当前位置： SCI文献检索 > GENES & DEVELOPMENT期刊下所有文献 > Half helical turn spacing changes convert a frog into a mouse rDNA promoter: a distant upstream domain determines the helix face of the initiation site.

Half helical turn spacing changes convert a frog into a mouse rDNA promoter: a distant upstream domain determines the helix face of the initiation site.

Abstract：

:Transcription of frog rDNA by mouse cell factors is the only documented exception to the observed species selectivity of rRNA gene expression. This heterologous transcription is authentic in that it uses the normal frog upstream and core promoter domains, as well as the normal mouse polymerase I transcription factors, but it initiates at residue -4. We now show that by introducing an insertion or deletion of approximately one-half helical turn anywhere within the 90-bp region between the upstream and core promoter domains, the initiation site moves to residue +1. Promoters bearing spacing changes of approximately one or two full helix turns do not initiate at residue +1, whereas a promoter with a one and one-half-turn helical turn spacing change again supports initiation at residue +1. Thus, the position of the upstream domain of the frog promoter shows a stereo-specific requirement relative to the core promoter domain and dictates the face of the DNA helix on which transcription initiates, 140 bp away. In contrast, relative to the core promoter domain, initiation can occur on either side of the DNA helix. Furthermore, the striking observation that several frog half helical turn spacing change mutants are stronger templates with the mouse factors than the homologous mouse rDNA suggests that the polymerase I transcriptional machinery of even distantly related species is far more similar than generally envisioned.

journal_name

Genes Dev

journal_title

Genes & development

authors

Pape LK,Windle JJ,Sollner-Webb B

doi

10.1101/gad.4.1.52

subject

Has Abstract

pub_date

1990-01-01 00:00:00

pages

52-62

issue

1

eissn

0890-9369

issn

1549-5477

journal_volume

4

pub_type

杂志文章

相关文献

GENES & DEVELOPMENT文献大全
  • A novel class of small RNAs in mouse spermatogenic cells.

    abstract：:Small noncoding RNAs, including small interfering RNAs (siRNAs) and micro RNAs (miRNAs) of approximately 21 nucleotides (nt) in length, have emerged as potent regulators of gene expression at both transcriptional and post-transcriptional levels in diverse organisms. Here we report the identification of a novel class o...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.1434406

    authors： Grivna ST,Beyret E,Wang Z,Lin H

    更新日期：2006-07-01 00:00:00

  • Hap2-Ino80-facilitated transcription promotes de novo establishment of CENP-A chromatin.

    abstract：:Centromeres are maintained epigenetically by the presence of CENP-A, an evolutionarily conserved histone H3 variant, which directs kinetochore assembly and hence centromere function. To identify factors that promote assembly of CENP-A chromatin, we affinity-selected solubilized fission yeast CENP-ACnp1 chromatin. All ...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.332536.119

    authors： Singh PP,Shukla M,White SA,Lafos M,Tong P,Auchynnikava T,Spanos C,Rappsilber J,Pidoux AL,Allshire RC

    更新日期：2020-02-01 00:00:00

  • The neurohormone orexin stimulates hypoxia-inducible factor-1 activity.

    abstract：:Orexin A and Orexin B (also known as hypocretins) are neuropeptides that bind two related G-coupled protein receptors (OXR1 and OXR2) and thus induce wakefulness, food consumption, and locomotion. Conversely, deletion of the orexin gene in mice produces a condition similar to canine and human narcolepsy. Despite the c...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.1584307

    authors： Sikder D,Kodadek T

    更新日期：2007-11-15 00:00:00

  • Autoregulation of TDP-43 mRNA levels involves interplay between transcription, splicing, and alternative polyA site selection.

    abstract：:TDP-43 is a critical RNA-binding factor associated with pre-mRNA splicing in mammals. Its expression is tightly autoregulated, with loss of this regulation implicated in human neuropathology. We demonstrate that TDP-43 overexpression in humans and mice activates a 3' untranslated region (UTR) intron, resulting in exci...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.194829.112

    authors： Avendaño-Vázquez SE,Dhir A,Bembich S,Buratti E,Proudfoot N,Baralle FE

    更新日期：2012-08-01 00:00:00

  • A thymus-specific member of the HMG protein family regulates the human T cell receptor C alpha enhancer.

    abstract：:The human T cell-specific transcription factor TCF-1 alpha plays a key role in the tissue-specific activation of the T cell receptor (TCR) C alpha enhancer and binds to pyrimidine-rich elements (5'-PyCTTTG-3') present in a variety of other T cell-specific control regions. Using amino acid sequence information derived ...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.5.4.656

    authors： Waterman ML,Fischer WH,Jones KA

    更新日期：1991-04-01 00:00:00

  • A zinc-binding site in the largest subunit of DNA-dependent RNA polymerase is involved in enzyme assembly.

