DNA damage activates p53 through a phosphorylation-acetylation cascade.

abstract：:Metazoans start embryogenesis with a relatively naïve genome. The transcriptionally inert, late-replicating heterochromatic regions, including the constitutive heterochromatin on repetitive sequences near centromeres and telomeres, need to be re-established during development. To explore the events initiating heteroch...

journal_title：Genes & development

authors： Yuan K,O'Farrell PH

更新日期：2016-03-01 00:00:00

abstract：:Activation of the CLB gene cluster through the assembly of Mcm1p-Fkh2p complexes at target promoters is essential for mitotic entry and transition through M phase. We show that the activation of this mitotic transcriptional program is dependent on the recruitment of Ndd1p, a coactivator that performs its essential fun...

journal_title：Genes & development

authors： Reynolds D,Shi BJ,McLean C,Katsis F,Kemp B,Dalton S

更新日期：2003-07-15 00:00:00

abstract：:Cyclin D1 is part of a cell cycle control node consistently deregulated in most human cancers. However, studies with cyclin D1-null mice indicate that it is dispensable for normal mouse development as well as cell growth in culture. Here, we provide evidence that ras-mediated tumorigenesis depends on signaling pathway...

journal_title：Genes & development

authors： Robles AI,Rodriguez-Puebla ML,Glick AB,Trempus C,Hansen L,Sicinski P,Tennant RW,Weinberg RA,Yuspa SH,Conti CJ

更新日期：1998-08-15 00:00:00

abstract：:Stem cells have been identified as a source of virtually all highly differentiated cells that are replenished during the lifetime of an animal. The critical balance between stem and differentiated cell populations is crucial for the long-term maintenance of functional tissue types. Stem cells maintain this balance by ...

journal_title：Genes & development

authors： Martinez-Agosto JA,Mikkola HK,Hartenstein V,Banerjee U

更新日期：2007-12-01 00:00:00

abstract：:Chemical mutagenesis in the mouse is a powerful approach for phenotype-driven genetics, but questions remain about the efficiency with which new mutations ascertained by their phenotype can be localized and identified, and that knowledge applied to a specific biological problem. During a global screen for dominant phe...

journal_title：Genes & development

authors： Fitch KR,McGowan KA,van Raamsdonk CD,Fuchs H,Lee D,Puech A,Hérault Y,Threadgill DW,Hrabé de Angelis M,Barsh GS

更新日期：2003-01-15 00:00:00

abstract：:To define the functional pathways regulating epithelial cell migration, we performed a genome-wide RNAi screen using 55,000 pooled lentiviral shRNAs targeting ∼11,000 genes, selecting for transduced cells with increased motility. A stringent validation protocol generated a set of 31 genes representing diverse pathways...

journal_title：Genes & development

authors： Smolen GA,Zhang J,Zubrowski MJ,Edelman EJ,Luo B,Yu M,Ng LW,Scherber CM,Schott BJ,Ramaswamy S,Irimia D,Root DE,Haber DA

更新日期：2010-12-01 00:00:00

abstract：:The Rho family of small GTPases are critical elements involved in the regulation of signal transduction cascades from extracellular stimuli to the cell nucleus, including the JNK/SAPK signaling pathway, the c-fos serum response factor, and the p70 S6 kinase. Here we report a novel signaling pathway activated by the Rh...

journal_title：Genes & development

authors： Perona R,Montaner S,Saniger L,Sánchez-Pérez I,Bravo R,Lacal JC

更新日期：1997-02-15 00:00:00

abstract：:The C-terminal domain of the Escherichia coli RNA polymerase (RNAP) alpha subunit (alphaCTD) stimulates transcription initiation by interacting with upstream (UP) element DNA and a variety of transcription activators. Here we identify specific substitutions in region 4.2 of sigma 70 (sigma(70)) and in alphaCTD that de...

journal_title：Genes & development

authors： Ross W,Schneider DA,Paul BJ,Mertens A,Gourse RL

更新日期：2003-05-15 00:00:00

abstract：:Kinetochores play an essential role in chromosome segregation by forming dynamic connections with spindle microtubules. Here, we identify a set of 10 copurifying kinetochore proteins from Caenorhabditis elegans, seven of which were previously uncharacterized. Using in vivo assays to monitor chromosome segregation, kin...

journal_title：Genes & development

authors： Cheeseman IM,Niessen S,Anderson S,Hyndman F,Yates JR 3rd,Oegema K,Desai A

更新日期：2004-09-15 00:00:00

abstract：:Protein kinases are key regulators of cellular processes. In spite of considerable effort, a full understanding of the pathways they participate in remains elusive. We globally investigated the proteins that interact with the majority of yeast protein kinases using protein microarrays. Eighty-five kinases were purifie...

journal_title：Genes & development

authors： Fasolo J,Sboner A,Sun MG,Yu H,Chen R,Sharon D,Kim PM,Gerstein M,Snyder M

