Abstract:
:DNA loops that juxtapose the promoter and terminator regions of RNA polymerase II-transcribed genes have been identified in yeast and mammalian cells. Loop formation is transcription-dependent and requires components of the pre-mRNA 3'-end processing machinery. Here we report that looping at the yeast GAL10 gene persists following a cycle of transcriptional activation and repression. Moreover, GAL10 and a GAL1p-SEN1 reporter undergo rapid reactivation kinetics following a cycle of activation and repression-a phenomenon defined as "transcriptional memory"-and this effect correlates with the persistence of looping. We propose that gene loops facilitate transcriptional memory in yeast.
journal_name
Genes Devjournal_title
Genes & developmentauthors
Lainé JP,Singh BN,Krishnamurthy S,Hampsey Mdoi
10.1101/gad.1823609subject
Has Abstractpub_date
2009-11-15 00:00:00pages
2604-9issue
22eissn
0890-9369issn
1549-5477pii
23/22/2604journal_volume
23pub_type
杂志文章abstract::Chk2/hcds1, the human homolog of the Saccharomyces cerevisiae RAD53/SPK1 and Schizosaccharomyces pombe cds1 DNA damage checkpoint genes, encodes a protein kinase that is post-translationally modified after DNA damage. Like its yeast homologs, the Chk2/hCds1 protein phosphorylates Cdc25C in vitro, suggesting that it ar...
journal_title:Genes & development
pub_type: 杂志文章
doi:
更新日期:2000-02-01 00:00:00
abstract::The homeless (hls) gene of Drosophila is required for anteroposterior and dorsoventral axis formation during oogenesis. At a low frequency, females homozygous for mutations in hls generate early egg chambers in which the oocyte is positioned incorrectly within the cyst. At a high frequency, late-stage egg chambers exh...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.9.20.2495
更新日期:1995-10-15 00:00:00
abstract::In mitosis, the spindle checkpoint protein Mad2 averts aneuploidy by delaying anaphase onset until chromosomes align. Here we show that depletion of Mad2 in meiosis I mouse oocytes induced an increased incidence of aneuploidy. Proteolysis of cyclin B and securin commenced earlier in Mad2-depleted oocytes, resulting in...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.328105
更新日期:2005-01-15 00:00:00
abstract::The visual system is a powerful model for probing the development, connectivity, and function of neural circuits. Two genetically tractable species, mice and flies, are together providing a great deal of understanding of these processes. Current efforts focus on integrating knowledge gained from three cross-fostering ...
journal_title:Genes & development
pub_type: 杂志文章,评审
doi:10.1101/gad.248245.114
更新日期:2014-12-01 00:00:00
abstract::Checkpoint genes maintain genomic stability by arresting cells after DNA damage. Many of these genes also control cell cycle events in unperturbed cells. By conducting a screen for checkpoint genes in zebrafish, we found that dtl/cdt2 is an essential component of the early, radiation-induced G2/M checkpoint. We subseq...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.1482106
更新日期:2006-11-15 00:00:00
abstract::Most eukaryotic mRNAs are translated using a cap-dependent mechanism of translation. However, approximately 10% of mammalian mRNAs initiate translation using a cap-independent mechanism that is not well understood. These mRNAs contain an internal ribosome entry site (IRES) located in the 5' untranslated region. The cr...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.1832209
更新日期:2009-12-01 00:00:00
abstract::Protection of the stalled replication fork is crucial for responding to replication stress and minimizing its impact on chromosome instability, thus preventing diseases, including cancer. We found a new component, Abro1, in the protection of stalled replication fork integrity. Abro1 deficiency results in increased chr...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.299172.117
更新日期:2017-07-15 00:00:00
abstract::The unique property of stem cells to self-renew suggests specific mechanisms that regulate their cell-cycle progression. In the present study, we identify a novel protein, nucleostemin, found in the nucleoli of CNS stem cells, embryonic stem cells, and several cancer cell lines and preferentially expressed by other st...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.55671
