Abstract:
:A major class of bacterial small RNAs (sRNAs), along with RNA-binding protein Hfq and endoribonuclease RNase E, acts on target mRNAs through base-pairing, leading to translational repression and rapid degradation of the mRNAs. In this issue of Genes & Development, Prévost and colleagues (pp. 385-396) demonstrate by using the well-characterized sRNA RyhB that RNase E cleavage at sites distal from the pairing region triggers degradation of target mRNAs. The study has provided an important insight into the initial events of sRNA-induced degradation of target mRNAs.
journal_name
Genes Devjournal_title
Genes & developmentauthors
Morita T,Aiba Hdoi
10.1101/gad.2030311subject
Has Abstractpub_date
2011-02-15 00:00:00pages
294-8issue
4eissn
0890-9369issn
1549-5477pii
25/4/294journal_volume
25pub_type
评论,杂志文章abstract::The cytochrome P450 (CYP) gene products such as CYP3A and CYP2B are essential for the metabolism of steroid hormones and xenochemicals including prescription drugs. Nuclear receptor SXR/PXR (steroid and xenobiotic receptor/pregnenolone X receptor) has been shown both biochemically and genetically to activate CYP3A gen...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.846800
更新日期:2000-12-01 00:00:00
abstract::The Caenorhabditis elegans caudal homolog, pal-1, is required for neurogenesis in the male tail. We show that expression of pal-1 in the postembryonic neuroblast cell V6 can be initiated by two alternate pathways. One pathway, acting in wild type, requires a regulatory element in the fifth pal-1 intron. The other path...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.814700
更新日期:2000-09-01 00:00:00
abstract::Genomic imprinting is essential for mammalian development. Recent studies have revealed that maternal histone H3 Lys27 trimethylation (H3K27me3) can mediate DNA methylation-independent genomic imprinting. However, the regulatory mechanisms and functions of this new imprinting mechanism are largely unknown. Here we dem...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.318675.118
更新日期:2018-12-01 00:00:00
abstract::Deregulated expression of the c-myc proto-oncogene can lead to apoptosis under certain physiological conditions. By introducing a conditionally active Myc allele into primary embryo fibroblasts null for p53, and into fibroblasts without endogenous p53 expression but ectopically expressing a temperature-sensitive p53 a...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.8.23.2817
更新日期:1994-12-01 00:00:00
abstract::Spliceosome assembly has been characterized as the ordered association of the snRNP particles U1, U2, and U4/U6.U5 onto pre-mRNA. We have used an in vitro trans-splicing/cross-linking system in Saccharomyces cerevisiae nuclear extracts to examine the first step of this process, 5' splice site recognition. This trans-s...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.895601
更新日期:2001-08-01 00:00:00
abstract::In Saccharomyces cerevisiae, absence of the checkpoint kinase Mec1 (ATR) is viable upon mutations that increase the activity of the ribonucleotide reductase (RNR) complex. Whether this pathway is conserved in mammals remains unknown. Here we show that cells from mice carrying extra alleles of the RNR regulatory subuni...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.256958.114
更新日期:2015-04-01 00:00:00
abstract::The ECS (Elongin B/C-Cul2/Cul5-SOCS-box protein) complex is a member of a family of ubiquitin ligases that share a Cullin-Rbx module. SOCS-box proteins recruit substrates to the ECS complex and are linked to Cullin-Rbx via Elongin B/C. VHL has been implicated as a SOCS-box protein, but lacks a C-terminal sequence (dow...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.1252404
更新日期:2004-12-15 00:00:00
abstract::Pre-rRNA transcription by RNA Polymerase I (Pol I) is very robust on active rDNA repeats. Loss of yeast Topoisomerase I (Top1) generated truncated pre-rRNA fragments, which were stabilized in strains lacking TRAMP (Trf4/Trf5-Air1/Air2-Mtr4 polyadenylation complexes) or exosome degradation activities. Loss of both Top1...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.573310
更新日期:2010-07-15 00:00:00
abstract::Transcription factors represent one of the largest groups of proteins regulated by SUMO (small ubiquitin-like modifier) modification, and their sumoylation is usually associated with transcriptional repression. To investigate whether sumoylation plays a general role in regulating transcription in yeast, we determined ...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.1917910
更新日期:2010-06-15 00:00:00
