Abstract:
:In the metazoan germline, piwi proteins and associated piwi-interacting RNAs (piRNAs) provide a defense system against the expression of transposable elements. In the cytoplasm, piRNA sequences guide piwi complexes to destroy complementary transposon transcripts by endonucleolytic cleavage. However, some piwi family members are nuclear, raising the possibility of alternative pathways for piRNA-mediated regulation of gene expression. We found that Drosophila Piwi is recruited to chromatin, colocalizing with RNA polymerase II (Pol II) on polytene chromosomes. Knockdown of Piwi in the germline increases expression of transposable elements that are targeted by piRNAs, whereas protein-coding genes remain largely unaffected. Derepression of transposons upon Piwi depletion correlates with increased occupancy of Pol II on their promoters. Expression of piRNAs that target a reporter construct results in a decrease in Pol II occupancy and an increase in repressive H3K9me3 marks and heterochromatin protein 1 (HP1) on the reporter locus. Our results indicate that Piwi identifies targets complementary to the associated piRNA and induces transcriptional repression by establishing a repressive chromatin state when correct targets are found.
journal_name
Genes Devjournal_title
Genes & developmentauthors
Le Thomas A,Rogers AK,Webster A,Marinov GK,Liao SE,Perkins EM,Hur JK,Aravin AA,Tóth KFdoi
10.1101/gad.209841.112subject
Has Abstractpub_date
2013-02-15 00:00:00pages
390-9issue
4eissn
0890-9369issn
1549-5477pii
gad.209841.112journal_volume
27pub_type
杂志文章abstract::Alu repetitive sequences are the most abundant short interspersed DNA elements in the human genome. Full-length Alu elements are composed of two tandem sequence monomers, the left and right Alu arms, both derived from the 7SL signal recognition particle RNA. Since Alu elements are common in protein-coding genes, they ...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.197467.112
更新日期:2012-09-01 00:00:00
abstract::A transgenic mouse model for retinoblastoma was produced previously by directing SV40 T antigen expression to retinal photoreceptor cells using the promoter of the interstitial retinol-binding protein (IRBP) gene. This gene becomes active prior to the terminal differentiation of photoreceptors. Because T antigen-trans...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.8.11.1300
更新日期:1994-06-01 00:00:00
abstract::The liver regenerates upon partial hepatectomy (PH) as terminally differentiated hepatocytes undergo a tremendous proliferative process. CREM gene expression is powerfully induced during liver regeneration. We show that cell proliferation is significantly reduced upon PH in CREM-/- mice. There is a reduction in DNA sy...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.12.23.3639
更新日期:1998-12-01 00:00:00
abstract::The histone regulatory (HIR) and histone promoter control (HPC) repressor proteins regulate three of the four histone gene loci during the Saccharomyces cerevisiae cell cycle. Here, we demonstrate that Hir1, Hir2, Hir3, and Hpc2 proteins form a stable HIR repressor complex. The HIR complex promotes histone deposition ...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.1341105
更新日期:2005-11-01 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.285684.116
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abstract::Gene dosage alterations caused by aneuploidy are a common feature of most cancers yet pose severe proteotoxic challenges. Therefore, cells have evolved various dosage compensation mechanisms to limit the damage caused by the ensuing protein level imbalances. For instance, for heteromeric protein complexes, excess nons...
journal_title:Genes & development
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abstract::Smads regulate transcription of defined genes in response to TGF-beta receptor activation, although the mechanisms of Smad-mediated transcription are not well understood. We demonstrate that the TGF-beta-inducible Smad3 uses the tumor suppressor Smad4/DPC4 and CBP/p300 as transcriptional coactivators, which associate ...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.12.14.2153
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journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.204776.112
更新日期:2013-01-15 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.13.8.1037
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journal_title:Genes & development
pub_type: 杂志文章
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journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.2.2.150
更新日期:1988-02-01 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.10.8.921
更新日期:1996-04-15 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.2.5.517
更新日期:1988-05-01 00:00:00
abstract::The allosteric and torpedo models have been used for 30 yr to explain how transcription terminates on protein-coding genes. The former invokes termination via conformational changes in the transcription complex and the latter proposes that degradation of the downstream product of poly(A) signal (PAS) processing is imp...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.332833.119
更新日期:2020-01-01 00:00:00
abstract::Identifying modifiers of dosage-sensitive genes involved in neurodegenerative disorders is imperative to discover novel genetic risk factors and potential therapeutic entry points. In this study, we focus on Ataxin-1 (ATXN1), a dosage-sensitive gene involved in the neurodegenerative disease spinocerebellar ataxia type...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.339317.120
更新日期:2020-09-01 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
doi:
更新日期:2000-01-01 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.246538.114
更新日期:2014-11-15 00:00:00
abstract::Stomata are specialized cellular structures in the epidermis of aerial plant organs that control gas exchange (H(2)O release and CO(2) uptake) between leaves and the atmosphere by modulating the aperture of a pore flanked by two guard cells. Stomata are nonrandomly distributed, and their density is controlled by endog...
journal_title:Genes & development
pub_type: 杂志文章
doi:
更新日期:2000-05-01 00:00:00
abstract::Many tumors continuously depend on the initiating oncogenes, but whether this extends to their downstream targets is unclear. In this issue of Genes & Development, Sodir and colleagues (pp. 907-916) demonstrate an essential role for endogenous Myc proteins in maintaining the tumor microenvironment, providing an unexpe...
journal_title:Genes & development
pub_type: 评论,杂志文章
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更新日期:2011-05-01 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
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journal_title:Genes & development
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journal_title:Genes & development
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更新日期:2004-08-01 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.1267905
更新日期:2005-02-15 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.1832209
更新日期:2009-12-01 00:00:00
abstract::The embryonic ectoderm in Drosophila displays a highly organized arrangement of specific structures along the dorsal-ventral axis. To establish this characteristic design, cells must receive instructive cues regarding their position. We present evidence that during stages 8-9 of embryonic development, the Drosophila E...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.7.10.1937
更新日期:1993-10-01 00:00:00
abstract::The Neurospora crassa clock-controlled gene (ccg-2) is transcriptionally activated by the circadian clock in a time-of-day-specific manner. Transcript and sequence analyses of ccg-2 reveal that the predicted Ccg-2 polypeptide bears significant similarity to a class of low-molecular-weight, cysteine-rich, hydrophobic p...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.6.12a.2382
更新日期:1992-12-01 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
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更新日期:2003-02-01 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
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更新日期:2003-03-15 00:00:00
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journal_title:Genes & development
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更新日期:2009-06-15 00:00:00
abstract::Erasure and subsequent reinstatement of DNA methylation in the germline, especially at imprinted CpG islands (CGIs), is crucial to embryogenesis in mammals. The mechanisms underlying DNA methylation establishment remain poorly understood, but a number of post-translational modifications of histones are implicated in a...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.271353.115
更新日期:2015-12-01 00:00:00