Abstract:
:Somitogenesis involves the segmentation of the paraxial mesoderm into units along the anteroposterior axis. Here we show a role for Eph and ephrin signaling in the patterning of presomitic mesoderm and formation of the somites. Ephrin-A-L1 and ephrin-B2 are expressed in an iterative manner in the developing somites and presomitic mesoderm, as is the Eph receptor EphA4. We have examined the role of these proteins by injection of RNA, encoding dominant negative forms of Eph receptors and ephrins. Interruption of Eph signaling leads to abnormal somite boundary formation and reduced or disturbed myoD expression in the myotome. Disruption of Eph family signaling delays the normal down-regulation of her1 and Delta D expression in the anterior presomitic mesoderm and disrupts myogenic differentiation. We suggest that Eph signaling has a key role in the translation of the patterning of presomitic mesoderm into somites.
journal_name
Genes Devjournal_title
Genes & developmentauthors
Durbin L,Brennan C,Shiomi K,Cooke J,Barrios A,Shanmugalingam S,Guthrie B,Lindberg R,Holder Ndoi
10.1101/gad.12.19.3096subject
Has Abstractpub_date
1998-10-01 00:00:00pages
3096-109issue
19eissn
0890-9369issn
1549-5477journal_volume
12pub_type
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