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RNAi in Xenopus: look before you leap.

Abstract：

:RNAi has revolutionized reverse genetics; however, RNAi is not necessarily ubiquitous or constitutive. Lund and colleagues (pp. 1121-1131) show that microRNA (miRNA) effector Argonautes (Agos) are limiting and easily saturated during early Xenopus embryogenesis. Moreover, this stage is devoid of slicing capacity. Supplementation of Ago proteins rescued endogenous miRNA activity in the presence of exogenous siRNAs, and, excitingly, ectopic Ago2 could now support RNAi in Xenopus. These observations may potentially facilitate RNAi in other recalcitrant model organisms.

journal_name

Genes Dev

journal_title

Genes & development

authors

Flynt AS,Lai EC

doi

10.1101/gad.2062911

subject

Has Abstract

pub_date

2011-06-01 00:00:00

pages

1105-8

issue

11

eissn

0890-9369

issn

1549-5477

pii

25/11/1105

journal_volume

25

pub_type

评论,杂志文章

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