Abstract:
:Cellular morphology is an essential determinant of cellular function in all kingdoms of life, yet little is known about how cell shape is controlled. Here we describe a molecular program that controls the early morphology of neurons through a metazoan-specific zinc finger protein, Unkempt. Depletion of Unkempt in mouse embryos disrupts the shape of migrating neurons, while ectopic expression confers neuronal-like morphology to cells of different nonneuronal lineages. We found that Unkempt is a sequence-specific RNA-binding protein and identified its precise binding sites within coding regions of mRNAs linked to protein metabolism and trafficking. RNA binding is required for Unkempt-induced remodeling of cellular shape and is directly coupled to a reduced production of the encoded proteins. These findings link post-transcriptional regulation of gene expression with cellular shape and have general implications for the development and disease of multicellular organisms.
journal_name
Genes Devjournal_title
Genes & developmentauthors
Murn J,Zarnack K,Yang YJ,Durak O,Murphy EA,Cheloufi S,Gonzalez DM,Teplova M,Curk T,Zuber J,Patel DJ,Ule J,Luscombe NM,Tsai LH,Walsh CA,Shi Ydoi
10.1101/gad.258483.115subject
Has Abstractpub_date
2015-03-01 00:00:00pages
501-12issue
5eissn
0890-9369issn
1549-5477pii
29/5/501journal_volume
29pub_type
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journal_title:Genes & development
pub_type: 杂志文章
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pub_type: 评论
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