Phase separation of Polycomb-repressive complex 1 is governed by a charged disordered region of CBX2.

abstract：:We used Sarkosyl to analyze steps along the pathway of transcription initiation by RNA polymerase III. Sarkosyl (0.015%) inhibited transcription when present prior to incubation of RNA polymerase III, TFIIIB, and TFIIIC with the VAI gene, whereas it had no detectable effect on initiation or reinitiation of transcripti...

journal_title：Genes & development

authors： Kovelman R,Roeder RG

更新日期：1990-04-01 00:00:00

abstract：:In Escherichia coli, the major nucleoid protein H-NS limits transcription by acting as a repressor or transcriptional silencer, presumably by its ability to close the looped chromosome domains in the nucleoid through DNA-protein-DNA bridging. Here, we demonstrate the direct involvement of H-NS as a positive factor sti...

journal_title：Genes & development

authors： Park HS,Ostberg Y,Johansson J,Wagner EG,Uhlin BE

更新日期：2010-07-01 00:00:00

abstract：:Eukaryotic RNAs with premature termination codons (PTCs) are eliminated by nonsense-mediated decay (NMD). While human nonsense RNA degradation can be initiated either by an endonucleolytic cleavage event near the PTC or through decapping, the individual contribution of these activities on endogenous substrates has rem...

journal_title：Genes & development

authors： Lykke-Andersen S,Chen Y,Ardal BR,Lilje B,Waage J,Sandelin A,Jensen TH

更新日期：2014-11-15 00:00:00

abstract：:Reactive oxygen species (ROS) participate in numerous cell responses, including proliferation, DNA damage, and cell death. Based on these disparate activities, both promotion and inhibition of ROS have been proposed for cancer therapy. However, how the ROS response is determined is not clear. We examined the activitie...

journal_title：Genes & development

authors： Cheung EC,Lee P,Ceteci F,Nixon C,Blyth K,Sansom OJ,Vousden KH

更新日期：2016-01-01 00:00:00

abstract：:Establishment of the steroid-producing Leydig cell lineage is an event downstream of Sry that is critical for masculinization of mammalian embryos. Neither the origin of fetal Leydig cell precursors nor the signaling pathway that specifies the Leydig cell lineage is known. Based on the sex-specific expression patterns...

journal_title：Genes & development

authors： Yao HH,Whoriskey W,Capel B

更新日期：2002-06-01 00:00:00

abstract：:Eukaryotic chromosomes reach their stable rod-shaped appearance in mitosis in a reaction dependent on the evolutionarily conserved condensin complex. Little is known about how and where condensin associates with chromosomes. Here, we analyze condensin binding to budding yeast chromosomes using high-resolution oligonuc...

journal_title：Genes & development

authors： D'Ambrosio C,Schmidt CK,Katou Y,Kelly G,Itoh T,Shirahige K,Uhlmann F

更新日期：2008-08-15 00:00:00

abstract：:The Hedgehog (Hh) family of secreted proteins controls many aspects of animal development. In Drosophila, Hh transduces its signal via Cubitus interruptus (Ci), a transcription factor present in two forms: a full-length activator and a carboxy-terminally truncated repressor that is derived from the full-length form by...

journal_title：Genes & development

authors： Wang G,Wang B,Jiang J

更新日期：1999-11-01 00:00:00

abstract：:Known prions (infectious proteins) are self-propagating amyloids or conformationally altered proteins, but in theory an enzyme necessary for its own activation could also be a prion (or a gene composed of protein). We show that yeast protease B is such a prion, called [beta].[beta] is infectious, reversibly curable, a...

journal_title：Genes & development

authors： Roberts BT,Wickner RB

更新日期：2003-09-01 00:00:00

abstract：:The lysosomal storage disorder galactosialidosis results from a primary deficiency of the protective protein/cathepsin A (PPCA), which in turn affects the activities of beta-galactosidase and neuraminidase. Mice homozygous for a null mutation at the PPCA locus present with signs of the disease shortly after birth and ...

journal_title：Genes & development

authors： Zhou XY,Morreau H,Rottier R,Davis D,Bonten E,Gillemans N,Wenger D,Grosveld FG,Doherty P,Suzuki K,Grosveld GC,d'Azzo A

更新日期：1995-11-01 00:00:00

abstract：:Hypocretin (orexin; Hcrt)-containing neurons of the hypothalamus are essential for the normal regulation of sleep and wake behaviors and have been implicated in feeding, anxiety, depression, and reward. The absence of these neurons causes narcolepsy in humans and model organisms. However, little is known about the mol...

journal_title：Genes & development

authors： Dalal J,Roh JH,Maloney SE,Akuffo A,Shah S,Yuan H,Wamsley B,Jones WB,de Guzman Strong C,Gray PA,Holtzman DM,Heintz N,Dougherty JD

