Abstract:
:Spliceosome assembly has been characterized as the ordered association of the snRNP particles U1, U2, and U4/U6.U5 onto pre-mRNA. We have used an in vitro trans-splicing/cross-linking system in Saccharomyces cerevisiae nuclear extracts to examine the first step of this process, 5' splice site recognition. This trans-splicing reaction has ATP, Mg(2+), and splice-site sequence requirements similar to those of cis-splicing reactions. Using this system, we identified and characterized a novel U4-5' splice site interaction that is ATP-dependent, but does not require the branch point, the 3' splice site, or the 5' end of the U1 snRNA. Additionally, we identified several ATP-dependent U6 cross-links at the 5' splice site, indicating that different regions of U6 sample it before a U6-5' splice site interaction is stabilized that persists through the first step of splicing. This work provides evidence for ATP-dependent U4/U6 association with the 5' splice site independent of ATP-mediated U2 association with the branch point. Furthermore, it defines specific nucleotides in U4 and U6 that interact with the 5' splice site at this early stage, even in the absence of base-pairing with the U1 snRNA.
journal_name
Genes Devjournal_title
Genes & developmentauthors
Johnson TL,Abelson Jdoi
10.1101/gad.895601subject
Has Abstractpub_date
2001-08-01 00:00:00pages
1957-70issue
15eissn
0890-9369issn
1549-5477journal_volume
15pub_type
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更新日期:2017-06-01 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
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更新日期:2010-07-01 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.13.8.1037
更新日期:1999-04-15 00:00:00
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journal_title:Genes & development
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