    abstract：:All multisubunit DNA-dependent RNA polymerases (RNAP) are zinc metalloenzymes, and at least two zinc atoms are present per enzyme molecule. RNAP residues involved in zinc binding and the functional role of zinc ions in the transcription mechanism or RNAP structure are unknown. Here, we locate four cysteine residues in...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.13.18.2439

    authors： Markov D,Naryshkina T,Mustaev A,Severinov K

    更新日期：1999-09-15 00:00:00

  • Rad54 and DNA Ligase IV cooperate to maintain mammalian chromatid stability.

    abstract：:Nonhomologous end joining (NHEJ) and homologous recombination (HR) represent the two major pathways of DNA double-strand break (DSB) repair in eukaryotic cells. NHEJ repairs DSBs by ligation of cognate broken ends irrespective of homologous flanking sequences, whereas HR repairs DSBs using an undamaged homologous temp...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.1204304

    authors： Mills KD,Ferguson DO,Essers J,Eckersdorff M,Kanaar R,Alt FW

    更新日期：2004-06-01 00:00:00

  • Constitutive mTORC1 activation by a herpesvirus Akt surrogate stimulates mRNA translation and viral replication.

    abstract：:All viruses require cellular ribosomes to translate their mRNAs. Viruses producing methyl-7 (m⁷) GTP-capped mRNAs, like Herpes Simplex Virus-1 (HSV-1), stimulate cap-dependent translation by activating mTORC1 to inhibit the translational repressor 4E-binding protein 1 (4E-BP1). Here, we establish that the HSV-1 kinase...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.1978310

    authors： Chuluunbaatar U,Roller R,Feldman ME,Brown S,Shokat KM,Mohr I

    更新日期：2010-12-01 00:00:00

  • Termination-altering amino acid substitutions in the beta' subunit of Escherichia coli RNA polymerase identify regions involved in RNA chain elongation.

    abstract：:To identify regions of the largest subunit of RNA polymerase that are potentially involved in transcript elongation and termination, we have characterized amino acid substitutions in the beta' subunit of Escherichia coli RNA polymerase that alter expression of reporter genes preceded by terminators in vivo. Terminatio...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.8.23.2913

    authors： Weilbaecher R,Hebron C,Feng G,Landick R

    更新日期：1994-12-01 00:00:00

  • Rhythmic control of AANAT translation by hnRNP Q in circadian melatonin production.

    abstract：:The circadian rhythm of pineal melatonin requires the nocturnal increment of serotonin N-acetyltransferase (arylalkylamine N-acetyltransferase [AANAT]) protein. To date, only limited information is available in the critical issue of how AANAT protein expression is up-regulated exclusively at night regardless of its sp...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.1519507

    authors： Kim TD,Woo KC,Cho S,Ha DC,Jang SK,Kim KT

    更新日期：2007-04-01 00:00:00

  • Developmental control of the G1 to S transition in Drosophila: cyclin Eis a limiting downstream target of E2F.

    abstract：:The E2F transcription factor is required for S phase in Drosophila. While it also triggers expression of replication genes at the G1-S transition, the relevance of this transcription is not clear because many of the induced gene products are sufficiently stable that new expression is not required for S phase. However,...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.9.12.1456

    authors： Duronio RJ,O'Farrell PH

    更新日期：1995-06-15 00:00:00

  • A genetic screen identifies the Triple T complex required for DNA damage signaling and ATM and ATR stability.

    abstract：:In response to DNA damage, cells activate a complex signal transduction network called the DNA damage response (DDR). To enhance our current understanding of the DDR network, we performed a genome-wide RNAi screen to identify genes required for resistance to ionizing radiation (IR). Along with a number of known DDR ge...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.1934210

    authors： Hurov KE,Cotta-Ramusino C,Elledge SJ

    更新日期：2010-09-01 00:00:00

  • A novel role for the 3' region of introns in pre-mRNA splicing of Saccharomyces cerevisiae.

    abstract：:To investigate the importance of sequences between the yeast (Saccharomyces cerevisiae) branch point (TACTAAC box) and 3' splice site (AG), we generated a series of pre-mRNA substrates that differed in the length of RNA retained on the 3' side of the TACTAAC box. These pre-mRNAs were compared as substrates for the fir...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.1.3.238

    authors： Rymond BC,Torrey DD,Rosbash M

    更新日期：1987-05-01 00:00:00

  • Selective disruption of genes expressed in totipotent embryonal stem cells.

