Abstract:
:A novel histone methyltransferase, termed Set9, was isolated from human cells. Set9 contains a SET domain, but lacks the pre- and post-SET domains. Set9 methylates specifically lysine 4 (K4) of histone H3 (H3-K4) and potentiates transcription activation. The histone H3 tail interacts specifically with the histone deacetylase NuRD complex. Methylation of histone H3-K4 by Set9 precludes the association of NuRD with the H3 tail. Moreover, methylation of H3-K4 impairs Suv39h1-mediated methylation at K9 of H3 (H3-K9). The interplay between the Set9 and Suv39h1 histone methyltransferases is specific, as the methylation of H3-K9 by the histone methyltransferase G9a was not affected by Set9 methylation of H3-K4. Our studies suggest that Set9-mediated methylation of H3-K4 functions in transcription activation by competing with histone deacetylases and by precluding H3-K9 methylation by Suv39h1. Our results suggest that the methylation of histone tails can have distinct effects on transcription, depending on its chromosomal location, the combination of posttranslational modifications, and the enzyme (or protein complex) involved in the particular modification.
journal_name
Genes Devjournal_title
Genes & developmentauthors
Nishioka K,Chuikov S,Sarma K,Erdjument-Bromage H,Allis CD,Tempst P,Reinberg Ddoi
10.1101/gad.967202subject
Has Abstractpub_date
2002-02-15 00:00:00pages
479-89issue
4eissn
0890-9369issn
1549-5477journal_volume
16pub_type
杂志文章abstract::Wingless (Wg) is an important signaling molecule in the development of Drosophila, but little is known about its signal transduction pathway. Genetic evidence indicates that another segment polarity gene, dishevelled (dsh) is required for Wg signaling. We have recently developed a cell culture system for Wg protein ac...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.9.9.1087
更新日期:1995-05-01 00:00:00
abstract::Telomerase is a ribonucleoprotein enzyme that adds telomeric repeats to chromosomes, maintaining telomere length and stabilizing chromosome ends. In vitro, telomerase from the ciliate Tetrahymena elongates single-stranded, guanosine-rich DNA primers by adding repeats of the Tetrahymena telomeric sequence, dT2G4. We ha...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.7.7b.1364
更新日期:1993-07-01 00:00:00
abstract::Chromatin structure is believed to be important for a number of cellular processes, including transcription. However, the role of nucleosomes in transcription is not well understood. We have identified the yeast histone locus HTB1-HTB1, encoding histones H2A and H2B, as a suppressor of solo delta insertion mutations t...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.2.2.150
更新日期:1988-02-01 00:00:00
abstract::Signaling by the hormones brassinosteroid (BR) and gibberellin (GA) is critical to normal plant growth and development and is required for hypocotyl elongation in response to dark and elevated temperatures. Active BR signaling is essential for GA promotion of hypocotyl growth and suppresses the dwarf phenotype of GA m...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.243675.114
更新日期:2014-08-01 00:00:00
abstract::Numerous lines of study have suggested that the Hox genes, encoding putative transcription factors, are key genes in the establishment of the body plan of the mammalian embryo. To examine the role of Hoxa-5 (Hox1.3) gene during development, we have used targeted mutagenesis in embryonic stem cells to produce a strain ...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.7.11.2085
更新日期:1993-11-01 00:00:00
abstract::Intracellular heme levels must be tightly regulated to maintain proper mitochondrial respiration while minimizing toxicity, but the homeostatic mechanisms are not well understood. Here we report a novel negative feedback mechanism whereby the nuclear heme receptor Rev-erbalpha tightly controls the level of its own lig...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.1825809
更新日期:2009-09-15 00:00:00
abstract::In meiosis I, homologous chromosomes pair and then attach to the spindle so that the homologs can be pulled apart at anaphase I. The segregation of homologs before pairing would be catastrophic. We describe two mechanisms that prevent this. First, in early meiosis, Ipl1, the budding yeast homolog of the mammalian Auro...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.227454.113
更新日期:2013-10-01 00:00:00
abstract::Metazoans start embryogenesis with a relatively naïve genome. The transcriptionally inert, late-replicating heterochromatic regions, including the constitutive heterochromatin on repetitive sequences near centromeres and telomeres, need to be re-established during development. To explore the events initiating heteroch...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.272237.115
更新日期:2016-03-01 00:00:00
