Functional domains of the yeast transcription/replication factor MCM1.

abstract：:Known prions (infectious proteins) are self-propagating amyloids or conformationally altered proteins, but in theory an enzyme necessary for its own activation could also be a prion (or a gene composed of protein). We show that yeast protease B is such a prion, called [beta].[beta] is infectious, reversibly curable, a...

journal_title：Genes & development

authors： Roberts BT,Wickner RB

更新日期：2003-09-01 00:00:00

abstract：:The embryonic lethal abnormal visual system (ELAV) is a gene-specific regulator of alternative pre-mRNA processing in neurons of Drosophila. Here we define a functional in vivo binding site for ELAV in neurons through the development of a reporter gene system in transgenic animals in combination with in vitro binding ...

journal_title：Genes & development

authors： Soller M,White K

更新日期：2003-10-15 00:00:00

abstract：:Interactions required for inhibition of poly(A) polymerase (PAP) by the U1 snRNP-specific U1A protein, a reaction whose function is to autoregulate U1A protein production, are examined. PAP inhibition requires a substrate RNA to which at least two molecules of U1A protein can bind tightly, but we demonstrate that the ...

journal_title：Genes & development

authors： Gunderson SI,Vagner S,Polycarpou-Schwarz M,Mattaj IW

更新日期：1997-03-15 00:00:00

abstract：:We identify the "M region" of the muscle-specific Xenopus cardiac actin gene promoter from -282 to -348 as necessary for the embryonic expression of a cardiac actin-beta-globin reporter gene injected into fertilized eggs. Four DNA-binding activities in embryo extracts, embryonic M-region factors 1-4 (EMF1-4), are desc...

journal_title：Genes & development

authors： Taylor MV,Gurdon JB,Hopwood ND,Towers N,Mohun TJ

更新日期：1991-07-01 00:00:00

abstract：:Discrete sequence elements known as exonic splicing enhancers (ESEs) have been shown to influence both the efficiency of splicing and the profile of mature mRNAs in multicellular eukaryotes. While the existence of ESEs has not been demonstrated previously in unicellular eukaryotes, the factors known to recognize these...

journal_title：Genes & development

authors： Webb CJ,Romfo CM,van Heeckeren WJ,Wise JA

更新日期：2005-01-15 00:00:00

abstract：:Cyclin B3 has been conserved during higher eukaryote evolution as evidenced by its identification in chicken, nematodes, and insects. We demonstrate that Cyclin B3 is present in addition to Cyclins A and B in mitotically proliferating cells and not detectable in endoreduplicating tissues of Drosophila embryos. Cyclin ...

journal_title：Genes & development

authors： Jacobs HW,Knoblich JA,Lehner CF

更新日期：1998-12-01 00:00:00

abstract：:RNA interference (RNAi) is a flexible gene silencing mechanism that responds to double-stranded RNA by suppressing homologous genes. Here, we report the characterization of RNAi effector complexes (RISCs) that contain small interfering RNAs and microRNAs (miRNAs). We identify two putative RNA-binding proteins, the Dro...

journal_title：Genes & development

authors： Caudy AA,Myers M,Hannon GJ,Hammond SM

更新日期：2002-10-01 00:00:00

abstract：:Human lymphoblastoid cells derived from different healthy individuals display considerable variation in their transcription profiles. Here we show that such variation in gene expression underlies interindividual susceptibility to DNA damaging agents. The results demonstrate the massive differences in sensitivity acros...

journal_title：Genes & development

authors： Fry RC,Svensson JP,Valiathan C,Wang E,Hogan BJ,Bhattacharya S,Bugni JM,Whittaker CA,Samson LD

更新日期：2008-10-01 00:00:00

abstract：:The canoemisty1 (cnomis1) mutation was isolated by virtue of its severe rough eye phenotype from approximately 500 fly lines, each harboring a single autosomal insertion of a P element (Bm delta w). Excision of the P element generated a lethal, null allele, cnomis10, together with many revertants with normal eye morph...

journal_title：Genes & development

authors： Miyamoto H,Nihonmatsu I,Kondo S,Ueda R,Togashi S,Hirata K,Ikegami Y,Yamamoto D

更新日期：1995-03-01 00:00:00

abstract：:PIWI clade Argonaute proteins silence transposon expression in animal gonads. Their target specificity is defined by bound ∼23- to 30-nucleotide (nt) PIWI-interacting RNAs (piRNAs) that are processed from single-stranded precursor transcripts via two distinct pathways. Primary piRNAs are defined by the endonuclease Zu...

journal_title：Genes & development

authors： Senti KA,Jurczak D,Sachidanandam R,Brennecke J

更新日期：2015-08-15 00:00:00

abstract：:Cell cycle transitions are often accompanied by the degradation of regulatory molecules. Targeting proteins to the proteasome for degradation is accomplished by the covalent addition of ubiquitin chains. The specificity of this pathway is largely dictated by a set of enzymes called ubiquitin ligases (or E3s). The anap...

