RNase E action at a distance: degradation of target mRNAs mediated by an Hfq-binding small RNA in bacteria.


:A major class of bacterial small RNAs (sRNAs), along with RNA-binding protein Hfq and endoribonuclease RNase E, acts on target mRNAs through base-pairing, leading to translational repression and rapid degradation of the mRNAs. In this issue of Genes & Development, Prévost and colleagues (pp. 385-396) demonstrate by using the well-characterized sRNA RyhB that RNase E cleavage at sites distal from the pairing region triggers degradation of target mRNAs. The study has provided an important insight into the initial events of sRNA-induced degradation of target mRNAs.


Genes Dev


Genes & development


Morita T,Aiba H




Has Abstract


2011-02-15 00:00:00














  • Defective N-sulfation of heparan sulfate proteoglycans limits PDGF-BB binding and pericyte recruitment in vascular development.

    abstract::During vascular development, endothelial platelet-derived growth factor B (PDGF-B) is critical for pericyte recruitment. Deletion of the conserved C-terminal heparin-binding motif impairs PDGF-BB retention and pericyte recruitment in vivo, suggesting a potential role for heparan sulfate (HS) in PDGF-BB function during...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Abramsson A,Kurup S,Busse M,Yamada S,Lindblom P,Schallmeiner E,Stenzel D,Sauvaget D,Ledin J,Ringvall M,Landegren U,Kjellén L,Bondjers G,Li JP,Lindahl U,Spillmann D,Betsholtz C,Gerhardt H

    更新日期:2007-02-01 00:00:00

  • Radical mutations reveal TATA-box binding protein surfaces required for activated transcription in vivo.

    abstract::Regions on the surface of human TATA-box binding protein (TBP) required for activated transcription in vivo were defined by construction of a library of 89 surface residue mutants with radical substitutions that were assayed for their ability to support activated transcription in vivo, basal transcription in vitro, an...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Bryant GO,Martel LS,Burley SK,Berk AJ

    更新日期:1996-10-01 00:00:00

  • MEK-2, a Caenorhabditis elegans MAP kinase kinase, functions in Ras-mediated vulval induction and other developmental events.

    abstract::Activated Ras initiates a cascade of sequential phosphorylation events, including the protein kinases Raf, MEK, and MAP kinase. The Let-60 Ras-mediated signal transduction pathway controls vulval induction in Caenorhabditis elegans. Both Lin-45 Raf and Sur-1 MAP kinase have been determined to be essential factors duri...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Wu Y,Han M,Guan KL

    更新日期:1995-03-15 00:00:00

  • Transcript levels and translational control of hsp70 synthesis in Xenopus oocytes.

    abstract::Until recently it was believed that Xenopus oocytes respond to heat shock by synthesizing the 70-kD heat shock protein hsp70 and that, uniquely amongst animal cell types, this response is mediated entirely at the translational level. This view has now been challenged and we present data that reevaluate the involvement...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Horrell A,Shuttleworth J,Colman A

    更新日期:1987-07-01 00:00:00

  • SCH9, a gene of Saccharomyces cerevisiae that encodes a protein distinct from, but functionally and structurally related to, cAMP-dependent protein kinase catalytic subunits.

    abstract::A new gene, SCH9, was isolated from Saccharomyces cerevisiae by its ability to complement a cdc25ts mutation. Sequence analysis indicates that it encodes a 90,000-dalton protein with a carboxy-terminal domain homologous to yeast and mammalian cAMP-dependent protein kinase catalytic subunits. In addition to suppressing...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Toda T,Cameron S,Sass P,Wigler M

    更新日期:1988-05-01 00:00:00

  • Mice lacking progesterone receptor exhibit pleiotropic reproductive abnormalities.

    abstract::Although progesterone has been recognized as essential for the establishment and maintenance of pregnancy, this steroid hormone has been recently implicated to have a functional role in a number of other reproductive events. The physiological effects of progesterone are mediated by the progesterone receptor (PR), a me...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Lydon JP,DeMayo FJ,Funk CR,Mani SK,Hughes AR,Montgomery CA Jr,Shyamala G,Conneely OM,O'Malley BW

    更新日期:1995-09-15 00:00:00

  • ELAV tumor antigen, Hel-N1, increases translation of neurofilament M mRNA and induces formation of neurites in human teratocarcinoma cells.

