当前位置： SCI文献检索 > GENES & DEVELOPMENT期刊下所有文献 > Nascent-seq indicates widespread cotranscriptional pre-mRNA splicing in Drosophila.

Nascent-seq indicates widespread cotranscriptional pre-mRNA splicing in Drosophila.

Abstract：

:To determine the prevalence of cotranscriptional splicing in Drosophila, we sequenced nascent RNA transcripts from Drosophila S2 cells as well as from Drosophila heads. Eighty-seven percent of the introns assayed manifest >50% cotranscriptional splicing. The remaining 13% are cotranscriptionally spliced poorly or slowly, with ∼3% being almost completely retained in nascent pre-mRNA. Although individual introns showed slight but statistically significant differences in splicing efficiency, similar global levels of splicing were seen from both sources. Importantly, introns with low cotranscriptional splicing efficiencies are present in the same primary transcript with efficiently spliced introns, indicating that splicing is intron-specific. The analysis also indicates that cotranscriptional splicing is less efficient for first introns, longer introns, and introns annotated as alternative. Finally, S2 cells expressing the slow RpII215(C4) mutant show substantially less intron retention than wild-type S2 cells.

journal_name

Genes Dev

journal_title

Genes & development

authors

Khodor YL,Rodriguez J,Abruzzi KC,Tang CH,Marr MT 2nd,Rosbash M

doi

10.1101/gad.178962.111

subject

Has Abstract

pub_date

2011-12-01 00:00:00

pages

2502-12

issue

23

eissn

0890-9369

issn

1549-5477

pii

25/23/2502

journal_volume

25

pub_type

杂志文章

相关文献

GENES & DEVELOPMENT文献大全
  • Transcription factor Spo0A switches the localization of the cell division protein FtsZ from a medial to a bipolar pattern in Bacillus subtilis.

    abstract：:Entry into sporulation by the Gram-positive bacterium Bacillus subtilis is governed by two transcription factors, Spo0A and sigma H, and involves a switch in the site of division from a medial to a polar location. We report that at the onset of sporulation, assembly of the cell division protein FtsZ shifts from midcel...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.10.4.478

    authors： Levin PA,Losick R

    更新日期：1996-02-15 00:00:00

  • Multipotent hematopoietic cells susceptible to alternative double-strand break repair pathways that promote genome rearrangements.

    abstract：:Chromosomal translocations are a hallmark of hematopoietic malignancies. The initial molecular events or pathways that lead to translocations in hematopoietic cells are largely unknown, particularly in the stem cell-enriched population postulated to be the initial target for these events. We used in vitro differentiat...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.1522807

    authors： Francis R,Richardson C

    更新日期：2007-05-01 00:00:00

  • Cyclin D3 coordinates the cell cycle during differentiation to regulate erythrocyte size and number.

    abstract：:Genome-wide association studies (GWASs) have identified a genetic variant of moderate effect size at 6p21.1 associated with erythrocyte traits in humans. We show that this variant affects an erythroid-specific enhancer of CCND3. A Ccnd3 knockout mouse phenocopies these erythroid phenotypes, with a dramatic increase in...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.197020.112

    authors： Sankaran VG,Ludwig LS,Sicinska E,Xu J,Bauer DE,Eng JC,Patterson HC,Metcalf RA,Natkunam Y,Orkin SH,Sicinski P,Lander ES,Lodish HF

    更新日期：2012-09-15 00:00:00

  • Reelin-induced tyrosine [corrected] phosphorylation of disabled 1 during neuronal positioning.

    abstract：:The reelin (reln) and disabled 1 (dab1) genes both ensure correct neuronal positioning during brain development. We have found that the intracellular Dab1 protein receives a tyrosine phosphorylation signal from extracellular Reln protein. Genetic analysis shows that reln function depends on dab1, and vice versa, as ex...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.13.6.643

    authors： Howell BW,Herrick TM,Cooper JA

    更新日期：1999-03-15 00:00:00

  • Control of cell division by a retinoblastoma protein homolog in Chlamydomonas.

    abstract：:A key pathway that controls both cell division and differentiation in animal cells is mediated by the retinoblastoma (RB) family of tumor suppressors, which gate the passage of cells from G(1) to S and through S phase. The role(s) of the RB pathway in plants are not yet clearly defined, nor has there been any evidence...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.892101

    authors： Umen JG,Goodenough UW

    更新日期：2001-07-01 00:00:00

  • Direct induction of G1-specific transcripts following reactivation of the Cdc28 kinase in the absence of de novo protein synthesis.

    abstract：:In Saccharomyces cerevisiae, the genes encoding the HO endonuclease, G1-specific cyclins CLN1 and CLN2, as well as most proteins involved in DNA synthesis, are periodically transcribed with maximal levels reached in late G1. For HO and the DNA replication genes, cell cycle stage-specific expression has been shown to b...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.6.4.557

    authors： Marini NJ,Reed SI

    更新日期：1992-04-01 00:00:00

  • Genomic imprinting in plants-revisiting existing models.