journal_title：Genes & development

authors： Thornton BR,Toczyski DP

更新日期：2006-11-15 00:00:00

abstract：:A relevant, yet little recognized feature of antisense regulation is the possibility of switching roles between regulatory and regulated RNAs. Here we show that induction of a Salmonella gene relies on the conversion of a small RNA from effector to regulatory target. The chiP gene (formerly ybfM), identified and chara...

journal_title：Genes & development

authors： Figueroa-Bossi N,Valentini M,Malleret L,Fiorini F,Bossi L

更新日期：2009-09-01 00:00:00

abstract：:Abcission, the natural shedding of leaves, flowers and fruits, is a fundamental component of plant development. Abscission is a highly regulated process that occurs at distinct zones of cells that undergo enlargement and subsequent separation. Although some components of abscission, including accumulation of the hormo...

journal_title：Genes & development

authors： Jinn TL,Stone JM,Walker JC

更新日期：2000-01-01 00:00:00

abstract：:The dihydrofolate reductase (DHFR) and 2BE2121 genes in the Chinese hamster are convergently transcribed in late G1 and ea ly S phase, and bracket an early-firing origin of replication that consists of a 55-kb zone of potential initiation sites. To test whether transcription through the DHFR gene is required to activa...

journal_title：Genes & development

authors： Saha S,Shan Y,Mesner LD,Hamlin JL

更新日期：2004-02-15 00:00:00

abstract：:The distal enhancer of the T-cell receptor (TCR) alpha chain gene has become a paradigm for studies of the assembly and activity of architectural enhancer complexes. Here we have reconstituted regulated TCR alpha enhancer activity in vitro on chromatin templates using purified T-cell transcription factors (LEF-1, AML1...

journal_title：Genes & development

authors： Mayall TP,Sheridan PL,Montminy MR,Jones KA

更新日期：1997-04-01 00:00:00

abstract：:Morphogen gradients of secreted molecules play critical roles in the establishment of the spatial pattern of gene expression. During midgut development in Drosophila, secreted molecules of Decapentaplegic (Dpp) and Wingless (Wg) establish unique transcriptional regulation within target cells to specify the resultant c...

journal_title：Genes & development

authors： Nakagoshi H,Hoshi M,Nabeshima Y,Matsuzaki F

更新日期：1998-09-01 00:00:00

abstract：:RNAi has revolutionized reverse genetics; however, RNAi is not necessarily ubiquitous or constitutive. Lund and colleagues (pp. 1121-1131) show that microRNA (miRNA) effector Argonautes (Agos) are limiting and easily saturated during early Xenopus embryogenesis. Moreover, this stage is devoid of slicing capacity. Supp...

journal_title：Genes & development

authors： Flynt AS,Lai EC

更新日期：2011-06-01 00:00:00

abstract：:Signals from TGF-beta superfamily receptors are transduced to the nucleus by Smad proteins, which transcriptionally activate target genes. In Caenorhabditis elegans, defects in a TGF-beta-related pathway cause a reversible developmental arrest and metabolic shift at the dauer larval stage. Null mutations in daf-3 supp...

journal_title：Genes & development

authors： Patterson GI,Koweek A,Wong A,Liu Y,Ruvkun G

更新日期：1997-10-15 00:00:00

abstract：:We report a systematic mutational analysis of the helix-turn-helix motif (HTH) of the fushi tarazu (ftz) homeo domain (HD) of Drosophila. We started out by testing the function of chimeric ftz proteins containing either a part of the Sex combs reduced (Scr) or the muscle segment homeobox (msh) HDs. By complementation ...

journal_title：Genes & development

authors： Furukubo-Tokunaga K,Müller M,Affolter M,Pick L,Kloter U,Gehring WJ

更新日期：1992-06-01 00:00:00

abstract：:The current model for endocrine cell specification in the pancreas invokes high-level production of the transcription factor Neurogenin 3 (Neurog3) in Sox9(+) bipotent epithelial cells as the trigger for endocrine commitment, cell cycle exit, and rapid delamination toward proto-islet clusters. This model posits a tran...

journal_title：Genes & development

authors： Bechard ME,Bankaitis ED,Hipkens SB,Ustione A,Piston DW,Yang YP,Magnuson MA,Wright CV

更新日期：2016-08-15 00:00:00

abstract：:Over a period of many months, Xenopus oocytes stockpile large quantities of tRNA for use during the first few hours of embryogenesis. To test the idea that these tRNAs are transcribed from one set of genes and that another set is used by somatic cells, we used synthetic oligonucleotides to analyze the sequence and ste...