    abstract::Human ELAV proteins are implicated in cell growth and differentiation via regulation of mRNA expression in the cytoplasm. In human embryonic teratocarcinoma (hNT2) cells transfected with the human neuronal ELAV-like protein, Hel-N1, neurites formed, yet cells were not terminally differentiated. Cells in which neurite ...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Antic D,Lu N,Keene JD

    更新日期:1999-02-15 00:00:00

  • Dynamic association of proteins with the pre-mRNA branch region.

    abstract::The association of proteins with the branch site region during pre-mRNA splicing was probed using a novel methodology to site-specifically modify the pre-mRNA with the photo-reagent benzophenone. Three sets of proteins were distinguished by the kinetics of their associations with pre-mRNAs, by their association with d...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: MacMillan AM,Query CC,Allerson CR,Chen S,Verdine GL,Sharp PA

    更新日期:1994-12-15 00:00:00

  • Mechanistic insights and identification of two novel factors in the C. elegans NMD pathway.

    abstract::The nonsense-mediated mRNA decay (NMD) pathway selectively degrades mRNAs harboring premature termination codons (PTCs). Seven genes (smg-1-7, for suppressor with morphological effect on genitalia) that are essential for NMD were originally identified in the nematode Caenorhabditis elegans, and orthologs of these gene...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Longman D,Plasterk RH,Johnstone IL,Cáceres JF

    更新日期:2007-05-01 00:00:00

  • The flagellar-specific transcription factor, sigma28, is the Type III secretion chaperone for the flagellar-specific anti-sigma28 factor FlgM.

    abstract::The sigma(28) protein is a member of the bacterial sigma(70)-family of transcription factors that directs RNA polymerase to flagellar late (class 3) promoters. The sigma(28) protein is regulated in response to flagellar assembly by the anti-sigma(28) factor FlgM. FlgM inhibits sigma(28)-dependent transcription of gene...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Aldridge PD,Karlinsey JE,Aldridge C,Birchall C,Thompson D,Yagasaki J,Hughes KT

    更新日期:2006-08-15 00:00:00

  • N-myc alters the fate of preneoplastic cells in a mouse model of medulloblastoma.

    abstract::Studying the early stages of cancer can provide important insight into the molecular basis of the disease. We identified a preneoplastic stage in the patched (ptc) mutant mouse, a model for the brain tumor medulloblastoma. Preneoplastic cells (PNCs) are found in most ptc mutants during early adulthood, but only 15% of...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Kessler JD,Hasegawa H,Brun SN,Emmenegger BA,Yang ZJ,Dutton JW,Wang F,Wechsler-Reya RJ

    更新日期:2009-01-15 00:00:00

  • Retinoic acid modifies mesodermal patterning in early Xenopus embryos.

    abstract::Treatment of early Xenopus embryos with retinoic acid (RA) produces a concentration-dependent series of defects in anterior axial structures that range from small deletions to embryos lacking heads. The graded series of axial defects obtained after RA administration to early embryos appears to result, in part, from ac...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Ruiz i Altaba A,Jessell T

    更新日期:1991-02-01 00:00:00

  • Smad3-dependent nuclear translocation of beta-catenin is required for TGF-beta1-induced proliferation of bone marrow-derived adult human mesenchymal stem cells.

    abstract::Adult mesenchymal stem cells (MSCs) derived from bone marrow contribute to the regeneration of multiple types of mesenchymal tissues. Here we describe the functional role of a novel form of cross-talk between the transforming growth factor beta1 (TGF-beta1) and Wnt signaling pathways in regulating the activities of hu...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Jian H,Shen X,Liu I,Semenov M,He X,Wang XF

    更新日期:2006-03-15 00:00:00

  • Sorting out adipocyte precursors and their role in physiology and disease.

    abstract::The ability to maintain and expand the pool of adipocytes in adults is integral to the regulation of energy balance, tissue/stem cell homeostasis, and disease pathogenesis. For decades, our knowledge of adipocyte precursors has relied on cellular models. The identity of native adipocyte precursors has remained unclear...

    journal_title:Genes & development

    pub_type: 杂志文章,评审


    authors: Hepler C,Vishvanath L,Gupta RK

    更新日期:2017-01-15 00:00:00

  • The efficiency of RNA 3'-end formation is determined by the distance between the cap site and the poly(A) site in spleen necrosis virus.