    abstract：:Genomic imprinting is an epigenetic phenomenon leading to parentally biased gene expression. Throughout the years, extensive efforts have been made to characterize the epigenetic marks underlying imprinting in animals and plants. As a result, DNA methylation asymmetries between parental genomes emerged as the primary ...

    journal_title：Genes & development

    pub_type： 杂志文章,评审

    doi：10.1101/gad.332924.119

    authors： Batista RA,Köhler C

    更新日期：2020-01-01 00:00:00

  • An ERK2 docking site in the Pointed domain distinguishes a subset of ETS transcription factors.

    abstract：:The ETS transcription factors perform distinct biological functions despite conserving a highly similar DNA-binding domain. One distinguishing property of a subset of ETS proteins is a conserved region of 80 amino acids termed the Pointed (PNT) domain. Using enzyme kinetics we determined that the Ets-1 PNT domain cont...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.950902

    authors： Seidel JJ,Graves BJ

    更新日期：2002-01-01 00:00:00

  • The kinase haspin is required for mitotic histone H3 Thr 3 phosphorylation and normal metaphase chromosome alignment.

    abstract：:Post-translational modifications of conserved N-terminal tail residues in histones regulate many aspects of chromosome activity. Thr 3 of histone H3 is highly conserved, but the significance of its phosphorylation is unclear, and the identity of the corresponding kinase unknown. Immunostaining with phospho-specific an...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.1267105

    authors： Dai J,Sultan S,Taylor SS,Higgins JM

    更新日期：2005-02-15 00:00:00

  • Transabdominal, a dominant mutant of the Bithorax Complex, produces a sexually dimorphic segmental transformation in Drosophila.

    abstract：:Transabdominal (Tab), a dominant mutation in the Bithorax Complex (BX-C) of Drosophila, creates a sexually dimorphic pattern of segmental transformation that has complete penetrance and expressivity. Specific regions within the notum of the second thoracic segment (T2) are transformed into abdominal-like cuticle; thus...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.1.2.111

    authors： Celniker SE,Lewis EB

    更新日期：1987-04-01 00:00:00

  • Cooperative recruitment of Yan via a high-affinity ETS supersite organizes repression to confer specificity and robustness to cardiac cell fate specification.

    abstract：:Cis-regulatory modules (CRMs) are defined by unique combinations of transcription factor-binding sites. Emerging evidence suggests that the number, affinity, and organization of sites play important roles in regulating enhancer output and, ultimately, gene expression. Here, we investigate how the cis-regulatory logic ...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.307132.117

    authors： Boisclair Lachance JF,Webber JL,Hong L,Dinner AR,Rebay I

    更新日期：2018-03-01 00:00:00

  • EGF domain swap converts a drosophila EGF receptor activator into an inhibitor.

    abstract：:In Drosophila the function of the epidermal growth factor (EGF) receptor is modulated zygotically by three EGF-like proteins: Spitz (Spi), which is a potent activator; Vein (Vn), which is a moderate activator; and Argos (Aos), which is an inhibitor. Chimeric molecules were constructed in which the EGF domain of Vn was...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.12.7.908

    authors： Schnepp B,Donaldson T,Grumbling G,Ostrowski S,Schweitzer R,Shilo BZ,Simcox A

    更新日期：1998-04-01 00:00:00

  • Methylation of RUNX1 by PRMT1 abrogates SIN3A binding and potentiates its transcriptional activity.

    abstract：:RUNX1/AML1 is required for the development of definitive hematopoiesis, and its activity is altered by mutations, deletions, and chromosome translocations in human acute leukemia. RUNX1 function can be regulated by post-translational modifications and protein-protein interactions. We show that RUNX1 is arginine-methyl...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.1632608

    authors： Zhao X,Jankovic V,Gural A,Huang G,Pardanani A,Menendez S,Zhang J,Dunne R,Xiao A,Erdjument-Bromage H,Allis CD,Tempst P,Nimer SD

    更新日期：2008-03-01 00:00:00

  • The bHLH gene Hes1 is essential for expansion of early T cell precursors.

    abstract：:Mice mutant for the bHLH gene Hes1, which is known to keep cells in a proliferative state, mostly lack thymus. Transfer of Hes1-null fetal liver cells into RAG2-null host mice normally reconstitutes B cells but fails to generate mature T cells in the thymus. In the reconstituted thymus, T cell differentiation is arres...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.13.9.1203

    authors： Tomita K,Hattori M,Nakamura E,Nakanishi S,Minato N,Kageyama R

    更新日期：1999-05-01 00:00:00

  • The Elongin BC complex interacts with the conserved SOCS-box motif present in members of the SOCS, ras, WD-40 repeat, and ankyrin repeat families.

