Expression of an activated ras gene causes developmental abnormalities in transgenic Drosophila melanogaster.

abstract：:We have developed a new technique, called 'toeprinting,' which has allowed a study of the tRNA-binding properties of Escherichia coli translation initiation complexes. In response to natural mRNAs, the initiator tRNA and a variety of elongator tRNAs bind to the same tRNA-binding site on the 30S ribosomal subunit as lo...

journal_title：Genes & development

authors： Hartz D,McPheeters DS,Gold L

更新日期：1989-12-01 00:00:00

abstract：:Mice carrying a germ-line null mutation of the prolactin receptor gene have been produced by gene targeting in embryonic stem cells. Heterozygous females showed almost complete failure of lactation attributable to greatly reduced mammary gland development after their first, but not subsequent, pregnancies. Homozygous ...

journal_title：Genes & development

authors： Ormandy CJ,Camus A,Barra J,Damotte D,Lucas B,Buteau H,Edery M,Brousse N,Babinet C,Binart N,Kelly PA

更新日期：1997-01-15 00:00:00

abstract：:The Caenorhabditis elegans caudal homolog, pal-1, is required for neurogenesis in the male tail. We show that expression of pal-1 in the postembryonic neuroblast cell V6 can be initiated by two alternate pathways. One pathway, acting in wild type, requires a regulatory element in the fifth pal-1 intron. The other path...

journal_title：Genes & development

authors： Zhang H,Emmons SW

更新日期：2000-09-01 00:00:00

abstract：:The HOP2 and MND1 genes are indispensable for meiotic recombination. The products of these genes associate to form a stable heterodimeric complex that binds DNA and stimulates the recombinase activity of Rad51 and Dmc1. Here we conduct molecular studies to delineate the action mechanism of the Hop2-Mnd1 complex. We pr...

journal_title：Genes & development

authors： Chi P,San Filippo J,Sehorn MG,Petukhova GV,Sung P

更新日期：2007-07-15 00:00:00

abstract：:Neurofibromatosis type 1 (NF1) is a common neurodevelopmental disorder caused by impaired function of the neurofibromin RAS regulator. Using a combination of Nf1 genetically engineered mice and pharmacological/genetic inhibition approaches, we report that neurofibromin differentially controls neural stem cell (NSC) pr...

journal_title：Genes & development

authors： Chen YH,Gianino SM,Gutmann DH

更新日期：2015-08-15 00:00:00

abstract：:Eukaryotic chromosomes reach their stable rod-shaped appearance in mitosis in a reaction dependent on the evolutionarily conserved condensin complex. Little is known about how and where condensin associates with chromosomes. Here, we analyze condensin binding to budding yeast chromosomes using high-resolution oligonuc...

journal_title：Genes & development

authors： D'Ambrosio C,Schmidt CK,Katou Y,Kelly G,Itoh T,Shirahige K,Uhlmann F

更新日期：2008-08-15 00:00:00

abstract：:We used Sarkosyl to analyze steps along the pathway of transcription initiation by RNA polymerase III. Sarkosyl (0.015%) inhibited transcription when present prior to incubation of RNA polymerase III, TFIIIB, and TFIIIC with the VAI gene, whereas it had no detectable effect on initiation or reinitiation of transcripti...

journal_title：Genes & development

authors： Kovelman R,Roeder RG

更新日期：1990-04-01 00:00:00

abstract：:The crucifer Arabidopsis thaliana has been used widely as a model organism for the study of plant development. We describe here the development of an efficient insertional mutagenesis system in Arabidopsis that permits identification of genes by their patterns of expression during development. Transposable elements of...

journal_title：Genes & development

authors： Sundaresan V,Springer P,Volpe T,Haward S,Jones JD,Dean C,Ma H,Martienssen R

更新日期：1995-07-15 00:00:00

abstract：:The LIM motif defines a double zinc finger structure found in proteins involved in cell fate determination and growth control. LIM proteins, which include LIM homeo domain, LIM kinase, focal adhesion, and LIM-only proteins, usually contain two or more LIM motifs clustered at their amino- or carboxy-terminal end. At pr...

journal_title：Genes & development

authors： Arber S,Caroni P

更新日期：1996-02-01 00:00:00

abstract：:Expression of both N-myc and c-myc is induced rapidly and dramatically in normal pre-B cells after stimulation with interleukin-7 (IL-7), a pre-B cell-specific growth factor. These IL-7-induced increases in N-myc and c-myc expression are mediated at the transcriptional level; for N-myc, a major portion of the inductio...

