Abstract:
:The abundance of retinoic acid (RA) is determined by the balance between its synthesis by retinaldehyde dehydrogenase (RALDH) and its degradation by CYP26. In particular, the dynamic expression of three CYP26 genes controls the regional level of RA within the body. Pregastrulation mouse embryos express CYP26 but not RALDH. We now show that mice lacking all three CYP26 genes manifest duplication of the body axis as a result of expansion of the Nodal expression domain throughout the epiblast. Mouse Nodal was found to contain an RA-responsive element in intron 1 that is highly conserved among mammals. In the absence of CYP26, maternally derived RA activates Nodal expression in the entire epiblast of pregastrulation embryos via this element. These observations suggest that maternal RA must be removed by embryonic CYP26 for correct Nodal expression during embryonic patterning.
journal_name
Genes Devjournal_title
Genes & developmentauthors
Uehara M,Yashiro K,Takaoka K,Yamamoto M,Hamada Hdoi
10.1101/gad.1776209subject
Has Abstractpub_date
2009-07-15 00:00:00pages
1689-98issue
14eissn
0890-9369issn
1549-5477pii
23/14/1689journal_volume
23pub_type
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