    abstract：:The Elongin BC complex was identified initially as a positive regulator of RNA polymerase II (Pol II) elongation factor Elongin A and subsequently as a component of the multiprotein von Hippel-Lindau (VHL) tumor suppressor complex, in which it participates in both tumor suppression and negative regulation of hypoxia-i...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.12.24.3872

    authors： Kamura T,Sato S,Haque D,Liu L,Kaelin WG Jr,Conaway RC,Conaway JW

    更新日期：1998-12-15 00:00:00

  • Nuclear localization of the Arabidopsis APETALA3 and PISTILLATA homeotic gene products depends on their simultaneous expression.

    abstract：:The Arabidopsis APETALA3 (AP3) and PISTILLATA (PI) proteins are thought to act as transcription factors and are required for specifying floral organ identities. To define the nuclear localization signals within these proteins, we generated translational fusions of the coding regions of AP3 and PI to the bacterial uidA...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.10.14.1812

    authors： McGonigle B,Bouhidel K,Irish VF

    更新日期：1996-07-15 00:00:00

  • H3.3 actively marks enhancers and primes gene transcription via opening higher-ordered chromatin.

    abstract：:The histone variants H3.3 and H2A.Z have recently emerged as two of the most important features in transcriptional regulation, the molecular mechanism of which still remains poorly understood. In this study, we investigated the regulation of H3.3 and H2A.Z on chromatin dynamics during transcriptional activation. Our i...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.222174.113

    authors： Chen P,Zhao J,Wang Y,Wang M,Long H,Liang D,Huang L,Wen Z,Li W,Li X,Feng H,Zhao H,Zhu P,Li M,Wang QF,Li G

    更新日期：2013-10-01 00:00:00

  • Terminal differentiation of myelin-forming oligodendrocytes depends on the transcription factor Sox10.

    abstract：:Sox10 is a high-mobility-group transcriptional regulator in early neural crest. Without Sox10, no glia develop throughout the peripheral nervous system. Here we show that Sox10 is restricted in the central nervous system to myelin-forming oligodendroglia. In Sox10-deficient mice progenitors develop, but terminal diffe...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.215802

    authors： Stolt CC,Rehberg S,Ader M,Lommes P,Riethmacher D,Schachner M,Bartsch U,Wegner M

    更新日期：2002-01-15 00:00:00

  • SMG6 interacts with the exon junction complex via two conserved EJC-binding motifs (EBMs) required for nonsense-mediated mRNA decay.

    abstract：:Nonsense-mediated mRNA decay (NMD) is a quality control mechanism that detects and degrades mRNAs containing premature stop codons (PTCs). In vertebrates, PTCs trigger efficient NMD when located upstream of an exon junction complex (EJC). Degradation of PTC-containing mRNAs requires the endonucleolytic activity of SMG...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.604610

    authors： Kashima I,Jonas S,Jayachandran U,Buchwald G,Conti E,Lupas AN,Izaurralde E

    更新日期：2010-11-01 00:00:00

  • Differential requirements for cell-specific elastase I enhancer domains in transfected cells and transgenic mice.

    abstract：:The 134-bp enhancer region of the pancreatic elastase I gene is sufficient to direct pancreatic acinar cell-specific transcription in transgenic mice and in transfected cells in culture. Ten-base-pair scanner mutations in three separate enhancer domains that inactivate enhancer function in transfected pancreatic cells...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.3.5.687

    authors： Swift GH,Kruse F,MacDonald RJ,Hammer RE

    更新日期：1989-05-01 00:00:00

  • Genome integrity and disease prevention in the nervous system.

    abstract：:Multiple DNA repair pathways maintain genome stability and ensure that DNA remains essentially unchanged over the life of a cell. Various human diseases occur if DNA repair is compromised, and most of these impact the nervous system, in some cases exclusively. However, it is often unclear what specific endogenous dama...

    journal_title：Genes & development

    pub_type： 杂志文章,评审

    doi：10.1101/gad.301325.117

    authors： McKinnon PJ

    更新日期：2017-06-15 00:00:00

  • Increased Rrm2 gene dosage reduces fragile site breakage and prolongs survival of ATR mutant mice.

    abstract：:In Saccharomyces cerevisiae, absence of the checkpoint kinase Mec1 (ATR) is viable upon mutations that increase the activity of the ribonucleotide reductase (RNR) complex. Whether this pathway is conserved in mammals remains unknown. Here we show that cells from mice carrying extra alleles of the RNR regulatory subuni...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.256958.114

    authors： Lopez-Contreras AJ,Specks J,Barlow JH,Ambrogio C,Desler C,Vikingsson S,Rodrigo-Perez S,Green H,Rasmussen LJ,Murga M,Nussenzweig A,Fernandez-Capetillo O

