Abstract：
:The human T cell-specific transcription factor TCF-1 alpha plays a key role in the tissue-specific activation of the T cell receptor (TCR) C alpha enhancer and binds to pyrimidine-rich elements (5'-PyCTTTG-3') present in a variety of other T cell-specific control regions. Using amino acid sequence information derived from the DNA affinity-purified protein, we have now isolated cDNA clones encoding TCF-1 alpha. The TCF-1 alpha cDNA contains a single 68-amino-acid domain that is homologous to a region conserved among high-mobility group (HMG) and nonhistone chromosomal proteins. Expression of full-length and mutant cDNA clones in bacteria reveal that the single HMG motif, which is predicted to contain two extended alpha-helical segments, is sufficient to direct the sequence-specific binding of TCF-1 alpha to DNA. Northern blot experiments demonstrate further that TCF-1 alpha mRNA is highly tissue specific, found primarily in the thymus or T cell lines. The immature CEM T cell line expresses relatively low levels of TCF-1 alpha mRNA, which are increased upon activation of these cells by phorbol esters. Interestingly, the cloned TCF-1 alpha protein is a potent transcriptional activator of the human TCR alpha enhancer in nonlymphoid cell lines, whereas the activity of the endogenous protein in T cell lines is strongly dependent on an additional T cell-specific protein that interacts with the core enhancer. TCF-1 alpha is currently unique among the newly emerging family of DNA-binding regulatory proteins that share the HMG motif in that it is a highly tissue-specific RNA polymerase II transcription factor.
journal_name
Genes Devjournal_title
Genes & developmentauthors
Waterman ML,Fischer WH,Jones KAdoi
10.1101/gad.5.4.656subject
Has Abstractpub_date
1991-04-01 00:00:00pages
656-69issue
4eissn
0890-9369issn
1549-5477journal_volume
5pub_type
杂志文章abstract：:The mechanisms that determine the relative positions of floral organs, and thereby their numbers, is a poorly understood aspect of flower development. We isolated a petunia mutant, floozy (fzy), in which the formation of floral organ primordia in the outermost three floral whorls and one of the two bracts at the base ...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.219502
更新日期：2002-03-15 00:00:00
abstract：:Mammalian histone gene transcription is increased approximately fivefold during the transition from the G1 phase to the S phase of the cell cycle. In this study, we present a detailed in vivo analysis of the human histone H2b promoter, which establishes that transcriptional regulation of this gene is mediated by a sub...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.2.1.32
更新日期：1988-01-01 00:00:00
abstract：:MCM1 is an essential yeast DNA-binding protein that affects both minichromosome maintenance, in a manner suggesting that it has DNA replication initiation function, and gene expression. It activates alpha-specific genes together with MAT alpha 1, and represses a-specific genes together with MAT alpha 2. Alone, MCM1 ca...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.5.5.751
更新日期：1991-05-01 00:00:00
abstract：:The recombination-activating gene 1 (RAG1) and RAG2 proteins initiate V(D)J recombination, the process that assembles the B- and T-lymphocyte antigen receptor genes of jawed vertebrates. RAG1 and RAG2 are thought to have arisen from a transposable element, but the origins of this element are not understood. We show th...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.278432.116
更新日期：2016-04-15 00:00:00
abstract：:The rpoH gene of Escherichia coli encodes sigma 32, the 32-kD sigma-factor responsible for the heat-inducible transcription of the heat shock genes. rpoH is transcribed from at least three promoters. Two of these promoters are recognized by RNA polymerase containing sigma 70, the predominant sigma-factor. We purified ...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.3.9.1462
更新日期：1989-09-01 00:00:00
abstract：:We describe the developmental effects of the expression of a normal and mutated Drosophila ras gene ras2 in transgenic Drosophila. A Gly14----Val14 mutant (Gly14 in ras2 is equivalent to Gly12 in mammalian ras proteins) was constructed in ras2 by site-directed mutagenesis. Inducible constructs of ras2 and ras2Val14 we...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.2.5.567
更新日期：1988-05-01 00:00:00
abstract：:The 134-bp enhancer region of the pancreatic elastase I gene is sufficient to direct pancreatic acinar cell-specific transcription in transgenic mice and in transfected cells in culture. Ten-base-pair scanner mutations in three separate enhancer domains that inactivate enhancer function in transfected pancreatic cells...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.3.5.687
更新日期：1989-05-01 00:00:00
