Abstract：
:As in many polarized cells, spindle alignment in yeast is essential and cell cycle regulated. A key step that governs spindle alignment is the selective binding of the Kar9 protein to only one of the two spindle pole bodies (SPBs). It has been suggested that cyclin-dependent kinase Cdc28, in complex with cyclin Clb4, associates only with the SPB in the mother cell and so prevents Kar9 binding to this SPB. However, here we show that the nonoverexpressed Clb4 associates with the budward-directed SPB through Kar9. Cdc28-Clb4 then uses Kar9 as a carrier to move from this SPB to the plus ends of astral microtubules, where Cdc28-Clb4 regulates the interactions between microtubule ends and subdomains of the bud cortex. In the absence of Cdc28-Clb4 activity (G1/S phase), astral microtubules interact with the bud tip in a manner dependent on actin, Myo2, and Kar9. Coincidentally with reaching the bud cortex in S phase, Cdc28-Clb4 facilitates the dissociation of the microtubule bud tip interaction and their capture by the bud neck. This transition prevents the preanaphase spindle from becoming prematurely pulled into the bud. Thus, Cdc28-Clb4 facilitates spindle alignment by regulating the interaction of astral microtubules with subdomains of the bud cortex.
journal_name
Genes Devjournal_title
Genes & developmentauthors
Maekawa H,Schiebel Edoi
10.1101/gad.298704subject
Has Abstractpub_date
2004-07-15 00:00:00pages
1709-24issue
14eissn
0890-9369issn
1549-5477pii
18/14/1709journal_volume
18pub_type
杂志文章abstract：:The rpoH gene of Escherichia coli encodes sigma 32, the 32-kD sigma-factor responsible for the heat-inducible transcription of the heat shock genes. rpoH is transcribed from at least three promoters. Two of these promoters are recognized by RNA polymerase containing sigma 70, the predominant sigma-factor. We purified ...
journal_title：Genes & development
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journal_title：Genes & development
pub_type： 杂志文章
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更新日期：2005-02-15 00:00:00
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更新日期：2001-04-01 00:00:00
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pub_type： 杂志文章
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pub_type： 杂志文章
doi：10.1101/gad.204776.112
更新日期：2013-01-15 00:00:00
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journal_title：Genes & development
pub_type： 杂志文章
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更新日期：2003-07-15 00:00:00
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更新日期：1991-04-01 00:00:00
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journal_title：Genes & development
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更新日期：2008-02-01 00:00:00
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更新日期：1995-09-15 00:00:00
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更新日期：2014-07-15 00:00:00
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journal_title：Genes & development
pub_type： 评论,杂志文章
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更新日期：2013-06-15 00:00:00
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journal_title：Genes & development
pub_type： 杂志文章
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更新日期：2005-12-01 00:00:00
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journal_title：Genes & development
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更新日期：2020-03-01 00:00:00
