Novel murine homeo box gene on chromosome 1 expressed in specific hematopoietic lineages and during embryogenesis.


:We describe here a new murine homeo box gene, denoted Hlx, which is expressed within specific hematopoietic lineages. The cDNA sequence indicates that Hlx differs markedly from known vertebrate homeo box genes, and linkage analysis of an interspecific murine backcross showed that it resides at a novel homeo box locus on the distal portion of mouse chromosome 1. The Hlx homeo domain is most similar to that of the Drosophila H2.0 gene, but outside this region the two polypeptides are related only within a few short segments, the most notable being a motif (denoted Hep) also partially conserved in the engrailed and invected homeo proteins and possibly related to an octapeptide in certain paired box proteins. The presence of an intron within the Hlx homeo box at the same position as in several divergent Drosophila genes (H2.0, labial, Distal-less, proboscipedia, Abdominal-B, NK-1) suggests an ancient evolutionary relationship between these genes. RNA analysis of 67 murine hematopoietic cell lines and normal hematopoietic cells revealed Hlx expression throughout the myeloid/macrophage lineage and at early stages of B lymphocyte development but not in T lymphocytes, erythroid cells, or mast cells. Hence, Hlx is a candidate regulator of hematopoietic lineage commitment and maturation. It probably also functions outside the hematopoietic system, however, because Hlx mRNA could be detected in diverse adult tissues and in embryos from as early as day 8 of development.


Genes Dev


Genes & development


Allen JD,Lints T,Jenkins NA,Copeland NG,Strasser A,Harvey RP,Adams JM




Has Abstract


1991-04-01 00:00:00












  • Activator recruitment by the general transcription machinery: X-ray structural analysis of the Oct-1 POU domain/human U1 octamer/SNAP190 peptide ternary complex.

    abstract::Transcriptional activation of the human U1 snRNA genes is dependent on a noncanonical octamer element contained within an upstream enhancer. The U1 octamer only weakly recruits the Oct-1 POU domain, although recruitment is stimulated by a peptide containing the Oct-1-binding domain of SNAP190. Structural analysis of t...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Hovde S,Hinkley CS,Strong K,Brooks A,Gu L,Henry RW,Geiger J

    更新日期:2002-11-01 00:00:00

  • A splicing enhancer exhibits both constitutive and regulated activities.

    abstract::The Drosophila proteins Transformer (Tra) and Transformer2 (Tra2) regulate the sex-specific alternative splicing of Drosophila doublesex (dsx) pre-mRNA by specifically binding to a splicing enhancer (dsx repeat element; dsxRE) located 300 nucleotides (nt) downstream from a female-specific 3' splice site. In this paper...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Tian M,Maniatis T

    更新日期:1994-07-15 00:00:00

  • Expression of a dominant-negative Wnt blocks induction of MyoD in Xenopus embryos.

    abstract::During gastrulation of Xenopus embryos the prospective mesoderm is induced initially with domains of dorsal and ventral fate, then further patterned to generate somitic mesoderm by signals from the gastrula organizer. Although Xwnt-8 is expressed in future ventrolateral mesoderm and induces prospective epidermis to di...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Hoppler S,Brown JD,Moon RT

    更新日期:1996-11-01 00:00:00

  • Meiotic double-strand breaks at the interface of chromosome movement, chromosome remodeling, and reductional division.

    abstract::Chromosomal processes related to formation and function of meiotic chiasmata have been analyzed in Sordaria macrospora. Double-strand breaks (DSBs), programmed or gamma-rays-induced, are found to promote four major events beyond recombination and accompanying synaptonemal complex formation: (1) juxtaposition of homolo...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Storlazzi A,Tessé S,Gargano S,James F,Kleckner N,Zickler D

    更新日期:2003-11-01 00:00:00

  • VHL-box and SOCS-box domains determine binding specificity for Cul2-Rbx1 and Cul5-Rbx2 modules of ubiquitin ligases.

    abstract::The ECS (Elongin B/C-Cul2/Cul5-SOCS-box protein) complex is a member of a family of ubiquitin ligases that share a Cullin-Rbx module. SOCS-box proteins recruit substrates to the ECS complex and are linked to Cullin-Rbx via Elongin B/C. VHL has been implicated as a SOCS-box protein, but lacks a C-terminal sequence (dow...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Kamura T,Maenaka K,Kotoshiba S,Matsumoto M,Kohda D,Conaway RC,Conaway JW,Nakayama KI