更新日期:2002-12-01 00:00:00
abstract::We have identified protein kinase C-zeta (PKC-zeta) as a novel suppressor of neoplastic transformation caused by the v-raf oncogene. PKC-zeta overexpression drastically retards proliferation, abolishes anchorage-independent growth, and reverts the morphological transformation of v-raf-transformed NIH-3T3 cells. The mo...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.10.12.1455
更新日期:1996-06-15 00:00:00
abstract::Pcf11 is one of numerous proteins involved in pre-mRNA 3'-end processing and transcription termination. Using elongation complexes (ECs) formed from purified yeast RNA polymerase II (Pol II), we show that a 140-amino acid polypeptide from yeast Pcf11 is capable of dismantling the EC in vitro. This action depends on th...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.1296305
更新日期:2005-07-01 00:00:00
abstract::The Drosophila external sensory organ forms in a lineage elaborating from a single precursor cell via a stereotypical series of asymmetric divisions. HAMLET transcription factor expression demarcates the lineage branch that generates two internal cell types, the external sensory neuron and thecogen. In HAMLET mutant o...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.1170904
更新日期:2004-03-15 00:00:00
abstract::Maternal imprinting at the Xist gene is essential to achieve paternal allele-specific imprinted X-chromosome inactivation (XCI) in female mammals. However, the mechanism underlying Xist imprinting is unclear. Here we show that the Xist locus is coated with a broad H3K27me3 domain that is established during oocyte grow...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.304113.117
更新日期:2017-10-01 00:00:00
abstract::The proteasome is well known for its regulation of the cell cycle and degradation of mis-folded proteins, yet many of its functions are still unknown. We show that RPN11, a gene encoding a subunit of the regulatory cap of the proteasome, is required for UV-stimulated activation of Gcn4p target genes, but is dispensabl...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.863801
更新日期:2001-01-15 00:00:00
abstract::The timing of asexual fruiting body formation during Aspergillus nidulans colony development is precisely regulated so that conidiophores are typically produced 1-2 mm behind the growing edge of the colony. Mutations in any of four A. nidulans genes, flbB, flbC, flbD, or flbE, result in colonies that are delayed at le...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.9.4.491
更新日期:1995-02-15 00:00:00
abstract::Children with trisomy 21/Down syndrome (DS) are at high risk to develop acute megakaryoblastic leukemia (DS-AMKL) and the related transient leukemia (DS-TL). The factors on human chromosome 21 (Hsa21) that confer this predisposing effect, especially in synergy with consistently mutated transcription factor GATA1 (GATA...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.1856210
更新日期:2010-03-01 00:00:00
abstract::Changes in the pattern of gene expression play an important role in allowing cancer cells to acquire their hallmark characteristics, while genomic instability enables cells to acquire genetic alterations that promote oncogenesis. Chromatin plays central roles in both transcriptional regulation and the maintenance of g...
journal_title:Genes & development
pub_type: 杂志文章,评审
doi:10.1101/gad.255182.114
更新日期:2015-02-01 00:00:00
abstract::Pseudoplasmodia of developing Dictyostelium are organized with anteroposterior polarity. We have isolated CAR4, the gene for a new cell-surface, G protein-linked cAMP receptor. CAR4 mRNA is initially expressed during tip elongation and continues to accumulate into culmination. CAR4 is maximally expressed in pseudoplas...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.8.17.2086
更新日期:1994-09-01 00:00:00
abstract::The evolutionarily conserved checkpoint protein kinase, TOR (target of rapamycin), has emerged as a major effector of cell growth and proliferation via the regulation of protein synthesis. Work in the last decade clearly demonstrates that TOR controls protein synthesis through a stunning number of downstream targets. ...
