Overcoming inhibition in the spindle checkpoint.

abstract：:DNA methylation is a key regulator of embryonic stem cell (ESC) biology, dynamically changing between naïve, primed, and differentiated states. The p53 tumor suppressor is a pivotal guardian of genomic stability, but its contributions to epigenetic regulation and stem cell biology are less explored. We report that, in...

journal_title：Genes & development

authors： Tovy A,Spiro A,McCarthy R,Shipony Z,Aylon Y,Allton K,Ainbinder E,Furth N,Tanay A,Barton M,Oren M

更新日期：2017-05-15 00:00:00

abstract：:The reelin (reln) and disabled 1 (dab1) genes both ensure correct neuronal positioning during brain development. We have found that the intracellular Dab1 protein receives a tyrosine phosphorylation signal from extracellular Reln protein. Genetic analysis shows that reln function depends on dab1, and vice versa, as ex...

journal_title：Genes & development

authors： Howell BW,Herrick TM,Cooper JA

更新日期：1999-03-15 00:00:00

abstract：:A Drosophila FGF receptor homolog (DFGF-R2/DFRI) termed Heartless (Htl) is expressed in the embryonic mesoderm. The phenotypes of null mutant embryos demonstrated that Htl is a central player that is required for the development of several mesodermal lineages. No abnormalities in the primary specification of the mesod...

journal_title：Genes & development

authors： Beiman M,Shilo BZ,Volk T

更新日期：1996-12-01 00:00:00

abstract：:The orphan nuclear receptor TLX regulates neural stem cell self-renewal in the adult brain and functions primarily as a transcription repressor through recruitment of Atrophin corepressors, which bind to TLX via a conserved peptide motif termed the Atro box. Here we report crystal structures of the human and insect TL...

journal_title：Genes & development

authors： Zhi X,Zhou XE,He Y,Searose-Xu K,Zhang CL,Tsai CC,Melcher K,Xu HE

更新日期：2015-02-15 00:00:00

abstract：:The transcription factor TFIIB plays a central role in preinitiation complex assembly, providing a bridge between promoter-bound TFIID and RNA Polymerase II. TFIIB possesses sequence-specific DNA-binding ability and interacts with the TFIIB-recognition element (BRE), present in many promoters. Here we show that the BR...

journal_title：Genes & development

authors： Evans R,Fairley JA,Roberts SG

更新日期：2001-11-15 00:00:00

abstract：:U6 small nuclear RNA is one of the spliceosomal RNAs involved in pre-mRNA splicing. In the fission yeast Schizosaccharomyces pombe, the U6 RNA gene was found to have an intron similar to a nuclear pre-mRNA intron, and it was proposed that the U6 intron might be inserted erroneously during pre-mRNA splicing. Using the ...

journal_title：Genes & development

authors： Tani T,Ohshima Y

更新日期：1991-06-01 00:00:00

abstract：:Gene duplication and divergence is a major driver in the emergence of evolutionary novelties. How variations in amino acid sequences lead to loss of ancestral activity and functional diversification of proteins is poorly understood. We used cross-species functional analysis of Drosophila Labial and its mouse HOX1 orth...

journal_title：Genes & development

authors： Singh NP,De Kumar B,Paulson A,Parrish ME,Zhang Y,Florens L,Conaway JW,Si K,Krumlauf R

更新日期：2020-12-01 00:00:00

abstract：:The retinoblastoma tumor suppressor (pRb) protein associates with chromatin and regulates gene expression. Numerous studies have identified Rb-dependent RNA signatures, but the proteomic effects of Rb loss are largely unexplored. We acutely ablated Rb in adult mice and conducted a quantitative analysis of RNA and prot...

journal_title：Genes & development

authors： Nicolay BN,Danielian PS,Kottakis F,Lapek JD Jr,Sanidas I,Miles WO,Dehnad M,Tschöp K,Gierut JJ,Manning AL,Morris R,Haigis K,Bardeesy N,Lees JA,Haas W,Dyson NJ

更新日期：2015-09-01 00:00:00

abstract：:The WD40 domain-containing protein WRAP53β (WD40 encoding RNA antisense to p53; also referred to as WDR79/TCAB1) controls trafficking of splicing factors and the telomerase enzyme to Cajal bodies, and its functional loss has been linked to carcinogenesis, premature aging, and neurodegeneration. Here, we identify WRAP5...

journal_title：Genes & development

authors： Henriksson S,Rassoolzadeh H,Hedström E,Coucoravas C,Julner A,Goldstein M,Imreh G,Zhivotovsky B,Kastan MB,Helleday T,Farnebo M

更新日期：2014-12-15 00:00:00

abstract：:The POU transcription factor Oct-4 is expressed specifically in the germ line, pluripotent cells of the pregastrulation embryo and stem cell lines derived from the early embryo. Osteopontin (OPN) is a protein secreted by cells of the preimplantation embryo and contains a GRGDS motif that can bind to specific integrin ...

