Abstract：
:The connection between RNA and protein export from the nucleus was examined in the budding yeast Saccharomyces cerevisiae. NPL3 encodes an RNA-binding protein that shuttles in and out of the nucleus. Export of poly(A)+ RNA has been shown previously to be blocked in np13-1 mutants. To understand the role of Np13p in RNA export, we have developed a novel assay that effectively uncouples nuclear protein export from reimport. With this assay, we show that Np13p satisfies several of the predicted requirements for a protein carrier for mRNA export. Temperature-sensitive mutations in the RNA recognition motifs of Np13p result in nuclear accumulation of poly(A)+ RNA. One such mutation prevents nuclear export of Np13p. Moreover, Np13p export depends on ongoing RNA polymerase II transcription. Export ceases in either the presence of the RNA synthesis inhibitor thiolutin or in a temperature-sensitive RNA polymerase (rpb1) mutant. Together, these findings support a model in which Np13p exits the nucleus in association with poly(A)+ RNA, deposits the RNA in the cytoplasm, and is rapidly reimported for another cycle of export.
journal_name
Genes Devjournal_title
Genes & developmentauthors
Lee MS,Henry M,Silver PAdoi
10.1101/gad.10.10.1233subject
Has Abstractpub_date
1996-05-15 00:00:00pages
1233-46issue
10eissn
0890-9369issn
1549-5477journal_volume
10pub_type
杂志文章abstract：:The mouse Nedd5 gene encodes a 41.5-kD GTPase similar to the Saccharomyces and Drosophila septins essential for cytokinesis. Nedd5 accumulates near the contractile ring from anaphase through telophase, and finally condenses into the midbody. Microinjection of anti-Nedd5 antibody interferes with cytokinesis, giving ris...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.11.12.1535
更新日期：1997-06-15 00:00:00
abstract：:We showed previously that the MAP kinase ERK2 is essential for aggregation. erk2 null cells lack cAMP stimulation of adenylyl cyclase and thus cannot relay the cAMP chemotactic signal, although the cells chemotax to cAMP (Segall et al. 1995). In this paper we have examined the role of ERK2 in controlling developmental...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.10.1.118
更新日期：1996-01-01 00:00:00
abstract：:Transcription factor p53 forms a network with associated factors to regulate the cell cycle and apoptosis in response to environmental stresses. However, there is currently no direct genetic evidence to show if or how the p53 pathway functions during organogenesis. Here we present evidence to show that the zebrafish d...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1366405
更新日期：2005-12-01 00:00:00
abstract：:Alu repetitive sequences are the most abundant short interspersed DNA elements in the human genome. Full-length Alu elements are composed of two tandem sequence monomers, the left and right Alu arms, both derived from the 7SL signal recognition particle RNA. Since Alu elements are common in protein-coding genes, they ...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.197467.112
更新日期：2012-09-01 00:00:00
abstract：:Lipids play crucial roles in many aspects of glial cell biology, affecting processes ranging from myelin membrane biosynthesis to axo-glial interactions. In order to study the role of lipid metabolism in myelinating glial cells, we specifically deleted in Schwann cells the Lpin1 gene, which encodes the Mg2+-dependent ...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1638008
更新日期：2008-06-15 00:00:00
abstract：:The Caenorhabditis elegans caudal homolog, pal-1, is required for neurogenesis in the male tail. We show that expression of pal-1 in the postembryonic neuroblast cell V6 can be initiated by two alternate pathways. One pathway, acting in wild type, requires a regulatory element in the fifth pal-1 intron. The other path...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.814700
更新日期：2000-09-01 00:00:00
abstract：:The gene encoding yeast U6 snRNA that is transcribed by RNA polymerase III (Pol III) contains both a TATA box upstream of the transcription start site and a downstream binding site for the factor TFIIIC. This juxtaposition of elements typical of both Pol II- and Pol III-transcribed genes raises the question of how pol...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.9.7.832
更新日期：1995-04-01 00:00:00
abstract：:Ubiquitin-mediated proteolysis controls the abundance of many cell cycle regulatory proteins. Recent work in Saccharomyces cerevisiae suggests that a complex consisting of Cdc53, Skp1, and a third component known as an F-box protein (termed SCF) in combination with Cdc34 specifically targets regulatory proteins for de...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.12.16.2587
更新日期：1998-08-15 00:00:00
abstract：:Start sites of DNA replication are marked by the origin recognition complex (ORC), which coordinates Mcm2-7 helicase loading to form the prereplicative complex (pre-RC). Although pre-RC assembly is well characterized in vitro, the process is poorly understood within the local chromatin environment surrounding replicat...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.247924.114
更新日期：2015-01-15 00:00:00
abstract：:Appreciable advances into the process of transcript elongation by RNA polymerase II (RNAP II) have identified this stage as a dynamic and highly regulated step of the transcription cycle. Here, we discuss the many factors that regulate the elongation stage of transcription. Our discussion includes the classical elonga...
