Conversion of a gene-specific repressor to a regional silencer.

abstract：:AAUAAA is the most highly conserved motif in eukaryotic mRNA polyadenylation sites and, in mammals, is specifically recognized by the multisubunit CPSF (cleavage and polyadenylation specificity factor) complex. Despite its critical functions in mRNA 3' end formation, the molecular basis for CPSF-AAUAAA interaction rem...

journal_title：Genes & development

authors： Chan SL,Huppertz I,Yao C,Weng L,Moresco JJ,Yates JR 3rd,Ule J,Manley JL,Shi Y

更新日期：2014-11-01 00:00:00

abstract：:The ETS transcription factors perform distinct biological functions despite conserving a highly similar DNA-binding domain. One distinguishing property of a subset of ETS proteins is a conserved region of 80 amino acids termed the Pointed (PNT) domain. Using enzyme kinetics we determined that the Ets-1 PNT domain cont...

journal_title：Genes & development

authors： Seidel JJ,Graves BJ

更新日期：2002-01-01 00:00:00

abstract：:The SNF1 protein kinase is broadly conserved in eukaryotes and has been implicated in responses to environmental and nutritional stress. In yeast, the SNF1 kinase has a central role in the response to glucose starvation. SNF1 is associated with its activating subunit, SNF4, and other proteins in complexes. Using the t...

journal_title：Genes & development

authors： Jiang R,Carlson M

更新日期：1996-12-15 00:00:00

abstract：:The organizer region, or dorsal blastopore lip, plays a central role in the initiation of gastrulation and the formation of the body axis during Xenopus development. A similar process can also be induced in ectodermal explants by activin or by injection of activin mRNA into embryos. We have searched early embryo-speci...

journal_title：Genes & development

authors： Dirksen ML,Jamrich M

更新日期：1992-04-01 00:00:00

abstract：:Expression of the Arabidopsis CGS1 gene that codes for cystathionine gamma-synthase is feedback regulated at the step of mRNA stability in response to S-adenosyl-L-methionine (AdoMet). A short stretch of amino acid sequence, called the MTO1 region, encoded by the first exon of CGS1 itself is involved in this regulatio...

journal_title：Genes & development

authors： Onouchi H,Nagami Y,Haraguchi Y,Nakamoto M,Nishimura Y,Sakurai R,Nagao N,Kawasaki D,Kadokura Y,Naito S

更新日期：2005-08-01 00:00:00

abstract：:The bHLH-PAS transcription factor SIM1 is expressed during the development of the hypothalamic-pituitary axis in three hypothalamic nuclei: the paraventricular nucleus (PVN), the anterior periventricular nucleus (aPV), and the supraoptic nucleus (SON). To investigate Sim1 function in the hypothalamus, we produced mice...

journal_title：Genes & development

authors： Michaud JL,Rosenquist T,May NR,Fan CM

更新日期：1998-10-15 00:00:00

abstract：:Entry into sporulation by the Gram-positive bacterium Bacillus subtilis is governed by two transcription factors, Spo0A and sigma H, and involves a switch in the site of division from a medial to a polar location. We report that at the onset of sporulation, assembly of the cell division protein FtsZ shifts from midcel...

journal_title：Genes & development

authors： Levin PA,Losick R

更新日期：1996-02-15 00:00:00

abstract：:Cell cycle checkpoints are critical for genomic stability. Rad17, a component of the checkpoint clamp loader complex (Rad17/Rfc2-5), is required for the response to DNA damage and replication stress. To explore the role of Rad17 in the maintenance of genomic integrity, we established somatic conditional alleles of RAD...

journal_title：Genes & development

authors： Wang X,Zou L,Zheng H,Wei Q,Elledge SJ,Li L

更新日期：2003-04-15 00:00:00

abstract：:We have studied the sequence requirements for 3'-end formation of rDNA transcripts in a cell-free system and show that the generation of correct ends of mouse pre-rRNA is brought about by a two-step process that involves a bona fide termination reaction, followed by a specific trimming of the primary transcript by 10 ...

journal_title：Genes & development

authors： Kuhn A,Grummt I

更新日期：1989-02-01 00:00:00

abstract：:The removal of the 5' cap structure by the decapping enzyme DCP2 inhibits translation and generally commits the mRNA to irreversible 5'-to-3' exonucleolytic degradation by XRN1. DCP2 catalytic activity is stimulated by DCP1, and these proteins form the conserved core of the decapping complex. Additional decapping fact...

journal_title：Genes & development

authors： Jonas S,Izaurralde E

更新日期：2013-12-15 00:00:00

abstract：:In Drosophila germ cells, PIWI-interacting RNAs (piRNAs) are amplified through a PIWI slicer-dependent feed-forward loop termed the ping-pong cycle, yielding secondary piRNAs. However, the detailed mechanism remains poorly understood, largely because an ex vivo model system amenable to biochemical analyses has not bee...

