当前位置： SCI文献检索 > GENES & DEVELOPMENT期刊下所有文献 > A two-base change in a POU factor-binding site switches pituitary-specific to lymphoid-specific gene expression.

A two-base change in a POU factor-binding site switches pituitary-specific to lymphoid-specific gene expression.

Abstract：

:The structurally related POU homeo domain proteins Pit-1 and Oct-2 activate pituitary- and lymphoid-specific transcription, respectively, by binding to similar AT-rich motifs in their target genes. In this study we identify bases critical for recognition and activation by Pit-1 and examine how small differences in Pit-1 and Oct-2-binding sites can impart differential transcriptional responses in pituitary and B-lymphoid cells. Scanning mutagenesis of Pit-1 response elements in both the rat prolactin and growth hormone genes reveals a critical binding motif recognized in an identical manner by the native Pit-1 protein and cloned Pit-1 gene product. This motif, ATTATTCCAT, differs by only two bases from the octamer element, ATTTGCAT, required for Oct-2-dependent activation of immunoglobulin genes. Cross recognition of Pit-1 and Oct-2 sites by both factors can be demonstrated in competitive binding assays, in which an oligometric Pit-1 site from the prolactin gene is converted to an Oct-2 site by a double point mutation. In contrast to the binding data, no cross activation of transcription is detectable in cultured cell lines. When inserted immediately 5' to a prolactin TATA box, the wild-type prolactin element enhances transcription strongly in pituitary cells but is inactive in B cells, whereas the octamer variant of the prolactin site activates expression in B cells but is silent in pituitary lines. Both elements are nonfunctional in heterologous cell lines that lack Pit-1 and Oct-2.(ABSTRACT TRUNCATED AT 250 WORDS)

journal_name

Genes Dev

journal_title

Genes & development

authors

Elsholtz HP,Albert VR,Treacy MN,Rosenfeld MG

doi

10.1101/gad.4.1.43

subject

Has Abstract

pub_date

1990-01-01 00:00:00

pages

43-51

issue

1

eissn

0890-9369

issn

1549-5477

journal_volume

4

pub_type

杂志文章

相关文献

GENES & DEVELOPMENT文献大全
  • WD40 protein FBW5 promotes ubiquitination of tumor suppressor TSC2 by DDB1-CUL4-ROC1 ligase.

    abstract：:Tuberous sclerosis (TSC) is an autosomal dominant disease characterized by hamartoma formation in various organs and is caused by mutations targeting either the TSC1 or TSC2 genes. TSC1 and TSC2 proteins form a functionally interdependent dimeric complex. Phosphorylation of either TSC subunit by different kinases regu...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.1624008

    authors： Hu J,Zacharek S,He YJ,Lee H,Shumway S,Duronio RJ,Xiong Y

    更新日期：2008-04-01 00:00:00

  • The Arabidopsis BODENLOS gene encodes an auxin response protein inhibiting MONOPTEROS-mediated embryo patterning.

    abstract：:Developmental responses to the plant hormone auxin are thought to be mediated by interacting pairs from two protein families: short-lived inhibitory IAA proteins and ARF transcription factors binding to auxin-response elements. monopteros mutants lacking activating ARF5 and the auxin-insensitive mutant bodenlos fail t...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.229402

    authors： Hamann T,Benkova E,Bäurle I,Kientz M,Jürgens G

    更新日期：2002-07-01 00:00:00

  • Zeppo1 is a novel metastasis promoter that represses E-cadherin expression and regulates p120-catenin isoform expression and localization.

    abstract：:Amplification of 8p11-12 in human breast cancers is associated with increased proliferation and tumor grade and reduced metastasis-free patient survival. We identified Zeppo1 (zinc finger elbow-related proline domain protein 1) (FLJ14299/ZNF703) within this amplicon as a regulator of cell adhesion, migration, and prol...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.1998111

    authors： Slorach EM,Chou J,Werb Z

    更新日期：2011-03-01 00:00:00

  • I-Rel: a novel rel-related protein that inhibits NF-kappa B transcriptional activity.

