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Phage N4 RNA polymerase II recruitment to DNA by a single-stranded DNA-binding protein.

Abstract：

:Transcription of bacteriophage N4 middle genes is carried out by a phage-coded, heterodimeric RNA polymerase (N4 RNAPII), which belongs to the family of T7-like RNA polymerases. In contrast to phage T7-RNAP, N4 RNAPII displays no activity on double-stranded templates and low activity on single-stranded templates. In vivo, at least one additional N4-coded protein (p17) is required for N4 middle transcription. We show that N4 ORF2 encodes p17 (gp2). Characterization of purified gp2revealed that it is a single-stranded DNA-binding protein that activates N4 RNAPII transcription on single-stranded DNA templates through specific interaction with N4 RNAPII. On the basis of the properties of the proteins involved in N4 RNAPII transcription and of middle promoters, we propose a model for N4 RNAPII promoter recognition, in which gp2plays two roles, stabilization of a single-stranded region at the promoter and recruitment of N4 RNAPII through gp2-N4 RNAPII interactions. Furthermore, we discuss our results in the context of transcription initiation by mitochondrial RNA polymerases.

journal_name

Genes Dev

journal_title

Genes & development

authors

Carter RH,Demidenko AA,Hattingh-Willis S,Rothman-Denes LB

doi

10.1101/gad.1121403

subject

Has Abstract

pub_date

2003-09-15 00:00:00

pages

2334-45

issue

18

eissn

0890-9369

issn

1549-5477

pii

17/18/2334

journal_volume

17

pub_type

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