Abstract:
:Different RNA species are exported from the nucleus by distinct mechanisms. Among the different RNAs, mRNAs and major spliceosomal U snRNAs share several structural similarities, yet they are exported by distinct factors. We previously showed that U1 snRNAs behaved like an mRNA in nuclear export if various approximately 300-nucleotide fragments were inserted in a central position. Here we show that this export switch is dependent on the length of the insertion but independent of its position, indicating unequivocally that this switch is indeed the result of RNA length. We also show that intronless mRNAs can be progressively converted to use the U snRNA export pathway if the mRNAs are progressively shortened by deletion. In addition, immunoprecipitation experiments show that the protein composition of export RNPs is influenced by RNA length. These findings indicate that RNA length is one of the key determinants of the choice of RNA export pathway. Based on these results and previous observations, a unified model of how an RNA is committed to a specific export pathway is proposed.
journal_name
Genes Devjournal_title
Genes & developmentauthors
Masuyama K,Taniguchi I,Kataoka N,Ohno Mdoi
10.1101/gad.1216204subject
Has Abstractpub_date
2004-09-01 00:00:00pages
2074-85issue
17eissn
0890-9369issn
1549-5477pii
1216204journal_volume
18pub_type
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