Abstract:
:To understand how chromosome shapes are determined by actions of condensins and cohesin, we devised a series of protocols in which their levels are precisely changed in Xenopus egg extracts. When the relative ratio of condensin I to II is forced to be smaller, embryonic chromosomes become shorter and thicker, being reminiscent of somatic chromosomes. Further depletion of condensin II unveils its contribution to axial shortening of chromosomes. Cohesin helps juxtapose sister chromatid arms by collaborating with condensin I and counteracting condensin II. Thus, chromosome shaping is achieved by an exquisite balance among condensin I and II and cohesin.
journal_name
Genes Devjournal_title
Genes & developmentauthors
Shintomi K,Hirano Tdoi
10.1101/gad.2060311subject
Has Abstractpub_date
2011-07-15 00:00:00pages
1464-9issue
14eissn
0890-9369issn
1549-5477pii
gad.2060311journal_volume
25pub_type
杂志文章abstract::The transformer-2 (tra-2) gene of Drosophila melanogaster plays essential roles in both sexual differentiation in the female soma and spermatogenesis in the male germ line. In the female soma, tra-2 is known to act with other genes in the sex determination regulatory cascade to control the sex-specific alternative spl...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.4.5.789
更新日期:1990-05-01 00:00:00
abstract::The ability to maintain and expand the pool of adipocytes in adults is integral to the regulation of energy balance, tissue/stem cell homeostasis, and disease pathogenesis. For decades, our knowledge of adipocyte precursors has relied on cellular models. The identity of native adipocyte precursors has remained unclear...
journal_title:Genes & development
pub_type: 杂志文章,评审
doi:10.1101/gad.293704.116
更新日期:2017-01-15 00:00:00
abstract::Lipids play crucial roles in many aspects of glial cell biology, affecting processes ranging from myelin membrane biosynthesis to axo-glial interactions. In order to study the role of lipid metabolism in myelinating glial cells, we specifically deleted in Schwann cells the Lpin1 gene, which encodes the Mg2+-dependent ...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.1638008
更新日期:2008-06-15 00:00:00
abstract::Gastrulation in mice is associated with the start of extreme proliferation and differentiation. The potential cost to the embryo of a very rapid proliferation rate is a high production of damaged cells. We demonstrate a novel surveillance mechanism for the elimination of cells damaged by ionizing radiation during mous...
journal_title:Genes & development
pub_type: 杂志文章
doi:
更新日期:2000-08-15 00:00:00
abstract::The assembly of activated RNA polymerase II (pol II) transcription complexes has been investigated by assaying whether pre-assembly of intermediate complexes reduces the extended time required for start-site melting. The results show that a closed complex requiring factors IIA, IID, and the acidic activator GAL4-AH fo...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.6.9.1716
更新日期:1992-09-01 00:00:00
abstract::The efficiency of RNA 3'-end formation of spleen necrosis virus (SNV) is determined by the distance between the cap site and the poly(A) site. When the distance between the cap site and the poly(A) site was shorter than 500 bases, only 3-9% of the RNA was polyadenylated at the SNV poly(A) site. However, when the dista...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.4.12b.2299
更新日期:1990-12-01 00:00:00
abstract::While microRNAs (miRNAs) regulate the vast majority of protein-encoding transcripts, little is known about how miRNAs themselves are degraded. We recently described Tudor-staphylococcal/micrococcal-like nuclease (TSN)-mediated miRNA decay (TumiD) as a cellular pathway in which the nuclease TSN promotes the decay of mi...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.303537.117
更新日期:2017-07-15 00:00:00
abstract::In Saccharomyces cerevisiae, the genes encoding the HO endonuclease, G1-specific cyclins CLN1 and CLN2, as well as most proteins involved in DNA synthesis, are periodically transcribed with maximal levels reached in late G1. For HO and the DNA replication genes, cell cycle stage-specific expression has been shown to b...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.6.4.557
更新日期:1992-04-01 00:00:00
abstract::The amino-terminal histone tails are subject to covalent post-translational modifications such as acetylation, methylation, and phosphorylation. In the histone code hypothesis, these exposed and unstructured histone tails are accessible to a repertoire of regulatory factors that specifically recognize the various modi...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.1001502
更新日期:2002-06-15 00:00:00
abstract::The transcription factor NF-kappaB is a critical regulator of inflammatory and cell survival signals. Proteasomal degradation of NF-kappaB subunits plays an important role in the termination of NF-kappaB activity, and at least one of the identified ubiquitin ligases is a multimeric complex containing Copper Metabolism...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.1748409
更新日期:2009-04-01 00:00:00
abstract::Piwi-piRNA (Piwi-interacting RNA) ribonucleoproteins (piRNPs) enforce retrotransposon silencing, a function critical for preserving the genome integrity of germ cells. The molecular functions of most of the factors that have been genetically implicated in primary piRNA biogenesis are still elusive. Here we show that M...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.254631.114
更新日期:2015-03-15 00:00:00
abstract::In recent years several new mouse models for lung cancer have been described. These include models for both non-small-cell lung cancer (NSCLC) and small-cell lung cancer (SCLC). Tumorigenesis in these conditional mouse tumor models can be initiated in adult mice through Cre-recombinase-induced activation of oncogenic ...
