Abstract:
:A conditional block to transcriptional elongation is an important mechanism for regulating c-myc gene expression. This elongation block within the first c-myc exon was defined originally in mammalian cells by nuclear run-on transcription analyses. Subsequent oocyte injection and in vitro transcription analyses suggested that sequences near the end of the first c-myc exon are sites of attenuation and/or premature termination. We report here that the mapping of single stranded DNA in vivo with potassium permanganate (KMnO4) and nuclear run-on transcription assays reveal that polymerase is paused near position +30 relative to the major c-myc transcription initiation site. Deletion of 350 bp, including the sites of 3'-end formation and intrinsic termination defined in oocyte injection and in vitro transcription assays does not affect-the pausing of polymerase in the promoter-proximal region. In addition, sequences upstream of +47 are sufficient to confer the promoter-proximal pausing of polymerases and to generate the polarity of transcription farther downstream. Thus, the promoter-proximal pausing of RNA polymerase II complexes accounts for the block to elongation within the c-myc gene in mammalian cells. We speculate that modification of polymerase complexes at the promoter-proximal pause site may determine whether polymerases can read through intrinsic sites of termination farther downstream.
journal_name
Genes Devjournal_title
Genes & developmentauthors
Krumm A,Meulia T,Brunvand M,Groudine Mdoi
10.1101/gad.6.11.2201subject
Has Abstractpub_date
1992-11-01 00:00:00pages
2201-13issue
11eissn
0890-9369issn
1549-5477journal_volume
6pub_type
杂志文章abstract::3' end formation of pre-mRNAs is coupled to their transcription via the C-terminal domain (CTD) of RNA polymerase II (Pol II). Nearly all protein-coding transcripts are matured by cleavage and polyadenylation (CPA), which is frequently misregulated in disease. Understanding how transcription is coordinated with CPA in...
journal_title:Genes & development
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journal_title:Genes & development
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journal_title:Genes & development
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journal_title:Genes & development
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journal_title:Genes & development
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journal_title:Genes & development
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journal_title:Genes & development
pub_type: 杂志文章
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更新日期:2005-08-15 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.1252404
更新日期:2004-12-15 00:00:00
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journal_title:Genes & development
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更新日期:2011-01-15 00:00:00
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journal_title:Genes & development
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abstract::Overexpression of the type II transmembrane serine protease matriptase is a highly consistent feature of human epithelial tumors. Here we show that matriptase possesses a strong oncogenic potential when unopposed by its endogenous inhibitor, HAI-1. Modest orthotopic overexpression of matriptase in the skin of transgen...
journal_title:Genes & development
pub_type: 杂志文章
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abstract::Expression of the Arabidopsis CGS1 gene that codes for cystathionine gamma-synthase is feedback regulated at the step of mRNA stability in response to S-adenosyl-L-methionine (AdoMet). A short stretch of amino acid sequence, called the MTO1 region, encoded by the first exon of CGS1 itself is involved in this regulatio...
journal_title:Genes & development
pub_type: 杂志文章
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更新日期:2005-08-01 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.13.16.2134
更新日期:1999-08-15 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.5.5.728
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pub_type: 杂志文章,评审
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journal_title:Genes & development
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journal_title:Genes & development
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更新日期:2007-10-01 00:00:00
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journal_title:Genes & development
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更新日期:2000-06-15 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
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更新日期:1991-12-01 00:00:00
abstract::H4K20 methylation is a broad chromatin modification that has been linked with diverse epigenetic functions. Several enzymes target H4K20 methylation, consistent with distinct mono-, di-, and trimethylation states controlling different biological outputs. To analyze the roles of H4K20 methylation states, we generated c...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.476008
更新日期:2008-08-01 00:00:00
abstract::The acetylation state of histones plays a central role in determining gene expression in chromatin. The reestablishment of the acetylation state of nucleosomes after DNA replication and chromatin assembly requires both deacetylation and acetylation of specific lysine residues on newly incorporated histones. In this st...
journal_title:Genes & development
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更新日期:2001-12-01 00:00:00
abstract::Telomerase is a ribonucleoprotein enzyme that adds telomeric repeats to chromosomes, maintaining telomere length and stabilizing chromosome ends. In vitro, telomerase from the ciliate Tetrahymena elongates single-stranded, guanosine-rich DNA primers by adding repeats of the Tetrahymena telomeric sequence, dT2G4. We ha...
journal_title:Genes & development
pub_type: 杂志文章
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更新日期:1993-07-01 00:00:00
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journal_title:Genes & development
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更新日期:2013-07-15 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
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journal_title:Genes & development
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更新日期:2018-03-01 00:00:00
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journal_title:Genes & development
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更新日期:2002-12-01 00:00:00