    abstract::The efficiency of RNA 3'-end formation of spleen necrosis virus (SNV) is determined by the distance between the cap site and the poly(A) site. When the distance between the cap site and the poly(A) site was shorter than 500 bases, only 3-9% of the RNA was polyadenylated at the SNV poly(A) site. However, when the dista...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Iwasaki K,Temin HM

    更新日期:1990-12-01 00:00:00

  • rhomboid, a gene required for dorsoventral axis establishment and peripheral nervous system development in Drosophila melanogaster.

    abstract::rhomboid (rho) belongs to a group of four genes involved in the elaboration of positional information at a ventrolateral level in the Drosophila embryo. Mutations at any of these four loci also lead to a variety of other phenotypes, including reduction in the number of stretch receptor organs (chordotonal organs) in t...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Bier E,Jan LY,Jan YN

    更新日期:1990-02-01 00:00:00

  • The transcriptional activator PAX3-FKHR rescues the defects of Pax3 mutant mice but induces a myogenic gain-of-function phenotype with ligand-independent activation of Met signaling in vivo.

    abstract::Pax3 is a key transcription factor implicated in development and human disease. To dissect the role of Pax3 in myogenesis and establish whether it is a repressor or activator, we generated loss- and gain-of-function alleles by targeting an nLacZ reporter and a sequence encoding the oncogenic fusion protein PAX3-FKHR i...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Relaix F,Polimeni M,Rocancourt D,Ponzetto C,Schäfer BW,Buckingham M

    更新日期:2003-12-01 00:00:00

  • Masquerade: a novel secreted serine protease-like molecule is required for somatic muscle attachment in the Drosophila embryo.

    abstract::Diverse developmental processes, such as neuronal growth cone migration and cell shape changes, are mediated by the interactions of cells with the extracellular matrix. We describe here a secreted molecule encoded by the Drosophila masquerade (mas) gene. Total loss of mas function causes defective muscle attachment. T...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Murugasu-Oei B,Rodrigues V,Yang X,Chia W

    更新日期:1995-01-15 00:00:00

  • Homozygosity for prion protein alleles encoding glutamine-171 renders sheep susceptible to natural scrapie.

    abstract::Natural scrapie has been viewed both as a recessive trait and as a contagious disease modulated by a host locus. To address this conundrum, we determined the structure of the sheep prion protein (PrP) gene, which contains three exons and extends over 20 kb of DNA. In the United States 86.4% of scrapie cases occur in S...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Westaway D,Zuliani V,Cooper CM,Da Costa M,Neuman S,Jenny AL,Detwiler L,Prusiner SB

    更新日期:1994-04-15 00:00:00

  • Tetrahymena telomerase catalyzes nucleolytic cleavage and nonprocessive elongation.

    abstract::Telomerase is a ribonucleoprotein enzyme that adds telomeric repeats to chromosomes, maintaining telomere length and stabilizing chromosome ends. In vitro, telomerase from the ciliate Tetrahymena elongates single-stranded, guanosine-rich DNA primers by adding repeats of the Tetrahymena telomeric sequence, dT2G4. We ha...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Collins K,Greider CW

    更新日期:1993-07-01 00:00:00

  • Transcription factor Ebf1 regulates differentiation stage-specific signaling, proliferation, and survival of B cells.

    abstract::The transcription factor Ebf1 is an important determinant of early B lymphopoiesis. To gain insight into the functions of Ebf1 at distinct stages of differentiation, we conditionally inactivated Ebf1. We found that Ebf1 is required for the proliferation, survival, and signaling of pro-B cells and peripheral B-cell sub...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Györy I,Boller S,Nechanitzky R,Mandel E,Pott S,Liu E,Grosschedl R

    更新日期:2012-04-01 00:00:00

  • Set9, a novel histone H3 methyltransferase that facilitates transcription by precluding histone tail modifications required for heterochromatin formation.

    abstract::A novel histone methyltransferase, termed Set9, was isolated from human cells. Set9 contains a SET domain, but lacks the pre- and post-SET domains. Set9 methylates specifically lysine 4 (K4) of histone H3 (H3-K4) and potentiates transcription activation. The histone H3 tail interacts specifically with the histone deac...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Nishioka K,Chuikov S,Sarma K,Erdjument-Bromage H,Allis CD,Tempst P,Reinberg D