    abstract：:The NF-kappa B transcription factor complex is comprised of two subunits, p50 and p65, that share significant homology to the rel oncogene. We have isolated a cDNA encoding a novel 66-kD rel-related protein, designated I-Rel. Unlike other rel-related proteins, I-Rel does not interact with DNA. I-Rel forms heterodimers...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.6.5.745

    authors： Ruben SM,Klement JF,Coleman TA,Maher M,Chen CH,Rosen CA

    更新日期：1992-05-01 00:00:00

  • Mice lacking progesterone receptor exhibit pleiotropic reproductive abnormalities.

    abstract：:Although progesterone has been recognized as essential for the establishment and maintenance of pregnancy, this steroid hormone has been recently implicated to have a functional role in a number of other reproductive events. The physiological effects of progesterone are mediated by the progesterone receptor (PR), a me...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.9.18.2266

    authors： Lydon JP,DeMayo FJ,Funk CR,Mani SK,Hughes AR,Montgomery CA Jr,Shyamala G,Conneely OM,O'Malley BW

    更新日期：1995-09-15 00:00:00

  • Suppression of cap-dependent translation in mitosis.

    abstract：:Cap-dependent translation is mediated by eIF4F, a protein complex composed of three subunits as follows: eIF4E, which recognizes the mRNA 5' cap structure; eIF4A, an RNA-helicase; and eIF4G, a scaffolding protein that binds eIF4E, eIF4A, and the eIF4E-kinase Mnk1 simultaneously. eIF4E is hypophosphorylated and cap-dep...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.889201

    authors： Pyronnet S,Dostie J,Sonenberg N

    更新日期：2001-08-15 00:00:00

  • WUSCHEL is a primary target for transcriptional regulation by SPLAYED in dynamic control of stem cell fate in Arabidopsis.

    abstract：:SNF2 chromatin-remodeling ATPases play an important role in ensuring proper development in higher eukaryotes by controlling accessibility of cis-regulatory DNA regions to transcription factors and to the transcriptional machinery. However, the biological targets controlled by these ATPases are largely unknown. Using g...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.1276305

    authors： Kwon CS,Chen C,Wagner D

    更新日期：2005-04-15 00:00:00

  • Reciprocal activation of xenobiotic response genes by nuclear receptors SXR/PXR and CAR.

    abstract：:The cytochrome P450 (CYP) gene products such as CYP3A and CYP2B are essential for the metabolism of steroid hormones and xenochemicals including prescription drugs. Nuclear receptor SXR/PXR (steroid and xenobiotic receptor/pregnenolone X receptor) has been shown both biochemically and genetically to activate CYP3A gen...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.846800

    authors： Xie W,Barwick JL,Simon CM,Pierce AM,Safe S,Blumberg B,Guzelian PS,Evans RM

    更新日期：2000-12-01 00:00:00

  • Polar localization of the replication origin and terminus in Escherichia coli nucleoids during chromosome partitioning.

    abstract：:We show the intracellular localization of the Escherichia coli replication origin (oriC) and chromosome terminus during the cell division cycle by FISH. In newborn cells, oriC is localized at the old-pole-proximal nucleoid border and the terminus at the new-pole-proximal nucleoid border. One copy of replicated oriC mi...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.12.7.1036

    authors： Niki H,Hiraga S

    更新日期：1998-04-01 00:00:00

  • Nascent peptide-mediated translation elongation arrest coupled with mRNA degradation in the CGS1 gene of Arabidopsis.

    abstract：:Expression of the Arabidopsis CGS1 gene that codes for cystathionine gamma-synthase is feedback regulated at the step of mRNA stability in response to S-adenosyl-L-methionine (AdoMet). A short stretch of amino acid sequence, called the MTO1 region, encoded by the first exon of CGS1 itself is involved in this regulatio...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.1317105

    authors： Onouchi H,Nagami Y,Haraguchi Y,Nakamoto M,Nishimura Y,Sakurai R,Nagao N,Kawasaki D,Kadokura Y,Naito S