journal_title：Genes & development

authors： Sumiyoshi T,Sato K,Yamamoto H,Iwasaki YW,Siomi H,Siomi MC

更新日期：2016-07-15 00:00:00

abstract：:Noncoding RNA regulators have been implicated in almost all imaginable cellular processes. Here we review how regulatory small RNAs such as Spot42, SgrS, GlmY, and GlmZ and a cis-encoded ribozyme in glmS mRNA control sugar metabolism. Besides discussing the physiological implications, we show how the study of these mo...

journal_title：Genes & development

authors： Görke B,Vogel J

更新日期：2008-11-01 00:00:00

abstract：:The labial (lab) gene of Drosophila melanogaster is necessary for the proper development of the embryonic (larval) and adult head. We have identified the lab transcription unit within the proximal portion of the Antennapedia Complex (ANT-C) by mapping the molecular lesions associated with chromosomally rearranged lab ...

journal_title：Genes & development

authors： Diederich RJ,Merrill VK,Pultz MA,Kaufman TC

更新日期：1989-03-01 00:00:00

abstract：:The senescence-associated secretory phenotype (SASP) is a major trait of senescent cells, but the molecular regulators of SASP factor secretion are poorly understood. Mass spectrometry analysis revealed that secretory carrier membrane protein 4 (SCAMP4) levels were strikingly elevated on the surface of senescent cells...

journal_title：Genes & development

authors： Kim KM,Noh JH,Bodogai M,Martindale JL,Pandey PR,Yang X,Biragyn A,Abdelmohsen K,Gorospe M

更新日期：2018-07-01 00:00:00

abstract：:The nuclear receptor Rev-erbα regulates circadian rhythm and metabolism, but its effects are modest and it has been considered to be a secondary regulator of the cell-autonomous clock. Here we report that depletion of Rev-erbα together with closely related Rev-erbβ has dramatic effects on the cell-autonomous clock as ...

journal_title：Genes & development

authors： Bugge A,Feng D,Everett LJ,Briggs ER,Mullican SE,Wang F,Jager J,Lazar MA

更新日期：2012-04-01 00:00:00

abstract：:In many eukaryotes, siRNAs bound to Argonaute proteins guide chromatin-modifying enzymes to complementary loci, resulting in transcriptional gene silencing. Multiple lines of evidence indicate that siRNAs base-pair with longer RNAs produced at target loci, but the possibility that siRNAs base-pair directly with DNA re...

journal_title：Genes & development

authors： Wendte JM,Pikaard CS

更新日期：2016-12-15 00:00:00

abstract：:The ATR (ATM and Rad3-related) kinase and its regulatory partner ATRIP (ATR-interacting protein) coordinate checkpoint responses to DNA damage and replication stress. TopBP1 functions as a general activator of ATR. However, the mechanism by which TopBP1 activates ATR is unknown. Here, we show that ATRIP contains a Top...

journal_title：Genes & development

authors： Mordes DA,Glick GG,Zhao R,Cortez D

更新日期：2008-06-01 00:00:00

abstract：:One of the central requirements for eukaryotic chromosome stability is the maintenance of the simple sequence tracts at telomeres. In this study, we use genetic and physical assays to reveal the nature of a novel mechanism by which telomere length is controlled. This mechanism, telomeric rapid deletion (TRD), is capab...

journal_title：Genes & development

authors： Li B,Lustig AJ

更新日期：1996-06-01 00:00:00

abstract：:The superoxide radical (O(2)(-)) has long been considered a major cause of aging. O(2)(-) in cytosolic, extracellular, and mitochondrial pools is detoxified by dedicated superoxide dismutase (SOD) isoforms. We tested the impact of each SOD isoform in Caenorhabditis elegans by manipulating its five sod genes and saw no...

journal_title：Genes & development

authors： Doonan R,McElwee JJ,Matthijssens F,Walker GA,Houthoofd K,Back P,Matscheski A,Vanfleteren JR,Gems D

更新日期：2008-12-01 00:00:00

abstract：:Caenorhabditis elegans GLD-1, a KH motif containing RNA-binding protein of the GSG/STAR subfamily, controls diverse aspects of germ line development, suggesting that it may have multiple mRNA targets. We used an immunoprecipitation/subtractive hybridization/cloning strategy to identify 15 mRNAs that are putative targe...

journal_title：Genes & development

authors： Lee MH,Schedl T

更新日期：2001-09-15 00:00:00

abstract：:Activation of the CLB gene cluster through the assembly of Mcm1p-Fkh2p complexes at target promoters is essential for mitotic entry and transition through M phase. We show that the activation of this mitotic transcriptional program is dependent on the recruitment of Ndd1p, a coactivator that performs its essential fun...

