当前位置： SCI文献检索 > GENES & DEVELOPMENT期刊下所有文献 > UPF1 helicase promotes TSN-mediated miRNA decay.

UPF1 helicase promotes TSN-mediated miRNA decay.

Abstract：

:While microRNAs (miRNAs) regulate the vast majority of protein-encoding transcripts, little is known about how miRNAs themselves are degraded. We recently described Tudor-staphylococcal/micrococcal-like nuclease (TSN)-mediated miRNA decay (TumiD) as a cellular pathway in which the nuclease TSN promotes the decay of miRNAs that contain CA and/or UA dinucleotides. While TSN-mediated degradation of either protein-free or AGO2-loaded miRNAs does not require the ATP-dependent RNA helicase UPF1 in vitro, we report here that cellular TumiD requires UPF1. Results from experiments using AGO2-loaded miRNAs in duplex with target mRNAs indicate that UPF1 can dissociate miRNAs from their mRNA targets, making the miRNAs susceptible to TumiD. miR-seq (deep sequencing of miRNAs) data reveal that the degradation of ∼50% of candidate TumiD targets in T24 human urinary bladder cancer cells is augmented by UPF1. We illustrate the physiological relevance by demonstrating that UPF1-augmented TumiD promotes the invasion of T24 cells in part by degrading anti-invasive miRNAs so as to up-regulate the expression of proinvasive proteins.

journal_name

Genes Dev

journal_title

Genes & development

authors

Elbarbary RA,Miyoshi K,Hedaya O,Myers JR,Maquat LE

doi

10.1101/gad.303537.117

subject

Has Abstract

pub_date

2017-07-15 00:00:00

pages

1483-1493

issue

14

eissn

0890-9369

issn

1549-5477

pii

gad.303537.117

journal_volume

31

pub_type

杂志文章

相关文献

GENES & DEVELOPMENT文献大全
  • Direct induction of G1-specific transcripts following reactivation of the Cdc28 kinase in the absence of de novo protein synthesis.

    abstract：:In Saccharomyces cerevisiae, the genes encoding the HO endonuclease, G1-specific cyclins CLN1 and CLN2, as well as most proteins involved in DNA synthesis, are periodically transcribed with maximal levels reached in late G1. For HO and the DNA replication genes, cell cycle stage-specific expression has been shown to b...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.6.4.557

    authors： Marini NJ,Reed SI

    更新日期：1992-04-01 00:00:00

  • Control of C. elegans hermaphrodite gonad size and shape by vab-3/Pax6-mediated regulation of integrin receptors.

    abstract：:Integrin receptors for extracellular matrix are critical for cell motility, but the signals that determine when to stop are not known. Analysis of distal tip cell (DTC) migration during gonadogenesis in Caenorhabditis elegans has revealed the importance of transcription factor vab-3/Pax6 in regulating the alpha integr...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.1534807

    authors： Meighan CM,Schwarzbauer JE

    更新日期：2007-07-01 00:00:00

  • Replication fork stalling and cell cycle arrest in UV-irradiated Escherichia coli.

    abstract：:Faithful duplication of the genome relies on the ability to cope with an imperfect template. We investigated replication of UV-damaged DNA in Escherichia coli and found that ongoing replication stops for at least 15-20 min before resuming. Undamaged origins of replication (oriC) continue to fire at the normal rate and...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.417607

    authors： Rudolph CJ,Upton AL,Lloyd RG

    更新日期：2007-03-15 00:00:00

  • Regulated assembly and disassembly of the yeast telomerase quaternary complex.

    abstract：:The enzyme telomerase, which elongates chromosome termini, is a critical factor in determining long-term cellular proliferation and tissue renewal. Hence, even small differences in telomerase levels can have substantial consequences for human health. In budding yeast, telomerase consists of the catalytic Est2 protein ...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.246256.114

    authors： Tucey TM,Lundblad V

    更新日期：2014-10-01 00:00:00

  • Chromatin remodeler CHD7 regulates the stem cell identity of human neural progenitors.