journal_title：Genes & development

authors： Botquin V,Hess H,Fuhrmann G,Anastassiadis C,Gross MK,Vriend G,Schöler HR

更新日期：1998-07-01 00:00:00

abstract：:In mitosis, the spindle checkpoint protein Mad2 averts aneuploidy by delaying anaphase onset until chromosomes align. Here we show that depletion of Mad2 in meiosis I mouse oocytes induced an increased incidence of aneuploidy. Proteolysis of cyclin B and securin commenced earlier in Mad2-depleted oocytes, resulting in...

journal_title：Genes & development

authors： Homer HA,McDougall A,Levasseur M,Yallop K,Murdoch AP,Herbert M

更新日期：2005-01-15 00:00:00

abstract：:Early endoderm formation in zebrafish requires at least three loci that function downstream of Nodal signaling but upstream of the early endodermal marker sox17: bonnie and clyde (bon), faust (fau), and casanova (cas). cas mutants show the most severe phenotype as they do not form any gut tissue and lack all sox17 exp...

journal_title：Genes & development

authors： Kikuchi Y,Agathon A,Alexander J,Thisse C,Waldron S,Yelon D,Thisse B,Stainier DY

更新日期：2001-06-15 00:00:00

abstract：:To find new genes that influence liver lipid mass, we performed a genetic screen for zebrafish mutants with hepatic steatosis, a pathological accumulation of fat. The red moon (rmn) mutant develops hepatic steatosis as maternally deposited yolk is depleted. Conversely, hepatic steatosis is suppressed in rmn mutants by...

journal_title：Genes & development

authors： Hugo SE,Cruz-Garcia L,Karanth S,Anderson RM,Stainier DY,Schlegel A

更新日期：2012-02-01 00:00:00

abstract：:The daughterless (da) gene is known to have separate maternal and zygotic functions: Maternally supplied daughterless activity is required for proper sex determination and dosage compensation in female embryos, whereas loss of zygotically supplied da+ activity causes embryonic lethality in both male and female embryos...

journal_title：Genes & development

authors： Caudy M,Grell EH,Dambly-Chaudière C,Ghysen A,Jan LY,Jan YN

更新日期：1988-07-01 00:00:00

abstract：:All transcription factors are equal, but some are more equal than others. In the 25 yr since the gene encoding the microphthalmia-associated transcription factor (MITF) was first isolated, MITF has emerged as a key coordinator of many aspects of melanocyte and melanoma biology. Like all transcription factors, MITF bin...

journal_title：Genes & development

authors： Goding CR,Arnheiter H

更新日期：2019-08-01 00:00:00

abstract：:During vasculogenesis and angiogenesis, endothelial cell responses to growth factors are modulated by the compositional and mechanical properties of a surrounding three-dimensional (3D) extracellular matrix (ECM) that is dominated by either cross-linked fibrin or type I collagen. While 3D-embedded endothelial cells es...

journal_title：Genes & development

authors： Zhou X,Rowe RG,Hiraoka N,George JP,Wirtz D,Mosher DF,Virtanen I,Chernousov MA,Weiss SJ

更新日期：2008-05-01 00:00:00

abstract：:The unique property of stem cells to self-renew suggests specific mechanisms that regulate their cell-cycle progression. In the present study, we identify a novel protein, nucleostemin, found in the nucleoli of CNS stem cells, embryonic stem cells, and several cancer cell lines and preferentially expressed by other st...

journal_title：Genes & development

authors： Tsai RY,McKay RD

更新日期：2002-12-01 00:00:00

abstract：:Start sites of DNA replication are marked by the origin recognition complex (ORC), which coordinates Mcm2-7 helicase loading to form the prereplicative complex (pre-RC). Although pre-RC assembly is well characterized in vitro, the process is poorly understood within the local chromatin environment surrounding replicat...

journal_title：Genes & development

authors： Belsky JA,MacAlpine HK,Lubelsky Y,Hartemink AJ,MacAlpine DM

更新日期：2015-01-15 00:00:00

abstract：:Transcription of frog rDNA by mouse cell factors is the only documented exception to the observed species selectivity of rRNA gene expression. This heterologous transcription is authentic in that it uses the normal frog upstream and core promoter domains, as well as the normal mouse polymerase I transcription factors,...

journal_title：Genes & development

authors： Pape LK,Windle JJ,Sollner-Webb B

更新日期：1990-01-01 00:00:00

abstract：:Motor axons make synaptic connections with specific muscles, and this specificity unfolds during development as motoneuron growth cones make specific pathway choices and ultimately recognize and synapse on their specific muscle targets. The Drosophila clueless mutation was identified previously in a genetic screen for...

