当前位置： SCI文献检索 > GENES & DEVELOPMENT期刊下所有文献 > CHD4/Mi-2beta activity is required for the positioning of the mesoderm/neuroectoderm boundary in Xenopus.

CHD4/Mi-2beta activity is required for the positioning of the mesoderm/neuroectoderm boundary in Xenopus.

Abstract：

:Experiments in Xenopus have illustrated the importance of extracellular morphogens for embryonic gene regulation in vertebrates. Much less is known about how induction leads to the correct positioning of boundaries; for example, between germ layers. Here we report that the neuroectoderm/mesoderm boundary is controlled by the chromatin remodeling ATPase CHD4/Mi-2beta. Gain and loss of CHD4 function experiments shifted this boundary along the animal-vegetal axis at gastrulation, leading to excess mesoderm formation at the expense of neuroectoderm, or vice versa. This phenotype results from specific alterations in gene transcription, notably of the neural-promoting gene Sip1 and the mesodermal regulatory gene Xbra. We show that CHD4 suppresses Sip1 transcription by direct binding to the 5' end of the Sip1 gene body. Furthermore, we demonstrate that CHD4 and Sip1 expression levels determine the "ON" threshold for Nodal-dependent but not for eFGF-dependent induction of Xbra transcription. The CHD4/Sip1 epistasis thus constitutes a regulatory module, which balances mesoderm and neuroectoderm formation.

journal_name

Genes Dev

journal_title

Genes & development

authors

Linder B,Mentele E,Mansperger K,Straub T,Kremmer E,Rupp RA

doi

10.1101/gad.409507

subject

Has Abstract

pub_date

2007-04-15 00:00:00

pages

973-83

issue

8

eissn

0890-9369

issn

1549-5477

pii

21/8/973

journal_volume

21

pub_type

杂志文章

相关文献

GENES & DEVELOPMENT文献大全
  • SAM68 is required for regulation of Pumilio by the NORAD long noncoding RNA.

    abstract：:The number of known long noncoding RNA (lncRNA) functions is rapidly growing, but how those functions are encoded in their sequence and structure remains poorly understood. NORAD (noncoding RNA activated by DNA damage) is a recently characterized, abundant, and highly conserved lncRNA that is required for proper mitot...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.309138.117

    authors： Tichon A,Perry RB,Stojic L,Ulitsky I

    更新日期：2018-01-01 00:00:00

  • A conserved protein network controls assembly of the outer kinetochore and its ability to sustain tension.

    abstract：:Kinetochores play an essential role in chromosome segregation by forming dynamic connections with spindle microtubules. Here, we identify a set of 10 copurifying kinetochore proteins from Caenorhabditis elegans, seven of which were previously uncharacterized. Using in vivo assays to monitor chromosome segregation, kin...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.1234104

    authors： Cheeseman IM,Niessen S,Anderson S,Hyndman F,Yates JR 3rd,Oegema K,Desai A

    更新日期：2004-09-15 00:00:00

  • The interplay between multiple enhancer and silencer elements defines the pattern of decapentaplegic expression.

    abstract：:The product of the zygotically active decapentaplegic (dpp) gene appears to function as a morphogen that specifies positional information in the dorsal half of the Drosophila embryo. The dorsal-specific transcription of dpp is the key step in establishing a morphogen gradient. We demonstrate here that multiple regions...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.7.4.694

    authors： Huang JD,Schwyter DH,Shirokawa JM,Courey AJ

    更新日期：1993-04-01 00:00:00

  • Yeast calcineurin regulates nuclear localization of the Crz1p transcription factor through dephosphorylation.

    abstract：:Calcineurin, a Ca2+/calmodulin dependent protein phosphatase, regulates Ca2+-dependent processes in a wide variety of cells. In the yeast, Saccharomyces cerevisiae, calcineurin effects Ca2+-dependent changes in gene expression through regulation of the Crz1p transcription factor. We show here that calcineurin dephosph...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.13.7.798

    authors： Stathopoulos-Gerontides A,Guo JJ,Cyert MS

    更新日期：1999-04-01 00:00:00

  • Gene encoding a morphogenic protein required in the assembly of the outer coat of the Bacillus subtilis endospore.

    abstract：:Endospores of Bacillus subtilis are encased in a two-layer protein shell known as the coat, which consists of a lammellar-like inner layer and an electron-dense outer layer. We report the cloning of the structural gene (designated cotE) for an alkali-soluble coat protein of 24 kD and show that the cotE gene product is...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.2.8.1047

    authors： Zheng LB,Donovan WP,Fitz-James PC,Losick R

    更新日期：1988-08-01 00:00:00

  • Structural basis for distinct roles of SMAD2 and SMAD3 in FOXH1 pioneer-directed TGF-β signaling.