    更新日期:2004-12-15 00:00:00

  • ZNF281 enhances cardiac reprogramming by modulating cardiac and inflammatory gene expression.

    abstract::Direct reprogramming of fibroblasts to cardiomyocytes represents a potential means of restoring cardiac function following myocardial injury. AKT1 in the presence of four cardiogenic transcription factors, GATA4, HAND2, MEF2C, and TBX5 (AGHMT), efficiently induces the cardiac gene program in mouse embryonic fibroblast...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Zhou H,Morales MG,Hashimoto H,Dickson ME,Song K,Ye W,Kim MS,Niederstrasser H,Wang Z,Chen B,Posner BA,Bassel-Duby R,Olson EN

    更新日期:2017-09-01 00:00:00

  • Suppression of cap-dependent translation in mitosis.

    abstract::Cap-dependent translation is mediated by eIF4F, a protein complex composed of three subunits as follows: eIF4E, which recognizes the mRNA 5' cap structure; eIF4A, an RNA-helicase; and eIF4G, a scaffolding protein that binds eIF4E, eIF4A, and the eIF4E-kinase Mnk1 simultaneously. eIF4E is hypophosphorylated and cap-dep...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Pyronnet S,Dostie J,Sonenberg N

    更新日期:2001-08-15 00:00:00

  • Identification of a locus control region in the immunoglobulin heavy-chain locus that deregulates c-myc expression in plasmacytoma and Burkitt's lymphoma cells.

    abstract::In murine plasmacytoma and human Burkitt's lymphoma cells, one allele of c-myc is translocated into one of the immunoglobulin loci, resulting in a characteristic pattern of deregulated c-myc transcription. Translocation events between c-myc and the IgH locus segregate c-myc and the IgH intron enhancer to different rec...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Madisen L,Groudine M

    更新日期:1994-09-15 00:00:00

  • Reciprocal activation of xenobiotic response genes by nuclear receptors SXR/PXR and CAR.

    abstract::The cytochrome P450 (CYP) gene products such as CYP3A and CYP2B are essential for the metabolism of steroid hormones and xenochemicals including prescription drugs. Nuclear receptor SXR/PXR (steroid and xenobiotic receptor/pregnenolone X receptor) has been shown both biochemically and genetically to activate CYP3A gen...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Xie W,Barwick JL,Simon CM,Pierce AM,Safe S,Blumberg B,Guzelian PS,Evans RM

    更新日期:2000-12-01 00:00:00

  • A model for the abrogation of the SOS response by an SOS protein: a negatively charged helix in DinI mimics DNA in its interaction with RecA.

    abstract::DinI is a recently described negative regulator of the SOS response in Escherichia coli. Here we show that it physically interacts with RecA and prevents the binding of single-stranded DNA to RecA, which is required for the activation of the latter. DinI also displaces ssDNA from a stable RecA-DNA cofilament, thus eli...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Voloshin ON,Ramirez BE,Bax A,Camerini-Otero RD

    更新日期:2001-02-15 00:00:00

  • Disruption of retinoblastoma protein function by coexpression of its C pocket fragment.

    abstract::The growth suppression function of the retinoblastoma protein (RB) is mediated by its interaction with a variety of cellular proteins. RB contains at least two protein-binding pockets: the large A/B pocket, which interacts with E2F and the D-type cyclins, and the C pocket, which interacts with the nuclear c-Abl tyrosi...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Welch PJ,Wang JY

    更新日期:1995-01-01 00:00:00

  • Nuclear receptor-like transcription factors in fungi.

    abstract::Members of the metazoan nuclear receptor superfamily regulate gene expression programs in response to binding of cognate lipophilic ligands. Evolutionary studies using bioinformatics tools have concluded that lower eukaryotes, such as fungi, lack nuclear receptor homologs. Here we review recent discoveries suggesting ...

    journal_title:Genes & development

    pub_type: 杂志文章,评审


    authors: Näär AM,Thakur JK

    更新日期:2009-02-15 00:00:00

  • A new regulatory protein, KSRP, mediates exon inclusion through an intronic splicing enhancer.

    abstract::We have purified and cloned a new splicing factor, KSRP. KSRP is a component of a multiprotein complex that binds specifically to an intronic splicing enhancer element downstream of the neuron-specific c-src N1 exon. This 75-kD protein induces the assembly of five other proteins, including the heterogeneous nuclear ri...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Min H,Turck CW,Nikolic JM,Black DL

    更新日期:1997-04-15 00:00:00

  • Modulating the outer membrane with small RNAs.

