Abstract：
:Alteration of nucleosomes by ATP-dependent remodeling complexes represents a critical step in the regulation of transcription. The human SWI/SNF (hSWI/SNF) family is composed of complexes that contain either Brg1 or hBrm as the central ATPase; however, these separate complexes have not been compared functionally. Here we describe the establishment of cell lines that express epitope-tagged Brg1 and hBrm and a characterization of the complexes associated with these two ATPases. We show that Brg1 fractionates into two complexes that differ in activity and subunit composition, whereas hBrm is found in one complex with lower activity than the Brg1 complexes. These three complexes can remodel nucleosomal arrays, increase restriction enzyme accessibility, and hydrolyze ATP in a DNA-dependent manner. The three complexes differ markedly in their ability to remodel mononucleosomal core particles. We also show that the hBrm complex and one of the Brg1 complexes contain components of the mammalian Sin3 (mSin3) complex. In addition, we have found that Brg1, hBrm, and BAF155 can interact specifically with mSin3A in vitro, showing a direct association of hSWI/SNF complexes with proteins involved in gene repression. These unexpected functional characteristics indicate that these hSWI/SNF complexes play diverse regulatory roles.
journal_name
Genes Devjournal_title
Genes & developmentauthors
Sif S,Saurin AJ,Imbalzano AN,Kingston REdoi
10.1101/gad.872801subject
Has Abstractpub_date
2001-03-01 00:00:00pages
603-18issue
5eissn
0890-9369issn
1549-5477journal_volume
15pub_type
杂志文章abstract：:Erasure and subsequent reinstatement of DNA methylation in the germline, especially at imprinted CpG islands (CGIs), is crucial to embryogenesis in mammals. The mechanisms underlying DNA methylation establishment remain poorly understood, but a number of post-translational modifications of histones are implicated in a...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.271353.115
更新日期：2015-12-01 00:00:00
abstract：:The repression of the maternally inherited Igf2 allele has been proposed to depend on a methylation-sensitive chromatin insulator organized by the 11 zinc finger protein CTCF at the H19 imprinting control region (ICR). Here we document that point mutations of the nucleotides in physical contact with CTCF within the en...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.254903
更新日期：2003-03-01 00:00:00
abstract：:To investigate functional differences between RNA polymerases IIA and IIO (Pol IIA and Pol IIO), with hypo- and hyperphosphorylated carboxy-terminal repeat domains (CTDs), respectively, we have visualized the in vivo distributions of the differentially phosphorylated forms of Pol II on Drosophila polytene chromosomes....
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.7.12a.2329
更新日期：1993-12-01 00:00:00
abstract：:Oligophrenin-1 (OPHN1) encodes a Rho-GTPase-activating protein (Rho-GAP) whose loss of function has been associated with X-linked mental retardation (MR). The pathophysiological role of OPHN1, however, remains poorly understood. Here we show that OPHN1 through its Rho-GAP activity plays a critical role in the activity...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1783809
更新日期：2009-06-01 00:00:00
abstract：:Recruitment of p300/CBP by the hypoxia-inducible factor, HIF-1, is essential for the transcriptional response to hypoxia and requires an interaction between the p300/CBP CH1 region and HIF-1alpha. A new p300-CH1 interacting protein, p35srj, has been identified and cloned. p35srj is an alternatively spliced isoform of ...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.13.1.64
更新日期：1999-01-01 00:00:00
abstract：:AAUAAA is the most highly conserved motif in eukaryotic mRNA polyadenylation sites and, in mammals, is specifically recognized by the multisubunit CPSF (cleavage and polyadenylation specificity factor) complex. Despite its critical functions in mRNA 3' end formation, the molecular basis for CPSF-AAUAAA interaction rem...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.250993.114
更新日期：2014-11-01 00:00:00
abstract：:SpoIIIE is an FtsK-related protein that transports the forespore chromosome across the Bacillus subtilis sporulation septum. We use membrane photobleaching and protoplast assays to demonstrate that SpoIIIE is required for septal membrane fission in the presence of trapped DNA, and that DNA is transported across separa...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1925210
更新日期：2010-06-01 00:00:00
abstract：:Post-translational processes are essential for the generation and dynamics of mammalian circadian rhythms. In particular, phosphorylation of the key circadian protein PER2 precisely controls the period and phase of circadian oscillations. However, the mechanisms underlying that control are poorly understood. Here, we ...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.512209
更新日期：2009-03-15 00:00:00
abstract：:The ATR (ATM and Rad3-related) kinase and its regulatory partner ATRIP (ATR-interacting protein) coordinate checkpoint responses to DNA damage and replication stress. TopBP1 functions as a general activator of ATR. However, the mechanism by which TopBP1 activates ATR is unknown. Here, we show that ATRIP contains a Top...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1666208
更新日期：2008-06-01 00:00:00