更新日期：2011-04-01 00:00:00

abstract：:Growth of numerous cancer types is believed to be driven by a subpopulation of poorly differentiated cells, often referred to as cancer stem cells (CSCs), that have the capacity for self-renewal, tumor initiation, and generation of nontumorigenic progeny. Despite their potentially key role in tumor establishment and m...

journal_title：Genes & development

authors： Janiszewska M,Suvà ML,Riggi N,Houtkooper RH,Auwerx J,Clément-Schatlo V,Radovanovic I,Rheinbay E,Provero P,Stamenkovic I

更新日期：2012-09-01 00:00:00

abstract：:We isolated a novel bHLH protein gene Mesp2 (for mesoderm posterior 2) that cross-hybridizes with Mesp1 expressed in the early mouse mesoderm. Mesp2 is expressed in the rostral presomitic mesoderm, but down-regulated immediately after the formation of the segmented somites. To determine the function of MesP2 protein (...

journal_title：Genes & development

authors： Saga Y,Hata N,Koseki H,Taketo MM

更新日期：1997-07-15 00:00:00

abstract：:Intron homing in phage T4 occurs in the context of recombination-dependent replication, by virtue of intron-encoded endonucleolytic activity. After the td intron endonuclease I-TevI cleaves the intronless recipient 23 and 25 nucleotides upstream of the intron insertion site, exonucleolytic degradation is required for ...

journal_title：Genes & development

authors： Mueller JE,Smith D,Belfort M

更新日期：1996-09-01 00:00:00

abstract：:We generated and characterized greater than 500 Drosophila strains that carry single copies of a novel P-element enhancer detector. In the majority of the strains, the beta-galactosidase reporter gene in the P-transposon responds to nearby transcriptional regulatory sequences in the genome. A remarkable diversity of s...

journal_title：Genes & development

authors： Bellen HJ,O'Kane CJ,Wilson C,Grossniklaus U,Pearson RK,Gehring WJ

更新日期：1989-09-01 00:00:00

abstract：:The Arabidopsis APETALA3 (AP3) and PISTILLATA (PI) proteins are thought to act as transcription factors and are required for specifying floral organ identities. To define the nuclear localization signals within these proteins, we generated translational fusions of the coding regions of AP3 and PI to the bacterial uidA...

journal_title：Genes & development

authors： McGonigle B,Bouhidel K,Irish VF

更新日期：1996-07-15 00:00:00

abstract：:Oxidative tissues such as heart undergo a dramatic perinatal mitochondrial biogenesis to meet the high-energy demands after birth. PPARgamma coactivator-1 (PGC-1) alpha and beta have been implicated in the transcriptional control of cellular energy metabolism. Mice with combined deficiency of PGC-1alpha and PGC-1beta ...

journal_title：Genes & development

authors： Lai L,Leone TC,Zechner C,Schaeffer PJ,Kelly SM,Flanagan DP,Medeiros DM,Kovacs A,Kelly DP

更新日期：2008-07-15 00:00:00

abstract：:In budding yeast, a switch between the mutually exclusive pathways of cell cycle progression and conjugation is controlled at Start in late G1 phase. Mating pheromones promote conjugation by arresting cells in G1 phase before Start. Pheromone-induced cell cycle arrest requires a functional FAR1 gene. We have found tha...

journal_title：Genes & development

authors： McKinney JD,Chang F,Heintz N,Cross FR

更新日期：1993-05-01 00:00:00

abstract：:In response to skeletal muscle injury, satellite cells, which function as a myogenic stem cell population, become activated, expand through proliferation, and ultimately fuse with each other and with damaged myofibers to promote muscle regeneration. Here, we show that members of the Myocardin family of transcriptional...

journal_title：Genes & development

authors： Mokalled MH,Johnson AN,Creemers EE,Olson EN

更新日期：2012-01-15 00:00:00

abstract：:Pax6 is a key transcription factor in eye development, particularly in lens development, but its molecular action has not been clarified. We demonstrate that Pax6 initiates lens development by forming a molecular complex with SOX2 on the lens-specific enhancer elements, e.g., the delta-crystallin minimal enhancer DC5....

journal_title：Genes & development

authors： Kamachi Y,Uchikawa M,Tanouchi A,Sekido R,Kondoh H

更新日期：2001-05-15 00:00:00

abstract：:Genome-wide association studies (GWASs) have identified a genetic variant of moderate effect size at 6p21.1 associated with erythrocyte traits in humans. We show that this variant affects an erythroid-specific enhancer of CCND3. A Ccnd3 knockout mouse phenocopies these erythroid phenotypes, with a dramatic increase in...

journal_title：Genes & development

authors： Sankaran VG,Ludwig LS,Sicinska E,Xu J,Bauer DE,Eng JC,Patterson HC,Metcalf RA,Natkunam Y,Orkin SH,Sicinski P,Lander ES,Lodish HF