    abstract::MicF, one of the first chromosomally encoded regulatory small RNAs (sRNAs) to be discovered, was found to modulate the expression of OmpF, an abundant outer membrane protein. Several recent papers have now shown that this is not an isolated case. At least five other sRNAs also regulate the synthesis of outer membrane ...

    journal_title:Genes & development

    pub_type: 杂志文章,评审


    authors: Guillier M,Gottesman S,Storz G

    更新日期:2006-09-01 00:00:00

  • The relative ratio of condensin I to II determines chromosome shapes.

    abstract::To understand how chromosome shapes are determined by actions of condensins and cohesin, we devised a series of protocols in which their levels are precisely changed in Xenopus egg extracts. When the relative ratio of condensin I to II is forced to be smaller, embryonic chromosomes become shorter and thicker, being re...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Shintomi K,Hirano T

    更新日期:2011-07-15 00:00:00

  • A Drosophila rRNA gene located in euchromatin is active in transcription and nucleolus formation.

    abstract::P-element transformants of a single rRNA gene (rDNA) were used to investigate the relationship between the organization of the nucleolus organizer (NO) and rDNA function in Drosophila melanogaster. In situ hybridization to rRNA in polytene nuclei of salivary glands demonstrated that an rRNA gene can be transcribed at ...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Karpen GH,Schaefer JE,Laird CD

    更新日期:1988-12-01 00:00:00

  • Mechanistic insights and identification of two novel factors in the C. elegans NMD pathway.

    abstract::The nonsense-mediated mRNA decay (NMD) pathway selectively degrades mRNAs harboring premature termination codons (PTCs). Seven genes (smg-1-7, for suppressor with morphological effect on genitalia) that are essential for NMD were originally identified in the nematode Caenorhabditis elegans, and orthologs of these gene...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Longman D,Plasterk RH,Johnstone IL,Cáceres JF

    更新日期:2007-05-01 00:00:00

  • DNA damage activates p53 through a phosphorylation-acetylation cascade.

    abstract::Activation of p53-mediated transcription is a critical cellular response to DNA damage. p53 stability and site-specific DNA-binding activity and, therefore, transcriptional activity, are modulated by post-translational modifications including phosphorylation and acetylation. Here we show that p53 is acetylated in vitr...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Sakaguchi K,Herrera JE,Saito S,Miki T,Bustin M,Vassilev A,Anderson CW,Appella E

    更新日期:1998-09-15 00:00:00

  • Structure and function of the PWI motif: a novel nucleic acid-binding domain that facilitates pre-mRNA processing.

    abstract::The PWI motif is a highly conserved domain of unknown function in the SRm160 splicing and 3'-end cleavage-stimulatory factor, as well as in several other known or putative pre-mRNA processing components. We show here that the PWI motif is a new type of RNA/DNA-binding domain that has an equal preference for single- an...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Szymczyna BR,Bowman J,McCracken S,Pineda-Lucena A,Lu Y,Cox B,Lambermon M,Graveley BR,Arrowsmith CH,Blencowe BJ

    更新日期:2003-02-15 00:00:00

  • The Nf2 tumor suppressor gene product is essential for extraembryonic development immediately prior to gastrulation.

    abstract::The neurofibromatosis type II (NF2) tumor suppressor encodes a putative cytoskeletal associated protein, the loss of which leads to the development of Schwann cell tumors associated with NF2 in humans. The NF2 protein merlin belongs to the band 4.1 family of proteins that link membrane proteins to the cytoskeleton and...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: McClatchey AI,Saotome I,Ramesh V,Gusella JF,Jacks T

    更新日期:1997-05-15 00:00:00

  • Seven-up, the Drosophila homolog of the COUP-TF orphan receptors, controls cell proliferation in the insect kidney.

    abstract::Morphogenesis of the insect kidney, the Malpighian tubules, is controlled in Drosophila by a single large cell, the tip cell. It has been postulated that this cell sends out a mitogenic signal that induces the division of neighboring cells. The signal and the molecules that receive it have remained elusive. We show th...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Kerber B,Fellert S,Hoch M

    更新日期:1998-06-15 00:00:00

  • The Neurospora circadian clock-controlled gene, ccg-2, is allelic to eas and encodes a fungal hydrophobin required for formation of the conidial rodlet layer.

    abstract::The Neurospora crassa clock-controlled gene (ccg-2) is transcriptionally activated by the circadian clock in a time-of-day-specific manner. Transcript and sequence analyses of ccg-2 reveal that the predicted Ccg-2 polypeptide bears significant similarity to a class of low-molecular-weight, cysteine-rich, hydrophobic p...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Bell-Pedersen D,Dunlap JC,Loros JJ