journal_title：Genes & development
pub_type： 杂志文章,评审
doi：10.1101/gad.1235904
更新日期：2004-10-15 00:00:00
abstract：:The PWI motif is a highly conserved domain of unknown function in the SRm160 splicing and 3'-end cleavage-stimulatory factor, as well as in several other known or putative pre-mRNA processing components. We show here that the PWI motif is a new type of RNA/DNA-binding domain that has an equal preference for single- an...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1060403
更新日期：2003-02-15 00:00:00
abstract：:E2F is a cellular, sequence-specific DNA-binding factor that binds to pairs of sites that occur upstream of the E1A and E2 early mRNA cap sites. During adenovirus infection, there is induction of a form of E2F that binds cooperatively to the pair of sites in the E2 control region. Production of the infection-specific ...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.3.7.1062
更新日期：1989-07-01 00:00:00
abstract：:Cells have evolved checkpoint responses to arrest or delay the cell cycle, activate DNA repair networks, or induce apoptosis after genomic perturbation. Cells have also evolved the translesion synthesis processes to tolerate genomic lesions by either error-free or error-prone repair. Here, we show that after a replica...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1043203
更新日期：2003-01-01 00:00:00
abstract：:In petunia flowers, the loci an1, an2, and an11 control the pigmentation of the flower by stimulating the transcription of anthocyanin biosynthetic genes. The an1 and an2 locus were recently cloned and encode a basic helix-loop-helix (bHLH) and MYB-domain transcriptional activator, respectively. Here, we report the is...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.11.11.1422
更新日期：1997-06-01 00:00:00
abstract：:Amplification of 8p11-12 in human breast cancers is associated with increased proliferation and tumor grade and reduced metastasis-free patient survival. We identified Zeppo1 (zinc finger elbow-related proline domain protein 1) (FLJ14299/ZNF703) within this amplicon as a regulator of cell adhesion, migration, and prol...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1998111
更新日期：2011-03-01 00:00:00
abstract：:A new gene, SCH9, was isolated from Saccharomyces cerevisiae by its ability to complement a cdc25ts mutation. Sequence analysis indicates that it encodes a 90,000-dalton protein with a carboxy-terminal domain homologous to yeast and mammalian cAMP-dependent protein kinase catalytic subunits. In addition to suppressing...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.2.5.517
更新日期：1988-05-01 00:00:00
abstract：:Sex determination is regulated by diverse pathways. Although upstream signals vary, a cysteine-rich DNA-binding domain (the DM motif) is conserved within downstream transcription factors of Drosophila melanogaster (Doublesex) and Caenorhabditis elegans (MAB-3). Vertebrate DM genes have likewise been identified and, re...
