Abstract：
:The POU transcription factor Oct-4 is expressed specifically in the germ line, pluripotent cells of the pregastrulation embryo and stem cell lines derived from the early embryo. Osteopontin (OPN) is a protein secreted by cells of the preimplantation embryo and contains a GRGDS motif that can bind to specific integrin subtypes and modulate cell adhesion/migration. We show that Oct-4 and OPN are coexpressed in the preimplantation mouse embryo and during differentiation of embryonal cell lines. Immunoprecipitation of the first intron of OPN (i-opn) from covalently fixed chromatin of embryonal stem cells by Oct-4-specific antibodies indicates that Oct-4 binds to this fragment in vivo. The i-opn fragment functions as an enhancer in cell lines that resemble cells of the preimplantation embryo. Furthermore, it contains a novel palindromic Oct factor recognition element (PORE) that is composed of an inverted pair of homeodomain-binding sites separated by exactly 5 bp (ATTTG +5 CAAAT). POU proteins can homo- and heterodimerize on the PORE in a configuration that has not been described previously. Strong transcriptional activation of the OPN element requires an intact PORE. In contrast, the canonical octamer overlapping with the downstream half of the PORE is not essential. Sox-2 is a transcription factor that contains an HMG box and is coexpressed with Oct-4 in the early mouse embryo. Sox-2 represses Oct-4 mediated activation of i-opn by way of a canonical Sox element that is located close to the PORE. Repression depends on a carboxy-terminal region of Sox-2 that is outside of the HMG box. Expression, DNA binding, and transactivation data are consistent with the hypothesis that OPN expression is regulated by Oct-4 and Sox-2 in preimplantation development.
journal_name
Genes Devjournal_title
Genes & developmentauthors
Botquin V,Hess H,Fuhrmann G,Anastassiadis C,Gross MK,Vriend G,Schöler HRdoi
10.1101/gad.12.13.2073subject
Has Abstractpub_date
1998-07-01 00:00:00pages
2073-90issue
13eissn
0890-9369issn
1549-5477journal_volume
12pub_type
杂志文章abstract：:Sox10 is a high-mobility-group transcriptional regulator in early neural crest. Without Sox10, no glia develop throughout the peripheral nervous system. Here we show that Sox10 is restricted in the central nervous system to myelin-forming oligodendroglia. In Sox10-deficient mice progenitors develop, but terminal diffe...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.215802
更新日期：2002-01-15 00:00:00
abstract：:Morphogenesis of the insect kidney, the Malpighian tubules, is controlled in Drosophila by a single large cell, the tip cell. It has been postulated that this cell sends out a mitogenic signal that induces the division of neighboring cells. The signal and the molecules that receive it have remained elusive. We show th...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.12.12.1781
更新日期：1998-06-15 00:00:00
abstract：:Bone metastases are the most common skeletal complication of malignancy. Tumor cells disrupt normal bone remodeling to promote bone destruction and its associated morbidity. In the August 15, 2009, issue of Genes & Development, Lu and colleagues (pp. 1882-1894) propose a novel molecular mechanism by which tumor-produc...
journal_title：Genes & development
pub_type： 评论,杂志文章
doi：10.1101/gad.1854909
更新日期：2009-09-15 00:00:00
abstract：:The Snail zinc-finger transcription factors trigger epithelial-mesenchymal transitions (EMTs), endowing epithelial cells with migratory and invasive properties during both embryonic development and tumor progression. During EMT, Snail provokes the loss of epithelial markers, as well as changes in cell shape and the ex...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.294104
更新日期：2004-05-15 00:00:00
abstract：:To determine the prevalence of cotranscriptional splicing in Drosophila, we sequenced nascent RNA transcripts from Drosophila S2 cells as well as from Drosophila heads. Eighty-seven percent of the introns assayed manifest >50% cotranscriptional splicing. The remaining 13% are cotranscriptionally spliced poorly or slow...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.178962.111
更新日期：2011-12-01 00:00:00
abstract：:The Dorsal morphogen is a transcription factor that activates some genes and represses others to establish multiple domains of gene expression along the dorsal/ventral axis of the early Drosophila embryo. Repression by Dorsal appears to require accessory proteins that bind to corepression elements in Dorsal-dependent ...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.9.24.3177