abstract::A relevant, yet little recognized feature of antisense regulation is the possibility of switching roles between regulatory and regulated RNAs. Here we show that induction of a Salmonella gene relies on the conversion of a small RNA from effector to regulatory target. The chiP gene (formerly ybfM), identified and chara...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.541609
更新日期:2009-09-01 00:00:00
abstract::The Snail zinc-finger transcription factors trigger epithelial-mesenchymal transitions (EMTs), endowing epithelial cells with migratory and invasive properties during both embryonic development and tumor progression. During EMT, Snail provokes the loss of epithelial markers, as well as changes in cell shape and the ex...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.294104
更新日期:2004-05-15 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.10.3.289
更新日期:1996-02-01 00:00:00
abstract::Programmed cell death occurs in a highly reproducible manner during Caenorhabditis elegans development. We demonstrate that, during embryogenesis, miR-35 and miR-58 bantam family microRNAs (miRNAs) cooperate to prevent the precocious death of mothers of cells programmed to die by repressing the gene egl-1, which encod...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.288555.116
更新日期:2017-01-15 00:00:00
abstract::The human RNA polymerase II (RNAPII)-associated factor complex (hPAFc) and its individual subunits have been implicated in human diseases, including cancer. However, its involvement in breast cancer awaits investigation. Using data mining and human breast cancer tissue microarrays, we found that Ctr9, the key scaffold...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.268722.115
更新日期:2015-10-15 00:00:00
abstract::The embryonic expression of COUP-TFII, an orphan nuclear receptor, suggests that it may participate in mesenchymal-epithelial interactions required for organogenesis. Targeted deletion of the COUP-TFII gene results in embryonic lethality with defects in angiogenesis and heart development. COUP-TFII mutants are defecti...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.13.8.1037
更新日期:1999-04-15 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.1148404
更新日期:2004-02-01 00:00:00
abstract::U4 and U6 small nuclear RNAs are associated by an extensive base-pairing interaction that must be disrupted and reformed with each round of splicing. U4 mutations within the U4/U6 interaction domain destabilize the complex in vitro and cause a cold-sensitive phenotype in vivo. Restabilization of the U4/U6 helix by dom...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.5.5.773
更新日期:1991-05-01 00:00:00
abstract::Sexual identity in Drosophila is determined by zygotic X-chromosome dose. Two potent indicators of X-chromosome dose are sisterless-a (sis-a) and sisterless-b (sis-b). Genetic analysis has shown that a diplo-X dose of these genes activates their regulatory target, the feminizing switch gene Sex-lethal (Sxl), whereas a...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.7.9.1688
更新日期:1993-09-01 00:00:00
abstract::B-cell-specific transcription of immunoglobulin genes is mediated by the interaction of a POU domain containing transcription factor Oct-1 or Oct-2, with the B-cell-specific coactivator OCA-B (Bob-1, OBF-1) and a prototype octamer element. We find that OCA-B binds DNA directly in the major groove between the two subdo...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.10.16.2079
更新日期:1996-08-15 00:00:00
abstract::Unrepaired DNA lesions in the template strand block the replication fork. In yeast, Mec1 protein kinase-mediated replication checkpoint prevents the breakdown of replication forks and maintains viability in DNA-damaged cells going through the S phase. By ensuring that the replisome does not dissociate from the fork st...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.1793409
更新日期:2009-06-15 00:00:00
abstract::Kinetochores play an essential role in chromosome segregation by forming dynamic connections with spindle microtubules. Here, we identify a set of 10 copurifying kinetochore proteins from Caenorhabditis elegans, seven of which were previously uncharacterized. Using in vivo assays to monitor chromosome segregation, kin...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.1234104
更新日期:2004-09-15 00:00:00
abstract::Direct reprogramming of somatic cells into induced pluripotent stem cells (iPSCs) provides a unique opportunity to derive patient-specific stem cells with potential applications in tissue replacement therapies and without the ethical concerns of human embryonic stem cells (hESCs). However, cellular senescence, which c...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.173922.111
更新日期:2011-11-01 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.12.21.3331
更新日期:1998-11-01 00:00:00
abstract::This year is the centenary of Edward G. Conklin's signal findings in embryology: the elucidation of complete cell lineages and the discovery of localized maternal determinants. Conklin used ascidian embryos to elucidate universal principles in embryology. A century later, ascidians, or sea squirts, have not only enter...
