Abstract:
:The presence of Set2-mediated methylation of H3K36 (K36me) correlates with transcription frequency throughout the yeast genome. K36me targets the Rpd3S complex to deacetylate transcribed regions and suppress cryptic transcription initiation at certain genes. Here, using a genome-wide approach, we report that the Set2-Rpd3S pathway is generally required for controlling acetylation at coding regions. When using acetylation as a functional readout for this pathway, we discovered that longer genes and, surprisingly, genes transcribed at lower frequency exhibit a stronger dependency. Moreover, a systematic screen using high-resolution tiling microarrays allowed us to identify a group of genes that rely on Set2-Rpd3S to suppress spurious transcripts. Interestingly, most of these genes are within the group that depend on the same pathway to maintain a hypoacetylated state at coding regions. These data highlight the importance of using the functional readout of histone codes to define the roles of specific pathways.
journal_name
Genes Devjournal_title
Genes & developmentauthors
Li B,Gogol M,Carey M,Pattenden SG,Seidel C,Workman JLdoi
10.1101/gad.1539307subject
Has Abstractpub_date
2007-06-01 00:00:00pages
1422-30issue
11eissn
0890-9369issn
1549-5477pii
21/11/1422journal_volume
21pub_type
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