Abstract：
:The activity of the SCF(skp2) E3 ligase is required for the proteolytic turnover of several proteins involved in cell cycle control and transcriptional regulation. Loss of skp2 in the mouse leads to a complex phenotype including changes in cell size and DNA content as well as severe proliferation defects. Here we show that the loss of a single skp2 substrate, namely, the cyclin kinase inhibitor p27kip1, reverts the phenotype of skp2 knockout hepatocytes to normal. By comparing the kinetics of p27 turnover and cell cycle progression in skp2 knockout and p27T187A knock-in mice, we define a short period in G1 in which p27 is able to block the cell cycle after the exit from quiescence. Loss of p27 turnover during this period prevents mitotic division and instead leads to compensatory cell growth.
journal_name
Genes Devjournal_title
Genes & developmentauthors
Kossatz U,Dietrich N,Zender L,Buer J,Manns MP,Malek NPdoi
10.1101/gad.321004subject
Has Abstractpub_date
2004-11-01 00:00:00pages
2602-7issue
21eissn
0890-9369issn
1549-5477pii
18/21/2602journal_volume
18pub_type
杂志文章abstract：:The transcription factor Ebf1 is an important determinant of early B lymphopoiesis. To gain insight into the functions of Ebf1 at distinct stages of differentiation, we conditionally inactivated Ebf1. We found that Ebf1 is required for the proliferation, survival, and signaling of pro-B cells and peripheral B-cell sub...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.187328.112
更新日期：2012-04-01 00:00:00
abstract：:The Notch signaling pathway is essential for embryonic vascular development in vertebrates. Here we show that mouse embryos heterozygous for a targeted mutation in the gene encoding the DLL4 ligand exhibit haploinsufficient lethality because of defects in vascular remodeling. We also describe vascular defects in embry...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1239204
更新日期：2004-10-15 00:00:00
abstract：:Genomic imprinting is an epigenetic phenomenon leading to parentally biased gene expression. Throughout the years, extensive efforts have been made to characterize the epigenetic marks underlying imprinting in animals and plants. As a result, DNA methylation asymmetries between parental genomes emerged as the primary ...
journal_title：Genes & development
pub_type： 杂志文章,评审
doi：10.1101/gad.332924.119
更新日期：2020-01-01 00:00:00
abstract：:The bcl-6 proto-oncogene encodes a POZ/zinc finger transcriptional repressor expressed in germinal center (GC) B and T cells and required for GC formation and antibody affinity maturation. Deregulation of bcl-6 expression by chromosomal rearrangements and point mutations of the bcl-6 promoter region are implicated in ...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.12.13.1953
更新日期：1998-07-01 00:00:00
abstract：:Studying the early stages of cancer can provide important insight into the molecular basis of the disease. We identified a preneoplastic stage in the patched (ptc) mutant mouse, a model for the brain tumor medulloblastoma. Preneoplastic cells (PNCs) are found in most ptc mutants during early adulthood, but only 15% of...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1759909
更新日期：2009-01-15 00:00:00
abstract：:The physiological functions of many vital tissues and organs continue to mature after birth, but the genetic mechanisms governing this postnatal maturation remain an unsolved mystery. Human pancreatic β cells produce and secrete insulin in response to physiological cues like glucose, and these hallmark functions impro...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.342378.120
更新日期：2021-01-14 00:00:00
abstract：:A new gene, SCH9, was isolated from Saccharomyces cerevisiae by its ability to complement a cdc25ts mutation. Sequence analysis indicates that it encodes a 90,000-dalton protein with a carboxy-terminal domain homologous to yeast and mammalian cAMP-dependent protein kinase catalytic subunits. In addition to suppressing...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.2.5.517
更新日期：1988-05-01 00:00:00
abstract：:Programmed cell death occurs in a highly reproducible manner during Caenorhabditis elegans development. We demonstrate that, during embryogenesis, miR-35 and miR-58 bantam family microRNAs (miRNAs) cooperate to prevent the precocious death of mothers of cells programmed to die by repressing the gene egl-1, which encod...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.288555.116
更新日期：2017-01-15 00:00:00
abstract：:Activated Ras initiates a cascade of sequential phosphorylation events, including the protein kinases Raf, MEK, and MAP kinase. The Let-60 Ras-mediated signal transduction pathway controls vulval induction in Caenorhabditis elegans. Both Lin-45 Raf and Sur-1 MAP kinase have been determined to be essential factors duri...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.9.6.742
更新日期：1995-03-15 00:00:00
abstract：:Classical embryology has provided a clear view of the timing and hormonal cues that govern sexual differentiation. Molecular biology has added important details to this picture. The cloning of SRY, MIS, and INSL3 provide insight into the molecular signals that provide important cues at the cellular level. Continued un...