abstract::TDP-43 is a critical RNA-binding factor associated with pre-mRNA splicing in mammals. Its expression is tightly autoregulated, with loss of this regulation implicated in human neuropathology. We demonstrate that TDP-43 overexpression in humans and mice activates a 3' untranslated region (UTR) intron, resulting in exci...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.194829.112
更新日期:2012-08-01 00:00:00
abstract::Genomic imprinting is an epigenetic mechanism by which genes are expressed in a parental origin-dependent manner. We recently discovered that, like DNA methylation, oocyte-inherited H3K27me3 can also serve as an imprinting mark in mouse preimplantation embryos. In this study, we found H3K27me3 is strongly biased towar...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.323105.118
更新日期:2019-04-01 00:00:00
abstract::One of the first steps in neurogenesis is the diversification of cells along the dorsoventral axis. In Drosophila the central nervous system develops from three longitudinal columns of cells: ventral cells that express the vnd/nk2 homeobox gene, intermediate cells, and dorsal cells that express the msh homeobox gene. ...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.12.22.3591
更新日期:1998-11-15 00:00:00
abstract::The agouti gene regulates the differential production of eumelanin (black or brown) and phaeomelanin (yellow) pigment granules by melanocytes in the hair follicles of mice. The original nonagouti (a) allele, which confers a predominantly black coat color, has been shown to revert to two other more dominant agouti alle...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.8.4.481
更新日期:1994-02-15 00:00:00
abstract::The histone regulatory (HIR) and histone promoter control (HPC) repressor proteins regulate three of the four histone gene loci during the Saccharomyces cerevisiae cell cycle. Here, we demonstrate that Hir1, Hir2, Hir3, and Hpc2 proteins form a stable HIR repressor complex. The HIR complex promotes histone deposition ...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.1341105
更新日期:2005-11-01 00:00:00
abstract::Transgenic mice expressing wild-type murine p53 under the control of the mouse mammary tumor virus long terminal repeat (MMTV LTR) undergo progressive renal failure due to abnormal kidney development. Similar phenotypes are observed in two transgenic lines that express wild-type p53 within the ureteric bud but not in ...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.10.7.836
更新日期:1996-04-01 00:00:00
abstract::It is postulated that the symmetric organization of plant lateral organs is based on two crossed axes, the abaxial-adaxial and the lateral axes. The PRESSED FLOWER (PRS) gene, the expression and function of which are dependent on the lateral axis, is reported in this study. In the prs mutant, growth of the lateral sep...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.931001
更新日期:2001-12-15 00:00:00
abstract::A novel 15-kDa protein, RbfA, has been identified by virtue of its ability to act as a high copy suppressor of a previously characterized dominant cold-sensitive mutation (C23U) in 16S rRNA. RbfA is found associated with free 30S ribosomal subunits, but not with 70S ribosomes or polysomes, and is essential for maximal...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.9.5.626
更新日期:1995-03-01 00:00:00
abstract::In response to skeletal muscle injury, satellite cells, which function as a myogenic stem cell population, become activated, expand through proliferation, and ultimately fuse with each other and with damaged myofibers to promote muscle regeneration. Here, we show that members of the Myocardin family of transcriptional...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.179663.111
更新日期:2012-01-15 00:00:00
abstract::The recombinational enhancer of the Hin inversion system in Salmonella stimulates recombination in vitro 150-fold in the presence of the Escherichia coli host factor Fis. To gain an understanding of the roles of the enhancer and Fis in stimulating the Hin-mediated inversion reaction, we have used nuclease and chemical...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.1.8.762
更新日期:1987-10-01 00:00:00
abstract::Atypical teratoid rhabdoid tumors (ATRTs) are challenging pediatric brain cancers that are predominantly associated with inactivation of the gene SMARCB1, a conserved subunit of the chromatin remodeling BAF complex, which has known contributions to developmental processes. To identify potential interactions between SM...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.339978.120
更新日期:2020-10-01 00:00:00
abstract::Chromatin-modifying enzymes are known to be critical components for the correct differentiation of embryonic stem cells into specific lineages, such as neurons. Recently, the role of Polycomb group proteins has been studied in the specification and differentiation of muscle stem cells. In this perspective, we review a...
