Abstract：
:Previous genetic mosaic studies established that expression of the Drosophila bride of sevenless (boss) gene is required in photoreceptor neuron R8 for the development of photoreceptor neuron R7. This led to the proposal that boss encodes or regulates an R7-specific inductive cue. We have identified the boss gene based on small deletions in mutant alleles and sequenced both cDNAs and corresponding genomic regions. One P element and three X-ray-induced boss alleles show different deletions in the gene ranging in size from 2 to 23 bp, each causing frameshifts leading to premature termination of translation. The boss gene encodes a protein of 896 amino acids with a putative amino-terminal signal sequence, a large extracellular region of 498 amino acids, and seven potential transmembrane domains followed by a carboxy-terminal cytoplasmic tail of 115 amino acids. The putative membrane localization of the boss protein is consistent with a model in which direct interaction between the boss and sevenless proteins specifies R7 cell fate. Another model in which the boss protein functions as a receptor is proposed based on its similarity to the G protein-linked family of membrane receptors.
journal_name
Genes Devjournal_title
Genes & developmentauthors
Hart AC,Krämer H,Van Vactor DL Jr,Paidhungat M,Zipursky SLdoi
10.1101/gad.4.11.1835subject
Has Abstractpub_date
1990-11-01 00:00:00pages
1835-47issue
11eissn
0890-9369issn
1549-5477journal_volume
4pub_type
杂志文章abstract：:The PWI motif is a highly conserved domain of unknown function in the SRm160 splicing and 3'-end cleavage-stimulatory factor, as well as in several other known or putative pre-mRNA processing components. We show here that the PWI motif is a new type of RNA/DNA-binding domain that has an equal preference for single- an...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1060403
更新日期：2003-02-15 00:00:00
abstract：:Somatic cell clones often fail at a developmental stage coincident with commencement of differentiation. The transcription factor Oct4 is expressed during cleavage stages and is essential for the differentiation of the blastocyst. Oct4 expression becomes restricted to the inner cell mass and epiblast. After gastrulati...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.966002
更新日期：2002-05-15 00:00:00
abstract：:Many bacterial small regulatory RNAs (sRNAs) pair with mRNA targets, stimulating or inhibiting mRNA stability and/or translation. Regulation of these sRNAs is usually due to tight transcriptional regulation of synthesis.In this issue of Genes & Development and a related paper in Molecular Microbiology, Figueroa-Bossi ...
journal_title：Genes & development
pub_type： 评论,杂志文章
doi：10.1101/gad.1846609
更新日期：2009-09-01 00:00:00
abstract：:The product of the zygotically active decapentaplegic (dpp) gene appears to function as a morphogen that specifies positional information in the dorsal half of the Drosophila embryo. The dorsal-specific transcription of dpp is the key step in establishing a morphogen gradient. We demonstrate here that multiple regions...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.7.4.694
更新日期：1993-04-01 00:00:00
abstract：:Alteration of nucleosomes by ATP-dependent remodeling complexes represents a critical step in the regulation of transcription. The human SWI/SNF (hSWI/SNF) family is composed of complexes that contain either Brg1 or hBrm as the central ATPase; however, these separate complexes have not been compared functionally. Here...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.872801
更新日期：2001-03-01 00:00:00
abstract：:RBP is a cellular protein that functions as a transcriptional repressor in mammalian cells. RBP has elicited great interest lately because of its established roles in regulating gene expression, in Drosophila and mouse development, and as a component of the Notch signal transduction pathway. This report focuses on the...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.12.11.1621
更新日期：1998-06-01 00:00:00
abstract：:We have used the 100-kD HeLa cell octamer-binding protein OBP100 as a model to study flexible DNA sequence recognition by promoter-binding proteins. OBP100 binds to the conserved octamer motif ATGCAAAT found in numerous promoters and additionally to two degenerate octamer motifs (sites I and II) within the SV40 enhanc...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.2.11.1400
更新日期：1988-11-01 00:00:00
abstract：:To find new genes that influence liver lipid mass, we performed a genetic screen for zebrafish mutants with hepatic steatosis, a pathological accumulation of fat. The red moon (rmn) mutant develops hepatic steatosis as maternally deposited yolk is depleted. Conversely, hepatic steatosis is suppressed in rmn mutants by...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.180968.111
