Abstract:
:The sigma(28) protein is a member of the bacterial sigma(70)-family of transcription factors that directs RNA polymerase to flagellar late (class 3) promoters. The sigma(28) protein is regulated in response to flagellar assembly by the anti-sigma(28) factor FlgM. FlgM inhibits sigma(28)-dependent transcription of genes whose products are needed late in assembly until the flagellar basal motor structure, the hook-basal body (HBB), is constructed. A second function for the sigma(28) transcription factor has been discovered: sigma(28) facilitates the secretion of FlgM through the HBB, acting as the FlgM Type III secretion chaperone. Transcription-specific mutants in sigma(28) were isolated that remained competent for FlgM-facilitated secretion separating the transcription and secretion-facilitation activities of sigma (28). Conversely, we also describe the isolation of mutants in sigma(28) that are specific for FlgM-facilitated secretion. The data demonstrate that sigma(28) is the Type III secretion chaperone for its own anti-sigma factor FlgM. Thus, a novel role for a sigma(70)-family transcription factor is described.
journal_name
Genes Devjournal_title
Genes & developmentauthors
Aldridge PD,Karlinsey JE,Aldridge C,Birchall C,Thompson D,Yagasaki J,Hughes KTdoi
10.1101/gad.380406subject
Has Abstractpub_date
2006-08-15 00:00:00pages
2315-26issue
16eissn
0890-9369issn
1549-5477pii
20/16/2315journal_volume
20pub_type
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