Abstract:
:The SNF1 protein kinase is broadly conserved in eukaryotes and has been implicated in responses to environmental and nutritional stress. In yeast, the SNF1 kinase has a central role in the response to glucose starvation. SNF1 is associated with its activating subunit, SNF4, and other proteins in complexes. Using the two-hybrid system, we show that interaction between SNF1 and SNF4 is strongly regulated by the glucose signal. Moreover, this interaction is appropriately affected by mutations in regulators, including protein phosphatase 1. We show that SNF4 binds to the SNF1 regulatory domain in low glucose, whereas in high glucose the regulatory domain binds to the kinase domain of SNF1 itself. Genetic analysis further suggests that the SNF1 regulatory domain autoinhibits the kinase activity and that in low glucose SNF4 antagonizes this inhibition. Finally, these interactions have been conserved from yeast to plants, indicating that homologs of the SNF1 kinase complex respond to regulatory signals by analogous mechanisms.
journal_name
Genes Devjournal_title
Genes & developmentauthors
Jiang R,Carlson Mdoi
10.1101/gad.10.24.3105subject
Has Abstractpub_date
1996-12-15 00:00:00pages
3105-15issue
24eissn
0890-9369issn
1549-5477journal_volume
10pub_type
杂志文章abstract::The Agrobacterium Ti plasmid (T-DNA) 6b proteins interact with many different host proteins implicated in plant cell proliferation. Here, we show that Arabidopsis plants overexpressing 6b display microRNA (miRNA) deficiency by directly targeting SERRATE and AGO1 via a specific loop fragment (residues 40-55). In additi...
journal_title:Genes & development
pub_type: 杂志文章
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pub_type: 杂志文章,评审
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更新日期:2004-10-15 00:00:00
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pub_type: 杂志文章,评审
doi:10.1101/gad.1437206
更新日期:2006-08-15 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
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更新日期:2002-07-01 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
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更新日期:2012-09-01 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.1617608
更新日期:2008-01-15 00:00:00
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journal_title:Genes & development
pub_type: 评论,杂志文章
doi:10.1101/gad.10.9.1152
更新日期:1996-05-01 00:00:00
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journal_title:Genes & development
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doi:
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journal_title:Genes & development
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journal_title:Genes & development
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更新日期:1989-09-01 00:00:00
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