Abstract:
:In Drosophila germ cells, PIWI-interacting RNAs (piRNAs) are amplified through a PIWI slicer-dependent feed-forward loop termed the ping-pong cycle, yielding secondary piRNAs. However, the detailed mechanism remains poorly understood, largely because an ex vivo model system amenable to biochemical analyses has not been available. Here, we show that CRISPR-mediated loss of function of lethal (3) malignant brain tumor [l(3)mbt] leads to ectopic activation of the germ-specific ping-pong cycle in ovarian somatic cells. Perinuclear foci resembling nuage, the ping-pong center, appeared following l(3)mbt mutation. This activation of the ping-pong machinery in cultured cells will greatly facilitate elucidation of the mechanism underlying secondary piRNA biogenesis in Drosophila.
journal_name
Genes Devjournal_title
Genes & developmentauthors
Sumiyoshi T,Sato K,Yamamoto H,Iwasaki YW,Siomi H,Siomi MCdoi
10.1101/gad.283929.116subject
Has Abstractpub_date
2016-07-15 00:00:00pages
1617-22issue
14eissn
0890-9369issn
1549-5477pii
30/14/1617journal_volume
30pub_type
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