journal_title:Genes & development
pub_type: 杂志文章,评审
doi:10.1101/gad.1284505
更新日期:2005-03-15 00:00:00
abstract::Cyclin B3 has been conserved during higher eukaryote evolution as evidenced by its identification in chicken, nematodes, and insects. We demonstrate that Cyclin B3 is present in addition to Cyclins A and B in mitotically proliferating cells and not detectable in endoreduplicating tissues of Drosophila embryos. Cyclin ...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.12.23.3741
更新日期:1998-12-01 00:00:00
abstract::The Notch signaling pathway is essential for embryonic vascular development in vertebrates. Here we show that mouse embryos heterozygous for a targeted mutation in the gene encoding the DLL4 ligand exhibit haploinsufficient lethality because of defects in vascular remodeling. We also describe vascular defects in embry...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.1239204
更新日期:2004-10-15 00:00:00
abstract::NF-E1, a DNA-binding protein that recognizes the general consensus motif WGATAR, is the first tissue-specific factor to be identified in erythroid cells. Using a probe from the murine GF-1 (NF-E1) cDNA clone, we isolated three homologous chicken cDNAs: One of these corresponds to an mRNA (NF-E1a) that is abundantly an...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.4.10.1650
更新日期:1990-10-01 00:00:00
abstract::The progressive bone marrow failure syndrome dyskeratosis congenita (DC) is often caused by mutations in telomerase or the factors involved in telomerase biogenesis and trafficking. However, a subset of DC patients is heterozygous for mutations in the shelterin component TIN2. To determine how the TIN2-DC mutations af...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.233395.113
更新日期:2014-01-15 00:00:00
abstract::Exposure of hematopoietic progenitors to gamma-irradiation (IR) induces p53-dependent apoptosis and a p53-independent G2/M cell cycle arrest. These responses to DNA-damage can be inhibited by treatment with cytokine growth factors. Here we report that gamma-IR-induced apoptosis and cell cycle arrest are suppressed by ...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.12.8.1099
更新日期:1998-04-15 00:00:00
abstract::Nonsense-mediated mRNA decay (NMD) is a quality control mechanism that detects and degrades mRNAs containing premature stop codons (PTCs). In vertebrates, PTCs trigger efficient NMD when located upstream of an exon junction complex (EJC). Degradation of PTC-containing mRNAs requires the endonucleolytic activity of SMG...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.604610
更新日期:2010-11-01 00:00:00
abstract::H4K20 methylation is a broad chromatin modification that has been linked with diverse epigenetic functions. Several enzymes target H4K20 methylation, consistent with distinct mono-, di-, and trimethylation states controlling different biological outputs. To analyze the roles of H4K20 methylation states, we generated c...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.476008
更新日期:2008-08-01 00:00:00
abstract::The gene for an essential protein subunit of nuclear RNase P from Saccharomyces cerevisiae has been cloned. The gene for this protein, RPP1, was identified by virtue of its homology with a human scleroderma autoimmune antigen, Rpp30, which copurifies with human RNase P. Epitope-tagged Rpp1 can be found in association ...