abstract：:To determine whether 9-cis retinoic acid receptors (RXRs) regulate the biological activity of all-trans retinoic acid (tRA) and its receptors (RARs) in skin, we have targeted a dominant-negative RXR alpha (dnRXR alpha) lacking transactivation function AF-2 to differentiated suprabasal keratinocytes in the epidermis of...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.11.1.59
更新日期：1997-01-01 00:00:00
abstract：:Sequence-specific DNA-binding activators, key regulators of gene expression, stimulate transcription in part by targeting the core promoter recognition TFIID complex and aiding in its recruitment to promoter DNA. Although it has been established that activators can interact with multiple components of TFIID, it is unk...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1790709
更新日期：2009-07-01 00:00:00
abstract：:Mesoderm induction assays in Xenopus have implicated growth factors such as activin, Vg1, Xwnt-8, and noggin as important in directing the formation of dorsal mesoderm (Spemann's organizer). Because these growth factors are structurally very different, they presumably act through distinct cell surface receptors that i...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.9.24.3038
更新日期：1995-12-15 00:00:00
abstract：:During vasculogenesis and angiogenesis, endothelial cell responses to growth factors are modulated by the compositional and mechanical properties of a surrounding three-dimensional (3D) extracellular matrix (ECM) that is dominated by either cross-linked fibrin or type I collagen. While 3D-embedded endothelial cells es...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1643308
更新日期：2008-05-01 00:00:00
abstract：:U6 small nuclear RNA is one of the spliceosomal RNAs involved in pre-mRNA splicing. In the fission yeast Schizosaccharomyces pombe, the U6 RNA gene was found to have an intron similar to a nuclear pre-mRNA intron, and it was proposed that the U6 intron might be inserted erroneously during pre-mRNA splicing. Using the ...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.5.6.1022
更新日期：1991-06-01 00:00:00
abstract：:The mammalian high mobility group proteins HMG1 and HMG2 are abundant, chromatin-associated proteins whose cellular function is not known. In this study we show that these proteins can substitute for the prokaryotic DNA-bending protein HU in promoting the assembly of the Hin invertasome, an intermediate structure in H...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.7.8.1521
更新日期：1993-08-01 00:00:00
abstract：:The molecular mechanisms driving brain development at risk in autism spectrum disorders (ASDs) remain mostly unknown. Previous studies have implicated the transcription factor FOXP1 in both brain development and ASD pathophysiology. However, the specific molecular pathways both upstream of and downstream from FOXP1 ar...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.305037.117
更新日期：2017-10-15 00:00:00
abstract：:The transcription factor NF-kappaB is a critical regulator of inflammatory and cell survival signals. Proteasomal degradation of NF-kappaB subunits plays an important role in the termination of NF-kappaB activity, and at least one of the identified ubiquitin ligases is a multimeric complex containing Copper Metabolism...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1748409
更新日期：2009-04-01 00:00:00
abstract：:An insulin receptor-like signaling pathway regulates Caenorhabditis elegans metabolism, development, and longevity. Inactivation of the insulin receptor homolog DAF-2, the AGE-1 PI3K, or the AKT-1 and AKT-2 kinases causes a developmental arrest at the dauer stage. A null mutation in the daf-16 Fork head transcription ...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.13.11.1438
更新日期：1999-06-01 00:00:00
abstract：:A conditional block to transcriptional elongation is an important mechanism for regulating c-myc gene expression. This elongation block within the first c-myc exon was defined originally in mammalian cells by nuclear run-on transcription analyses. Subsequent oocyte injection and in vitro transcription analyses suggest...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.6.11.2201
更新日期：1992-11-01 00:00:00
abstract：:The recombination-activating gene 1 (RAG1) and RAG2 proteins initiate V(D)J recombination, the process that assembles the B- and T-lymphocyte antigen receptor genes of jawed vertebrates. RAG1 and RAG2 are thought to have arisen from a transposable element, but the origins of this element are not understood. We show th...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.278432.116
更新日期：2016-04-15 00:00:00
abstract：:We have investigated the role of erythroid Kruppel-like factor (EKLF) in expression of the human beta-globin genes in compound EKLF knockout/human beta-locus transgenic mice. EKLF affects only the adult mouse beta-globin genes in homozygous knockout mice; heterozygous mice are unaffected. Here we show that EKLF knocko...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.10.22.2894
更新日期：1996-11-15 00:00:00
abstract：:In this issue of Genes & Development, Wierzbicki and colleagues (pp. 1825-1836) examine the current model of RNA-directed DNA methylation (RdDM) by determining genome-wide distributions of RNA polymerase V (Pol V) occupancy, siRNAs, and DNA methylation. Their data support the key role of base-pairing between Pol V tra...
