Abstract：
:In vivo experiments have demonstrated that the ribosomal protein L32 of Saccharomyces cerevisiae brings about the inhibition of splicing of the transcript of its own gene through an RNA structure comprised largely of the first exon. We now show that L32, itself, binds specifically to this RNA. Splicing of the RPL32 transcript in vitro is blocked by the presence of L32. Furthermore, addition of the 75-nucleotide RNA representing the 5' end of the RPL32 transcript stimulates specifically the splicing of the RPL32 substrate, presumably by competing for L32 present in the extract. Use of RNAs carrying mutations shown to abolish the regulation of splicing, either as substrates or as competitors, confirmed that the in vitro reaction is a faithful representation of the situation in vivo. We conclude that the regulation of splicing occurs through the specific binding of L32 to an RNA structure within the first 75 nucleotides of the RPL32 transcript. The RPL32 substrate, bound to L32, forms a complex with U1 snRNP, the first step in spliceosome assembly. The presence of L32 prevents the ATP-dependent association of the U2 snRNP necessary to form a complete spliceosome.
journal_name
Genes Devjournal_title
Genes & developmentauthors
Vilardell J,Warner JRdoi
10.1101/gad.8.2.211subject
Has Abstractpub_date
1994-01-01 00:00:00pages
211-20issue
2eissn
0890-9369issn
1549-5477journal_volume
8pub_type
杂志文章abstract：:The discovery of transcription factors (TFs) controlling pathways in health and disease is of paramount interest. We designed a widely applicable method, dubbed barcorded synthetic tandem repeat promoter screening (BC-STAR-PROM), to identify signal-activated TFs without any a priori knowledge about their properties. T...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.284828.116
更新日期：2016-08-15 00:00:00
abstract：:The empty spiracles (ems) homeo box gene is required for the development of the Drosophila larval filzkörper, which are structural specializations of the eighth abdominal segment. Filzkörper development is also dependent on the function of the homeotic selector gene Abdominal-B (Abd-B). Here, we show that ems is a dow...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.7.2.229
更新日期：1993-02-01 00:00:00
abstract：:A major class of bacterial small RNAs (sRNAs), along with RNA-binding protein Hfq and endoribonuclease RNase E, acts on target mRNAs through base-pairing, leading to translational repression and rapid degradation of the mRNAs. In this issue of Genes & Development, Prévost and colleagues (pp. 385-396) demonstrate by us...
journal_title：Genes & development
pub_type： 评论,杂志文章
doi：10.1101/gad.2030311
更新日期：2011-02-15 00:00:00
abstract：:Cell differentiation is essential for the development of multicellular organisms. In flowering plants, the haploid male gametophytes (pollen grains) are generated in the anther from reproductive cells called microsporocytes. Several types of somatic cells ensure successful pollen development, and thus reproduction. Ho...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.997902
更新日期：2002-08-01 00:00:00
abstract：:Our understanding of eukaryotic transcriptional activation mechanisms has been hampered by an inability to identify the direct in vivo targets of activator proteins, primarily because of lack of appropriate experimental methods. To circumvent this problem, we have developed a fluorescence resonance energy transfer (FR...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1148404
更新日期：2004-02-01 00:00:00
abstract：:The regulation of gene transcription is critical for the proper development and growth of an organism. The transcription of protein-coding genes initiates at the RNA polymerase II core promoter, which is a diverse module that can be controlled by many different elements such as the TATA box and downstream core promote...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1698108
更新日期：2008-10-15 00:00:00
abstract：:Homeotic genes are expressed in spatially precise patterns during Drosophila development to control segmental differentiation. The Sex combs reduced (Scr) gene of the Antennapedia gene complex is involved in the determination of the labial and prothoracic segments of the embryo. To study both the wild-type pattern of ...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1.7.716
更新日期：1987-09-01 00:00:00
abstract：:Genomic imprinting, an inherently epigenetic phenomenon defined by parent of origin-dependent gene expression, is observed in mammals and flowering plants. Genome-scale surveys of imprinted expression and the underlying differential epigenetic marks have led to the discovery of hundreds of imprinted plant genes and co...