    abstract：:Multiple congenital disorders often present complex phenotypes, but how the mutation of individual genetic factors can lead to multiple defects remains poorly understood. In the present study, we used human neuroepithelial (NE) cells and CHARGE patient-derived cells as an in vitro model system to identify the function...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.301887.117

    authors： Chai M,Sanosaka T,Okuno H,Zhou Z,Koya I,Banno S,Andoh-Noda T,Tabata Y,Shimamura R,Hayashi T,Ebisawa M,Sasagawa Y,Nikaido I,Okano H,Kohyama J

    更新日期：2018-01-15 00:00:00

  • Developmental control of the G1 to S transition in Drosophila: cyclin Eis a limiting downstream target of E2F.

    abstract：:The E2F transcription factor is required for S phase in Drosophila. While it also triggers expression of replication genes at the G1-S transition, the relevance of this transcription is not clear because many of the induced gene products are sufficiently stable that new expression is not required for S phase. However,...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.9.12.1456

    authors： Duronio RJ,O'Farrell PH

    更新日期：1995-06-15 00:00:00

  • A condensin-like dosage compensation complex acts at a distance to control expression throughout the genome.

    abstract：:In many species, a dosage compensation complex (DCC) is targeted to X chromosomes of one sex to equalize levels of X-gene products between males (1X) and females (2X). Here we identify cis-acting regulatory elements that target the Caenorhabditis elegans X chromosome for repression by the DCC. The DCC binds to discret...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.1751109

    authors： Jans J,Gladden JM,Ralston EJ,Pickle CS,Michel AH,Pferdehirt RR,Eisen MB,Meyer BJ

    更新日期：2009-03-01 00:00:00

  • Expression of a retinoic acid response element-hsplacZ transgene defines specific domains of transcriptional activity during mouse embryogenesis.

    abstract：:Treatment with retinoic acid (RA) is known to produce complex teratogenic effects in vertebrates, and its presence in the developing embryo as an endogenous substance has led to the suggestion that RA might be a natural morphogenetic agent. Although our understanding of the molecular mechanism of RA action has improve...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.5.8.1333

    authors： Rossant J,Zirngibl R,Cado D,Shago M,Giguère V

    更新日期：1991-08-01 00:00:00

  • Kinetochores accelerate or delay APC/C activation by directing Cdc20 to opposing fates.

    abstract：:Mitotic duration is determined by activation of the anaphase-promoting complex/cyclosome (APC/C) bound to its coactivator, Cdc20. Kinetochores, the microtubule-interacting machines on chromosomes, restrain mitotic exit when not attached to spindle microtubules by generating a Cdc20-containing complex that inhibits the...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.302067.117

    authors： Kim T,Lara-Gonzalez P,Prevo B,Meitinger F,Cheerambathur DK,Oegema K,Desai A

    更新日期：2017-06-01 00:00:00

  • SMG6 interacts with the exon junction complex via two conserved EJC-binding motifs (EBMs) required for nonsense-mediated mRNA decay.

    abstract：:Nonsense-mediated mRNA decay (NMD) is a quality control mechanism that detects and degrades mRNAs containing premature stop codons (PTCs). In vertebrates, PTCs trigger efficient NMD when located upstream of an exon junction complex (EJC). Degradation of PTC-containing mRNAs requires the endonucleolytic activity of SMG...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.604610

    authors： Kashima I,Jonas S,Jayachandran U,Buchwald G,Conti E,Lupas AN,Izaurralde E

    更新日期：2010-11-01 00:00:00

  • Fission yeast genes that confer resistance to staurosporine encode an AP-1-like transcription factor and a protein kinase related to the mammalian ERK1/MAP2 and budding yeast FUS3 and KSS1 kinases.

