged by ionizing radiation during mous...
journal_title：Genes & development
pub_type： 杂志文章
doi：
更新日期：2000-08-15 00:00:00
abstract：:By studying neuroectoderm formation in the absence of mesoderm and mesectoderm in mutants of the zygotic genes snail and twist, we have found that the number of neuroblasts is not reduced in these mutants, suggesting that mesoderm and mesectoderm are not essential for the initiation of neural development. The position...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.5.9.1577
更新日期：1991-09-01 00:00:00
abstract：:Changes in the pattern of gene expression play an important role in allowing cancer cells to acquire their hallmark characteristics, while genomic instability enables cells to acquire genetic alterations that promote oncogenesis. Chromatin plays central roles in both transcriptional regulation and the maintenance of g...
journal_title：Genes & development
pub_type： 杂志文章,评审
doi：10.1101/gad.255182.114
更新日期：2015-02-01 00:00:00
abstract：:Mammalian development requires effective mechanisms to repress genes whose expression would generate inappropriately specified cells. The Polycomb-repressive complex 1 (PRC1) family complexes are central to maintaining this repression. These include a set of canonical PRC1 complexes, each of which contains four core p...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.326488.119
更新日期：2019-07-01 00:00:00
abstract：:Two independent two-dimensional (2D) gel methods were used to map an origin of replication that is developmentally regulated by the steroid hormone ecdysone, namely an origin for DNA puff amplification in the fungus fly Sciara coprophila. Initiation of replication was found to occur within a small region of no larger ...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.7.6.1072
更新日期：1993-06-01 00:00:00
abstract：:The neurofibromatosis type II (NF2) tumor suppressor encodes a putative cytoskeletal associated protein, the loss of which leads to the development of Schwann cell tumors associated with NF2 in humans. The NF2 protein merlin belongs to the band 4.1 family of proteins that link membrane proteins to the cytoskeleton and...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.11.10.1253
更新日期：1997-05-15 00:00:00
abstract：:In the yeast Saccharomyces cerevisiae, sequence-specific DNA binding by the origin recognition complex (ORC) is responsible for selecting origins of DNA replication. In metazoans, origin selection is poorly understood and it is unknown whether specific DNA binding by metazoan ORC controls replication. To address this ...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.13.20.2639
更新日期：1999-10-15 00:00:00
abstract：:Many bacterial small regulatory RNAs (sRNAs) pair with mRNA targets, stimulating or inhibiting mRNA stability and/or translation. Regulation of these sRNAs is usually due to tight transcriptional regulation of synthesis.In this issue of Genes & Development and a related paper in Molecular Microbiology, Figueroa-Bossi ...
journal_title：Genes & development
pub_type： 评论,杂志文章
doi：10.1101/gad.1846609
更新日期：2009-09-01 00:00:00
abstract：:RNA polymerase II nascent transcripts are capped during pausing before elongation. Here we report that hSPT5, the human homolog of yeast elongation factor SPT5, interacts directly with the capping enzyme. hSPT5 stimulated capping enzyme guanylylation and mRNA capping by severalfold. Although RNA 5'-triphosphatase acti...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.13.14.1774
更新日期：1999-07-15 00:00:00
abstract：:Nuclear pore complexes (NPCs) emerged as nuclear transport channels in eukaryotic cells ∼1.5 billion years ago. While the primary role of NPCs is to regulate nucleo-cytoplasmic transport, recent research suggests that certain NPC proteins have additionally acquired the role of affecting gene expression at the nuclear ...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.280941.116
更新日期：2016-05-15 00:00:00
abstract：:The mechanisms of RNA replication of plus-strand RNA viruses are still unclear. Here, we identified the first promoter element for RNA synthesis described in a flavivirus. Using dengue virus as a model, we found that the viral RdRp discriminates the viral RNA by specific recognition of a 5' element named SLA. We demon...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1444206
更新日期：2006-08-15 00:00:00
abstract：:The mammalian high mobility group proteins HMG1 and HMG2 are abundant, chromatin-associated proteins whose cellular function is not known. In this study we show that these proteins can substitute for the prokaryotic DNA-bending protein HU in promoting the assembly of the Hin invertasome, an intermediate structure in H...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.7.8.1521
更新日期：1993-08-01 00:00:00
abstract：:The crucifer Arabidopsis thaliana has been used widely as a model organism for the study of plant development. We describe here the development of an efficient insertional mutagenesis system in Arabidopsis that permits identification of genes by their patterns of expression during development. Transposable elements of...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.9.14.1797
更新日期：1995-07-15 00:00:00
abstract：:The transcription factor Ebf1 is an important determinant of early B lymphopoiesis. To gain insight into the functions of Ebf1 at distinct stages of differentiation, we conditionally inactivated Ebf1. We found that Ebf1 is required for the proliferation, survival, and signaling of pro-B cells and peripheral B-cell sub...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.187328.112
更新日期：2012-04-01 00:00:00
abstract：:Gene duplication and divergence is a major driver in the emergence of evolutionary novelties. How variations in amino acid sequences lead to loss of ancestral activity and functional diversification of proteins is poorly understood. We used cross-species functional analysis of Drosophila Labial and its mouse HOX1 orth...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.342329.120
更新日期：2020-12-01 00:00:00
abstract：:The PWI motif is a highly conserved domain of unknown function in the SRm160 splicing and 3'-end cleavage-stimulatory factor, as well as in several other known or putative pre-mRNA processing components. We show here that the PWI motif is a new type of RNA/DNA-binding domain that has an equal preference for single- an...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1060403
更新日期：2003-02-15 00:00:00
