In vivo analysis of the helix-turn-helix motif of the fushi tarazu homeo domain of Drosophila melanogaster.

Abstract:

:We report a systematic mutational analysis of the helix-turn-helix motif (HTH) of the fushi tarazu (ftz) homeo domain (HD) of Drosophila. We started out by testing the function of chimeric ftz proteins containing either a part of the Sex combs reduced (Scr) or the muscle segment homeobox (msh) HDs. By complementation tests in transgenic flies, cotransfection assays in cultured Drosophila cells and in vitro DNA-binding assays, we have found that the ftz activity is retained in the ftz-Scr chimera but is lost in the ftz-msh chimera, which is defective in binding to an Antennapedia (Antp)-class target site. Further studies with a series of back-mutants of the ftz-msh chimera have revealed that a set of class-specific DNA backbone-contacting residues in the HTH, particularly Arg-28 and Arg-43, are required for efficient target site recognition and, hence, full ftz activity both in vitro and in vivo.

journal_name

Genes Dev

journal_title

Genes & development

authors

Furukubo-Tokunaga K,Müller M,Affolter M,Pick L,Kloter U,Gehring WJ

doi

10.1101/gad.6.6.1082

subject

Has Abstract

pub_date

1992-06-01 00:00:00

pages

1082-96

issue

6

eissn

0890-9369

issn

1549-5477

journal_volume

6

pub_type

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