Abstract:
:E2F is a cellular, sequence-specific DNA-binding factor that binds to pairs of sites that occur upstream of the E1A and E2 early mRNA cap sites. During adenovirus infection, there is induction of a form of E2F that binds cooperatively to the pair of sites in the E2 control region. Production of the infection-specific E2F activity is dependent on early region 4 (E4), as extracts of cells infected with a mutant that lacks E4 did not contain this activity. Instead, two new forms of E2F were seen with the E4 mutant. Infection with mutant viruses unable to make E1A gene products produced the wild-type infection-specific E2F activity after a delay. Mutations in the E1B-55 kD-, E1B-21 kD-, E2-72 kD-, and E3-coding regions had no effect on production of infection-specific E2F. Analysis of cell lines confirmed the results obtained with mutant viruses. Cells that expressed E1A but not E4 genes (e.g., 293 cells) did not contain infection-specific E2F. Cell lines that expressed the E4 gene contained the activity. These observations demonstrate that E4 participates in the infection-induced change in E2F-binding activity. The data are consistent with E1A playing an indirect role in the process by mediating the efficient expression of E4 gene products which, in turn, induce the alteration in E2F activity.
journal_name
Genes Devjournal_title
Genes & developmentauthors
Hardy S,Engel DA,Shenk Tdoi
10.1101/gad.3.7.1062subject
Has Abstractpub_date
1989-07-01 00:00:00pages
1062-74issue
7eissn
0890-9369issn
1549-5477journal_volume
3pub_type
杂志文章abstract::Faithful inheritance of genetic information requires that DNA be copied only once each cell cycle. Initiation of DNA replication involves the establishment of a prereplication complex (pre-RC) and subsequent activation by CDK/cyclins, converting the pre-RC to a post-RC. The origin recognition complex (ORC), Cdc6p, and...
journal_title:Genes & development
pub_type: 杂志文章
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journal_title:Genes & development
pub_type: 杂志文章
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journal_title:Genes & development
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journal_title:Genes & development
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更新日期:2001-11-01 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
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journal_title:Genes & development
pub_type: 杂志文章
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journal_title:Genes & development
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journal_title:Genes & development
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更新日期:2003-07-15 00:00:00
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journal_title:Genes & development
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journal_title:Genes & development
pub_type: 杂志文章
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更新日期:2006-01-01 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.8.17.2086
更新日期:1994-09-01 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
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更新日期:2014-06-01 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
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更新日期:2007-12-15 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
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更新日期:2013-08-01 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
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更新日期:2008-09-15 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.9.21.2623
更新日期:1995-11-01 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
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更新日期:2008-05-01 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
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journal_title:Genes & development
pub_type: 杂志文章
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更新日期:2001-11-15 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
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更新日期:2004-01-15 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
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更新日期:1998-04-01 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
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更新日期:1995-07-15 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
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更新日期:2011-08-01 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
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更新日期:2008-08-15 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
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journal_title:Genes & development
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journal_title:Genes & development
pub_type: 杂志文章
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更新日期:2010-05-01 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
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更新日期:2002-10-15 00:00:00