journal_title:Genes & development
pub_type: 杂志文章,评审
doi:10.1101/gad.1365805
更新日期:2005-10-15 00:00:00
abstract::The histone variants H3.3 and H2A.Z have recently emerged as two of the most important features in transcriptional regulation, the molecular mechanism of which still remains poorly understood. In this study, we investigated the regulation of H3.3 and H2A.Z on chromatin dynamics during transcriptional activation. Our i...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.222174.113
更新日期:2013-10-01 00:00:00
abstract::The gene for an essential protein subunit of nuclear RNase P from Saccharomyces cerevisiae has been cloned. The gene for this protein, RPP1, was identified by virtue of its homology with a human scleroderma autoimmune antigen, Rpp30, which copurifies with human RNase P. Epitope-tagged Rpp1 can be found in association ...
journal_title:Genes & development
pub_type: 更正并重新发布的文章,杂志文章
doi:10.1101/gad.11.21.2926
更新日期:1997-11-01 00:00:00
abstract::Pre-rRNA transcription by RNA Polymerase I (Pol I) is very robust on active rDNA repeats. Loss of yeast Topoisomerase I (Top1) generated truncated pre-rRNA fragments, which were stabilized in strains lacking TRAMP (Trf4/Trf5-Air1/Air2-Mtr4 polyadenylation complexes) or exosome degradation activities. Loss of both Top1...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.573310
更新日期:2010-07-15 00:00:00
abstract::Genetic screening and selection procedures employing a secA-lacZ fusion strain repeatedly have yielded mutations in four genes affecting the protein export pathway of Escherichia coli. These genes are secA, secD, prlA/secY, and secE. We discuss the significance of the failure to find new sec genes after extensive use ...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.3.7.1035
更新日期:1989-07-01 00:00:00
abstract::Chk2/hcds1, the human homolog of the Saccharomyces cerevisiae RAD53/SPK1 and Schizosaccharomyces pombe cds1 DNA damage checkpoint genes, encodes a protein kinase that is post-translationally modified after DNA damage. Like its yeast homologs, the Chk2/hCds1 protein phosphorylates Cdc25C in vitro, suggesting that it ar...
journal_title:Genes & development
pub_type: 杂志文章
doi:
更新日期:2000-02-01 00:00:00
abstract::During vasculogenesis and angiogenesis, endothelial cell responses to growth factors are modulated by the compositional and mechanical properties of a surrounding three-dimensional (3D) extracellular matrix (ECM) that is dominated by either cross-linked fibrin or type I collagen. While 3D-embedded endothelial cells es...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.1643308
更新日期:2008-05-01 00:00:00
abstract::The growth suppression function of the retinoblastoma protein (RB) is mediated by its interaction with a variety of cellular proteins. RB contains at least two protein-binding pockets: the large A/B pocket, which interacts with E2F and the D-type cyclins, and the C pocket, which interacts with the nuclear c-Abl tyrosi...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.9.1.31
更新日期:1995-01-01 00:00:00