journal_title：Genes & development
pub_type： 杂志文章,评审
doi：10.1101/gad.269902.115
更新日期：2015-12-15 00:00:00
abstract：:The embryonic lethal abnormal visual system (ELAV) is a gene-specific regulator of alternative pre-mRNA processing in neurons of Drosophila. Here we define a functional in vivo binding site for ELAV in neurons through the development of a reporter gene system in transgenic animals in combination with in vitro binding ...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1106703
更新日期：2003-10-15 00:00:00
abstract：:Transcriptional activation of the human U1 snRNA genes is dependent on a noncanonical octamer element contained within an upstream enhancer. The U1 octamer only weakly recruits the Oct-1 POU domain, although recruitment is stimulated by a peptide containing the Oct-1-binding domain of SNAP190. Structural analysis of t...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1021002
更新日期：2002-11-01 00:00:00
abstract：:The ECS (Elongin B/C-Cul2/Cul5-SOCS-box protein) complex is a member of a family of ubiquitin ligases that share a Cullin-Rbx module. SOCS-box proteins recruit substrates to the ECS complex and are linked to Cullin-Rbx via Elongin B/C. VHL has been implicated as a SOCS-box protein, but lacks a C-terminal sequence (dow...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1252404
更新日期：2004-12-15 00:00:00
abstract：:Numerous lines of study have suggested that the Hox genes, encoding putative transcription factors, are key genes in the establishment of the body plan of the mammalian embryo. To examine the role of Hoxa-5 (Hox1.3) gene during development, we have used targeted mutagenesis in embryonic stem cells to produce a strain ...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.7.11.2085
更新日期：1993-11-01 00:00:00
abstract：:Cosuppression is a silencing phenomenon triggered by the introduction of homologous DNA sequences into the genomes of organisms as diverse as plants, fungi, flies, and nematodes. Here we report sex-induced silencing (SIS), which is triggered by tandem integration of a transgene array in the human fungal pathogen Crypt...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1970910
更新日期：2010-11-15 00:00:00
abstract：:Two independent two-dimensional (2D) gel methods were used to map an origin of replication that is developmentally regulated by the steroid hormone ecdysone, namely an origin for DNA puff amplification in the fungus fly Sciara coprophila. Initiation of replication was found to occur within a small region of no larger ...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.7.6.1072
更新日期：1993-06-01 00:00:00
abstract：:The P family of transposable elements in Drosophila transpose by a cut-and-paste mechanism involving double-strand gap repair. We report here that a Drosophila mutagen-sensitive mutant, mus3O9, contains a mutation in IRBP (inverted repeat binding protein), the Drosophila homolog of the mammalian Ku p70 gene. We show t...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.10.8.921
更新日期：1996-04-15 00:00:00
abstract：:DNA replication remains unfinished in many Drosophila polyploid cells, which harbor disproportionately fewer copies of late-replicating chromosomal regions. By analyzing paired-end high-throughput sequence data from polytene larval salivary gland cells, we define 112 underreplicated (UR) euchromatic regions 60-480 kb ...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.245811.114
更新日期：2014-08-15 00:00:00
abstract：:Many tumors continuously depend on the initiating oncogenes, but whether this extends to their downstream targets is unclear. In this issue of Genes & Development, Sodir and colleagues (pp. 907-916) demonstrate an essential role for endogenous Myc proteins in maintaining the tumor microenvironment, providing an unexpe...
journal_title：Genes & development
pub_type： 评论,杂志文章
doi：10.1101/gad.2053311
更新日期：2011-05-01 00:00:00
abstract：:Thousands of eukaryotic protein-coding genes are noncanonically spliced to produce circular RNAs. Bioinformatics has indicated that long introns generally flank exons that circularize in Drosophila, but the underlying mechanisms by which these circular RNAs are generated are largely unknown. Here, using extensive muta...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.270421.115
更新日期：2015-10-15 00:00:00
abstract：:Chromosome replication occurs precisely once during the cell cycle of almost all eukaryotic cells, and is a highly complex process that is still understood relatively poorly. Two conserved kinases called Cdc7 (cell division cycle 7) and cyclin-dependent kinase (CDK) are required to establish replication forks during t...