journal_title：Genes & development

authors： Reynolds D,Shi BJ,McLean C,Katsis F,Kemp B,Dalton S

更新日期：2003-07-15 00:00:00

abstract：:Specific recognition of splice sites within metazoan mRNA precursors (pre-mRNAs) is a potential stage for gene regulation by alternative splicing. Splicing factors of the SR protein family play a major role in this regulation, as they are required for early recognition of splice sites during spliceosome assembly. Here...

journal_title：Genes & development

authors： Labourier E,Bourbon HM,Gallouzi IE,Fostier M,Allemand E,Tazi J

更新日期：1999-03-15 00:00:00

abstract：:The mechanisms of RNA replication of plus-strand RNA viruses are still unclear. Here, we identified the first promoter element for RNA synthesis described in a flavivirus. Using dengue virus as a model, we found that the viral RdRp discriminates the viral RNA by specific recognition of a 5' element named SLA. We demon...

journal_title：Genes & development

authors： Filomatori CV,Lodeiro MF,Alvarez DE,Samsa MM,Pietrasanta L,Gamarnik AV

更新日期：2006-08-15 00:00:00

abstract：:In petunia flowers, the loci an1, an2, and an11 control the pigmentation of the flower by stimulating the transcription of anthocyanin biosynthetic genes. The an1 and an2 locus were recently cloned and encode a basic helix-loop-helix (bHLH) and MYB-domain transcriptional activator, respectively. Here, we report the is...

journal_title：Genes & development

authors： de Vetten N,Quattrocchio F,Mol J,Koes R

更新日期：1997-06-01 00:00:00

abstract：:The Bloom's syndrome gene (BLM) plays a pivotal role in the maintenance of genomic stability in somatic cells. It encodes a DNA helicase (BLM) of the RecQ family, but the exact function of BLM remains elusive. To study this question, we have cloned the BLM homolog of the frog Xenopus laevis (xBLM) and have raised anti...

journal_title：Genes & development

authors： Liao S,Graham J,Yan H

更新日期：2000-10-15 00:00:00

abstract：:Neuronal depolarization and CaM kinase IV signaling alter the splicing of multiple exons in transcripts for ion channels, neurotransmitter receptors, and other synaptic proteins. These splicing changes are mediated in part by special CaM kinase-responsive RNA elements, within or adjacent to exons that are repressed in...

journal_title：Genes & development

authors： Lee JA,Tang ZZ,Black DL

更新日期：2009-10-01 00:00:00

abstract：:Class switch recombination (CSR) diversifies antibodies for productive immune responses while maintaining stability of the B-cell genome. Transcription at the immunoglobulin heavy chain (Igh) locus targets CSR-associated DNA damage and is promoted by the BRCT domain-containing PTIP (Pax transactivation domain-interact...

journal_title：Genes & development

authors： Starnes LM,Su D,Pikkupeura LM,Weinert BT,Santos MA,Mund A,Soria R,Cho YW,Pozdnyakova I,Kubec Højfeldt M,Vala A,Yang W,López-Méndez B,Lee JE,Peng W,Yuan J,Ge K,Montoya G,Nussenzweig A,Choudhary C,Daniel JA

更新日期：2016-01-15 00:00:00

abstract：:The human T cell-specific transcription factor TCF-1 alpha plays a key role in the tissue-specific activation of the T cell receptor (TCR) C alpha enhancer and binds to pyrimidine-rich elements (5'-PyCTTTG-3') present in a variety of other T cell-specific control regions. Using amino acid sequence information derived ...

journal_title：Genes & development

authors： Waterman ML,Fischer WH,Jones KA

更新日期：1991-04-01 00:00:00

abstract：:We hypothesized that basic helix-loop-helix (bHLH) MIST1 (BHLHA15) is a "scaling factor" that universally establishes secretory morphology in cells that perform regulated secretion. Here, we show that targeted deletion of MIST1 caused dismantling of the secretory apparatus of diverse exocrine cells. Parietal cells (PC...

journal_title：Genes & development

authors： Lo HG,Jin RU,Sibbel G,Liu D,Karki A,Joens MS,Madison BB,Zhang B,Blanc V,Fitzpatrick JA,Davidson NO,Konieczny SF,Mills JC

更新日期：2017-01-15 00:00:00

abstract：:Pre-mRNA splicing is essential for generating mature mRNA and is also important for subsequent mRNA export and quality control. The splicing history is imprinted on spliced mRNA through the deposition of a splicing-dependent multiprotein complex, the exon junction complex (EJC), at approximately 20 nucleotides upstrea...

journal_title：Genes & development

authors： Shi H,Xu RM

更新日期：2003-04-15 00:00:00