更新日期：2013-03-01 00:00:00

abstract：:Ferredoxin reductase (FDXR), a target of p53, modulates p53-dependent apoptosis and is necessary for steroidogenesis and biogenesis of iron-sulfur clusters. To determine the biological function of FDXR, we generated a Fdxr-deficient mouse model and found that loss of Fdxr led to embryonic lethality potentially due to ...

journal_title：Genes & development

authors： Zhang Y,Qian Y,Zhang J,Yan W,Jung YS,Chen M,Huang E,Lloyd K,Duan Y,Wang J,Liu G,Chen X

更新日期：2017-06-15 00:00:00

abstract：:Caenorhabditis elegans let-7, a founding member of the microRNA family, is predicted to bind to six sites in the 3'UTR of the mRNA of its target gene, lin-41, to down-regulate LIN-41. Here, we demonstrate that wild-type let-7 microRNA binds in vitro to RNA from the lin-41 3'UTR. This interaction is dependent on two co...

journal_title：Genes & development

authors： Vella MC,Choi EY,Lin SY,Reinert K,Slack FJ

更新日期：2004-01-15 00:00:00

abstract：:GRF2, an abundant yeast protein of Mr approximately 127,000, binds to the GAL upstream activating sequence (UASG) and creates a nucleosome-free region of approximately 230 bp. Purified GRF2 binds to sequences found in many other UASs, in the 35S rRNA enhancer, at centromeres, and at telomeres. Although GRF2 stimulates...

journal_title：Genes & development

authors： Chasman DI,Lue NF,Buchman AR,LaPointe JW,Lorch Y,Kornberg RD

更新日期：1990-04-01 00:00:00

abstract：:Transcriptional activation of the human U1 snRNA genes is dependent on a noncanonical octamer element contained within an upstream enhancer. The U1 octamer only weakly recruits the Oct-1 POU domain, although recruitment is stimulated by a peptide containing the Oct-1-binding domain of SNAP190. Structural analysis of t...

journal_title：Genes & development

authors： Hovde S,Hinkley CS,Strong K,Brooks A,Gu L,Henry RW,Geiger J

更新日期：2002-11-01 00:00:00

abstract：:Apoptosis is an important mechanism to eliminate potentially tumorigenic cells. The tumor suppressor p53 plays a pivotal role in this process. Many tumors harbor mutant p53, but others evade its tumor-suppressive effects by altering the expression of proteins that regulate the p53 pathway. ASPP1 (apoptosis-stimulating...

journal_title：Genes & development

authors： Aylon Y,Ofir-Rosenfeld Y,Yabuta N,Lapi E,Nojima H,Lu X,Oren M

更新日期：2010-11-01 00:00:00

abstract：:The RecQ protein family is a highly conserved group of DNA helicases that play roles in maintaining genomic stability. In this study, we present biochemical and genetic evidence that Escherichia coli RecQ processes stalled replication forks and participates in SOS signaling. Cells that carry dnaE486, a mutation in the...

journal_title：Genes & development

authors： Hishida T,Han YW,Shibata T,Kubota Y,Ishino Y,Iwasaki H,Shinagawa H

更新日期：2004-08-01 00:00:00

abstract：:How RNA-binding proteins recognize specific sets of target mRNAs remains poorly understood because current approaches depend primarily on sequence information. In this study, we demonstrate that specific recognition of messenger RNAs (mRNAs) by RNA-binding proteins requires the correct spatial positioning of these seq...

journal_title：Genes & development

authors： Patel VL,Mitra S,Harris R,Buxbaum AR,Lionnet T,Brenowitz M,Girvin M,Levy M,Almo SC,Singer RH,Chao JA

更新日期：2012-01-01 00:00:00

abstract：:In recent years several new mouse models for lung cancer have been described. These include models for both non-small-cell lung cancer (NSCLC) and small-cell lung cancer (SCLC). Tumorigenesis in these conditional mouse tumor models can be initiated in adult mice through Cre-recombinase-induced activation of oncogenic ...

journal_title：Genes & development

authors： Meuwissen R,Berns A

更新日期：2005-03-15 00:00:00

abstract：:Calcineurin, a conserved Ca(2+)/calmodulin-regulated protein phosphatase, plays a crucial role in Ca(2+) signaling in a wide variety of cell types. In Saccharomyces cerevisiae, calcineurin positively regulates transcription in response to stress by dephosphorylating the transcription factor Crz1p/Tcn1p. Dephosphorylat...

journal_title：Genes & development

authors： Boustany LM,Cyert MS

更新日期：2002-03-01 00:00:00

abstract：:A relevant, yet little recognized feature of antisense regulation is the possibility of switching roles between regulatory and regulated RNAs. Here we show that induction of a Salmonella gene relies on the conversion of a small RNA from effector to regulatory target. The chiP gene (formerly ybfM), identified and chara...