    abstract：:TGF-β receptors phosphorylate SMAD2 and SMAD3 transcription factors, which then form heterotrimeric complexes with SMAD4 and cooperate with context-specific transcription factors to activate target genes. Here we provide biochemical and structural evidence showing that binding of SMAD2 to DNA depends on the conformati...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.330837.119

    authors： Aragón E,Wang Q,Zou Y,Morgani SM,Ruiz L,Kaczmarska Z,Su J,Torner C,Tian L,Hu J,Shu W,Agrawal S,Gomes T,Márquez JA,Hadjantonakis AK,Macias MJ,Massagué J

    更新日期：2019-11-01 00:00:00

  • Frequent provirus insertional mutagenesis of Notch1 in thymomas of MMTVD/myc transgenic mice suggests a collaboration of c-myc and Notch1 for oncogenesis.

    abstract：:The MMTVD/myc transgenic mice spontaneously develop oligoclonal CD4+CD8+ T-cell tumors. We used provirus insertional mutagenesis in these mice to identify putative collaborators of c-myc. We found that Notch1 was mutated in a high proportion (52%) of these tumors. Proviruses were inserted upstream of the exon coding f...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.10.15.1930

    authors： Girard L,Hanna Z,Beaulieu N,Hoemann CD,Simard C,Kozak CA,Jolicoeur P

    更新日期：1996-08-01 00:00:00

  • TopBP1 activates ATR through ATRIP and a PIKK regulatory domain.

    abstract：:The ATR (ATM and Rad3-related) kinase and its regulatory partner ATRIP (ATR-interacting protein) coordinate checkpoint responses to DNA damage and replication stress. TopBP1 functions as a general activator of ATR. However, the mechanism by which TopBP1 activates ATR is unknown. Here, we show that ATRIP contains a Top...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.1666208

    authors： Mordes DA,Glick GG,Zhao R,Cortez D

    更新日期：2008-06-01 00:00:00

  • P-element-mediated enhancer detection: a versatile method to study development in Drosophila.

    abstract：:We generated and characterized greater than 500 Drosophila strains that carry single copies of a novel P-element enhancer detector. In the majority of the strains, the beta-galactosidase reporter gene in the P-transposon responds to nearby transcriptional regulatory sequences in the genome. A remarkable diversity of s...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.3.9.1288

    authors： Bellen HJ,O'Kane CJ,Wilson C,Grossniklaus U,Pearson RK,Gehring WJ

    更新日期：1989-09-01 00:00:00

  • Reciprocal activation of xenobiotic response genes by nuclear receptors SXR/PXR and CAR.

    abstract：:The cytochrome P450 (CYP) gene products such as CYP3A and CYP2B are essential for the metabolism of steroid hormones and xenochemicals including prescription drugs. Nuclear receptor SXR/PXR (steroid and xenobiotic receptor/pregnenolone X receptor) has been shown both biochemically and genetically to activate CYP3A gen...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.846800

    authors： Xie W,Barwick JL,Simon CM,Pierce AM,Safe S,Blumberg B,Guzelian PS,Evans RM

    更新日期：2000-12-01 00:00:00

  • A six-amino-acid motif is a major determinant in functional evolution of HOX1 proteins.

    abstract：:Gene duplication and divergence is a major driver in the emergence of evolutionary novelties. How variations in amino acid sequences lead to loss of ancestral activity and functional diversification of proteins is poorly understood. We used cross-species functional analysis of Drosophila Labial and its mouse HOX1 orth...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.342329.120

    authors： Singh NP,De Kumar B,Paulson A,Parrish ME,Zhang Y,Florens L,Conaway JW,Si K,Krumlauf R

    更新日期：2020-12-01 00:00:00

  • Neuroectoderm in Drosophila embryos is dependent on the mesoderm for positioning but not for formation.

    abstract：:By studying neuroectoderm formation in the absence of mesoderm and mesectoderm in mutants of the zygotic genes snail and twist, we have found that the number of neuroblasts is not reduced in these mutants, suggesting that mesoderm and mesectoderm are not essential for the initiation of neural development. The position...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.5.9.1577

    authors： Rao Y,Vaessin H,Jan LY,Jan YN

    更新日期：1991-09-01 00:00:00

  • The HIV-1 Tat protein activates transcription from an upstream DNA-binding site: implications for Tat function.

    abstract：:The Tat protein of human immunodeficiency virus type 1 (HIV-1) activates transcription following binding to nascent trans-activation response (TAR) RNA downstream of the transcription start site. Because Tat functions when bound to RNA, and in a position-dependent manner, it has been proposed that Tat works by a novel...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.5.12b.2496

    authors： Southgate CD,Green MR

    更新日期：1991-12-01 00:00:00

  • A genetic screen identifies the Triple T complex required for DNA damage signaling and ATM and ATR stability.