    更新日期：2005-08-01 00:00:00

  • Pioneer-like factor GAF cooperates with PBAP (SWI/SNF) and NURF (ISWI) to regulate transcription.

    abstract：:Transcriptionally silent genes must be activated throughout development. This requires nucleosomes be removed from promoters and enhancers to allow transcription factor (TF) binding and recruitment of coactivators and RNA polymerase II (Pol II). Specialized pioneer TFs bind nucleosome-wrapped DNA to perform this chrom...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.341768.120

    authors： Judd J,Duarte FM,Lis JT

    更新日期：2021-01-01 00:00:00

  • Breaking down bone: new insight into site-specific mechanisms of breast cancer osteolysis mediated by metalloproteinases.

    abstract：:Bone metastases are the most common skeletal complication of malignancy. Tumor cells disrupt normal bone remodeling to promote bone destruction and its associated morbidity. In the August 15, 2009, issue of Genes & Development, Lu and colleagues (pp. 1882-1894) propose a novel molecular mechanism by which tumor-produc...

    journal_title：Genes & development

    pub_type： 评论,杂志文章

    doi：10.1101/gad.1854909

    authors： Guise TA

    更新日期：2009-09-15 00:00:00

  • A Polycomb group protein complex with sequence-specific DNA-binding and selective methyl-lysine-binding activities.

    abstract：:Polycomb response elements (PREs) are specific cis-regulatory sequences needed for transcriptional repression of HOX and other target genes by Polycomb group (PcG) proteins. Among the many PcG proteins known in Drosophila, Pho is the only sequence-specific DNA-binding protein. To gain insight into the function of Pho,...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.377406

    authors： Klymenko T,Papp B,Fischle W,Köcher T,Schelder M,Fritsch C,Wild B,Wilm M,Müller J

    更新日期：2006-05-01 00:00:00

  • A splicing enhancer exhibits both constitutive and regulated activities.

    abstract：:The Drosophila proteins Transformer (Tra) and Transformer2 (Tra2) regulate the sex-specific alternative splicing of Drosophila doublesex (dsx) pre-mRNA by specifically binding to a splicing enhancer (dsx repeat element; dsxRE) located 300 nucleotides (nt) downstream from a female-specific 3' splice site. In this paper...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.8.14.1703

    authors： Tian M,Maniatis T

    更新日期：1994-07-15 00:00:00

  • Oscillations of MyoD and Hes1 proteins regulate the maintenance of activated muscle stem cells.

    abstract：:The balance between proliferation and differentiation of muscle stem cells is tightly controlled, ensuring the maintenance of a cellular pool needed for muscle growth and repair. We demonstrate here that the transcriptional regulator Hes1 controls the balance between proliferation and differentiation of activated musc...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.322818.118

    authors： Lahmann I,Bröhl D,Zyrianova T,Isomura A,Czajkowski MT,Kapoor V,Griger J,Ruffault PL,Mademtzoglou D,Zammit PS,Wunderlich T,Spuler S,Kühn R,Preibisch S,Wolf J,Kageyama R,Birchmeier C

    更新日期：2019-05-01 00:00:00

  • FILAMENTOUS FLOWER, a meristem and organ identity gene of Arabidopsis, encodes a protein with a zinc finger and HMG-related domains.

    abstract：:Distinctive from that of the animal system, the basic plan of the plant body is the continuous formation of a structural unit, composed of a stem with a meristem at the top and lateral organs continuously forming at the meristem. Therefore, mechanisms controlling the formation, maintenance, and development of a merist...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.13.9.1079

    authors： Sawa S,Watanabe K,Goto K,Liu YG,Shibata D,Kanaya E,Morita EH,Okada K

    更新日期：1999-05-01 00:00:00

  • The regulation of empty spiracles by Abdominal-B mediates an abdominal segment identity function.