journal_title：Genes & development

authors： Morrow MA,Lee G,Gillis S,Yancopoulos GD,Alt FW

更新日期：1992-01-01 00:00:00

abstract：:RNA interference (RNAi) is a flexible gene silencing mechanism that responds to double-stranded RNA by suppressing homologous genes. Here, we report the characterization of RNAi effector complexes (RISCs) that contain small interfering RNAs and microRNAs (miRNAs). We identify two putative RNA-binding proteins, the Dro...

journal_title：Genes & development

authors： Caudy AA,Myers M,Hannon GJ,Hammond SM

更新日期：2002-10-01 00:00:00

abstract：:Expression of the gene encoding atrial natriuretic factor (ANF) during mouse cardiac embryogenesis was studied using in situ hybridization to histologic sections. ANF mRNA was first detected in a subpopulation of myocardial cells at day 8 of embryogenesis. During day 9, abundant hybridization of the ANF probe to the a...

journal_title：Genes & development

authors： Zeller R,Bloch KD,Williams BS,Arceci RJ,Seidman CE

更新日期：1987-09-01 00:00:00

abstract：:The gene for an essential protein subunit of nuclear RNase P from Saccharomyces cerevisiae has been cloned. The gene for this protein, RPP1, was identified by virtue of its homology with a human scleroderma autoimmune antigen, Rpp30, which copurifies with human RNase P. Epitope-tagged Rpp1 can be found in association ...

journal_title：Genes & development

authors： Stolc V,Altman S

更新日期：1997-11-01 00:00:00

abstract：:Plectin, a major linker and scaffolding protein of the cytoskeleton, has been shown to be essential for the mechanical integrity of skin, skeletal muscle, and heart. Studying fibroblast and astroglial cell cultures derived from plectin (-/-) mice, we found that their actin cytoskeleton, including focal adhesion contac...

journal_title：Genes & development

authors： Andrä K,Nikolic B,Stöcher M,Drenckhahn D,Wiche G

更新日期：1998-11-01 00:00:00

abstract：:Ribonucleotide reductase activity is essential for progression through the cell cycle, catalyzing the rate-limiting step for the production of deoxyribonucleotides needed for DNA synthesis. The enzymatic activity of the enzyme fluctuates in the cell cycle with an activity maximum in S phase. We have identified and cha...

journal_title：Genes & development

authors： Elledge SJ,Davis RW

更新日期：1990-05-01 00:00:00

abstract：:Transgenic mice expressing wild-type murine p53 under the control of the mouse mammary tumor virus long terminal repeat (MMTV LTR) undergo progressive renal failure due to abnormal kidney development. Similar phenotypes are observed in two transgenic lines that express wild-type p53 within the ureteric bud but not in ...

journal_title：Genes & development

authors： Godley LA,Kopp JB,Eckhaus M,Paglino JJ,Owens J,Varmus HE

更新日期：1996-04-01 00:00:00

abstract：:In Drosophila the function of the epidermal growth factor (EGF) receptor is modulated zygotically by three EGF-like proteins: Spitz (Spi), which is a potent activator; Vein (Vn), which is a moderate activator; and Argos (Aos), which is an inhibitor. Chimeric molecules were constructed in which the EGF domain of Vn was...

journal_title：Genes & development

authors： Schnepp B,Donaldson T,Grumbling G,Ostrowski S,Schweitzer R,Shilo BZ,Simcox A

更新日期：1998-04-01 00:00:00

abstract：:Asymmetric localization of cell fate determinants is a crucial step in neuroblast asymmetric divisions. Whereas several protein kinases have been shown to mediate this process, no protein phosphatase has so far been implicated. In a clonal screen of larval neuroblasts we identified the evolutionarily conserved Protein...

journal_title：Genes & development

authors： Sousa-Nunes R,Chia W,Somers WG

更新日期：2009-02-01 00:00:00

abstract：:The recombinational enhancer of the Hin inversion system in Salmonella stimulates recombination in vitro 150-fold in the presence of the Escherichia coli host factor Fis. To gain an understanding of the roles of the enhancer and Fis in stimulating the Hin-mediated inversion reaction, we have used nuclease and chemical...

journal_title：Genes & development

authors： Bruist MF,Glasgow AC,Johnson RC,Simon MI

更新日期：1987-10-01 00:00:00

abstract：:The eukaryotic ribosomal RNA (rRNA) is associated cotranscriptionally with numerous factors into an enormous 90S preribosomal particle that conducts early processing of small ribosomal subunits. The assembly pathway and structure of the 90S particle is poorly understood. Here, we affinity-purified and analyzed the con...