更新日期：2012-09-15 00:00:00

abstract：:Previous work has shown that rRNA synthesis is strongly inhibited in yeast top1-top2 double mutants. Here, we show that inactivation of yeast topoisomerases can have paradoxical effects on transcription by RNA polymerase I. For example, transcription of ribosomal minigenes on extrachromosomal plasmids is greatly stimu...

journal_title：Genes & development

authors： Schultz MC,Brill SJ,Ju Q,Sternglanz R,Reeder RH

更新日期：1992-07-01 00:00:00

abstract：:The lysosomal storage disorder galactosialidosis results from a primary deficiency of the protective protein/cathepsin A (PPCA), which in turn affects the activities of beta-galactosidase and neuraminidase. Mice homozygous for a null mutation at the PPCA locus present with signs of the disease shortly after birth and ...

journal_title：Genes & development

authors： Zhou XY,Morreau H,Rottier R,Davis D,Bonten E,Gillemans N,Wenger D,Grosveld FG,Doherty P,Suzuki K,Grosveld GC,d'Azzo A

更新日期：1995-11-01 00:00:00

abstract：:Transcription of the cell-cycle-regulated human histone genes increases approximately fivefold during S phase. One step toward the elucidation of the biochemical mechanisms that govern cell-cycle-regulated expression of these genes is to purify and characterize the transcription factors that regulate these promoters. ...

journal_title：Genes & development

authors： Dailey L,Roberts SB,Heintz N

更新日期：1988-12-01 00:00:00

abstract：:Innate immunity against pathogen infection is an evolutionarily conserved process among multicellular organisms. Arabidopsis SNC1 encodes a Resistance protein that combines attributes of multiple mammalian pattern recognition receptors. Utilizing snc1 as an autoimmune model, we identified a discrete protein complex co...

journal_title：Genes & development

authors： Palma K,Zhao Q,Cheng YT,Bi D,Monaghan J,Cheng W,Zhang Y,Li X

更新日期：2007-06-15 00:00:00

abstract：:Disruption of the balanced modulation of reversible tyrosine phosphorylation has been implicated in the etiology of various human cancers, including breast cancer. Protein Tyrosine Phosphatase N23 (PTPN23) resides in chromosomal region 3p21.3, which is hemizygously or homozygously lost in some breast cancer patients. ...

journal_title：Genes & development

authors： Zhang S,Fan G,Hao Y,Hammell M,Wilkinson JE,Tonks NK

更新日期：2017-10-01 00:00:00

abstract：:The neurofibromatosis type II (NF2) tumor suppressor encodes a putative cytoskeletal associated protein, the loss of which leads to the development of Schwann cell tumors associated with NF2 in humans. The NF2 protein merlin belongs to the band 4.1 family of proteins that link membrane proteins to the cytoskeleton and...

journal_title：Genes & development

authors： McClatchey AI,Saotome I,Ramesh V,Gusella JF,Jacks T

更新日期：1997-05-15 00:00:00

abstract：:The Drosophila somatic sex-determination regulatory pathway has been well studied, but little is known about the target genes that it ultimately controls. In a differential screen for sex-specific transcripts expressed in fly heads, we identified a highly male-enriched transcript encoding Takeout, a protein related to...

journal_title：Genes & development

authors： Dauwalder B,Tsujimoto S,Moss J,Mattox W

更新日期：2002-11-15 00:00:00

abstract：:The repression of the maternally inherited Igf2 allele has been proposed to depend on a methylation-sensitive chromatin insulator organized by the 11 zinc finger protein CTCF at the H19 imprinting control region (ICR). Here we document that point mutations of the nucleotides in physical contact with CTCF within the en...

journal_title：Genes & development

authors： Pant V,Mariano P,Kanduri C,Mattsson A,Lobanenkov V,Heuchel R,Ohlsson R

更新日期：2003-03-01 00:00:00

abstract：:Mammalian cells possess two amino acid-sensing kinases: general control nonderepressible 2 (GCN2) and mechanistic target of rapamycin complex 1 (mTORC1). Their combined effects orchestrate cellular adaptation to amino acid levels, but how their activities are coordinated remains poorly understood. Here, we demonstrate...

journal_title：Genes & development

authors： Ye J,Palm W,Peng M,King B,Lindsten T,Li MO,Koumenis C,Thompson CB

更新日期：2015-11-15 00:00:00

abstract：:Post-translational processes are essential for the generation and dynamics of mammalian circadian rhythms. In particular, phosphorylation of the key circadian protein PER2 precisely controls the period and phase of circadian oscillations. However, the mechanisms underlying that control are poorly understood. Here, we ...

journal_title：Genes & development

authors： Maier B,Wendt S,Vanselow JT,Wallach T,Reischl S,Oehmke S,Schlosser A,Kramer A

更新日期：2009-03-15 00:00:00