    abstract：:Staurosporine, a potent inhibitor of protein kinase C, arrests fission yeast cell elongation specifically at a stage immediately after cell division. We isolated two genes, which, when carried on multicopy plasmids, confer drug resistance in fission yeast. One, spk1+, encodes a protein kinase highly similar (54% ident...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.5.1.60

    authors： Toda T,Shimanuki M,Yanagida M

    更新日期：1991-01-01 00:00:00

  • TBP, Mot1, and NC2 establish a regulatory circuit that controls DPE-dependent versus TATA-dependent transcription.

    abstract：:The RNA polymerase II core promoter is a structurally and functionally diverse transcriptional module. RNAi depletion and overexpression experiments revealed a genetic circuit that controls the balance of transcription from two core promoter motifs, the TATA box and the downstream core promoter element (DPE). In this ...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.1681808

    authors： Hsu JY,Juven-Gershon T,Marr MT 2nd,Wright KJ,Tjian R,Kadonaga JT

    更新日期：2008-09-01 00:00:00

  • Fis binding to the recombinational enhancer of the Hin DNA inversion system.

    abstract：:The recombinational enhancer of the Hin inversion system in Salmonella stimulates recombination in vitro 150-fold in the presence of the Escherichia coli host factor Fis. To gain an understanding of the roles of the enhancer and Fis in stimulating the Hin-mediated inversion reaction, we have used nuclease and chemical...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.1.8.762

    authors： Bruist MF,Glasgow AC,Johnson RC,Simon MI

    更新日期：1987-10-01 00:00:00

  • VHL-box and SOCS-box domains determine binding specificity for Cul2-Rbx1 and Cul5-Rbx2 modules of ubiquitin ligases.

    abstract：:The ECS (Elongin B/C-Cul2/Cul5-SOCS-box protein) complex is a member of a family of ubiquitin ligases that share a Cullin-Rbx module. SOCS-box proteins recruit substrates to the ECS complex and are linked to Cullin-Rbx via Elongin B/C. VHL has been implicated as a SOCS-box protein, but lacks a C-terminal sequence (dow...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.1252404

    authors： Kamura T,Maenaka K,Kotoshiba S,Matsumoto M,Kohda D,Conaway RC,Conaway JW,Nakayama KI

    更新日期：2004-12-15 00:00:00

  • The hexosamine biosynthetic pathway couples growth factor-induced glutamine uptake to glucose metabolism.

    abstract：:Glucose and glutamine serve as the two primary carbon sources in proliferating cells, and uptake of both nutrients is directed by growth factor signaling. Although either glucose or glutamine can potentially support mitochondrial tricarboxylic acid (TCA) cycle integrity and ATP production, we found that glucose depriv...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.1985910

    authors： Wellen KE,Lu C,Mancuso A,Lemons JM,Ryczko M,Dennis JW,Rabinowitz JD,Coller HA,Thompson CB

    更新日期：2010-12-15 00:00:00

  • Distinct activities of the DExD/H-box splicing factor hUAP56 facilitate stepwise assembly of the spliceosome.

    abstract：:The essential splicing factor human UAP56 (hUAP56) is a DExD/H-box protein known to promote prespliceosome assembly. Here, using a series of hUAP56 mutants that are defective for ATP-binding, ATP hydrolysis, or dsRNA unwindase/helicase activity, we assess the relative contributions of these biochemical functions to pr...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.1657308

    authors： Shen H,Zheng X,Shen J,Zhang L,Zhao R,Green MR

    更新日期：2008-07-01 00:00:00

  • A novel homeobox gene mediates the Dpp signal to establish functional specificity within target cells.

    abstract：:Morphogen gradients of secreted molecules play critical roles in the establishment of the spatial pattern of gene expression. During midgut development in Drosophila, secreted molecules of Decapentaplegic (Dpp) and Wingless (Wg) establish unique transcriptional regulation within target cells to specify the resultant c...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.12.17.2724

    authors： Nakagoshi H,Hoshi M,Nabeshima Y,Matsuzaki F

    更新日期：1998-09-01 00:00:00

  • Modulating the outer membrane with small RNAs.