abstract：:To understand the organization of the transcriptional networks that govern cell differentiation, we have investigated the transcriptional circuitry controlling pseudohyphal development in Saccharomyces cerevisiae. The binding targets of Ste12, Tec1, Sok2, Phd1, Mga1, and Flo8 were globally mapped across the yeast geno...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1389306
更新日期：2006-02-15 00:00:00
abstract：:Maternal imprinting at the Xist gene is essential to achieve paternal allele-specific imprinted X-chromosome inactivation (XCI) in female mammals. However, the mechanism underlying Xist imprinting is unclear. Here we show that the Xist locus is coated with a broad H3K27me3 domain that is established during oocyte grow...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.304113.117
更新日期：2017-10-01 00:00:00
abstract：:Persistent DNA double-strand breaks and telomeres represent genomic hazards, as they can instigate inappropriate repair reactions. Two recent papers by Oza and colleagues (pp. 912-917) and Schober and colleagues (pp. 928-938) show that both types of DNA ends are sequestered from bulk DNA by Mps3, a SUN domain protein ...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1805309
更新日期：2009-05-01 00:00:00
abstract：:Our laboratory has been utilizing the Escherichia coli outer membrane protein LamB to study the mechanism of protein localization. Various lines of evidence suggest that, in addition to a signal sequence, regions within the mature protein are required for efficient localization. In particular, studies using LamB-LacZ ...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1.2.185
更新日期：1987-04-01 00:00:00
abstract：:To determine whether 9-cis retinoic acid receptors (RXRs) regulate the biological activity of all-trans retinoic acid (tRA) and its receptors (RARs) in skin, we have targeted a dominant-negative RXR alpha (dnRXR alpha) lacking transactivation function AF-2 to differentiated suprabasal keratinocytes in the epidermis of...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.11.1.59
更新日期：1997-01-01 00:00:00
abstract：:Structural cell-surface and extracellular-matrix proteins modulate intercellular signaling events during development, but how this is achieved remains largely unknown. Here we identify a novel family of Drosophila proteins, Nasrat and Polehole, that coat the oocyte surface and play two roles: They mediate assembly of ...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.223902
更新日期：2002-04-15 00:00:00
abstract：:The Swi/Snf chromatin remodeling complex functions to alter nucleosome positions by either sliding nucleosomes on DNA or the eviction of histones. The presence of histone acetylation and activator-dependent recruitment and retention of Swi/Snf is important for its efficient function. It is not understood, however, why...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.243584.114
更新日期：2014-10-15 00:00:00
abstract：:The p53 tumor suppressor protein is the most well studied as a regulator of transcription in the nucleus, where it exists primarily as a tetramer. However, there are other oligomeric states of p53 that are relevant to its regulation and activities. In unstressed cells, p53 is normally held in check by MDM2 that target...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.304071.117
更新日期：2018-03-01 00:00:00
abstract：:Evidence is presented that the activation of the RNA polymerase sigma factor sigma(W) in Bacillus subtilis by regulated intramembrane proteolysis is governed by a novel, membrane-embedded protease. The sigma(W) factor is activated by proteolytic destruction of the membrane-bound anti-sigma(W) factor RsiW in response t...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1440606
更新日期：2006-07-15 00:00:00
abstract：:Kinase suppressor of Ras (KSR) is a recently identified component of Ras-dependent signaling pathways. In this report, we show that murine KSR1 (mKSR1) cooperates with activated Ras to promote Xenopus oocyte maturation and cellular transformation and provide evidence that this cooperation occurs by accelerating mitoge...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.10.21.2684
更新日期：1996-11-01 00:00:00
abstract：:Gastrulation in mice is associated with the start of extreme proliferation and differentiation. The potential cost to the embryo of a very rapid proliferation rate is a high production of damaged cells. We demonstrate a novel surveillance mechanism for the elimination of cells damaged by ionizing radiation during mous...
journal_title：Genes & development
pub_type： 杂志文章
doi：
更新日期：2000-08-15 00:00:00
abstract：:Sonic hedgehog (Shh) signals via Gli transcription factors to direct digit number and identity in the vertebrate limb. We characterized the Gli-dependent cis-regulatory network through a combination of whole-genome chromatin immunoprecipitation (ChIP)-on-chip and transcriptional profiling of the developing mouse limb....