更新日期：2012-02-01 00:00:00
abstract：:Studying the early stages of cancer can provide important insight into the molecular basis of the disease. We identified a preneoplastic stage in the patched (ptc) mutant mouse, a model for the brain tumor medulloblastoma. Preneoplastic cells (PNCs) are found in most ptc mutants during early adulthood, but only 15% of...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1759909
更新日期：2009-01-15 00:00:00
abstract：:During Drosophila embryogenesis, ectodermal cells of the lateral epithelium stretch in a coordinated fashion to internalize the amnioserosa cells and close the embryo dorsally. This process, dorsal closure, requires two signaling pathways: the Drosophila Jun-amino-terminal kinase (DJNK) pathway and the Dpp pathway. We...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.11.13.1717
更新日期：1997-07-01 00:00:00
abstract：:In this issue of Genes & Development, Dowen and colleagues (pp. 1515-1528) elegantly unify two previously unconnected aspects of physiology. The investigators provide significant genetic evidence to support a critical link between developmental timing decisions and the regulation of lipid mobilization at the transitio...
journal_title：Genes & development
pub_type： 评论,杂志文章
doi：10.1101/gad.285460.116
更新日期：2016-07-01 00:00:00
abstract：:Faithful duplication of the genome relies on the ability to cope with an imperfect template. We investigated replication of UV-damaged DNA in Escherichia coli and found that ongoing replication stops for at least 15-20 min before resuming. Undamaged origins of replication (oriC) continue to fire at the normal rate and...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.417607
更新日期：2007-03-15 00:00:00
abstract：:Mammalian histone gene transcription is increased approximately fivefold during the transition from the G1 phase to the S phase of the cell cycle. In this study, we present a detailed in vivo analysis of the human histone H2b promoter, which establishes that transcriptional regulation of this gene is mediated by a sub...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.2.1.32
更新日期：1988-01-01 00:00:00
abstract：:Gastrulation in mice is associated with the start of extreme proliferation and differentiation. The potential cost to the embryo of a very rapid proliferation rate is a high production of damaged cells. We demonstrate a novel surveillance mechanism for the elimination of cells damaged by ionizing radiation during mous...
journal_title：Genes & development
pub_type： 杂志文章
doi：
更新日期：2000-08-15 00:00:00
abstract：:Discrete sequence elements known as exonic splicing enhancers (ESEs) have been shown to influence both the efficiency of splicing and the profile of mature mRNAs in multicellular eukaryotes. While the existence of ESEs has not been demonstrated previously in unicellular eukaryotes, the factors known to recognize these...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1265905
更新日期：2005-01-15 00:00:00
abstract：:The mottler of white (mw) locus has been determined to interact with alleles of the white (w) eye color locus which are a subset of the transposable element insertion mutants. The transposable elements belong to six different types, including copia, and are located at several sites within the w gene. Three X-ray-induc...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.3.1.73
更新日期：1989-01-01 00:00:00
abstract：:Plectin, a major linker and scaffolding protein of the cytoskeleton, has been shown to be essential for the mechanical integrity of skin, skeletal muscle, and heart. Studying fibroblast and astroglial cell cultures derived from plectin (-/-) mice, we found that their actin cytoskeleton, including focal adhesion contac...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.12.21.3442
更新日期：1998-11-01 00:00:00
abstract：:Metazoans start embryogenesis with a relatively naïve genome. The transcriptionally inert, late-replicating heterochromatic regions, including the constitutive heterochromatin on repetitive sequences near centromeres and telomeres, need to be re-established during development. To explore the events initiating heteroch...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.272237.115
更新日期：2016-03-01 00:00:00
abstract：:Genetic screening and selection procedures employing a secA-lacZ fusion strain repeatedly have yielded mutations in four genes affecting the protein export pathway of Escherichia coli. These genes are secA, secD, prlA/secY, and secE. We discuss the significance of the failure to find new sec genes after extensive use ...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.3.7.1035