更新日期：1995-12-15 00:00:00
abstract：:Epigenetic modifications of chromatin play an important role in the regulation of gene expression. KMT4/Dot1 is a conserved histone methyltransferase capable of methylating chromatin on Lys79 of histone H3 (H3K79). Here we report the identification of a multisubunit Dot1 complex (DotCom), which includes several of the...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1898410
更新日期：2010-03-15 00:00:00
abstract：:Faithful inheritance of genetic information requires that DNA be copied only once each cell cycle. Initiation of DNA replication involves the establishment of a prereplication complex (pre-RC) and subsequent activation by CDK/cyclins, converting the pre-RC to a post-RC. The origin recognition complex (ORC), Cdc6p, and...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.11.24.3375
更新日期：1997-12-15 00:00:00
abstract：:The RNA polymerase II core promoter is a structurally and functionally diverse transcriptional module. RNAi depletion and overexpression experiments revealed a genetic circuit that controls the balance of transcription from two core promoter motifs, the TATA box and the downstream core promoter element (DPE). In this ...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1681808
更新日期：2008-09-01 00:00:00
abstract：:Translation of encephalomyocarditis virus (EMCV) mRNA occurs by ribosomal internal entry into the 5'-nontranslated region (5' NTR) rather than by ribosomal scanning. The internal ribosomal entry site (IRES) in the EMCV 5' NTR was determined by in vitro translation with RNAs that were generated by in vitro transcriptio...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.4.9.1560
更新日期：1990-09-01 00:00:00
abstract：:Telomerase is a ribonucleoprotein enzyme that adds telomeric repeats to chromosomes, maintaining telomere length and stabilizing chromosome ends. In vitro, telomerase from the ciliate Tetrahymena elongates single-stranded, guanosine-rich DNA primers by adding repeats of the Tetrahymena telomeric sequence, dT2G4. We ha...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.7.7b.1364
更新日期：1993-07-01 00:00:00
abstract：:The assembly and disassembly of protein complexes at replication origins play a crucial role in the regulation of chromosomal DNA replication. The sequential binding of the origin recognition complex (ORC), Cdc6, and the minichromosome maintenance (MCM/P1) proteins produces a licensed replication origin. Before the in...
journal_title：Genes & development
pub_type： 杂志文章
doi：
更新日期：2000-06-15 00:00:00
abstract：:The connection between RNA and protein export from the nucleus was examined in the budding yeast Saccharomyces cerevisiae. NPL3 encodes an RNA-binding protein that shuttles in and out of the nucleus. Export of poly(A)+ RNA has been shown previously to be blocked in np13-1 mutants. To understand the role of Np13p in RN...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.10.10.1233
更新日期：1996-05-15 00:00:00
abstract：:Proximal-distal outgrowth of the vertebrate limb bud is regulated by the apical ectodermal ridge (AER), which forms at an invariant position along the dorsal-ventral (D/V) axis of the embryo. We have studied the genetic and cellular events that regulate AER formation in the mouse. In contrast to implications from prev...
journal_title：Genes & development
pub_type： 杂志文章
doi：
更新日期：2000-06-01 00:00:00
abstract：:In the metazoan germline, piwi proteins and associated piwi-interacting RNAs (piRNAs) provide a defense system against the expression of transposable elements. In the cytoplasm, piRNA sequences guide piwi complexes to destroy complementary transposon transcripts by endonucleolytic cleavage. However, some piwi family m...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.209841.112
更新日期：2013-02-15 00:00:00
abstract：:H4K20 methylation is a broad chromatin modification that has been linked with diverse epigenetic functions. Several enzymes target H4K20 methylation, consistent with distinct mono-, di-, and trimethylation states controlling different biological outputs. To analyze the roles of H4K20 methylation states, we generated c...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.476008
更新日期：2008-08-01 00:00:00
abstract：:Cell for cell, probably no human cancer is as aggressive as melanoma. It is among a handful of cancers whose dimensions are reported in millimeters. Tumor thickness approaching 4 mm presents a high risk of metastasis, and a diagnosis of metastatic melanoma carries with it an abysmal median survival of 6-9 mo. What fea...