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1693008
更新日期：2008-10-01 00:00:00
abstract：:We report the discovery of mRNA 5'-leader trans-splicing (SL trans-splicing) in the chordates. In the ascidian protochordate Ciona intestinalis, the mRNAs of at least seven genes undergo trans-splicing of a 16-nucleotide 5'-leader apparently derived from a 46-nucleotide RNA that shares features with previously charact...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.865401
更新日期：2001-02-01 00:00:00
abstract：:We report on the function of the human ortholog of Saccharomyces cerevisiae Rif1 (Rap1-interacting factor 1). Yeast Rif1 associates with telomeres and regulates their length. In contrast, human Rif1 did not accumulate at functional telomeres, but localized to dysfunctional telomeres and to telomeric DNA clusters in AL...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1216004
更新日期：2004-09-01 00:00:00
abstract：:Spindle checkpoint silencing is a critical step during mitosis that initiates chromosome segregation, yet surprisingly little is known about its mechanism. Protein phosphatase I (PP1) was shown recently to be a key player in this process, and in this issue of Genes & Deverlopment, Akiyoshi and colleagues (pp. 2887-289...
journal_title：Genes & development
pub_type： 评论,杂志文章,评审
doi：10.1101/gad.1882109
更新日期：2009-12-15 00:00:00
abstract：:Transformation of primary rodent cells by the adenovirus E1A and E1B oncogenes is a two-step process, where E1A-dependent induction of proliferation is coupled to E1B-dependent suppression of programmed cell death (apoptosis). The E1B gene encodes two distinct transforming proteins, the 19K and 55K proteins, both of w...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.7.4.546
更新日期：1993-04-01 00:00:00
abstract：:One of the central requirements for eukaryotic chromosome stability is the maintenance of the simple sequence tracts at telomeres. In this study, we use genetic and physical assays to reveal the nature of a novel mechanism by which telomere length is controlled. This mechanism, telomeric rapid deletion (TRD), is capab...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.10.11.1310
更新日期：1996-06-01 00:00:00
abstract：:The Drosophila scratch (scrt) gene is expressed in most or all neuronal precursor cells and encodes a predicted zinc finger transcription factor closely related to the product of the mesoderm determination gene snail (sna). Adult flies homozygous for scrt null alleles have a reduced number of photoreceptors in the eye...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.9.19.2384
更新日期：1995-10-01 00:00:00
abstract：:The third-chromosome mutation Killer of prune (K-pn) causes no phenotype by itself, but causes lethality in individuals homozygous for the nonlethal X-chromosome mutation prune (pn). We have recovered 12 gamma-ray-induced revertants of Killer of prune. All of the revertants fail to complement a recessive cell lethal m...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.2.10.1333
更新日期：1988-10-01 00:00:00
abstract：:Neurofibromatosis type 1 (NF1) is a common neurodevelopmental disorder caused by impaired function of the neurofibromin RAS regulator. Using a combination of Nf1 genetically engineered mice and pharmacological/genetic inhibition approaches, we report that neurofibromin differentially controls neural stem cell (NSC) pr...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.261677.115
更新日期：2015-08-15 00:00:00
abstract：:Changes in the pattern of gene expression play an important role in allowing cancer cells to acquire their hallmark characteristics, while genomic instability enables cells to acquire genetic alterations that promote oncogenesis. Chromatin plays central roles in both transcriptional regulation and the maintenance of g...
journal_title：Genes & development
pub_type： 杂志文章,评审
doi：10.1101/gad.255182.114
更新日期：2015-02-01 00:00:00
abstract：:Keratinocyte growth factor (KGF), also known as fibroblast growth factor 7 (FGF7), is synthesized by skin fibroblasts. However, its mitogenic activity is on skin keratinocytes, where it is the most potent growth factor identified thus far. To explore KGF's function in vivo, we used embryonic stem cell technology to ge...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.10.2.165
更新日期：1996-01-15 00:00:00
abstract：:Experiments in Xenopus have illustrated the importance of extracellular morphogens for embryonic gene regulation in vertebrates. Much less is known about how induction leads to the correct positioning of boundaries; for example, between germ layers. Here we report that the neuroectoderm/mesoderm boundary is controlled...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.409507
更新日期：2007-04-15 00:00:00