    abstract：:MicF, one of the first chromosomally encoded regulatory small RNAs (sRNAs) to be discovered, was found to modulate the expression of OmpF, an abundant outer membrane protein. Several recent papers have now shown that this is not an isolated case. At least five other sRNAs also regulate the synthesis of outer membrane ...

    journal_title：Genes & development

    pub_type： 杂志文章,评审

    doi：10.1101/gad.1457506

    authors： Guillier M,Gottesman S,Storz G

    更新日期：2006-09-01 00:00:00

  • Autoregulation of TDP-43 mRNA levels involves interplay between transcription, splicing, and alternative polyA site selection.

    abstract：:TDP-43 is a critical RNA-binding factor associated with pre-mRNA splicing in mammals. Its expression is tightly autoregulated, with loss of this regulation implicated in human neuropathology. We demonstrate that TDP-43 overexpression in humans and mice activates a 3' untranslated region (UTR) intron, resulting in exci...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.194829.112

    authors： Avendaño-Vázquez SE,Dhir A,Bembich S,Buratti E,Proudfoot N,Baralle FE

    更新日期：2012-08-01 00:00:00

  • A Polycomb group protein complex with sequence-specific DNA-binding and selective methyl-lysine-binding activities.

    abstract：:Polycomb response elements (PREs) are specific cis-regulatory sequences needed for transcriptional repression of HOX and other target genes by Polycomb group (PcG) proteins. Among the many PcG proteins known in Drosophila, Pho is the only sequence-specific DNA-binding protein. To gain insight into the function of Pho,...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.377406

    authors： Klymenko T,Papp B,Fischle W,Köcher T,Schelder M,Fritsch C,Wild B,Wilm M,Müller J

    更新日期：2006-05-01 00:00:00

  • Cdc28-Clb5 (CDK-S) and Cdc7-Dbf4 (DDK) collaborate to initiate meiotic recombination in yeast.

    abstract：:S-phase cyclin-dependent kinase Cdc28-Clb5 (CDK-S) and Dbf4-dependent kinase Cdc7-Dbf4 (DDK) are highly conserved kinases well known for their roles in the initiation of DNA replication. CDK-S is also essential for initiation of meiotic recombination because it phosphorylates Ser30 of Mer2, a meiosis-specific double-s...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.1626408

    authors： Wan L,Niu H,Futcher B,Zhang C,Shokat KM,Boulton SJ,Hollingsworth NM

    更新日期：2008-02-01 00:00:00

  • Transcription factor CREM coordinates the timing of hepatocyte proliferation in the regenerating liver.

    abstract：:The liver regenerates upon partial hepatectomy (PH) as terminally differentiated hepatocytes undergo a tremendous proliferative process. CREM gene expression is powerfully induced during liver regeneration. We show that cell proliferation is significantly reduced upon PH in CREM-/- mice. There is a reduction in DNA sy...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.12.23.3639

    authors： Servillo G,Della Fazia MA,Sassone-Corsi P

    更新日期：1998-12-01 00:00:00

  • Fos-Jun interaction: mutational analysis of the leucine zipper domain of both proteins.

    abstract：:Jun and Fos oncoproteins form a complex that regulates transcription from promoters containing AP-1 binding sites. The 'leucine zipper' domain of both Fos and Jun is necessary for the formation of the heterodimer, but the role of specific leucine residues is unclear. We have used site-specific mutagenesis to examine t...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.3.6.770

    authors： Ransone LJ,Visvader J,Sassone-Corsi P,Verma IM

    更新日期：1989-06-01 00:00:00

  • Intercellular C-signaling in Myxococcus xanthus involves a branched signal transduction pathway.