journal_title：Genes & development
pub_type： 杂志文章,评审
doi：10.1101/gad.1437206
更新日期：2006-08-15 00:00:00
abstract：:Transgenic mice expressing wild-type murine p53 under the control of the mouse mammary tumor virus long terminal repeat (MMTV LTR) undergo progressive renal failure due to abnormal kidney development. Similar phenotypes are observed in two transgenic lines that express wild-type p53 within the ureteric bud but not in ...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.10.7.836
更新日期：1996-04-01 00:00:00
abstract：:Signaling by the hormones brassinosteroid (BR) and gibberellin (GA) is critical to normal plant growth and development and is required for hypocotyl elongation in response to dark and elevated temperatures. Active BR signaling is essential for GA promotion of hypocotyl growth and suppresses the dwarf phenotype of GA m...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.243675.114
更新日期：2014-08-01 00:00:00
abstract：:Long noncoding RNAs (lncRNAs) can trigger repressive chromatin, but how they recruit silencing factors remains unclear. In Schizosaccharomyces pombe, heterochromatin assembly on transcribed noncoding pericentromeric repeats requires both RNAi and RNAi-independent mechanisms. In Saccharomyces cerevisiae, which lacks a ...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.226019.113
更新日期：2013-09-01 00:00:00
abstract：:The acetylation state of histones plays a central role in determining gene expression in chromatin. The reestablishment of the acetylation state of nucleosomes after DNA replication and chromatin assembly requires both deacetylation and acetylation of specific lysine residues on newly incorporated histones. In this st...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.929001
更新日期：2001-12-01 00:00:00
abstract：:Epithelial-mesenchymal transition (EMT) is thought to be an important, possibly essential, component of the process of tumor dissemination and metastasis. About 20%-30% of Hras mutant mouse skin carcinomas induced by chemical initiation/promotion protocols have undergone EMT. Reduced exposure to TPA-induced chronic in...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.210427.112
更新日期：2013-03-15 00:00:00
abstract：:Activation and repression of transcription in eukaryotes involve changes in the chromatin fiber that can be accomplished by covalent modification of the histone tails or the replacement of the canonical histones with other variants. Here we show that the histone H2A variant of Drosophila melanogaster, H2Av, localizes ...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1259105
更新日期：2005-01-01 00:00:00
abstract：:Macrophage activation by the proinflammatory cytokine interferon-gamma (IFN-gamma) is a critical component of the host innate response to bacterial pathogenesis. However, the precise nature of the IFN-gamma-induced activation pathway is not known. Here we show using genome-wide expression and chromatin-binding profili...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1553007
更新日期：2007-08-01 00:00:00
abstract：:We describe the developmental effects of the expression of a normal and mutated Drosophila ras gene ras2 in transgenic Drosophila. A Gly14----Val14 mutant (Gly14 in ras2 is equivalent to Gly12 in mammalian ras proteins) was constructed in ras2 by site-directed mutagenesis. Inducible constructs of ras2 and ras2Val14 we...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.2.5.567
更新日期：1988-05-01 00:00:00
abstract：:The product of the zygotically active decapentaplegic (dpp) gene appears to function as a morphogen that specifies positional information in the dorsal half of the Drosophila embryo. The dorsal-specific transcription of dpp is the key step in establishing a morphogen gradient. We demonstrate here that multiple regions...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.7.4.694
更新日期：1993-04-01 00:00:00
abstract：:Ribosomal protein synthesis ceases upon maturation of Xenopus oocytes. We find that this cessation results from the dissociation of ribosomal protein mRNAs from polysomes and is accompanied by the deadenylation of these transcripts. A synthetic mRNA encoding ribosomal protein L1, microinjected into stage VI oocytes, i...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.2.5.598
更新日期：1988-05-01 00:00:00
abstract：:Although MAX is regarded as an obligate dimerization partner for MYC, its function in normal development and neoplasia is poorly defined. We show that B-cell-specific deletion of Max has a modest effect on B-cell development but completely abrogates Eµ-Myc-driven lymphomagenesis. While Max loss affects only a few hund...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.325878.119
更新日期：2019-09-01 00:00:00
abstract：:During embryonic development in vertebrates, the endoderm becomes patterned along the anteroposterior axis to produce distinct derivatives. How this regulation is controlled is not well understood. We report that the zebrafish hairy/enhancer of split [E(spl)]-related gene her5 plays a critical role in this process. At...
journal_title：Genes & development
pub_type： 杂志文章
doi：
更新日期：2000-07-01 00:00:00
abstract：:The Notch signaling pathway is essential for embryonic vascular development in vertebrates. Here we show that mouse embryos heterozygous for a targeted mutation in the gene encoding the DLL4 ligand exhibit haploinsufficient lethality because of defects in vascular remodeling. We also describe vascular defects in embry...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1239204
更新日期：2004-10-15 00:00:00
