I-Rel: a novel rel-related protein that inhibits NF-kappa B transcriptional activity.

Abstract:

:The NF-kappa B transcription factor complex is comprised of two subunits, p50 and p65, that share significant homology to the rel oncogene. We have isolated a cDNA encoding a novel 66-kD rel-related protein, designated I-Rel. Unlike other rel-related proteins, I-Rel does not interact with DNA. I-Rel forms heterodimers with p50, however, and greatly attenuates its DNA-binding activity--an effect probably resulting from the presence of a domain inhibitory to DNA binding present within the 121 amino-terminal residues of I-Rel. In contrast, I-Rel does not associate with p65. Transfection experiments demonstrate that I-Rel suppresses NF-kappa B-induced transcription, probably through its association with p50. Expression of I-Rel mRNA is induced by mitogenic stimulation and accumulates after the appearance of p50 transcripts. Our findings suggest that p50 and I-Rel are components of a feedback pathway where expression of I-Rel may modulate indirectly the expression of genes responsive to the NF-kappa B transcription factor complex.

journal_name

Genes Dev

journal_title

Genes & development

authors

Ruben SM,Klement JF,Coleman TA,Maher M,Chen CH,Rosen CA

doi

10.1101/gad.6.5.745

subject

Has Abstract

pub_date

1992-05-01 00:00:00

pages

745-60

issue

5

eissn

0890-9369

issn

1549-5477

journal_volume

6

pub_type

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