    abstract：:C-factor, the product of the csgA gene, is a cell-surface associated short-range intercellular signaling protein in Myxococcus xanthus. C-factor is required for at least four responses during starvation-induced fruiting body morphogenesis: rippling, aggregation, sporulation, and full expression of the csgA gene, all o...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.10.6.740

    authors： Søgaard-Andersen L,Slack FJ,Kimsey H,Kaiser D

    更新日期：1996-03-15 00:00:00

  • A single transcription factor is sufficient to induce and maintain secretory cell architecture.

    abstract：:We hypothesized that basic helix-loop-helix (bHLH) MIST1 (BHLHA15) is a "scaling factor" that universally establishes secretory morphology in cells that perform regulated secretion. Here, we show that targeted deletion of MIST1 caused dismantling of the secretory apparatus of diverse exocrine cells. Parietal cells (PC...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.285684.116

    authors： Lo HG,Jin RU,Sibbel G,Liu D,Karki A,Joens MS,Madison BB,Zhang B,Blanc V,Fitzpatrick JA,Davidson NO,Konieczny SF,Mills JC

    更新日期：2017-01-15 00:00:00

  • WUSCHEL is a primary target for transcriptional regulation by SPLAYED in dynamic control of stem cell fate in Arabidopsis.

    abstract：:SNF2 chromatin-remodeling ATPases play an important role in ensuring proper development in higher eukaryotes by controlling accessibility of cis-regulatory DNA regions to transcription factors and to the transcriptional machinery. However, the biological targets controlled by these ATPases are largely unknown. Using g...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.1276305

    authors： Kwon CS,Chen C,Wagner D

    更新日期：2005-04-15 00:00:00

  • De- and repolarization mechanism of flagellar morphogenesis during a bacterial cell cycle.

    abstract：:Eukaryotic morphogenesis is seeded with the establishment and subsequent amplification of polarity cues at key times during the cell cycle, often using (cyclic) nucleotide signals. We discovered that flagellum de- and repolarization in the model prokaryote Caulobacter crescentus is precisely orchestrated through at le...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.222679.113

    authors： Davis NJ,Cohen Y,Sanselicio S,Fumeaux C,Ozaki S,Luciano J,Guerrero-Ferreira RC,Wright ER,Jenal U,Viollier PH

    更新日期：2013-09-15 00:00:00

  • Repression of p53-mediated transcription by MDM2: a dual mechanism.

    abstract：:The oncoprotein MDM2 binds to the activation domain of the tumor suppressor p53 and inhibits its ability to stimulate transcription. This same region of p53 is able to bind several basal transcription factors that appear to be important for the transactivation function of p53. It has therefore been suggested that MDM2...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.11.15.1974

    authors： Thut CJ,Goodrich JA,Tjian R

    更新日期：1997-08-01 00:00:00

  • Defects in Saccharomyces cerevisiae protein phosphatase type I activate the spindle/kinetochore checkpoint.

    abstract：:A conditional allele of type 1 protein phosphatase (glc7-129) in Saccharomyces cerevisiae causes first cycle arrest in G2/M, characterized by cells with a short spindle and high H1 kinase activity. Point-of-execution experiments indicate Glc7p function is required in G2/M just before anaphase for the completion of mit...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.13.5.517

    authors： Bloecher A,Tatchell K

    更新日期：1999-03-01 00:00:00

  • A variant limb deformity transcript expressed in the embryonic mouse limb defines a novel formin.

    abstract：:The formins constitute a set of protein isoforms encoded by the alternatively spliced transcripts arising from the limb deformity (ld) locus of the mouse. Mutations in this locus disrupt formation of the anteroposterior axis of the embryonic limb. Although ld transcripts are widely expressed during embryogenesis, we h...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.6.1.29

    authors： Jackson-Grusby L,Kuo A,Leder P

    更新日期：1992-01-01 00:00:00