    abstract：:In response to DNA damage, cells activate a complex signal transduction network called the DNA damage response (DDR). To enhance our current understanding of the DDR network, we performed a genome-wide RNAi screen to identify genes required for resistance to ionizing radiation (IR). Along with a number of known DDR ge...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.1934210

    authors： Hurov KE,Cotta-Ramusino C,Elledge SJ

    更新日期：2010-09-01 00:00:00

  • The Rho-linked mental retardation protein oligophrenin-1 controls synapse maturation and plasticity by stabilizing AMPA receptors.

    abstract：:Oligophrenin-1 (OPHN1) encodes a Rho-GTPase-activating protein (Rho-GAP) whose loss of function has been associated with X-linked mental retardation (MR). The pathophysiological role of OPHN1, however, remains poorly understood. Here we show that OPHN1 through its Rho-GAP activity plays a critical role in the activity...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.1783809

    authors： Nadif Kasri N,Nakano-Kobayashi A,Malinow R,Li B,Van Aelst L

    更新日期：2009-06-01 00:00:00

  • Cyclin D3 coordinates the cell cycle during differentiation to regulate erythrocyte size and number.

    abstract：:Genome-wide association studies (GWASs) have identified a genetic variant of moderate effect size at 6p21.1 associated with erythrocyte traits in humans. We show that this variant affects an erythroid-specific enhancer of CCND3. A Ccnd3 knockout mouse phenocopies these erythroid phenotypes, with a dramatic increase in...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.197020.112

    authors： Sankaran VG,Ludwig LS,Sicinska E,Xu J,Bauer DE,Eng JC,Patterson HC,Metcalf RA,Natkunam Y,Orkin SH,Sicinski P,Lander ES,Lodish HF

    更新日期：2012-09-15 00:00:00

  • Rad6 plays a role in transcriptional activation through ubiquitylation of histone H2B.

    abstract：:Covalent modifications of the histone N tails play important roles in eukaryotic gene expression. Histone acetylation, in particular, is required for the activation of a subset of eukaryotic genes through the targeted recruitment of histone acetyltransferases. We have reported that a histone C tail modification, ubiqu...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.1149604

    authors： Kao CF,Hillyer C,Tsukuda T,Henry K,Berger S,Osley MA

    更新日期：2004-01-15 00:00:00

  • Analysis of a noncanonical poly(A) site reveals a tripartite mechanism for vertebrate poly(A) site recognition.

    abstract：:At least half of all human pre-mRNAs are subject to alternative 3' processing that may modulate both the coding capacity of the message and the array of post-transcriptional regulatory elements embedded within the 3' UTR. Vertebrate poly(A) site selection appears to rely primarily on the binding of CPSF to an A(A/U)UA...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.1298605

    authors： Venkataraman K,Brown KM,Gilmartin GM

    更新日期：2005-06-01 00:00:00

  • Regulation of plant innate immunity by three proteins in a complex conserved across the plant and animal kingdoms.

    abstract：:Innate immunity against pathogen infection is an evolutionarily conserved process among multicellular organisms. Arabidopsis SNC1 encodes a Resistance protein that combines attributes of multiple mammalian pattern recognition receptors. Utilizing snc1 as an autoimmune model, we identified a discrete protein complex co...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.1559607

    authors： Palma K,Zhao Q,Cheng YT,Bi D,Monaghan J,Cheng W,Zhang Y,Li X

    更新日期：2007-06-15 00:00:00

  • Dual pathways for induction of wingless expression by protein kinase A and Hedgehog in Drosophila embryos.

    abstract：:The secreted Drosophila Hedgehog (Hh) protein induces transcription of specific genes by an unknown mechanism that requires the serpentine transmembrane protein Smoothened (Smo) and the transcription factor Cubitus interruptus (Ci). Protein kinase A (PKA) has been implicated in the mechanism of Hh signal transduction ...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.11.17.2250

    authors： Ohlmeyer JT,Kalderon D

    更新日期：1997-09-01 00:00:00

  • Defects in Saccharomyces cerevisiae protein phosphatase type I activate the spindle/kinetochore checkpoint.

    abstract：:A conditional allele of type 1 protein phosphatase (glc7-129) in Saccharomyces cerevisiae causes first cycle arrest in G2/M, characterized by cells with a short spindle and high H1 kinase activity. Point-of-execution experiments indicate Glc7p function is required in G2/M just before anaphase for the completion of mit...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.13.5.517

    authors： Bloecher A,Tatchell K

    更新日期：1999-03-01 00:00:00

  • Radical mutations reveal TATA-box binding protein surfaces required for activated transcription in vivo.

    abstract：:Regions on the surface of human TATA-box binding protein (TBP) required for activated transcription in vivo were defined by construction of a library of 89 surface residue mutants with radical substitutions that were assayed for their ability to support activated transcription in vivo, basal transcription in vitro, an...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.10.19.2491

    authors： Bryant GO,Martel LS,Burley SK,Berk AJ

    更新日期：1996-10-01 00:00:00

  • A monocarboxylate transporter required for hepatocyte secretion of ketone bodies during fasting.