journal_title:Genes & development
pub_type: 杂志文章,评审
doi:10.1101/gad.1212704
更新日期:2004-08-15 00:00:00
abstract::Cosuppression is a silencing phenomenon triggered by the introduction of homologous DNA sequences into the genomes of organisms as diverse as plants, fungi, flies, and nematodes. Here we report sex-induced silencing (SIS), which is triggered by tandem integration of a transgene array in the human fungal pathogen Crypt...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.1970910
更新日期:2010-11-15 00:00:00
abstract::As in many polarized cells, spindle alignment in yeast is essential and cell cycle regulated. A key step that governs spindle alignment is the selective binding of the Kar9 protein to only one of the two spindle pole bodies (SPBs). It has been suggested that cyclin-dependent kinase Cdc28, in complex with cyclin Clb4, ...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.298704
更新日期:2004-07-15 00:00:00
abstract::The cytochrome P450 (CYP) gene products such as CYP3A and CYP2B are essential for the metabolism of steroid hormones and xenochemicals including prescription drugs. Nuclear receptor SXR/PXR (steroid and xenobiotic receptor/pregnenolone X receptor) has been shown both biochemically and genetically to activate CYP3A gen...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.846800
更新日期:2000-12-01 00:00:00
abstract::The release of Agrin by motoneurons activates the muscle-specific receptor tyrosine kinase (MuSK) as the main organizer of subsynaptic specializations at the neuromuscular junction. MuSK downstream signaling is largely undefined. Here we show that protein kinase CK2 interacts and colocalizes with MuSK at post-synaptic...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.375206
更新日期:2006-07-01 00:00:00
abstract::The incorporation of histone H1 into chromatin during embryogenesis directs the specific repression of the Xenopus oocyte 5S rRNA genes. An increase in histone H1 content specifically restricts TFIIIA-activated transcription, and a decrease in histone H1 within chromatin facilitates the activation of the oocyte 5S rRN...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.8.10.1147
更新日期:1994-05-15 00:00:00
abstract::Male sexual development in the nematode Caenorhabditis elegans requires the genes fem-1, fem-2, and fem-3. The current model of sex determination portrays the FEM proteins as components of a novel signal transduction pathway, but the mechanisms involved in signaling through the pathway are not understood. We report th...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.10.18.2314
更新日期:1996-09-15 00:00:00
abstract::Estrogen receptor α (ER) is the major driver of ∼75% of breast cancers, and multiple ER targeting drugs are routinely used clinically to treat patients with ER+ breast cancer. However, many patients relapse on these targeted therapies and ultimately develop metastatic and incurable disease, and understanding the mecha...
journal_title:Genes & development
pub_type: 杂志文章,评审
doi:10.1101/gad.316646.118
更新日期:2018-09-01 00:00:00
abstract::The subdivision of the Drosophila body into distinct terminal and central domains depends on the torso (tor) protein, a putative receptor tyrosine kinase that is active at both ends of the early embryo. We show that the tor protein is uniformly expressed along the surface membrane of early embryos despite its localize...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.3.12b.2025
更新日期:1989-12-01 00:00:00
abstract::In somatic cells of Xenopus, transcription of the TFIIIA gene initiates greater than 200 bp upstream from the start site used in oocytes. The resultant mRNA encodes a protein, S-TFIIIA, that is 22 amino acids longer at its amino terminus than the abundant form of TFIIIA in oocytes (O-TFIIIA). S-TFIIIA binds the 5S RNA...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.4.9.1602
更新日期:1990-09-01 00:00:00
abstract::MicroRNAs (miRNAs) and transcription factors (TFs) are primary metazoan gene regulators. Whereas much attention has focused on finding the targets of both miRNAs and TFs, the transcriptional networks that regulate miRNA expression remain largely unexplored. Here, we present the first genome-scale Caenorhabditis elegan...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.1678608
更新日期:2008-09-15 00:00:00
abstract::The discovery of transcription factors (TFs) controlling pathways in health and disease is of paramount interest. We designed a widely applicable method, dubbed barcorded synthetic tandem repeat promoter screening (BC-STAR-PROM), to identify signal-activated TFs without any a priori knowledge about their properties. T...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.284828.116
更新日期:2016-08-15 00:00:00
abstract::The histone variants H3.3 and H2A.Z have recently emerged as two of the most important features in transcriptional regulation, the molecular mechanism of which still remains poorly understood. In this study, we investigated the regulation of H3.3 and H2A.Z on chromatin dynamics during transcriptional activation. Our i...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.222174.113
更新日期:2013-10-01 00:00:00