Mouse TU tagging: a chemical/genetic intersectional method for purifying cell type-specific nascent RNA.

abstract：:Protein kinases are key regulators of cellular processes. In spite of considerable effort, a full understanding of the pathways they participate in remains elusive. We globally investigated the proteins that interact with the majority of yeast protein kinases using protein microarrays. Eighty-five kinases were purifie...

journal_title：Genes & development

authors： Fasolo J,Sboner A,Sun MG,Yu H,Chen R,Sharon D,Kim PM,Gerstein M,Snyder M

更新日期：2011-04-01 00:00:00

abstract：:The activity of the SCF(skp2) E3 ligase is required for the proteolytic turnover of several proteins involved in cell cycle control and transcriptional regulation. Loss of skp2 in the mouse leads to a complex phenotype including changes in cell size and DNA content as well as severe proliferation defects. Here we show...

journal_title：Genes & development

authors： Kossatz U,Dietrich N,Zender L,Buer J,Manns MP,Malek NP

更新日期：2004-11-01 00:00:00

abstract：:Dosage compensation in Drosophila occurs by a twofold increase in transcription per copy of X-linked genes in males (XY) compared with females (XX). msl-1 is one of four genes that are essential for dosage compensation in males, and MSL-1 protein is associated specifically with the male X chromosome. To explore the ba...

journal_title：Genes & development

authors： Palmer MJ,Richman R,Richter L,Kuroda MI

更新日期：1994-03-15 00:00:00

abstract：:The growth suppression function of the retinoblastoma protein (RB) is mediated by its interaction with a variety of cellular proteins. RB contains at least two protein-binding pockets: the large A/B pocket, which interacts with E2F and the D-type cyclins, and the C pocket, which interacts with the nuclear c-Abl tyrosi...

journal_title：Genes & development

authors： Welch PJ,Wang JY

更新日期：1995-01-01 00:00:00

abstract：:Commitment to mitosis is regulated by a protein kinase complex called MPF. MPF is inhibited by Wee1-related kinases and activated by Cdc25 phosphatase. MPF activation further boosts Cdc25 and represses Wee1. This feedback control probably involves polo kinase. A dominant cut12.s11 mutation in the Schizosaccharomyces p...

journal_title：Genes & development

authors： MacIver FH,Tanaka K,Robertson AM,Hagan IM

更新日期：2003-06-15 00:00:00

abstract：:To investigate the relationship between splicing and polyadenylation during the production of vertebrate mRNAs, we examined the effect of mutation of a poly(A) site on splicing of upstream introns. Mutation of the AAUAAA polyadenylation consensus sequence inhibited in vitro splicing of an upstream intron. The magnitud...

journal_title：Genes & development

authors： Niwa M,Berget SM

更新日期：1991-11-01 00:00:00

abstract：:Direct reprogramming of somatic cells into induced pluripotent stem cells (iPSCs) provides a unique opportunity to derive patient-specific stem cells with potential applications in tissue replacement therapies and without the ethical concerns of human embryonic stem cells (hESCs). However, cellular senescence, which c...

journal_title：Genes & development

authors： Lapasset L,Milhavet O,Prieur A,Besnard E,Babled A,Aït-Hamou N,Leschik J,Pellestor F,Ramirez JM,De Vos J,Lehmann S,Lemaitre JM

更新日期：2011-11-01 00:00:00

abstract：:Pre-mRNA processing is coupled with transcription. It is still unclear if the transcription machinery can also directly affect the cytoplasmic fate of a transcript, such as its intracellular localization. In yeast, the RNA-binding protein She2p binds several mRNAs and targets them for localization at the bud. Here we ...

journal_title：Genes & development

authors： Shen Z,St-Denis A,Chartrand P

更新日期：2010-09-01 00:00:00

abstract：:A conditional block to transcriptional elongation is an important mechanism for regulating c-myc gene expression. This elongation block within the first c-myc exon was defined originally in mammalian cells by nuclear run-on transcription analyses. Subsequent oocyte injection and in vitro transcription analyses suggest...

journal_title：Genes & development

authors： Krumm A,Meulia T,Brunvand M,Groudine M

更新日期：1992-11-01 00:00:00

abstract：:Alternative polyadenylation (APA) is widespread in neuronal development and activity-mediated neural plasticity. However, the underlying molecular mechanisms are largely unknown. We used systematic genetic studies and genome-wide surveys of the transcriptional landscape to identify a context-dependent regulatory pathw...

journal_title：Genes & development

authors： Chen F,Zhou Y,Qi YB,Khivansara V,Li H,Chun SY,Kim JK,Fu XD,Jin Y

更新日期：2015-11-15 00:00:00

abstract：:Alteration of nucleosomes by ATP-dependent remodeling complexes represents a critical step in the regulation of transcription. The human SWI/SNF (hSWI/SNF) family is composed of complexes that contain either Brg1 or hBrm as the central ATPase; however, these separate complexes have not been compared functionally. Here...