    更新日期:1992-12-01 00:00:00

  • Epigenetic effects on yeast transcription caused by mutations in an actin-related protein present in the nucleus.

    abstract::Gene inactivation can result when a delta element of the Ty1 transposon inserted into the yeast HIS4 promoter (his4-912delta) alters the transcription initiation site. Previous work has identified mutations that suppress this transcriptional defect by restoring the transcription start site to the native position, and ...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Jiang YW,Stillman DJ

    更新日期:1996-03-01 00:00:00

  • PI3K pathway regulates survival of cancer stem cells residing in the perivascular niche following radiation in medulloblastoma in vivo.

    abstract::Medulloblastomas are brain tumors that arise in the cerebellum of children and contain stem cells in a perivascular niche thought to give rise to recurrence following radiation. We used several mouse models of medulloblastomas in parallel to better understand how the critical cell types in these tumors respond to ther...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Hambardzumyan D,Becher OJ,Rosenblum MK,Pandolfi PP,Manova-Todorova K,Holland EC

    更新日期:2008-02-15 00:00:00

  • APC sets the Wnt tone necessary for cerebral cortical progenitor development.

    abstract::Adenomatous polyposis coli (APC) regulates the activity of β-catenin, an integral component of Wnt signaling. However, the selective role of the APC-β-catenin pathway in cerebral cortical development is unknown. Here we genetically dissected the relative contributions of APC-regulated β-catenin signaling in cortical p...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Nakagawa N,Li J,Yabuno-Nakagawa K,Eom TY,Cowles M,Mapp T,Taylor R,Anton ES

    更新日期:2017-08-15 00:00:00

  • Mutation of the AAUAAA polyadenylation signal depresses in vitro splicing of proximal but not distal introns.

    abstract::To investigate the relationship between splicing and polyadenylation during the production of vertebrate mRNAs, we examined the effect of mutation of a poly(A) site on splicing of upstream introns. Mutation of the AAUAAA polyadenylation consensus sequence inhibited in vitro splicing of an upstream intron. The magnitud...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Niwa M,Berget SM

    更新日期:1991-11-01 00:00:00

  • Translational profiling of hypocretin neurons identifies candidate molecules for sleep regulation.

    abstract::Hypocretin (orexin; Hcrt)-containing neurons of the hypothalamus are essential for the normal regulation of sleep and wake behaviors and have been implicated in feeding, anxiety, depression, and reward. The absence of these neurons causes narcolepsy in humans and model organisms. However, little is known about the mol...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Dalal J,Roh JH,Maloney SE,Akuffo A,Shah S,Yuan H,Wamsley B,Jones WB,de Guzman Strong C,Gray PA,Holtzman DM,Heintz N,Dougherty JD

    更新日期:2013-03-01 00:00:00

  • The mouse homolog of Drosophila Vasa is required for the development of male germ cells.

    abstract::Restricted expression of a mouse Vasa homolog gene (Mvh) expression is first detected in primordial germ cells (PGCs) after colonization of the genital ridges. Subsequently, Mvh is maintained until postmeiotic germ cells are formed. Here, we demonstrate that male mice homozygous for a targeted mutation of Mvh exhibit ...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Tanaka SS,Toyooka Y,Akasu R,Katoh-Fukui Y,Nakahara Y,Suzuki R,Yokoyama M,Noce T

    更新日期:2000-04-01 00:00:00

  • The 65-kD subunit of NF-kappa B is a receptor for I kappa B and a modulator of DNA-binding specificity.

    abstract::A recent study has shown that the dimeric form of the 50-kD DNA-binding subunit of the NF-kappa B transcription factor is commonly associated with two molecules of a 65-kD protein (p65) and that p65 appears to be required for inactivation of the p50 dimer by the inhibitory subunit I kappa B. Here, we provide evidence ...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Urban MB,Baeuerle PA

    更新日期:1990-11-01 00:00:00

  • Life on the edge: telomeres and persistent DNA breaks converge at the nuclear periphery.

    abstract::Persistent DNA double-strand breaks and telomeres represent genomic hazards, as they can instigate inappropriate repair reactions. Two recent papers by Oza and colleagues (pp. 912-917) and Schober and colleagues (pp. 928-938) show that both types of DNA ends are sequestered from bulk DNA by Mps3, a SUN domain protein ...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Gartenberg MR

    更新日期:2009-05-01 00:00:00