    更新日期:2002-02-15 00:00:00

  • Rev-erbα and Rev-erbβ coordinately protect the circadian clock and normal metabolic function.

    abstract::The nuclear receptor Rev-erbα regulates circadian rhythm and metabolism, but its effects are modest and it has been considered to be a secondary regulator of the cell-autonomous clock. Here we report that depletion of Rev-erbα together with closely related Rev-erbβ has dramatic effects on the cell-autonomous clock as ...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Bugge A,Feng D,Everett LJ,Briggs ER,Mullican SE,Wang F,Jager J,Lazar MA

    更新日期:2012-04-01 00:00:00

  • Conversion of neurons and glia to external-cell fates in the external sensory organs of Drosophila hamlet mutants by a cousin-cousin cell-type respecification.

    abstract::The Drosophila external sensory organ forms in a lineage elaborating from a single precursor cell via a stereotypical series of asymmetric divisions. HAMLET transcription factor expression demarcates the lineage branch that generates two internal cell types, the external sensory neuron and thecogen. In HAMLET mutant o...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Moore AW,Roegiers F,Jan LY,Jan YN

    更新日期:2004-03-15 00:00:00

  • 3'-end formation of mouse pre-rRNA involves both transcription termination and a specific processing reaction.

    abstract::We have studied the sequence requirements for 3'-end formation of rDNA transcripts in a cell-free system and show that the generation of correct ends of mouse pre-rRNA is brought about by a two-step process that involves a bona fide termination reaction, followed by a specific trimming of the primary transcript by 10 ...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Kuhn A,Grummt I

    更新日期:1989-02-01 00:00:00

  • Placental cell fates are regulated in vivo by HIF-mediated hypoxia responses.

    abstract::Placental development is profoundly influenced by oxygen (O(2)) tension. Human cytotrophoblasts proliferate in vitro under low O(2) conditions but differentiate at higher O(2) levels, mimicking the developmental transition they undergo as they invade the placental bed to establish the maternal-fetal circulation in viv...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Adelman DM,Gertsenstein M,Nagy A,Simon MC,Maltepe E

    更新日期:2000-12-15 00:00:00

  • Evidence for instability of mRNAs containing AUUUA motifs mediated through translation-dependent assembly of a > 20S degradation complex.

    abstract::Many short-lived mRNAs, including those encoding lymphokines, cytokines, and proto-oncogenes, contain an AU-rich sequence in their 3'-untranslated regions. These AU domains and, more specifically, AUUUA motifs within them, are widely thought to mediate the extreme instability of the corresponding mRNAs. This is most c...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Savant-Bhonsale S,Cleveland DW

    更新日期:1992-10-01 00:00:00

  • Selective disruption of genes expressed in totipotent embryonal stem cells.

    abstract::Two retrovirus promoter trap vectors (U3His and U3Neo) have been used to disrupt genes expressed in totipotent murine embryonal stem (ES) cells. Selection in L-histidinol or G418 produced clones in which the coding sequences for histidinol-dehydrogenase or neomycin-phosphotransferase were fused to sequences in or near...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: von Melchner H,DeGregori JV,Rayburn H,Reddy S,Friedel C,Ruley HE

    更新日期:1992-06-01 00:00:00

  • Snail blocks the cell cycle and confers resistance to cell death.

    abstract::The Snail zinc-finger transcription factors trigger epithelial-mesenchymal transitions (EMTs), endowing epithelial cells with migratory and invasive properties during both embryonic development and tumor progression. During EMT, Snail provokes the loss of epithelial markers, as well as changes in cell shape and the ex...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Vega S,Morales AV,Ocaña OH,Valdés F,Fabregat I,Nieto MA

    更新日期:2004-05-15 00:00:00

  • Molecular mechanisms of Spemann's organizer formation: conserved growth factor synergy between Xenopus and mouse.

    abstract::Mesoderm induction assays in Xenopus have implicated growth factors such as activin, Vg1, Xwnt-8, and noggin as important in directing the formation of dorsal mesoderm (Spemann's organizer). Because these growth factors are structurally very different, they presumably act through distinct cell surface receptors that i...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Watabe T,Kim S,Candia A,Rothbächer U,Hashimoto C,Inoue K,Cho KW

    更新日期:1995-12-15 00:00:00