journal_title：Genes & development
pub_type： 杂志文章
doi：
更新日期：2000-07-15 00:00:00
abstract：:By studying neuroectoderm formation in the absence of mesoderm and mesectoderm in mutants of the zygotic genes snail and twist, we have found that the number of neuroblasts is not reduced in these mutants, suggesting that mesoderm and mesectoderm are not essential for the initiation of neural development. The position...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.5.9.1577
更新日期：1991-09-01 00:00:00
abstract：:Neurofibromatosis type 1 (NF1) is a prevalent genetic disorder that affects growth properties of neural-crest-derived cell populations. In addition, approximately one-half of NF1 patients exhibit learning disabilities. To characterize NF1 function both in vitro and in vivo, we circumvent the embryonic lethality of NF1...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.862101
更新日期：2001-04-01 00:00:00
abstract：:Establishment of the steroid-producing Leydig cell lineage is an event downstream of Sry that is critical for masculinization of mammalian embryos. Neither the origin of fetal Leydig cell precursors nor the signaling pathway that specifies the Leydig cell lineage is known. Based on the sex-specific expression patterns...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.981202
更新日期：2002-06-01 00:00:00
abstract：:Orexin A and Orexin B (also known as hypocretins) are neuropeptides that bind two related G-coupled protein receptors (OXR1 and OXR2) and thus induce wakefulness, food consumption, and locomotion. Conversely, deletion of the orexin gene in mice produces a condition similar to canine and human narcolepsy. Despite the c...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1584307
更新日期：2007-11-15 00:00:00
abstract：:Most eukaryotic mRNAs are translated using a cap-dependent mechanism of translation. However, approximately 10% of mammalian mRNAs initiate translation using a cap-independent mechanism that is not well understood. These mRNAs contain an internal ribosome entry site (IRES) located in the 5' untranslated region. The cr...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1832209
更新日期：2009-12-01 00:00:00
abstract：:Our laboratory has been utilizing the Escherichia coli outer membrane protein LamB to study the mechanism of protein localization. Various lines of evidence suggest that, in addition to a signal sequence, regions within the mature protein are required for efficient localization. In particular, studies using LamB-LacZ ...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1.2.185
更新日期：1987-04-01 00:00:00
abstract：:It is now well appreciated that the apoptosome, which governs caspase-dependent cell death, also drives nonapoptotic caspase activation to remodel cells. However, the determinants that specify whether the apoptosome acts to kill or remodel have yet to be identified. Here we report that Tango7 collaborates with the Dro...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.219287.113
更新日期：2013-08-01 00:00:00
abstract：:Overexpression of the type II transmembrane serine protease matriptase is a highly consistent feature of human epithelial tumors. Here we show that matriptase possesses a strong oncogenic potential when unopposed by its endogenous inhibitor, HAI-1. Modest orthotopic overexpression of matriptase in the skin of transgen...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1300705
更新日期：2005-08-15 00:00:00
abstract：:Transformation of primary rodent cells by the adenovirus E1A and E1B oncogenes is a two-step process, where E1A-dependent induction of proliferation is coupled to E1B-dependent suppression of programmed cell death (apoptosis). The E1B gene encodes two distinct transforming proteins, the 19K and 55K proteins, both of w...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.7.4.546
更新日期：1993-04-01 00:00:00
abstract：:Genetic screening and selection procedures employing a secA-lacZ fusion strain repeatedly have yielded mutations in four genes affecting the protein export pathway of Escherichia coli. These genes are secA, secD, prlA/secY, and secE. We discuss the significance of the failure to find new sec genes after extensive use ...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.3.7.1035
更新日期：1989-07-01 00:00:00
abstract：:Transcription factor IIA has been shown to interact with the TATA-binding protein and to act early during preinitiation complex formation. The human factor is composed of three subunits (alpha, beta, gamma). A human cDNA clone encoding the largest subunit of TFIIA (alpha) was isolated. The recombinant alpha polypeptid...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.7.11.2246
更新日期：1993-11-01 00:00:00
abstract：:Neurofibromatosis type 1 (NF1) is a common neurodevelopmental disorder caused by impaired function of the neurofibromin RAS regulator. Using a combination of Nf1 genetically engineered mice and pharmacological/genetic inhibition approaches, we report that neurofibromin differentially controls neural stem cell (NSC) pr...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.261677.115
更新日期：2015-08-15 00:00:00
abstract：:To define the functional pathways regulating epithelial cell migration, we performed a genome-wide RNAi screen using 55,000 pooled lentiviral shRNAs targeting ∼11,000 genes, selecting for transduced cells with increased motility. A stringent validation protocol generated a set of 31 genes representing diverse pathways...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1989110
更新日期：2010-12-01 00:00:00