journal_title：Genes & development
pub_type： 杂志文章,评审
doi：10.1101/gad.843800
更新日期：2000-12-15 00:00:00
abstract：:Genome-wide association studies (GWASs) have identified a genetic variant of moderate effect size at 6p21.1 associated with erythrocyte traits in humans. We show that this variant affects an erythroid-specific enhancer of CCND3. A Ccnd3 knockout mouse phenocopies these erythroid phenotypes, with a dramatic increase in...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.197020.112
更新日期：2012-09-15 00:00:00
abstract：:Infection of metazoan cells with some viruses alters the balance of cellular mRNA export to favor viral RNA export and to retain cellular transcripts in the nucleus. Here, evidence is presented to show that the herpes simplex virus 1 (HSV-1) essential regulatory protein ICP27, which inhibits host cell-splicing, result...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.12.6.868
更新日期：1998-03-15 00:00:00
abstract：:DNA repair associated with DNA replication is important for the conservation of genomic sequence information, whereas reconstitution of chromatin after replication sustains epigenetic information. We have isolated and characterized mutations in the BRU1 gene of Arabidopsis that suggest a novel link between these under...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.295404
更新日期：2004-04-01 00:00:00
abstract：:The amyloid precursor protein (APP) has garnered considerable attention due to its genetic links to Alzheimer's disease. Death receptor 6 (DR6) was recently shown to bind APP via the protein extracellular regions, stimulate axonal pruning, and inhibit synapse formation. Here, we report the crystal structure of the DR6...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.257675.114
更新日期：2015-04-15 00:00:00
abstract：:In budding yeast, a switch between the mutually exclusive pathways of cell cycle progression and conjugation is controlled at Start in late G1 phase. Mating pheromones promote conjugation by arresting cells in G1 phase before Start. Pheromone-induced cell cycle arrest requires a functional FAR1 gene. We have found tha...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.7.5.833
更新日期：1993-05-01 00:00:00
abstract：:The Drosophila somatic sex-determination regulatory pathway has been well studied, but little is known about the target genes that it ultimately controls. In a differential screen for sex-specific transcripts expressed in fly heads, we identified a highly male-enriched transcript encoding Takeout, a protein related to...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1010302
更新日期：2002-11-15 00:00:00
abstract：:Histone methylation plays an important role in regulating gene expression. One such methylation occurs at Lys 79 of histone H3 (H3K79) and is catalyzed by the yeast DOT1 (disruptor of telomeric silencing) and its mammalian homolog, DOT1L. Previous studies have demonstrated that germline disruption of Dot1L in mice res...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.2018511
更新日期：2011-02-01 00:00:00
abstract：:In many species, a dosage compensation complex (DCC) is targeted to X chromosomes of one sex to equalize levels of X-gene products between males (1X) and females (2X). Here we identify cis-acting regulatory elements that target the Caenorhabditis elegans X chromosome for repression by the DCC. The DCC binds to discret...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1751109
更新日期：2009-03-01 00:00:00
abstract：:Changes in the pattern of gene expression play an important role in allowing cancer cells to acquire their hallmark characteristics, while genomic instability enables cells to acquire genetic alterations that promote oncogenesis. Chromatin plays central roles in both transcriptional regulation and the maintenance of g...