journal_title：Genes & development

authors： Figueroa-Bossi N,Valentini M,Malleret L,Fiorini F,Bossi L

更新日期：2009-09-01 00:00:00

abstract：:Cap-dependent translation is mediated by eIF4F, a protein complex composed of three subunits as follows: eIF4E, which recognizes the mRNA 5' cap structure; eIF4A, an RNA-helicase; and eIF4G, a scaffolding protein that binds eIF4E, eIF4A, and the eIF4E-kinase Mnk1 simultaneously. eIF4E is hypophosphorylated and cap-dep...

journal_title：Genes & development

authors： Pyronnet S,Dostie J,Sonenberg N

更新日期：2001-08-15 00:00:00

abstract：:Signal transduction through the FGF receptor is essential for the specification of the vertebrate body plan. Blocking the FGF pathway in early Xenopus embryos inhibits mesoderm induction and results in truncation of the anterior-posterior axis. The Drosophila gene sprouty encodes an antagonist of FGF signaling, which ...

journal_title：Genes & development

authors： Nutt SL,Dingwell KS,Holt CE,Amaya E

更新日期：2001-05-01 00:00:00

abstract：:Pcf11 is one of numerous proteins involved in pre-mRNA 3'-end processing and transcription termination. Using elongation complexes (ECs) formed from purified yeast RNA polymerase II (Pol II), we show that a 140-amino acid polypeptide from yeast Pcf11 is capable of dismantling the EC in vitro. This action depends on th...

journal_title：Genes & development

authors： Zhang Z,Fu J,Gilmour DS

更新日期：2005-07-01 00:00:00

abstract：:The Bloom's syndrome gene (BLM) plays a pivotal role in the maintenance of genomic stability in somatic cells. It encodes a DNA helicase (BLM) of the RecQ family, but the exact function of BLM remains elusive. To study this question, we have cloned the BLM homolog of the frog Xenopus laevis (xBLM) and have raised anti...

journal_title：Genes & development

authors： Liao S,Graham J,Yan H

更新日期：2000-10-15 00:00:00

abstract：:The embryonic ectoderm in Drosophila displays a highly organized arrangement of specific structures along the dorsal-ventral axis. To establish this characteristic design, cells must receive instructive cues regarding their position. We present evidence that during stages 8-9 of embryonic development, the Drosophila E...

journal_title：Genes & development

authors： Raz E,Shilo BZ

更新日期：1993-10-01 00:00:00

abstract：:We have developed a simple, effective genetic screen for mutant alleles of eukaryotic DNA topoisomerase I that manifest severely depressed or complete loss of enzymatic function. The screen is based on the extreme toxicity of vaccinia topoisomerase expression in the Escherichia coli lysogen strain BL21(DE3) and is not...

journal_title：Genes & development

authors： Morham SG,Shuman S

更新日期：1990-04-01 00:00:00

abstract：:Cohesin is a chromosome-associated multisubunit protein complex that is highly conserved in eukaryotes and has close homologs in bacteria. Cohesin mediates cohesion between replicated sister chromatids and is therefore essential for chromosome segregation in dividing cells. Cohesin is also required for efficient repai...

journal_title：Genes & development

authors： Peters JM,Tedeschi A,Schmitz J

更新日期：2008-11-15 00:00:00

abstract：:Ecsit is a cytosolic adaptor protein essential for inflammatory response and embryonic development via the Toll-like and BMP (bone morphogenetic protein) signal transduction pathways, respectively. Here, we demonstrate a mitochondrial function for Ecsit (an evolutionary conserved signaling intermediate in Toll pathway...

journal_title：Genes & development

authors： Vogel RO,Janssen RJ,van den Brand MA,Dieteren CE,Verkaart S,Koopman WJ,Willems PH,Pluk W,van den Heuvel LP,Smeitink JA,Nijtmans LG

更新日期：2007-03-01 00:00:00

abstract：:Regulated cell expansion is an important determinant of organ shape in higher plants. The sabre mutation results in abnormal cell expansion in Arabidopsis. There is a shift in the orientation of expansion evident primarily in root cortex cells. The SABRE gene has been cloned and found to encode a novel protein. Reduct...

journal_title：Genes & development

authors： Aeschbacher RA,Hauser MT,Feldmann KA,Benfey PN

更新日期：1995-02-01 00:00:00

abstract：:U4 and U6 small nuclear RNAs are associated by an extensive base-pairing interaction that must be disrupted and reformed with each round of splicing. U4 mutations within the U4/U6 interaction domain destabilize the complex in vitro and cause a cold-sensitive phenotype in vivo. Restabilization of the U4/U6 helix by dom...

journal_title：Genes & development

authors： Shannon KW,Guthrie C

更新日期：1991-05-01 00:00:00