    abstract：:The empty spiracles (ems) homeo box gene is required for the development of the Drosophila larval filzkörper, which are structural specializations of the eighth abdominal segment. Filzkörper development is also dependent on the function of the homeotic selector gene Abdominal-B (Abd-B). Here, we show that ems is a dow...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.7.2.229

    authors： Jones B,McGinnis W

    更新日期：1993-02-01 00:00:00

  • Cell fusion induced by ERVWE1 or measles virus causes cellular senescence.

    abstract：:Cellular senescence limits proliferation of potentially detrimental cells, preventing tumorigenesis and restricting tissue damage. However, the function of senescence in nonpathological conditions is unknown. We found that the human placental syncytiotrophoblast exhibited the phenotype and expressed molecular markers ...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.227512.113

    authors： Chuprin A,Gal H,Biron-Shental T,Biran A,Amiel A,Rozenblatt S,Krizhanovsky V

    更新日期：2013-11-01 00:00:00

  • A genetic screen identifies the Triple T complex required for DNA damage signaling and ATM and ATR stability.

    abstract：:In response to DNA damage, cells activate a complex signal transduction network called the DNA damage response (DDR). To enhance our current understanding of the DDR network, we performed a genome-wide RNAi screen to identify genes required for resistance to ionizing radiation (IR). Along with a number of known DDR ge...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.1934210

    authors： Hurov KE,Cotta-Ramusino C,Elledge SJ

    更新日期：2010-09-01 00:00:00

  • Quantitative epigenetics: DNA sequence variation need not apply.

    abstract：:Two recent reports, including one by Reinders and colleagues (pp. 939-950) in the April 15, 2009, issue of Genes & Development, describe the construction of Arabidopsis recombinant inbred populations that maximize epigenetic rather than genetic variation. The distribution and behavior of phenotypic variation in these ...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.1824909

    authors： Richards EJ

    更新日期：2009-07-15 00:00:00

  • A new regulatory protein, KSRP, mediates exon inclusion through an intronic splicing enhancer.

    abstract：:We have purified and cloned a new splicing factor, KSRP. KSRP is a component of a multiprotein complex that binds specifically to an intronic splicing enhancer element downstream of the neuron-specific c-src N1 exon. This 75-kD protein induces the assembly of five other proteins, including the heterogeneous nuclear ri...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.11.8.1023

    authors： Min H,Turck CW,Nikolic JM,Black DL

    更新日期：1997-04-15 00:00:00

  • Smad3-dependent nuclear translocation of beta-catenin is required for TGF-beta1-induced proliferation of bone marrow-derived adult human mesenchymal stem cells.

    abstract：:Adult mesenchymal stem cells (MSCs) derived from bone marrow contribute to the regeneration of multiple types of mesenchymal tissues. Here we describe the functional role of a novel form of cross-talk between the transforming growth factor beta1 (TGF-beta1) and Wnt signaling pathways in regulating the activities of hu...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.1388806

    authors： Jian H,Shen X,Liu I,Semenov M,He X,Wang XF

    更新日期：2006-03-15 00:00:00

  • STAU1 binding 3' UTR IRAlus complements nuclear retention to protect cells from PKR-mediated translational shutdown.

    abstract：:For a number of human genes that encode transcripts containing inverted repeat Alu elements (IRAlus) within their 3' untranslated region (UTR), product mRNA is efficiently exported to the cytoplasm when the IRAlus, which mediate nuclear retention, are removed by alternative polyadenylation. Here we report a new mechan...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.220962.113

    authors： Elbarbary RA,Li W,Tian B,Maquat LE

    更新日期：2013-07-01 00:00:00

  • Chromatin signatures of cancer.