journal_title:Genes & development
pub_type: 评论,杂志文章
doi:10.1101/gad.2054311
更新日期:2011-05-15 00:00:00
abstract::The removal of the 5' cap structure by the decapping enzyme DCP2 inhibits translation and generally commits the mRNA to irreversible 5'-to-3' exonucleolytic degradation by XRN1. DCP2 catalytic activity is stimulated by DCP1, and these proteins form the conserved core of the decapping complex. Additional decapping fact...
journal_title:Genes & development
pub_type: 杂志文章,评审
doi:10.1101/gad.227843.113
更新日期:2013-12-15 00:00:00
abstract::The transcription factor Ebf1 is an important determinant of early B lymphopoiesis. To gain insight into the functions of Ebf1 at distinct stages of differentiation, we conditionally inactivated Ebf1. We found that Ebf1 is required for the proliferation, survival, and signaling of pro-B cells and peripheral B-cell sub...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.187328.112
更新日期:2012-04-01 00:00:00
abstract::We have shown that Wnt signals are necessary and sufficient for neural crest cells to adopt pigment cell fates. nacre, a zebrafish homolog of MITF, is required for pigment cell differentiation. We isolated a promoter region of nacre that contains Tcf/Lef binding sites, which can mediate Wnt responsiveness. This promot...
journal_title:Genes & development
pub_type: 杂志文章
doi:
更新日期:2000-01-15 00:00:00
abstract::Dynamic assembly and disassembly of actin filaments is a major driving force for cell movements. Border cells in the Drosophila ovary provide a simple and genetically tractable model to study the mechanisms regulating cell migration. To identify new genes that regulate cell movement in vivo, we screened lethal mutatio...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.2028611
更新日期:2011-04-01 00:00:00
abstract::The p53 tumor suppressor activates either cell cycle arrest or apoptosis in response to cellular stress. Mouse embryo fibroblasts (MEFs) provide a powerful primary cell system to study both p53-dependent pathways. Specifically, in response to DNA damage, MEFs undergo p53-dependent G(1) arrest, whereas MEFs expressing ...
journal_title:Genes & development
pub_type: 杂志文章
doi:
更新日期:2000-03-15 00:00:00
abstract::The visual system is a powerful model for probing the development, connectivity, and function of neural circuits. Two genetically tractable species, mice and flies, are together providing a great deal of understanding of these processes. Current efforts focus on integrating knowledge gained from three cross-fostering ...
journal_title:Genes & development
pub_type: 杂志文章,评审
doi:10.1101/gad.248245.114
更新日期:2014-12-01 00:00:00
abstract::Human TFIIA activity is composed of three subunits (alpha, beta, gamma). Here we report the isolation of a human cDNA clone encoding the gamma-subunit and the reconstitution of TFIIA activity from recombinant polypeptides (holo-TFIIA). Protein-protein interaction analysis established that the beta and gamma subunits o...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.8.19.2336
更新日期:1994-10-01 00:00:00
abstract::The ultraviolet (UV)-induced (6-4) pyrimidine-pyrimidone photoproduct [(6-4) PP] confers a large structural distortion in DNA. Here we examine in human cells the roles of translesion synthesis (TLS) DNA polymerases (Pols) in promoting replication through a (6-4) TT photoproduct carried on a duplex plasmid where bidire...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.1872810
更新日期:2010-01-15 00:00:00
abstract::The Komeda miniature rat Ishikawa (KMI) is a naturally occurring mutant caused by an autosomal recessive mutation mri, which exhibits longitudinal growth retardation. Here we identified the mri mutation as a deletion in the rat gene encoding cGMP-dependent protein kinase type II (cGKII). KMIs showed an expanded growth...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.1224204
更新日期:2004-10-01 00:00:00