journal_title：Genes & development
pub_type： 杂志文章,评审
doi：10.1101/gad.255182.114
更新日期：2015-02-01 00:00:00
abstract：:Caenorhabditis elegans GLD-1, a KH motif containing RNA-binding protein of the GSG/STAR subfamily, controls diverse aspects of germ line development, suggesting that it may have multiple mRNA targets. We used an immunoprecipitation/subtractive hybridization/cloning strategy to identify 15 mRNAs that are putative targe...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.915901
更新日期：2001-09-15 00:00:00
abstract：:Sequence-specific DNA-binding activators, key regulators of gene expression, stimulate transcription in part by targeting the core promoter recognition TFIID complex and aiding in its recruitment to promoter DNA. Although it has been established that activators can interact with multiple components of TFIID, it is unk...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1790709
更新日期：2009-07-01 00:00:00
abstract：:The third-chromosome mutation Killer of prune (K-pn) causes no phenotype by itself, but causes lethality in individuals homozygous for the nonlethal X-chromosome mutation prune (pn). We have recovered 12 gamma-ray-induced revertants of Killer of prune. All of the revertants fail to complement a recessive cell lethal m...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.2.10.1333
更新日期：1988-10-01 00:00:00
abstract：:Lethal yellow (Ay) is a mutation at the mouse agouti (a) locus that is associated with an all-yellow coat color, obesity, diabetes, tumors in heterozygotes, and preimplantation embryonic lethality in homozygotes. Previously, we cloned and characterized the wild-type agouti gene and demonstrated that it expresses a 0.8...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.7.7a.1203
更新日期：1993-07-01 00:00:00
abstract：:Different RNA species are exported from the nucleus by distinct mechanisms. Among the different RNAs, mRNAs and major spliceosomal U snRNAs share several structural similarities, yet they are exported by distinct factors. We previously showed that U1 snRNAs behaved like an mRNA in nuclear export if various approximate...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1216204
更新日期：2004-09-01 00:00:00
abstract：:The Saccharomyces cerevisiae protein kinase Rim15p was identified previously as a stimulator of meiotic gene expression. Here, we show that loss of Rim15p causes an additional pleiotropic phenotype in cells grown to stationary phase on rich medium; this phenotype includes defects in trehalose and glycogen accumulation...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.12.18.2943
更新日期：1998-09-15 00:00:00
abstract：:A long-standing model postulates that X-chromosome dosage compensation in Drosophila occurs by twofold up-regulation of the single male X, but previous data cannot exclude an alternative model, in which male autosomes are down-regulated to balance gene expression. To distinguish between the two models, we used RNA int...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1343705
更新日期：2005-10-01 00:00:00
abstract：:The fission yeast telomerase RNA (TER1) precursor harbors an intron immediately downstream from its mature 3' end. Unlike most introns, which are removed from precursor RNAs by the spliceosome in two sequential but tightly coupled transesterification reactions, TER1 only undergoes the first cleavage reaction during te...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.212738.112
更新日期：2013-03-15 00:00:00
abstract：:Persistent DNA double-strand breaks and telomeres represent genomic hazards, as they can instigate inappropriate repair reactions. Two recent papers by Oza and colleagues (pp. 912-917) and Schober and colleagues (pp. 928-938) show that both types of DNA ends are sequestered from bulk DNA by Mps3, a SUN domain protein ...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1805309
更新日期：2009-05-01 00:00:00
abstract：:Lipids play crucial roles in many aspects of glial cell biology, affecting processes ranging from myelin membrane biosynthesis to axo-glial interactions. In order to study the role of lipid metabolism in myelinating glial cells, we specifically deleted in Schwann cells the Lpin1 gene, which encodes the Mg2+-dependent ...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1638008
更新日期：2008-06-15 00:00:00
abstract：:As in many polarized cells, spindle alignment in yeast is essential and cell cycle regulated. A key step that governs spindle alignment is the selective binding of the Kar9 protein to only one of the two spindle pole bodies (SPBs). It has been suggested that cyclin-dependent kinase Cdc28, in complex with cyclin Clb4, ...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.298704
更新日期：2004-07-15 00:00:00