    abstract：:Changes in the pattern of gene expression play an important role in allowing cancer cells to acquire their hallmark characteristics, while genomic instability enables cells to acquire genetic alterations that promote oncogenesis. Chromatin plays central roles in both transcriptional regulation and the maintenance of g...

    journal_title：Genes & development

    pub_type： 杂志文章,评审

    doi：10.1101/gad.255182.114

    authors： Morgan MA,Shilatifard A

    更新日期：2015-02-01 00:00:00

  • Intercellular C-signaling in Myxococcus xanthus involves a branched signal transduction pathway.

    abstract：:C-factor, the product of the csgA gene, is a cell-surface associated short-range intercellular signaling protein in Myxococcus xanthus. C-factor is required for at least four responses during starvation-induced fruiting body morphogenesis: rippling, aggregation, sporulation, and full expression of the csgA gene, all o...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.10.6.740

    authors： Søgaard-Andersen L,Slack FJ,Kimsey H,Kaiser D

    更新日期：1996-03-15 00:00:00

  • Removal of maternal retinoic acid by embryonic CYP26 is required for correct Nodal expression during early embryonic patterning.

    abstract：:The abundance of retinoic acid (RA) is determined by the balance between its synthesis by retinaldehyde dehydrogenase (RALDH) and its degradation by CYP26. In particular, the dynamic expression of three CYP26 genes controls the regional level of RA within the body. Pregastrulation mouse embryos express CYP26 but not R...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.1776209

    authors： Uehara M,Yashiro K,Takaoka K,Yamamoto M,Hamada H

    更新日期：2009-07-15 00:00:00

  • The Drosophila RNA-binding protein RBP1 is localized to transcriptionally active sites of chromosomes and shows a functional similarity to human splicing factor ASF/SF2.

    abstract：:An RNA-binding protein gene (rbp1) from Drosophila melanogaster, encoding an RNA recognition motif and an Arg-Ser rich (RS) domain, has been characterized. The predicted amino acid sequence of rbp1 is similar to those of the human splicing factor ASF/SF2, the Drosophila nuclear phosphoprotein SRp55, and the Drosophila...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.6.12b.2569

    authors： Kim YJ,Zuo P,Manley JL,Baker BS

    更新日期：1992-12-01 00:00:00

  • Tandem AP-1-binding sites within the human beta-globin dominant control region function as an inducible enhancer in erythroid cells.

    abstract：:A powerful enhancer has been mapped to an 18-bp DNA segment located 11 kb 5' to the human epsilon-globin gene within the dominant control or locus-activating region. This enhancer is inducible in K562 human erythroleukemia cells, increasing linked gamma-globin promoter/luciferase gene expression to 170-fold over an en...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.4.6.993

    authors： Ney PA,Sorrentino BP,McDonagh KT,Nienhuis AW

    更新日期：1990-06-01 00:00:00

  • Structures of three distinct activator-TFIID complexes.

    abstract：:Sequence-specific DNA-binding activators, key regulators of gene expression, stimulate transcription in part by targeting the core promoter recognition TFIID complex and aiding in its recruitment to promoter DNA. Although it has been established that activators can interact with multiple components of TFIID, it is unk...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.1790709

    authors： Liu WL,Coleman RA,Ma E,Grob P,Yang JL,Zhang Y,Dailey G,Nogales E,Tjian R

    更新日期：2009-07-01 00:00:00

  • Enzymatic regulation of pattern: BMP4 binds CUB domains of Tolloids and inhibits proteinase activity.

    abstract：:In Xenopus embryos, a dorsal-ventral patterning gradient is generated by diffusing Chordin/bone morphogenetic protein (BMP) complexes cleaved by BMP1/Tolloid metalloproteinases in the ventral side. We developed a new BMP1/Tolloid assay using a fluorogenic Chordin peptide substrate and identified an unexpected negative...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.1839309

    authors： Lee HX,Mendes FA,Plouhinec JL,De Robertis EM

    更新日期：2009-11-01 00:00:00