journal_title：Genes & development

authors： Sif S,Saurin AJ,Imbalzano AN,Kingston RE

更新日期：2001-03-01 00:00:00

abstract：:Macrophage activation by the proinflammatory cytokine interferon-gamma (IFN-gamma) is a critical component of the host innate response to bacterial pathogenesis. However, the precise nature of the IFN-gamma-induced activation pathway is not known. Here we show using genome-wide expression and chromatin-binding profili...

journal_title：Genes & development

authors： Sonoda J,Laganière J,Mehl IR,Barish GD,Chong LW,Li X,Scheffler IE,Mock DC,Bataille AR,Robert F,Lee CH,Giguère V,Evans RM

更新日期：2007-08-01 00:00:00

abstract：:A Drosophila FGF receptor homolog (DFGF-R2/DFRI) termed Heartless (Htl) is expressed in the embryonic mesoderm. The phenotypes of null mutant embryos demonstrated that Htl is a central player that is required for the development of several mesodermal lineages. No abnormalities in the primary specification of the mesod...

journal_title：Genes & development

authors： Beiman M,Shilo BZ,Volk T

更新日期：1996-12-01 00:00:00

abstract：:To understand the organization of the transcriptional networks that govern cell differentiation, we have investigated the transcriptional circuitry controlling pseudohyphal development in Saccharomyces cerevisiae. The binding targets of Ste12, Tec1, Sok2, Phd1, Mga1, and Flo8 were globally mapped across the yeast geno...

journal_title：Genes & development

authors： Borneman AR,Leigh-Bell JA,Yu H,Bertone P,Gerstein M,Snyder M

更新日期：2006-02-15 00:00:00

abstract：:The N-myc gene encodes a putative transcription factor that is thought to function in the regulation of gene expression during cell differentiation and/or growth. To examine the role of N-myc during development, we have used targeted mutagenesis in embryonic stem cells to produce a mouse line that carries an N-myc nul...

journal_title：Genes & development

authors： Charron J,Malynn BA,Fisher P,Stewart V,Jeannotte L,Goff SP,Robertson EJ,Alt FW

更新日期：1992-12-01 00:00:00

abstract：:Noncoding RNA regulators have been implicated in almost all imaginable cellular processes. Here we review how regulatory small RNAs such as Spot42, SgrS, GlmY, and GlmZ and a cis-encoded ribozyme in glmS mRNA control sugar metabolism. Besides discussing the physiological implications, we show how the study of these mo...

journal_title：Genes & development

authors： Görke B,Vogel J

更新日期：2008-11-01 00:00:00

abstract：:The ATR (ATM and Rad3-related) kinase and its regulatory partner ATRIP (ATR-interacting protein) coordinate checkpoint responses to DNA damage and replication stress. TopBP1 functions as a general activator of ATR. However, the mechanism by which TopBP1 activates ATR is unknown. Here, we show that ATRIP contains a Top...

journal_title：Genes & development

authors： Mordes DA,Glick GG,Zhao R,Cortez D

更新日期：2008-06-01 00:00:00

abstract：:Proliferation of pancreatic islet beta cells is an important mechanism for self-renewal and for adaptive islet expansion. Increased expression of the Ink4a/Arf locus, which encodes the cyclin-dependent kinase inhibitor p16(INK4a) and tumor suppressor p19(Arf), limits beta-cell regeneration in aging mice, but the basis...

journal_title：Genes & development

authors： Chen H,Gu X,Su IH,Bottino R,Contreras JL,Tarakhovsky A,Kim SK

更新日期：2009-04-15 00:00:00

abstract：:An insulin receptor-like signaling pathway regulates Caenorhabditis elegans metabolism, development, and longevity. Inactivation of the insulin receptor homolog DAF-2, the AGE-1 PI3K, or the AKT-1 and AKT-2 kinases causes a developmental arrest at the dauer stage. A null mutation in the daf-16 Fork head transcription ...

journal_title：Genes & development

authors： Paradis S,Ailion M,Toker A,Thomas JH,Ruvkun G

更新日期：1999-06-01 00:00:00

abstract：:Nuclear pore complexes (NPCs) emerged as nuclear transport channels in eukaryotic cells ∼1.5 billion years ago. While the primary role of NPCs is to regulate nucleo-cytoplasmic transport, recent research suggests that certain NPC proteins have additionally acquired the role of affecting gene expression at the nuclear ...

journal_title：Genes & development

authors： Franks TM,Benner C,Narvaiza I,Marchetto MC,Young JM,Malik HS,Gage FH,Hetzer MW