journal_title:Genes & development
pub_type: 更正并重新发布的文章,杂志文章
doi:10.1101/gad.11.21.2926
更新日期:1997-11-01 00:00:00
abstract::The secreted Drosophila Hedgehog (Hh) protein induces transcription of specific genes by an unknown mechanism that requires the serpentine transmembrane protein Smoothened (Smo) and the transcription factor Cubitus interruptus (Ci). Protein kinase A (PKA) has been implicated in the mechanism of Hh signal transduction ...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.11.17.2250
更新日期:1997-09-01 00:00:00
abstract::The subdivision of the Drosophila body into distinct terminal and central domains depends on the torso (tor) protein, a putative receptor tyrosine kinase that is active at both ends of the early embryo. We show that the tor protein is uniformly expressed along the surface membrane of early embryos despite its localize...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.3.12b.2025
更新日期:1989-12-01 00:00:00
abstract::Cells have evolved checkpoint responses to arrest or delay the cell cycle, activate DNA repair networks, or induce apoptosis after genomic perturbation. Cells have also evolved the translesion synthesis processes to tolerate genomic lesions by either error-free or error-prone repair. Here, we show that after a replica...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.1043203
更新日期:2003-01-01 00:00:00
abstract::The mammalian high mobility group proteins HMG1 and HMG2 are abundant, chromatin-associated proteins whose cellular function is not known. In this study we show that these proteins can substitute for the prokaryotic DNA-bending protein HU in promoting the assembly of the Hin invertasome, an intermediate structure in H...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.7.8.1521
更新日期:1993-08-01 00:00:00
abstract::The assembly and disassembly of protein complexes at replication origins play a crucial role in the regulation of chromosomal DNA replication. The sequential binding of the origin recognition complex (ORC), Cdc6, and the minichromosome maintenance (MCM/P1) proteins produces a licensed replication origin. Before the in...
journal_title:Genes & development
pub_type: 杂志文章
doi:
更新日期:2000-06-15 00:00:00
abstract::The incorporation of histone H1 into chromatin during embryogenesis directs the specific repression of the Xenopus oocyte 5S rRNA genes. An increase in histone H1 content specifically restricts TFIIIA-activated transcription, and a decrease in histone H1 within chromatin facilitates the activation of the oocyte 5S rRN...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.8.10.1147
更新日期:1994-05-15 00:00:00
abstract::SpoIIIE is an FtsK-related protein that transports the forespore chromosome across the Bacillus subtilis sporulation septum. We use membrane photobleaching and protoplast assays to demonstrate that SpoIIIE is required for septal membrane fission in the presence of trapped DNA, and that DNA is transported across separa...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.1925210
更新日期:2010-06-01 00:00:00
abstract::YAP (Yes-associated protein) and TAZ (transcriptional coactivator with PDZ-binding motif) are major downstream effectors of the Hippo pathway that influences tissue homeostasis, organ size, and cancer development. Aberrant hyperactivation of YAP/TAZ causes tissue overgrowth and tumorigenesis, whereas their inactivatio...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.262816.115
更新日期:2015-06-15 00:00:00
abstract::The td, nrdB, and sunY introns of bacteriophage T4 each contain a long open reading frame (ORF). These ORFs are preceded by functional T4 late promoters and, in the case of the nrdB intron ORF, a functional middle promoter. Expression of phage-encoded intron ORF-lacZ fusions indicates that these T4 genes are highly re...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.2.12b.1791
更新日期:1988-12-01 00:00:00
abstract::Disruptions in the use of skeletal muscle lead to muscle atrophy. After short periods of disuse, muscle atrophy is reversible, and even after prolonged periods of inactivity, myofiber degeneration is uncommon. The pathways that regulate atrophy, initiated either by peripheral nerve damage, immobilization, aging, catab...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.1318305
更新日期:2005-07-15 00:00:00