journal_title：Genes & development

authors： Zhang L,Wu C,Cai G,Chen S,Ye K

更新日期：2016-03-15 00:00:00

abstract：:Wilms Tumor, the most common pediatric kidney cancer, evolves from the failure of terminal differentiation of the embryonic kidney. Here we show that overexpression of the heterochronic regulator Lin28 during kidney development in mice markedly expands nephrogenic progenitors by blocking their final wave of differenti...

journal_title：Genes & development

authors： Urbach A,Yermalovich A,Zhang J,Spina CS,Zhu H,Perez-Atayde AR,Shukrun R,Charlton J,Sebire N,Mifsud W,Dekel B,Pritchard-Jones K,Daley GQ

更新日期：2014-05-01 00:00:00

abstract：:Faithful inheritance of genetic information requires that DNA be copied only once each cell cycle. Initiation of DNA replication involves the establishment of a prereplication complex (pre-RC) and subsequent activation by CDK/cyclins, converting the pre-RC to a post-RC. The origin recognition complex (ORC), Cdc6p, and...

journal_title：Genes & development

authors： Liang C,Stillman B

更新日期：1997-12-15 00:00:00

abstract：:Sex determination is regulated by diverse pathways. Although upstream signals vary, a cysteine-rich DNA-binding domain (the DM motif) is conserved within downstream transcription factors of Drosophila melanogaster (Doublesex) and Caenorhabditis elegans (MAB-3). Vertebrate DM genes have likewise been identified and, re...

journal_title：Genes & development

authors： Zhu L,Wilken J,Phillips NB,Narendra U,Chan G,Stratton SM,Kent SB,Weiss MA

更新日期：2000-07-15 00:00:00

abstract：:The PWI motif is a highly conserved domain of unknown function in the SRm160 splicing and 3'-end cleavage-stimulatory factor, as well as in several other known or putative pre-mRNA processing components. We show here that the PWI motif is a new type of RNA/DNA-binding domain that has an equal preference for single- an...

journal_title：Genes & development

authors： Szymczyna BR,Bowman J,McCracken S,Pineda-Lucena A,Lu Y,Cox B,Lambermon M,Graveley BR,Arrowsmith CH,Blencowe BJ

更新日期：2003-02-15 00:00:00

abstract：:Dosage compensation is a process required to balance the expression of X-linked genes between males and females. In Drosophila this is achieved by targeting the dosage compensation complex or the male-specific lethal (MSL) complex to the male X chromosome. In order to study the mechanism of targeting, we have studied ...

journal_title：Genes & development

authors： Kind J,Akhtar A

更新日期：2007-08-15 00:00:00

abstract：:We isolated a novel bHLH protein gene Mesp2 (for mesoderm posterior 2) that cross-hybridizes with Mesp1 expressed in the early mouse mesoderm. Mesp2 is expressed in the rostral presomitic mesoderm, but down-regulated immediately after the formation of the segmented somites. To determine the function of MesP2 protein (...

journal_title：Genes & development

authors： Saga Y,Hata N,Koseki H,Taketo MM

更新日期：1997-07-15 00:00:00

abstract：:Many short-lived mRNAs, including those encoding lymphokines, cytokines, and proto-oncogenes, contain an AU-rich sequence in their 3'-untranslated regions. These AU domains and, more specifically, AUUUA motifs within them, are widely thought to mediate the extreme instability of the corresponding mRNAs. This is most c...

journal_title：Genes & development

authors： Savant-Bhonsale S,Cleveland DW

更新日期：1992-10-01 00:00:00

abstract：:Regions on the surface of human TATA-box binding protein (TBP) required for activated transcription in vivo were defined by construction of a library of 89 surface residue mutants with radical substitutions that were assayed for their ability to support activated transcription in vivo, basal transcription in vitro, an...

journal_title：Genes & development

authors： Bryant GO,Martel LS,Burley SK,Berk AJ

更新日期：1996-10-01 00:00:00

abstract：:The sigma(28) protein is a member of the bacterial sigma(70)-family of transcription factors that directs RNA polymerase to flagellar late (class 3) promoters. The sigma(28) protein is regulated in response to flagellar assembly by the anti-sigma(28) factor FlgM. FlgM inhibits sigma(28)-dependent transcription of gene...

journal_title：Genes & development

authors： Aldridge PD,Karlinsey JE,Aldridge C,Birchall C,Thompson D,Yagasaki J,Hughes KT

更新日期：2006-08-15 00:00:00

abstract：:A key pathway that controls both cell division and differentiation in animal cells is mediated by the retinoblastoma (RB) family of tumor suppressors, which gate the passage of cells from G(1) to S and through S phase. The role(s) of the RB pathway in plants are not yet clearly defined, nor has there been any evidence...

journal_title：Genes & development

authors： Umen JG,Goodenough UW

更新日期：2001-07-01 00:00:00