journal_title：Genes & development

authors： Stutz F,Gouilloud E,Clarkson SG

更新日期：1989-08-01 00:00:00

abstract：:AMP-activated protein kinase (AMPK) is a sensor of energy status that maintains cellular energy homeostasis. It arose very early during eukaryotic evolution, and its ancestral role may have been in the response to starvation. Recent work shows that the kinase is activated by increases not only in AMP, but also in ADP....

journal_title：Genes & development

authors： Hardie DG

更新日期：2011-09-15 00:00:00

abstract：:In petunia flowers, the loci an1, an2, and an11 control the pigmentation of the flower by stimulating the transcription of anthocyanin biosynthetic genes. The an1 and an2 locus were recently cloned and encode a basic helix-loop-helix (bHLH) and MYB-domain transcriptional activator, respectively. Here, we report the is...

journal_title：Genes & development

authors： de Vetten N,Quattrocchio F,Mol J,Koes R

更新日期：1997-06-01 00:00:00

abstract：:The basic helix-loop-helix (bHLH) gene Hes7, a putative Notch effector, encodes a transcriptional repressor. Here, we found that Hes7 expression oscillates in 2-h cycles in the presomitic mesoderm (PSM). In Hes7-null mice, somites are not properly segmented and their anterior-posterior polarity is disrupted. As a resu...

journal_title：Genes & development

authors： Bessho Y,Sakata R,Komatsu S,Shiota K,Yamada S,Kageyama R

更新日期：2001-10-15 00:00:00

abstract：:H4K20 methylation is a broad chromatin modification that has been linked with diverse epigenetic functions. Several enzymes target H4K20 methylation, consistent with distinct mono-, di-, and trimethylation states controlling different biological outputs. To analyze the roles of H4K20 methylation states, we generated c...

journal_title：Genes & development

authors： Schotta G,Sengupta R,Kubicek S,Malin S,Kauer M,Callén E,Celeste A,Pagani M,Opravil S,De La Rosa-Velazquez IA,Espejo A,Bedford MT,Nussenzweig A,Busslinger M,Jenuwein T

更新日期：2008-08-01 00:00:00

abstract：:Programmed cell death occurs in a highly reproducible manner during Caenorhabditis elegans development. We demonstrate that, during embryogenesis, miR-35 and miR-58 bantam family microRNAs (miRNAs) cooperate to prevent the precocious death of mothers of cells programmed to die by repressing the gene egl-1, which encod...

journal_title：Genes & development

authors： Sherrard R,Luehr S,Holzkamp H,McJunkin K,Memar N,Conradt B

更新日期：2017-01-15 00:00:00

abstract：:Although Apc is well characterized as a tumor-suppressor gene in the intestine, the precise mechanism of this suppression remains to be defined. Using a novel inducible Ahcre transgenic line in conjunction with a loxP-flanked Apc allele we, show that loss of Apc acutely activates Wnt signaling through the nuclear accu...

journal_title：Genes & development

authors： Sansom OJ,Reed KR,Hayes AJ,Ireland H,Brinkmann H,Newton IP,Batlle E,Simon-Assmann P,Clevers H,Nathke IS,Clarke AR,Winton DJ

更新日期：2004-06-15 00:00:00

abstract：:Adenomatous polyposis coli (APC) regulates the activity of β-catenin, an integral component of Wnt signaling. However, the selective role of the APC-β-catenin pathway in cerebral cortical development is unknown. Here we genetically dissected the relative contributions of APC-regulated β-catenin signaling in cortical p...

journal_title：Genes & development

authors： Nakagawa N,Li J,Yabuno-Nakagawa K,Eom TY,Cowles M,Mapp T,Taylor R,Anton ES

更新日期：2017-08-15 00:00:00

abstract：:Dosage compensation is a process required to balance the expression of X-linked genes between males and females. In Drosophila this is achieved by targeting the dosage compensation complex or the male-specific lethal (MSL) complex to the male X chromosome. In order to study the mechanism of targeting, we have studied ...

journal_title：Genes & development

authors： Kind J,Akhtar A

更新日期：2007-08-15 00:00:00

abstract：:The ubiquitin-specific protease (USP) family is the largest group of cysteine proteases. Cancer genomic analysis identified frequent amplification of USP21 (22%) in human pancreatic ductal adenocarcinoma (PDAC). USP21 overexpression correlates with human PDAC progression, and enforced expression of USP21 accelerates m...

journal_title：Genes & development

authors： Hou P,Ma X,Zhang Q,Wu CJ,Liao W,Li J,Wang H,Zhao J,Zhou X,Guan C,Ackroyd J,Jiang S,Zhang J,Spring DJ,Wang YA,DePinho RA

更新日期：2019-10-01 00:00:00