journal_title：Genes & development
pub_type： 评论,杂志文章
doi：10.1101/gad.200410.112
更新日期：2012-08-15 00:00:00
abstract：:The discovery of transcription factors (TFs) controlling pathways in health and disease is of paramount interest. We designed a widely applicable method, dubbed barcorded synthetic tandem repeat promoter screening (BC-STAR-PROM), to identify signal-activated TFs without any a priori knowledge about their properties. T...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.284828.116
更新日期：2016-08-15 00:00:00
abstract：:We have used the 100-kD HeLa cell octamer-binding protein OBP100 as a model to study flexible DNA sequence recognition by promoter-binding proteins. OBP100 binds to the conserved octamer motif ATGCAAAT found in numerous promoters and additionally to two degenerate octamer motifs (sites I and II) within the SV40 enhanc...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.2.11.1400
更新日期：1988-11-01 00:00:00
abstract：:Faithful inheritance of genetic information requires that DNA be copied only once each cell cycle. Initiation of DNA replication involves the establishment of a prereplication complex (pre-RC) and subsequent activation by CDK/cyclins, converting the pre-RC to a post-RC. The origin recognition complex (ORC), Cdc6p, and...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.11.24.3375
更新日期：1997-12-15 00:00:00
abstract：:Infection of metazoan cells with some viruses alters the balance of cellular mRNA export to favor viral RNA export and to retain cellular transcripts in the nucleus. Here, evidence is presented to show that the herpes simplex virus 1 (HSV-1) essential regulatory protein ICP27, which inhibits host cell-splicing, result...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.12.6.868
更新日期：1998-03-15 00:00:00
abstract：:The activity of the polyoma virus (PyV) origin of DNA replication was used as a sensitive assay for enhancer function in one- and two-cell mouse embryos by injecting embryos with plasmid DNA containing different PyV ori configurations, allowing them to continue development in vitro, and then measuring plasmid DNA repl...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.2.9.1115
更新日期：1988-09-01 00:00:00
abstract：:The oncoprotein MDM2 binds to the activation domain of the tumor suppressor p53 and inhibits its ability to stimulate transcription. This same region of p53 is able to bind several basal transcription factors that appear to be important for the transactivation function of p53. It has therefore been suggested that MDM2...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.11.15.1974
更新日期：1997-08-01 00:00:00
abstract：:The gene for an essential protein subunit of nuclear RNase P from Saccharomyces cerevisiae has been cloned. The gene for this protein, RPP1, was identified by virtue of its homology with a human scleroderma autoimmune antigen, Rpp30, which copurifies with human RNase P. Epitope-tagged Rpp1 can be found in association ...
journal_title：Genes & development
pub_type： 更正并重新发布的文章,杂志文章
doi：10.1101/gad.11.21.2926
更新日期：1997-11-01 00:00:00
abstract：:Disruption of the balanced modulation of reversible tyrosine phosphorylation has been implicated in the etiology of various human cancers, including breast cancer. Protein Tyrosine Phosphatase N23 (PTPN23) resides in chromosomal region 3p21.3, which is hemizygously or homozygously lost in some breast cancer patients. ...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.304261.117
更新日期：2017-10-01 00:00:00