    更新日期：2015-04-01 00:00:00

  • Targeted disruption of an essential vertebrate gene: ASF/SF2 is required for cell viability.

    abstract：:Alternative splicing factor/splicing factor 2 (ASF/SF2) is the prototype of a family of nuclear proteins highly conserved throughout metazoa, the SR (serine/arginine) proteins. Based largely on in vitro studies, SR proteins have been suggested to play important roles in constitutive and alternative splicing of pre-mRN...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.10.20.2588

    authors： Wang J,Takagaki Y,Manley JL

    更新日期：1996-10-15 00:00:00

  • The PRC1 Polycomb group complex interacts with PLZF/RARA to mediate leukemic transformation.

    abstract：:Ectopic repression of retinoic acid (RA) receptor target genes by PML/RARA and PLZF/RARA fusion proteins through aberrant recruitment of nuclear corepressor complexes drives cellular transformation and acute promyelocytic leukemia (APL) development. In the case of PML/RARA, this repression can be reversed through trea...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.512009

    authors： Boukarabila H,Saurin AJ,Batsché E,Mossadegh N,van Lohuizen M,Otte AP,Pradel J,Muchardt C,Sieweke M,Duprez E

    更新日期：2009-05-15 00:00:00

  • Apoptosis or retinoblastoma: alternative fates of photoreceptors expressing the HPV-16 E7 gene in the presence or absence of p53.

    abstract：:A transgenic mouse model for retinoblastoma was produced previously by directing SV40 T antigen expression to retinal photoreceptor cells using the promoter of the interstitial retinol-binding protein (IRBP) gene. This gene becomes active prior to the terminal differentiation of photoreceptors. Because T antigen-trans...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.8.11.1300

    authors： Howes KA,Ransom N,Papermaster DS,Lasudry JG,Albert DM,Windle JJ

    更新日期：1994-06-01 00:00:00

  • Stimulation of transcription by an Ultrabithorax protein in vitro.

    abstract：:The Ultrabithorax (Ubx) gene of Drosophila melanogaster encodes a family of UBX proteins that are thought to specify the developmental fates of segments in the posterior thorax and anterior abdomen by controlling the expression of a set of target genes. UBX proteins bind DNA in vitro, and they activate or repress diff...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.4.6.1044

    authors： Johnson FB,Krasnow MA

    更新日期：1990-06-01 00:00:00

  • Sonic hedgehog acts cell-autonomously on muscle precursor cells to generate limb muscle diversity.

    abstract：:How muscle diversity is generated in the vertebrate body is poorly understood. In the limb, dorsal and ventral muscle masses constitute the first myogenic diversification, as each gives rise to distinct muscles. Myogenesis initiates after muscle precursor cells (MPCs) have migrated from the somites to the limb bud and...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.187807.112

    authors： Anderson C,Williams VC,Moyon B,Daubas P,Tajbakhsh S,Buckingham ME,Shiroishi T,Hughes SM,Borycki AG

    更新日期：2012-09-15 00:00:00

  • A monocarboxylate transporter required for hepatocyte secretion of ketone bodies during fasting.

    abstract：:To find new genes that influence liver lipid mass, we performed a genetic screen for zebrafish mutants with hepatic steatosis, a pathological accumulation of fat. The red moon (rmn) mutant develops hepatic steatosis as maternally deposited yolk is depleted. Conversely, hepatic steatosis is suppressed in rmn mutants by...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.180968.111

    authors： Hugo SE,Cruz-Garcia L,Karanth S,Anderson RM,Stainier DY,Schlegel A

    更新日期：2012-02-01 00:00:00

  • GCN2 sustains mTORC1 suppression upon amino acid deprivation by inducing Sestrin2.

    abstract：:Mammalian cells possess two amino acid-sensing kinases: general control nonderepressible 2 (GCN2) and mechanistic target of rapamycin complex 1 (mTORC1). Their combined effects orchestrate cellular adaptation to amino acid levels, but how their activities are coordinated remains poorly understood. Here, we demonstrate...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.269324.115

    authors： Ye J,Palm W,Peng M,King B,Lindsten T,Li MO,Koumenis C,Thompson CB

    更新日期：2015-11-15 00:00:00

  • Novel Cdc42-binding proteins Gic1 and Gic2 control cell polarity in yeast.

    abstract：:Cdc42p, a Rho-related GTP-binding protein, regulates cytoskeletal polarization and rearrangements in eukaryotic cells, but the effectors mediating this control remain unknown. Through the use of the complete yeast genomic sequence, we have identified two novel Cdc42p targets, Gic1p and Gic2p, which contain consensus C...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.11.22.2972

    authors： Brown JL,Jaquenoud M,Gulli MP,Chant J,Peter M

    更新日期：1997-11-15 00:00:00