    abstract：:Two retrovirus promoter trap vectors (U3His and U3Neo) have been used to disrupt genes expressed in totipotent murine embryonal stem (ES) cells. Selection in L-histidinol or G418 produced clones in which the coding sequences for histidinol-dehydrogenase or neomycin-phosphotransferase were fused to sequences in or near...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.6.6.919

    authors： von Melchner H,DeGregori JV,Rayburn H,Reddy S,Friedel C,Ruley HE

    更新日期：1992-06-01 00:00:00

  • SMARCB1 loss interacts with neuronal differentiation state to block maturation and impact cell stability.

    abstract：:Atypical teratoid rhabdoid tumors (ATRTs) are challenging pediatric brain cancers that are predominantly associated with inactivation of the gene SMARCB1, a conserved subunit of the chromatin remodeling BAF complex, which has known contributions to developmental processes. To identify potential interactions between SM...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.339978.120

    authors： Parisian AD,Koga T,Miki S,Johann PD,Kool M,Crawford JR,Furnari FB

    更新日期：2020-10-01 00:00:00

  • Life on the edge: telomeres and persistent DNA breaks converge at the nuclear periphery.

    abstract：:Persistent DNA double-strand breaks and telomeres represent genomic hazards, as they can instigate inappropriate repair reactions. Two recent papers by Oza and colleagues (pp. 912-917) and Schober and colleagues (pp. 928-938) show that both types of DNA ends are sequestered from bulk DNA by Mps3, a SUN domain protein ...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.1805309

    authors： Gartenberg MR

    更新日期：2009-05-01 00:00:00

  • Ctr9, a key subunit of PAFc, affects global estrogen signaling and drives ERα-positive breast tumorigenesis.

    abstract：:The human RNA polymerase II (RNAPII)-associated factor complex (hPAFc) and its individual subunits have been implicated in human diseases, including cancer. However, its involvement in breast cancer awaits investigation. Using data mining and human breast cancer tissue microarrays, we found that Ctr9, the key scaffold...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.268722.115

    authors： Zeng H,Xu W

    更新日期：2015-10-15 00:00:00

  • A new mechanism controlling kinetochore-microtubule interactions revealed by comparison of two dynein-targeting components: SPDL-1 and the Rod/Zwilch/Zw10 complex.

    abstract：:Chromosome segregation requires stable bipolar attachments of spindle microtubules to kinetochores. The dynein/dynactin motor complex localizes transiently to kinetochores and is implicated in chromosome segregation, but its role remains poorly understood. Here, we use the Caenorhabditis elegans embryo to investigate ...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.1687508

    authors： Gassmann R,Essex A,Hu JS,Maddox PS,Motegi F,Sugimoto A,O'Rourke SM,Bowerman B,McLeod I,Yates JR 3rd,Oegema K,Cheeseman IM,Desai A

    更新日期：2008-09-01 00:00:00

  • Topoisomerases and yeast rRNA transcription: negative supercoiling stimulates initiation and topoisomerase activity is required for elongation.

    abstract：:Previous work has shown that rRNA synthesis is strongly inhibited in yeast top1-top2 double mutants. Here, we show that inactivation of yeast topoisomerases can have paradoxical effects on transcription by RNA polymerase I. For example, transcription of ribosomal minigenes on extrachromosomal plasmids is greatly stimu...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.6.7.1332

    authors： Schultz MC,Brill SJ,Ju Q,Sternglanz R,Reeder RH

    更新日期：1992-07-01 00:00:00

  • USP21 deubiquitinase promotes pancreas cancer cell stemness via Wnt pathway activation.

    abstract：:The ubiquitin-specific protease (USP) family is the largest group of cysteine proteases. Cancer genomic analysis identified frequent amplification of USP21 (22%) in human pancreatic ductal adenocarcinoma (PDAC). USP21 overexpression correlates with human PDAC progression, and enforced expression of USP21 accelerates m...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.326314.119

    authors： Hou P,Ma X,Zhang Q,Wu CJ,Liao W,Li J,Wang H,Zhao J,Zhou X,Guan C,Ackroyd J,Jiang S,Zhang J,Spring DJ,Wang YA,DePinho RA

    更新日期：2019-10-01 00:00:00

  • Phosphatidic acid mediates demyelination in Lpin1 mutant mice.

    abstract：:Lipids play crucial roles in many aspects of glial cell biology, affecting processes ranging from myelin membrane biosynthesis to axo-glial interactions. In order to study the role of lipid metabolism in myelinating glial cells, we specifically deleted in Schwann cells the Lpin1 gene, which encodes the Mg2+-dependent ...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.1638008

    authors： Nadra K,de Preux Charles AS,Médard JJ,Hendriks WT,Han GS,Grès S,Carman GM,Saulnier-Blache JS,Verheijen MH,Chrast R

    更新日期：2008-06-15 00:00:00

  • Dynamics of DNA damage response proteins at DNA breaks: a focus on protein modifications.