journal_title：Genes & development
pub_type： 杂志文章,评审
doi：10.1101/gad.1933010
更新日期：2010-06-15 00:00:00
abstract：:In Saccharomyces cerevisiae, gene silencing at the HMR and HML loci is normally dependent on Sir2p, Sir3p, and Sir4p, which are structural components of silenced chromatin. Sir2p is a NAD+-dependent histone deacetylase required for silencing. Silencing can be restored in cells lacking Sir proteins by a dominant mutati...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.873601
更新日期：2001-04-15 00:00:00
abstract：:Somitogenesis involves the segmentation of the paraxial mesoderm into units along the anteroposterior axis. Here we show a role for Eph and ephrin signaling in the patterning of presomitic mesoderm and formation of the somites. Ephrin-A-L1 and ephrin-B2 are expressed in an iterative manner in the developing somites an...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.12.19.3096
更新日期：1998-10-01 00:00:00
abstract：:Chk2/hcds1, the human homolog of the Saccharomyces cerevisiae RAD53/SPK1 and Schizosaccharomyces pombe cds1 DNA damage checkpoint genes, encodes a protein kinase that is post-translationally modified after DNA damage. Like its yeast homologs, the Chk2/hCds1 protein phosphorylates Cdc25C in vitro, suggesting that it ar...
journal_title：Genes & development
pub_type： 杂志文章
doi：
更新日期：2000-02-01 00:00:00
abstract：:Wingless (Wg) is an important signaling molecule in the development of Drosophila, but little is known about its signal transduction pathway. Genetic evidence indicates that another segment polarity gene, dishevelled (dsh) is required for Wg signaling. We have recently developed a cell culture system for Wg protein ac...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.9.9.1087
更新日期：1995-05-01 00:00:00
abstract：:DinI is a recently described negative regulator of the SOS response in Escherichia coli. Here we show that it physically interacts with RecA and prevents the binding of single-stranded DNA to RecA, which is required for the activation of the latter. DinI also displaces ssDNA from a stable RecA-DNA cofilament, thus eli...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.862901
更新日期：2001-02-15 00:00:00
abstract：:Caenorhabditis elegans let-7, a founding member of the microRNA family, is predicted to bind to six sites in the 3'UTR of the mRNA of its target gene, lin-41, to down-regulate LIN-41. Here, we demonstrate that wild-type let-7 microRNA binds in vitro to RNA from the lin-41 3'UTR. This interaction is dependent on two co...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1165404
更新日期：2004-01-15 00:00:00
abstract：:Genome instability is a recurring feature of tumorigenesis. Mutation in MLL2, encoding a histone methyltransferase, is a driver in numerous different cancer types, but the mechanism is unclear. Here, we present evidence that MLL2 mutation results in genome instability. Mouse cells in which MLL2 gene deletion can be in...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.275453.115
更新日期：2016-02-15 00:00:00
abstract：:Evidence is presented that the activation of the RNA polymerase sigma factor sigma(W) in Bacillus subtilis by regulated intramembrane proteolysis is governed by a novel, membrane-embedded protease. The sigma(W) factor is activated by proteolytic destruction of the membrane-bound anti-sigma(W) factor RsiW in response t...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1440606
更新日期：2006-07-15 00:00:00
abstract：:Previous work has shown that rRNA synthesis is strongly inhibited in yeast top1-top2 double mutants. Here, we show that inactivation of yeast topoisomerases can have paradoxical effects on transcription by RNA polymerase I. For example, transcription of ribosomal minigenes on extrachromosomal plasmids is greatly stimu...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.6.7.1332
更新日期：1992-07-01 00:00:00
abstract：:Mammalian development requires effective mechanisms to repress genes whose expression would generate inappropriately specified cells. The Polycomb-repressive complex 1 (PRC1) family complexes are central to maintaining this repression. These include a set of canonical PRC1 complexes, each of which contains four core p...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.326488.119
更新日期：2019-07-01 00:00:00
abstract：:The organizer region, or dorsal blastopore lip, plays a central role in the initiation of gastrulation and the formation of the body axis during Xenopus development. A similar process can also be induced in ectodermal explants by activin or by injection of activin mRNA into embryos. We have searched early embryo-speci...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.6.4.599
更新日期：1992-04-01 00:00:00