更新日期：1989-07-01 00:00:00
abstract：:It is postulated that the symmetric organization of plant lateral organs is based on two crossed axes, the abaxial-adaxial and the lateral axes. The PRESSED FLOWER (PRS) gene, the expression and function of which are dependent on the lateral axis, is reported in this study. In the prs mutant, growth of the lateral sep...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.931001
更新日期：2001-12-15 00:00:00
abstract：:Upon activation by far-red light, phytochrome A signals are transduced through several pathways to promote photomorphogenesis. The COP1 E3 ligase represses photomorphogenesis in part by targeting transcription activators such as LAF1 and HY5 for destruction. Another positive regulator of photomorphogenesis is HFR1, a ...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1247205
更新日期：2005-03-01 00:00:00
abstract：:The repression of the maternally inherited Igf2 allele has been proposed to depend on a methylation-sensitive chromatin insulator organized by the 11 zinc finger protein CTCF at the H19 imprinting control region (ICR). Here we document that point mutations of the nucleotides in physical contact with CTCF within the en...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.254903
更新日期：2003-03-01 00:00:00
abstract：:Transabdominal (Tab), a dominant mutation in the Bithorax Complex (BX-C) of Drosophila, creates a sexually dimorphic pattern of segmental transformation that has complete penetrance and expressivity. Specific regions within the notum of the second thoracic segment (T2) are transformed into abdominal-like cuticle; thus...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1.2.111
更新日期：1987-04-01 00:00:00
abstract：:Sequence-specific DNA-binding activators, key regulators of gene expression, stimulate transcription in part by targeting the core promoter recognition TFIID complex and aiding in its recruitment to promoter DNA. Although it has been established that activators can interact with multiple components of TFIID, it is unk...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1790709
更新日期：2009-07-01 00:00:00
abstract：:Antigenic stimulation of the B-cell receptor (BCR) is a central event in the immune response. In contrast, antigen bound to IgG negatively regulates signals from the BCR by cross-linking it to the inhibitory receptor FcgammaRIIB. Here we show that upon cross-linking of BCR or BCR with FcgammaRIIB, the rasGAP-associate...
journal_title：Genes & development
pub_type： 杂志文章
doi：
更新日期：2000-01-01 00:00:00
abstract：:Glucose and glutamine serve as the two primary carbon sources in proliferating cells, and uptake of both nutrients is directed by growth factor signaling. Although either glucose or glutamine can potentially support mitochondrial tricarboxylic acid (TCA) cycle integrity and ATP production, we found that glucose depriv...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1985910
更新日期：2010-12-15 00:00:00
abstract：:Cyclin D1 is part of a cell cycle control node consistently deregulated in most human cancers. However, studies with cyclin D1-null mice indicate that it is dispensable for normal mouse development as well as cell growth in culture. Here, we provide evidence that ras-mediated tumorigenesis depends on signaling pathway...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.12.16.2469
更新日期：1998-08-15 00:00:00
abstract：:Stomata are specialized cellular structures in the epidermis of aerial plant organs that control gas exchange (H(2)O release and CO(2) uptake) between leaves and the atmosphere by modulating the aperture of a pore flanked by two guard cells. Stomata are nonrandomly distributed, and their density is controlled by endog...
journal_title：Genes & development
pub_type： 杂志文章
doi：
更新日期：2000-05-01 00:00:00
abstract：:A new gene, SCH9, was isolated from Saccharomyces cerevisiae by its ability to complement a cdc25ts mutation. Sequence analysis indicates that it encodes a 90,000-dalton protein with a carboxy-terminal domain homologous to yeast and mammalian cAMP-dependent protein kinase catalytic subunits. In addition to suppressing...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.2.5.517
更新日期：1988-05-01 00:00:00
abstract：:The Elongin BC complex was identified initially as a positive regulator of RNA polymerase II (Pol II) elongation factor Elongin A and subsequently as a component of the multiprotein von Hippel-Lindau (VHL) tumor suppressor complex, in which it participates in both tumor suppression and negative regulation of hypoxia-i...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.12.24.3872
更新日期：1998-12-15 00:00:00