    abstract：:Genome integrity is constantly monitored by sophisticated cellular networks, collectively termed the DNA damage response (DDR). A common feature of DDR proteins is their mobilization in response to genotoxic stress. Here, we outline how the development of various complementary methodologies has provided valuable insig...

    journal_title：Genes & development

    pub_type： 杂志文章,评审

    doi：10.1101/gad.2021311

    authors： Polo SE,Jackson SP

    更新日期：2011-03-01 00:00:00

  • The HIV-1 Tat protein activates transcription from an upstream DNA-binding site: implications for Tat function.

    abstract：:The Tat protein of human immunodeficiency virus type 1 (HIV-1) activates transcription following binding to nascent trans-activation response (TAR) RNA downstream of the transcription start site. Because Tat functions when bound to RNA, and in a position-dependent manner, it has been proposed that Tat works by a novel...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.5.12b.2496

    authors： Southgate CD,Green MR

    更新日期：1991-12-01 00:00:00

  • Tango7 directs cellular remodeling by the Drosophila apoptosome.

    abstract：:It is now well appreciated that the apoptosome, which governs caspase-dependent cell death, also drives nonapoptotic caspase activation to remodel cells. However, the determinants that specify whether the apoptosome acts to kill or remodel have yet to be identified. Here we report that Tango7 collaborates with the Dro...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.219287.113

    authors： D'Brot A,Chen P,Vaishnav M,Yuan S,Akey CW,Abrams JM

    更新日期：2013-08-01 00:00:00

  • Localized surface activity of torso, a receptor tyrosine kinase, specifies terminal body pattern in Drosophila.

    abstract：:The subdivision of the Drosophila body into distinct terminal and central domains depends on the torso (tor) protein, a putative receptor tyrosine kinase that is active at both ends of the early embryo. We show that the tor protein is uniformly expressed along the surface membrane of early embryos despite its localize...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.3.12b.2025

    authors： Casanova J,Struhl G

    更新日期：1989-12-01 00:00:00

  • A six-amino-acid motif is a major determinant in functional evolution of HOX1 proteins.

    abstract：:Gene duplication and divergence is a major driver in the emergence of evolutionary novelties. How variations in amino acid sequences lead to loss of ancestral activity and functional diversification of proteins is poorly understood. We used cross-species functional analysis of Drosophila Labial and its mouse HOX1 orth...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.342329.120

    authors： Singh NP,De Kumar B,Paulson A,Parrish ME,Zhang Y,Florens L,Conaway JW,Si K,Krumlauf R

    更新日期：2020-12-01 00:00:00

  • pRb and condensin--local control of global chromosome structure.

    abstract：:Rb mutants exhibit aneuploidy and aberrant chromosome structure during mitosis. In this issue of Genes & Development, a new paper from Longworth and colleagues (1011-1024) describes both physical and functional interactions between Drosophila Rbf1 and the dCAP-D3 subunit of condensin II. This work directly implicates ...

    journal_title：Genes & development

    pub_type： 评论,杂志文章

    doi：10.1101/gad.1666808

    authors： Lavoie BD

    更新日期：2008-04-15 00:00:00

  • The function of Xenopus Bloom's syndrome protein homolog (xBLM) in DNA replication.

    abstract：:The Bloom's syndrome gene (BLM) plays a pivotal role in the maintenance of genomic stability in somatic cells. It encodes a DNA helicase (BLM) of the RecQ family, but the exact function of BLM remains elusive. To study this question, we have cloned the BLM homolog of the frog Xenopus laevis (xBLM) and have raised anti...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.822400

    authors： Liao S,Graham J,Yan H

    更新日期：2000-10-15 00:00:00

  • Reelin-induced tyrosine [corrected] phosphorylation of disabled 1 during neuronal positioning.

    abstract：:The reelin (reln) and disabled 1 (dab1) genes both ensure correct neuronal positioning during brain development. We have found that the intracellular Dab1 protein receives a tyrosine phosphorylation signal from extracellular Reln protein. Genetic analysis shows that reln function depends on dab1, and vice versa, as ex...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.13.6.643

    authors： Howell BW,Herrick TM,Cooper JA

    更新日期：1999-03-15 00:00:00

  • Activator recruitment by the general transcription machinery: X-ray structural analysis of the Oct-1 POU domain/human U1 octamer/SNAP190 peptide ternary complex.

    abstract：:Transcriptional activation of the human U1 snRNA genes is dependent on a noncanonical octamer element contained within an upstream enhancer. The U1 octamer only weakly recruits the Oct-1 POU domain, although recruitment is stimulated by a peptide containing the Oct-1-binding domain of SNAP190. Structural analysis of t...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.1021002

    authors： Hovde S,Hinkley CS,Strong K,Brooks A,Gu L,Henry RW,Geiger J

    更新日期：2002-11-01 00:00:00