更新日期：2016-05-15 00:00:00

abstract：:Chromosomal translocations are a hallmark of hematopoietic malignancies. The initial molecular events or pathways that lead to translocations in hematopoietic cells are largely unknown, particularly in the stem cell-enriched population postulated to be the initial target for these events. We used in vitro differentiat...

journal_title：Genes & development

authors： Francis R,Richardson C

更新日期：2007-05-01 00:00:00

abstract：:Restricted expression of a mouse Vasa homolog gene (Mvh) expression is first detected in primordial germ cells (PGCs) after colonization of the genital ridges. Subsequently, Mvh is maintained until postmeiotic germ cells are formed. Here, we demonstrate that male mice homozygous for a targeted mutation of Mvh exhibit ...

journal_title：Genes & development

authors： Tanaka SS,Toyooka Y,Akasu R,Katoh-Fukui Y,Nakahara Y,Suzuki R,Yokoyama M,Noce T

更新日期：2000-04-01 00:00:00

abstract：:Chromatin reorganization is essential for transcriptional control by sequence-specific transcription factors. However, the molecular link between transcriptional control and chromatin reconfiguration remains unclear. By colocalization of the nuclear ecdysone receptor (EcR) on the ecdysone-induced puff in the salivary ...

journal_title：Genes & development

authors： Sawatsubashi S,Murata T,Lim J,Fujiki R,Ito S,Suzuki E,Tanabe M,Zhao Y,Kimura S,Fujiyama S,Ueda T,Umetsu D,Ito T,Takeyama K,Kato S

更新日期：2010-01-15 00:00:00

abstract：:The chromo domain was identified as a homologous protein motif between Polycomb (Pc)--a member of the Pc-group genes encoding transcriptional repressors of the homeotic genes--and HP1--a heterochromatin-associated protein encoded by the suppressor of position effect variegation gene Su(var)205. Together with previous ...

journal_title：Genes & development

authors： Messmer S,Franke A,Paro R

更新日期：1992-07-01 00:00:00

abstract：:Histone post-translational modifications regulate chromatin structure and function largely through interactions with effector proteins that often contain multiple histone-binding domains. While significant progress has been made characterizing individual effector domains, the role of paired domains and how they functi...

journal_title：Genes & development

authors： Rothbart SB,Dickson BM,Ong MS,Krajewski K,Houliston S,Kireev DB,Arrowsmith CH,Strahl BD

更新日期：2013-06-01 00:00:00

abstract：:NF-E1, a DNA-binding protein that recognizes the general consensus motif WGATAR, is the first tissue-specific factor to be identified in erythroid cells. Using a probe from the murine GF-1 (NF-E1) cDNA clone, we isolated three homologous chicken cDNAs: One of these corresponds to an mRNA (NF-E1a) that is abundantly an...

journal_title：Genes & development

authors： Yamamoto M,Ko LJ,Leonard MW,Beug H,Orkin SH,Engel JD

更新日期：1990-10-01 00:00:00

abstract：:Transcription of the cell-cycle-regulated human histone genes increases approximately fivefold during S phase. One step toward the elucidation of the biochemical mechanisms that govern cell-cycle-regulated expression of these genes is to purify and characterize the transcription factors that regulate these promoters. ...

journal_title：Genes & development

authors： Dailey L,Roberts SB,Heintz N

更新日期：1988-12-01 00:00:00

abstract：:The cooperation of oncogenes in the transformation of primary rat Schwann cells is a strikingly synergistic process. We have explored the molecular mechanisms involved. Activation of an inducible Raf kinase results in morphologically transformed cells that are arrested in G1 via the induction of p21(CiP1) and subseque...

journal_title：Genes & development

authors： Lloyd AC,Obermüller F,Staddon S,Barth CF,McMahon M,Land H

更新日期：1997-03-01 00:00:00

abstract：:Persistent DNA double-strand breaks and telomeres represent genomic hazards, as they can instigate inappropriate repair reactions. Two recent papers by Oza and colleagues (pp. 912-917) and Schober and colleagues (pp. 928-938) show that both types of DNA ends are sequestered from bulk DNA by Mps3, a SUN domain protein ...

journal_title：Genes & development

authors： Gartenberg MR

更新日期：2009-05-01 00:00:00

abstract：:The distal enhancer of the T-cell receptor (TCR) alpha chain gene has become a paradigm for studies of the assembly and activity of architectural enhancer complexes. Here we have reconstituted regulated TCR alpha enhancer activity in vitro on chromatin templates using purified T-cell transcription factors (LEF-1, AML1...

journal_title：Genes & development

authors： Mayall TP,Sheridan PL,Montminy MR,Jones KA

更新日期：1997-04-01 00:00:00