    abstract：:To find new genes that influence liver lipid mass, we performed a genetic screen for zebrafish mutants with hepatic steatosis, a pathological accumulation of fat. The red moon (rmn) mutant develops hepatic steatosis as maternally deposited yolk is depleted. Conversely, hepatic steatosis is suppressed in rmn mutants by...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.180968.111

    authors： Hugo SE,Cruz-Garcia L,Karanth S,Anderson RM,Stainier DY,Schlegel A

    更新日期：2012-02-01 00:00:00

  • Linking H3K79 trimethylation to Wnt signaling through a novel Dot1-containing complex (DotCom).

    abstract：:Epigenetic modifications of chromatin play an important role in the regulation of gene expression. KMT4/Dot1 is a conserved histone methyltransferase capable of methylating chromatin on Lys79 of histone H3 (H3K79). Here we report the identification of a multisubunit Dot1 complex (DotCom), which includes several of the...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.1898410

    authors： Mohan M,Herz HM,Takahashi YH,Lin C,Lai KC,Zhang Y,Washburn MP,Florens L,Shilatifard A

    更新日期：2010-03-15 00:00:00

  • Zeppo1 is a novel metastasis promoter that represses E-cadherin expression and regulates p120-catenin isoform expression and localization.

    abstract：:Amplification of 8p11-12 in human breast cancers is associated with increased proliferation and tumor grade and reduced metastasis-free patient survival. We identified Zeppo1 (zinc finger elbow-related proline domain protein 1) (FLJ14299/ZNF703) within this amplicon as a regulator of cell adhesion, migration, and prol...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.1998111

    authors： Slorach EM,Chou J,Werb Z

    更新日期：2011-03-01 00:00:00

  • The incidental pore: CaV1.2 and stem cell activation in quiescent hair follicles.

    abstract：:The hair follicle undergoes a lifelong developmental cycle that depends on the integration between activating and inhibitory signals acting to regulate and guide the proliferation and differentiation of pluripotent epithelial stem cells. The effectors and mechanisms responsible for re-entry of quiescent telogen hair f...

    journal_title：Genes & development

    pub_type： 评论,杂志文章

    doi：10.1101/gad.223172.113

    authors： Coulombe PA,Caterina MJ

    更新日期：2013-06-15 00:00:00

  • Integrin-linked kinase (ILK) is required for polarizing the epiblast, cell adhesion, and controlling actin accumulation.

    abstract：:Integrin-mediated cell-matrix interactions are essential for development, tissue homeostasis, and repair. Upon ligand binding, integrins are recruited into focal adhesions (FAs). Integrin-linked kinase (ILK) is an FA component that interacts with the cytoplasmic domains of integrins, recruits adaptor proteins that lin...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.255603

    authors： Sakai T,Li S,Docheva D,Grashoff C,Sakai K,Kostka G,Braun A,Pfeifer A,Yurchenco PD,Fässler R

    更新日期：2003-04-01 00:00:00

  • A novel homeobox gene mediates the Dpp signal to establish functional specificity within target cells.

    abstract：:Morphogen gradients of secreted molecules play critical roles in the establishment of the spatial pattern of gene expression. During midgut development in Drosophila, secreted molecules of Decapentaplegic (Dpp) and Wingless (Wg) establish unique transcriptional regulation within target cells to specify the resultant c...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.12.17.2724

    authors： Nakagoshi H,Hoshi M,Nabeshima Y,Matsuzaki F

    更新日期：1998-09-01 00:00:00

  • Nuclear receptor-like transcription factors in fungi.

    abstract：:Members of the metazoan nuclear receptor superfamily regulate gene expression programs in response to binding of cognate lipophilic ligands. Evolutionary studies using bioinformatics tools have concluded that lower eukaryotes, such as fungi, lack nuclear receptor homologs. Here we review recent discoveries suggesting ...

    journal_title：Genes & development

    pub_type： 杂志文章,评审

    doi：10.1101/gad.1743009

    authors： Näär AM,Thakur JK

    更新日期：2009-02-15 00:00:00

  • Centriole distal appendages promote membrane docking, leading to cilia initiation.

    abstract：:The distal appendages (DAPs) of centrioles have been proposed to anchor cilia to the plasma membrane, but their molecular composition, assembly, and exact function in ciliogenesis remain poorly understood. Using quantitative centrosome proteomics and superresolution microscopy, we identified five DAP components, inclu...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.207043.112

    authors： Tanos BE,Yang HJ,Soni R,Wang WJ,Macaluso FP,Asara JM,Tsou MF

    更新日期：2013-01-15 00:00:00