journal_title：Genes & development

authors： Hu S,Fambrough D,Atashi JR,Goodman CS,Crews ST

更新日期：1995-12-01 00:00:00

abstract：:Bone morphogenic proteins 2 and 4 (BMP2 and BMP4) inhibit proliferation and induce differentiation of cerebellar granule neuron progenitors (GNPs) and primary GNP-like medulloblastoma (MB) cells. This occurs through rapid proteasome-mediated degradation of Math1 (Atoh1), a transcription factor expressed in proliferati...

journal_title：Genes & development

authors： Zhao H,Ayrault O,Zindy F,Kim JH,Roussel MF

更新日期：2008-03-15 00:00:00

abstract：:To understand how chromosome shapes are determined by actions of condensins and cohesin, we devised a series of protocols in which their levels are precisely changed in Xenopus egg extracts. When the relative ratio of condensin I to II is forced to be smaller, embryonic chromosomes become shorter and thicker, being re...

journal_title：Genes & development

authors： Shintomi K,Hirano T

更新日期：2011-07-15 00:00:00

abstract：:Chromatin reorganization is essential for transcriptional control by sequence-specific transcription factors. However, the molecular link between transcriptional control and chromatin reconfiguration remains unclear. By colocalization of the nuclear ecdysone receptor (EcR) on the ecdysone-induced puff in the salivary ...

journal_title：Genes & development

authors： Sawatsubashi S,Murata T,Lim J,Fujiki R,Ito S,Suzuki E,Tanabe M,Zhao Y,Kimura S,Fujiyama S,Ueda T,Umetsu D,Ito T,Takeyama K,Kato S

更新日期：2010-01-15 00:00:00

abstract：:The empty spiracles (ems) homeo box gene is required for the development of the Drosophila larval filzkörper, which are structural specializations of the eighth abdominal segment. Filzkörper development is also dependent on the function of the homeotic selector gene Abdominal-B (Abd-B). Here, we show that ems is a dow...

journal_title：Genes & development

authors： Jones B,McGinnis W

更新日期：1993-02-01 00:00:00

abstract：:All viruses require cellular ribosomes to translate their mRNAs. Viruses producing methyl-7 (m⁷) GTP-capped mRNAs, like Herpes Simplex Virus-1 (HSV-1), stimulate cap-dependent translation by activating mTORC1 to inhibit the translational repressor 4E-binding protein 1 (4E-BP1). Here, we establish that the HSV-1 kinase...

journal_title：Genes & development

authors： Chuluunbaatar U,Roller R,Feldman ME,Brown S,Shokat KM,Mohr I

更新日期：2010-12-01 00:00:00

abstract：:Reactive oxygen species (ROS) participate in numerous cell responses, including proliferation, DNA damage, and cell death. Based on these disparate activities, both promotion and inhibition of ROS have been proposed for cancer therapy. However, how the ROS response is determined is not clear. We examined the activitie...

journal_title：Genes & development

authors： Cheung EC,Lee P,Ceteci F,Nixon C,Blyth K,Sansom OJ,Vousden KH

更新日期：2016-01-01 00:00:00

abstract：:The cooperation of oncogenes in the transformation of primary rat Schwann cells is a strikingly synergistic process. We have explored the molecular mechanisms involved. Activation of an inducible Raf kinase results in morphologically transformed cells that are arrested in G1 via the induction of p21(CiP1) and subseque...

journal_title：Genes & development

authors： Lloyd AC,Obermüller F,Staddon S,Barth CF,McMahon M,Land H

更新日期：1997-03-01 00:00:00

abstract：:Noncoding RNA regulators have been implicated in almost all imaginable cellular processes. Here we review how regulatory small RNAs such as Spot42, SgrS, GlmY, and GlmZ and a cis-encoded ribozyme in glmS mRNA control sugar metabolism. Besides discussing the physiological implications, we show how the study of these mo...

journal_title：Genes & development

authors： Görke B,Vogel J

更新日期：2008-11-01 00:00:00

abstract：:H4K20 methylation is a broad chromatin modification that has been linked with diverse epigenetic functions. Several enzymes target H4K20 methylation, consistent with distinct mono-, di-, and trimethylation states controlling different biological outputs. To analyze the roles of H4K20 methylation states, we generated c...

journal_title：Genes & development

authors： Schotta G,Sengupta R,Kubicek S,Malin S,Kauer M,Callén E,Celeste A,Pagani M,Opravil S,De La Rosa-Velazquez IA,Espejo A,Bedford MT,Nussenzweig A,Busslinger M,Jenuwein T

更新日期：2008-08-01 00:00:00

abstract：:We isolated a novel bHLH protein gene Mesp2 (for mesoderm posterior 2) that cross-hybridizes with Mesp1 expressed in the early mouse mesoderm. Mesp2 is expressed in the rostral presomitic mesoderm, but down-regulated immediately after the formation of the segmented somites. To determine the function of MesP2 protein (...

journal_title：Genes & development

authors： Saga Y,Hata N,Koseki H,Taketo MM

更新日期：1997-07-15 00:00:00