Abstract:
:The heterochromatin-like structure formed by the yeast silent information regulator complex (SIR) represses transcription at the silent mating type loci and telomeres. Here, we report that tight protein-DNA complexes induce ectopic recruitment of the SIR complex, promoting gene silencing and changes in subnuclear localization when cis-acting elements are nearby. Importantly, lack of the replication fork-associated helicase Rrm3 enhances this induced gene repression. Additionally, Sir3 and Sir4 are enriched genome-wide at natural replication pause sites, including tRNA genes. Consistently, inserting a tRNA gene promotes SIR-mediated silencing of a nearby gene. These results reveal that replication stress arising from tight DNA-protein interactions favors heterochromatin formation.
journal_name
Genes Devjournal_title
Genes & developmentauthors
Dubarry M,Loïodice I,Chen CL,Thermes C,Taddei Adoi
10.1101/gad.611011subject
Has Abstractpub_date
2011-07-01 00:00:00pages
1365-70issue
13eissn
0890-9369issn
1549-5477pii
25/13/1365journal_volume
25pub_type
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